@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2013;19(3):129-135
ISSN: 2252-0805 The Horizon of Medical Sciences 2013;19(3):129-135
Effect of aqueous extract of Ferula assa-foetida’s resin on wound healing of streptozotocin induced diabetic rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Sadoughi S. D. (*)(*) Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Correspondence
Address:Phone: +985115013950
Fax: +985115013950
damoon.sadughi@gmail.com
Article History
Received: April 13, 2013Accepted: August 5, 2013
ePublished: October 3, 2013
ABSTRACT
Aims
The aqueous extract of Ferula assa-foetida’s resin has anti-bacterial, antiinflammatory, anti-oxidant and anti-diabetic effects. The present study was done to evaluate the effect of aqueous extract Ferula assa-foetida’s resin on wound healing in diabetic rats.
Materials & Methods In this experimental study, 18 Wistar rats were divided into the normal control (1), diabetic control (2) and diabetics treated with aqueous extract of Ferula assa-foetida’s resin groups. In all groups, 4 wounds (4mm) was created in two lateral posterial parts of body. For 2 days, 3 times a day, the experimental group was topical treatment by extract and control group was treated by normal saline. Inflammatory cells, re-epithelization and vascularization were evaluated on the 4th, 8th and 10th day.
Findings Average thickness of the epithelium on the fourth and eighth days in group 3 compared with groups 1 and 2 and on the tenth day in group 2 compared with 1 and 3 significantly increased. Density of inflammatory cells on the fourth day in groups 1 and 3 compared with group 2 and on the eighth and tenth days in group 2 compared with groups 1 and 3 significantly increased. Density of blood vessels on the fourth day in groups 1 and 3 compared with group 2 and on the eighth and tenth days in group 2 compared with groups 1 and 3 significantly increased.
Conclusion Aqueous extract of Ferula assa-foetida’s resin has a great influence on the healing of diabetic ulcers by increasing epithelial cell proliferation and blood vessel formation and accelerate the inflammatory process.
Materials & Methods In this experimental study, 18 Wistar rats were divided into the normal control (1), diabetic control (2) and diabetics treated with aqueous extract of Ferula assa-foetida’s resin groups. In all groups, 4 wounds (4mm) was created in two lateral posterial parts of body. For 2 days, 3 times a day, the experimental group was topical treatment by extract and control group was treated by normal saline. Inflammatory cells, re-epithelization and vascularization were evaluated on the 4th, 8th and 10th day.
Findings Average thickness of the epithelium on the fourth and eighth days in group 3 compared with groups 1 and 2 and on the tenth day in group 2 compared with 1 and 3 significantly increased. Density of inflammatory cells on the fourth day in groups 1 and 3 compared with group 2 and on the eighth and tenth days in group 2 compared with groups 1 and 3 significantly increased. Density of blood vessels on the fourth day in groups 1 and 3 compared with group 2 and on the eighth and tenth days in group 2 compared with groups 1 and 3 significantly increased.
Conclusion Aqueous extract of Ferula assa-foetida’s resin has a great influence on the healing of diabetic ulcers by increasing epithelial cell proliferation and blood vessel formation and accelerate the inflammatory process.
CITATION LINKS
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[2] Suba V, Murugesan T, Arunachalam G, Mandal S, Saha BP. Anti-diabetic potential of Barleria lupulina extract in rats. Phytomedicine. 2004;11(2):202-5.
[3] Benjamin A, Anthony R, Berendt H, Gunner D, John M. Diagnosis and treatment of diabetic foot infections. Med Infect. 2004;39(1):887-93.
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[9] Mallikarjuna GU, Dhanalakshmi S, Raisuddin S, Rao AR. Chemomodulatory influence of Ferula asafoetida on mammary epithelial differentiation, hepatic drug metabolizing enzymes, antioxidant profiles and N-methyl N-nitrosourea-induced mammary carcinogenesis in rats. Breast Cancer Res Treat. 2003;80(2):163-70.
[10] Iranshahy M, Iranshahi M. Traditional uses, phytochemistry and pharmacology of Ferula assa-foetida. J Ethnopharmacol. 2012;134(1):10-5.
[11]Agrawal AK, Rao CV, Sairam K, Joshi VK, Goel RK. Effect of Piper longum Linn, Zingiber officianalis Linn and Ferula species on gastric ulceration and secretion in rats. Indian J Exp Biol. 2004;38(10):994-8.
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[14] Hassani B, Saboora A, Radjabian T, Fallah Husseini H. Somatic embryogenesis of ferula assafoetida. JUST. 2008;33(4):15-23. [Persian]
[15] Khaksar S, Kesmati M, Rezaie A, Rasekh A. Topical estrogen accelerates wound healing in diabetic rats. Iran J Endocrinol Metab. 2011;12(5):544-51. [Persian]
[16] Tavakol J, Rakhshandeh H, Zamani A, Mahdavi Shahri N, Ghazizadeh L, Nourouzi M, et al. Cytotoxicity effects of Citrullus colocynthis on hep2 and l929 cell lines. Hakim J. 2005;8(2):47-54. [Persian]
[17] Anwar MM, Meki AR. Oxidative stress in streptozotocin-induced diabetic rats: Effects of garlic oil and melatonin. Comp Biochem Physiol A Mol Integr Physiol. 2004;135(4):539-47.
[18] Al-Awwadi NA, Araiz C, Bornet A. Extracts enriched in different polyphenolic families normalize increased cardiac NADPH oxidase expression while having differential effects on insulin resistance, hypertension and cardiac hypertrophy in high-fructosefed rats. J Agric Food Chem. 2005;53(1):151-7.
[19] Ebrahimian TG, Pouzoulet F, Squiban C. Cell therapy based on adipose tissue-derived stromal cells promotes physiological and pathological wound healing. Nat Med. 2009;15(2):68-79. [Persian]
[20] Bayat M, Abdi S, Javadieh F, Mohsenifar Z, Rashid MR. The effects of low-level laser therapy on bone in diabetic and nondiabetic rats. Photomed Laser Surg. 2009;27(2):703-8. [Persian]
[21] Choudhary GP. Wound healing activity of the ethanol extract of Terminalia bellirica Roxb. fruits. NPR. 2008;7(1):19-21.
[22] Qiu Z, Kwon A, Kamiyama Y. Effects of plasma fibronectin on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats. Surg Res. 2007;14(8):426-31.
[23]Sharif B, Hajhashemi V, Ghannadi A. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Ferula assafoetida. J Ethnopharmacol. 2006;89(1):67-71. [Persian]
[24] Kojima KK, Isaka P, Ondognii O. Sesquiterpenoid derivatives from ferulaeoides on wound healing in rats. Chem Pharm Bull. 2004;48(3):353-6.
[25] Xu X, Yu L, Chen G. Determination of flavonoids Ferula assafoetida L. by capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal. 2006;41(2):493-9.
[26] Tam JC, Lau KM, Liu CL, To MH, Kwok HF. The in vivo and in vitro diabetic wound healing effects of a 2-herb formula and its mechanisms of action. J Ethnopharmacol. 2011;134(3):831-8.
[27] Abdullaev FI, Frenkle GD. Effect of Ferula assa-foetida on cell colony formation and cellular nucleic acid and protein syntheses. Biofactor. 2010;18(3):201-4.
[28] Bas E, Recio MC, Abdallah M. Inhibition of the proinflammatory mediators production and anti-inflammatory effect of Ferula assafoetida. Ethnopharmacol J. 2007;110(5):414-27.
[29] - Bas E, Recio MC. New insight into the inhibition of the inflammatory response to experimental delayed-type hypersensitivity reaction in mice by Ferula assafoetida L. Eur J Pharmacol. 2007;555(6):199-210.
[30] Ilango K, Chitra V. Wound Healing, anti-oxidant activities and anti-inflammatory of glycoterpenoids from Ferula assafoetida in rats. TJPR. 2010;9(3):223-30.
[31] Diaz AM, Abadb MJ. Phenylpropanoid glycosides from Ferula assa-foetida: In vitro anti-inflammatory activity. Life Sci. 2004;74(12):2515-26.
[2] Suba V, Murugesan T, Arunachalam G, Mandal S, Saha BP. Anti-diabetic potential of Barleria lupulina extract in rats. Phytomedicine. 2004;11(2):202-5.
[3] Benjamin A, Anthony R, Berendt H, Gunner D, John M. Diagnosis and treatment of diabetic foot infections. Med Infect. 2004;39(1):887-93.
[4] Jagetia GC, Baliga MS, Aruna R, Rajanikant GK, Jain V. Augmentation of wound healing by ascorbic acid treatment in mice exposed to c-radiation. Int J Radiat Biol. 2004;80(5):347-54.
[5] Chen CF, Chen SM, Chow SY, Han PW. Wound healing activity of Ferula assa-foetida in experimental diabetes mellitus. Am J Chinese Med. 2007;9(12):205-11.
[6] Kanta J. The role of hydrogen peroxide and other reactive oxygen species in wound healing. Acta Medica. 2011;17(4):187-95.
[7] Steed DL. Clinical evaluation of recombinant human platelet-derived growth factor for the treatment of lower extremity diabetic ulcers. J Vasc Surg. 2005;21(12):71-8.
[8] Brown GL, Nanney LB, Griffen J, Cramer AB, Yancey JM, Curtsinger LJ, et al. Enhancement of wound healing by topical treatment with epidermal growth factor. N Engl J Med. 2004;321(41):76-9.
[9] Mallikarjuna GU, Dhanalakshmi S, Raisuddin S, Rao AR. Chemomodulatory influence of Ferula asafoetida on mammary epithelial differentiation, hepatic drug metabolizing enzymes, antioxidant profiles and N-methyl N-nitrosourea-induced mammary carcinogenesis in rats. Breast Cancer Res Treat. 2003;80(2):163-70.
[10] Iranshahy M, Iranshahi M. Traditional uses, phytochemistry and pharmacology of Ferula assa-foetida. J Ethnopharmacol. 2012;134(1):10-5.
[11]Agrawal AK, Rao CV, Sairam K, Joshi VK, Goel RK. Effect of Piper longum Linn, Zingiber officianalis Linn and Ferula species on gastric ulceration and secretion in rats. Indian J Exp Biol. 2004;38(10):994-8.
[12] Mahran GH. El Alfy T and Ansari S. phytochemical study of volatile oil of Afghanian asafetida. Bull Pharm Cairo Univ. 2003;12(2):101-7. [Persian]
[13] John D. One hundred useful raw drugs of the Kani tribes of Trivandrum forest division, Kerala, India. Int J Crude Drug Res. 1984;22(1):17-39.
[14] Hassani B, Saboora A, Radjabian T, Fallah Husseini H. Somatic embryogenesis of ferula assafoetida. JUST. 2008;33(4):15-23. [Persian]
[15] Khaksar S, Kesmati M, Rezaie A, Rasekh A. Topical estrogen accelerates wound healing in diabetic rats. Iran J Endocrinol Metab. 2011;12(5):544-51. [Persian]
[16] Tavakol J, Rakhshandeh H, Zamani A, Mahdavi Shahri N, Ghazizadeh L, Nourouzi M, et al. Cytotoxicity effects of Citrullus colocynthis on hep2 and l929 cell lines. Hakim J. 2005;8(2):47-54. [Persian]
[17] Anwar MM, Meki AR. Oxidative stress in streptozotocin-induced diabetic rats: Effects of garlic oil and melatonin. Comp Biochem Physiol A Mol Integr Physiol. 2004;135(4):539-47.
[18] Al-Awwadi NA, Araiz C, Bornet A. Extracts enriched in different polyphenolic families normalize increased cardiac NADPH oxidase expression while having differential effects on insulin resistance, hypertension and cardiac hypertrophy in high-fructosefed rats. J Agric Food Chem. 2005;53(1):151-7.
[19] Ebrahimian TG, Pouzoulet F, Squiban C. Cell therapy based on adipose tissue-derived stromal cells promotes physiological and pathological wound healing. Nat Med. 2009;15(2):68-79. [Persian]
[20] Bayat M, Abdi S, Javadieh F, Mohsenifar Z, Rashid MR. The effects of low-level laser therapy on bone in diabetic and nondiabetic rats. Photomed Laser Surg. 2009;27(2):703-8. [Persian]
[21] Choudhary GP. Wound healing activity of the ethanol extract of Terminalia bellirica Roxb. fruits. NPR. 2008;7(1):19-21.
[22] Qiu Z, Kwon A, Kamiyama Y. Effects of plasma fibronectin on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats. Surg Res. 2007;14(8):426-31.
[23]Sharif B, Hajhashemi V, Ghannadi A. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Ferula assafoetida. J Ethnopharmacol. 2006;89(1):67-71. [Persian]
[24] Kojima KK, Isaka P, Ondognii O. Sesquiterpenoid derivatives from ferulaeoides on wound healing in rats. Chem Pharm Bull. 2004;48(3):353-6.
[25] Xu X, Yu L, Chen G. Determination of flavonoids Ferula assafoetida L. by capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal. 2006;41(2):493-9.
[26] Tam JC, Lau KM, Liu CL, To MH, Kwok HF. The in vivo and in vitro diabetic wound healing effects of a 2-herb formula and its mechanisms of action. J Ethnopharmacol. 2011;134(3):831-8.
[27] Abdullaev FI, Frenkle GD. Effect of Ferula assa-foetida on cell colony formation and cellular nucleic acid and protein syntheses. Biofactor. 2010;18(3):201-4.
[28] Bas E, Recio MC, Abdallah M. Inhibition of the proinflammatory mediators production and anti-inflammatory effect of Ferula assafoetida. Ethnopharmacol J. 2007;110(5):414-27.
[29] - Bas E, Recio MC. New insight into the inhibition of the inflammatory response to experimental delayed-type hypersensitivity reaction in mice by Ferula assafoetida L. Eur J Pharmacol. 2007;555(6):199-210.
[30] Ilango K, Chitra V. Wound Healing, anti-oxidant activities and anti-inflammatory of glycoterpenoids from Ferula assafoetida in rats. TJPR. 2010;9(3):223-30.
[31] Diaz AM, Abadb MJ. Phenylpropanoid glycosides from Ferula assa-foetida: In vitro anti-inflammatory activity. Life Sci. 2004;74(12):2515-26.