@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(1):17-21
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(1):17-21
Effect of Otostegia persica’s Root Extract on the Blood Biochemical Factors in Diabetic Hyperlipidemic Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Dourandishan M. (1)Hosseini M. (2)
Malekaneh M. (*)
Bagherzade Gh. (1)
(*) Biochemistry Department, Paramedical Faculty, Birjand University of Medical Sciences, Birjand, Iran
(1) Phytochemistry Department, Basic Sciences Faculty, Birjand University, Birjand, Iran
(2) Biochemistry Department, , , , , Paramedical Faculty, Birjand University of Medical Sciences, Birjand, Iran
Correspondence
Address: Biochemistry Department, Paramedical Faculty, Birjand University of Medical Sciences, Ayatollah Ghaffari Street, Birjand, South Khorasan, IranPhone: +985614440556
Fax: +985614440556
drmalekaneh@bums.ac.ir
Article History
Received: September 26, 2013Accepted: March 3, 2014
ePublished: March 1, 2014
ABSTRACT
Aims
Considering the multidimensional activity of herbal medicines and the usefulness
of this drugs to treat complex disorders such as diabetes, this study was done to
investigate the effect of aqueous extract of Otostegia persica’s root on blood lipids and
lipoproteins levels in hyperlipidemic diabetic type I rats.
Materials & Methods This experimental study was done on 48 male Wistar rats. All ethical issues related to the keeping and work with laboratory animals was observed. 40 rats with a single dosage of 90mg per each kilogram of body weight were induced type I diabetes by intraperitoneally injection of Alloxan. Rats were divided into 6 normal, control, metformin, 200mg, 300mg and 400mg/kg of Otostegia persica’s root aqueous extract groups each with 8 rats and they were gavaged once a day during a month. Levels of triglycerides, total cholesterol, LDL-C and HDL-C were measured in each group. The one-way ANOVA and Tukey›s tests were used to compare means of the groups.
Findings The mean of triglyceride, total cholesterol, LDL-C and HDL-C level in all three groups of Otostegia persica’s root aqueous extract compared to normal, control and metformin showed a significant decrease (p<0.001).
Conclusion Using aqueous Otostegia persica’s root extract in hyperlipidemic diabetic rats decreases serum total cholesterol, triglyceride and LDL-C level.
Materials & Methods This experimental study was done on 48 male Wistar rats. All ethical issues related to the keeping and work with laboratory animals was observed. 40 rats with a single dosage of 90mg per each kilogram of body weight were induced type I diabetes by intraperitoneally injection of Alloxan. Rats were divided into 6 normal, control, metformin, 200mg, 300mg and 400mg/kg of Otostegia persica’s root aqueous extract groups each with 8 rats and they were gavaged once a day during a month. Levels of triglycerides, total cholesterol, LDL-C and HDL-C were measured in each group. The one-way ANOVA and Tukey›s tests were used to compare means of the groups.
Findings The mean of triglyceride, total cholesterol, LDL-C and HDL-C level in all three groups of Otostegia persica’s root aqueous extract compared to normal, control and metformin showed a significant decrease (p<0.001).
Conclusion Using aqueous Otostegia persica’s root extract in hyperlipidemic diabetic rats decreases serum total cholesterol, triglyceride and LDL-C level.
CITATION LINKS
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[17]Hedayati M, Pouraboli I, Pouraboli B. Effect of methanolic extract of Otostegia persica on serum levels of glucose and lipids in type I diabetic male rats. Iran J End and Met. 2010;12(4):435-58. [Persian]
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[21]Khan S, Syed F. Bioactive Constituents from Genus Otostegia. SARJ Phy Sci. 2013;1(1):15-25.
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[23]Yamamoto Y, Oue E. Antihypertensive effect of quercetin in rats fed with a high-fat high-sucrose diet. Biosci Biotechnol Biochem. 2006;70(4):933-9.
[24]Ricardo S, Oliveira TT, Nagem TJ, Pinto AD, Oliveira MG. Effect of flavonoids morin; quercetin and nicotinic acid on lipid metabolism of rats experimentally fed with triton. Braz Arch Biol Tec. 2001;44(3):263-7.
[25]Li XM, Zhou AG. Evaluation of antioxidant activity of the poly saccharides extracted from Lycium barbarum fruits in vitro. Euro Poly J. 2007;43(2):488-97.
[2]Vozarova B, Stefan N, Lindsay RS, Saremi A, Pratley RE, Bogardus C, et al. High alanine aminotransferase is associated with decreased hepatic insulin sensitivity and predicts the development of type 2 diabetes. Diabetes. 2002;51(6):1889-95.
[3]Tannock LR. Advances in the management of hyperlipidemia-induced atherosclerosis. Expert Rev Cardiovasc Ther. 2008;6(3):369-83.
[4]Jonas A. Lipoprotein structure. In: Vance DE, Vance JE (editors). Biochemistry of Lipids, Lipoproteins and Membranes. 4th ed. Amsterdam: Elsevier: 2002. pp. 483-504.
[5]Brown MV. Lipoprotein disorders in diabetes mellitus. Med Clin North Am. 1994;78(1):143-61.
[6]James I. Executive summary of the third report of the National Cholesterol Education Program (NCEP) Expert Panel on etection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult TreatmentPanel III). JAMA. 2001;285(19):2486-97.
[7]Yadav UC, Moorthy K, Baquer NZ. Effects of sodium-orthovanadate and Trigonella foenum-graecum seeds on hepatic and renal lipogenic enzymes and lipid profile during alloxan diabetes. J Biosci. 2004;29(1):81-91.
[8]Winocour PH, Durrington PN, Bhatnagar D, Ishola M, Arrol S, Mackness M. Abnormalities of VLDL, IDL and LDL characterize insulin dependent diabetes mellitus. Arterosclerosis Thromb. 1992;12(8):920-28.
[9]Abou-Seif MA, Yussef AA. Evaluation of some biochemical changes in diabetic patients. Clin Chem Acta. 2004;346(2):161-70.
[10]Reaven GM, Johnston PETER, Hollenbeck CB, Skowronski ROMAN, Zhang, JC, Goldfine ID, et al. Combined Metformin-Sulfonylurea treatment diabetes in fair to poor glycemic Control. J clin Endo Met. 1992;74(5):1020-26.
[11]Ziyyat A, Legssyer A, Mekhfi H, Dassouli A, Serhrouchni M, Benjelloun W. Phytotherapy of hypertension and diabetes in oriental Morocco. J Ethnopha. 1997;58(1):45-54.
[12]Mozafarrian V. Dictionary or the names of Iranian plants. Tehran: Farhang moaser publisher; 1998. [Persian]
[13]Ghahraman A. Color atlas of Iranian Flora. Tehran: Research Institute of Forests and Rangelands Publishing; 1996.
[14]Zivkovic J, Mujic I, Zekovic Z, Nikolic G, Vidovic S, Muji A. Extraction and analysis of condensed tannins in Casdanea Sativa Mill. J Cent Eur Agric. 2009;10(3):283-0.
[15]Anandh Babu PV, Sabitha KE, Shyamaladevi CS. Green tea extract impedes dyslipidaemia and development of cardiac dysfunction in streptozotocin-diabetic rats. Clin Exp Pharmacol Physiol. 2006;33(12):1184-9.
[16]Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine. 1995;2(2):137-65.
[17]Hedayati M, Pouraboli I, Pouraboli B. Effect of methanolic extract of Otostegia persica on serum levels of glucose and lipids in type I diabetic male rats. Iran J End and Met. 2010;12(4):435-58. [Persian]
[18]Alan L, Miller ND. Antioxidant flavonoids: structure, function and clinical usage. Alt Med Rev. 1996;1(2):103-11.
[19]Le K, Chiu F, Ng K. Identification and quantification of antioxidants in Fructus lycii. Food Chem. 2007;105(1):353-63.
[20]Li XM. Protective effect of Lycium barbarum polysaccharides on streptozotocin-induced oxidative stress in rats. Int J Biol Macromol. 2007;40(5):461-465.
[21]Khan S, Syed F. Bioactive Constituents from Genus Otostegia. SARJ Phy Sci. 2013;1(1):15-25.
[22]Sharififar F, Yassa F, Shafiee A. Antioxidant activity of Otostegia persica (Labiatae) and its constituents. Iran J Pharm Res. 2010;2(4):235-9. [Persian]
[23]Yamamoto Y, Oue E. Antihypertensive effect of quercetin in rats fed with a high-fat high-sucrose diet. Biosci Biotechnol Biochem. 2006;70(4):933-9.
[24]Ricardo S, Oliveira TT, Nagem TJ, Pinto AD, Oliveira MG. Effect of flavonoids morin; quercetin and nicotinic acid on lipid metabolism of rats experimentally fed with triton. Braz Arch Biol Tec. 2001;44(3):263-7.
[25]Li XM, Zhou AG. Evaluation of antioxidant activity of the poly saccharides extracted from Lycium barbarum fruits in vitro. Euro Poly J. 2007;43(2):488-97.