@2024 Afarand., IRAN
ISSN: 1735-7667 Iranian Journal of Military Medicine 2010;12(1):51-56
ISSN: 1735-7667 Iranian Journal of Military Medicine 2010;12(1):51-56
Production of Clostridium botulinum Type E Antitoxin
ARTICLE INFO
Article Type
Original ResearchAuthors
Minaei M. E. ()Sa’adati M. (1)
() Biological Research Center, Basic Sciences Faculty, Imam Hossein Comprehensive University, Tehran, Iran
(1) Biological Research Center, Basic Sciences Faculty, Imam Hossein Comprehensive University, Tehran, Iran
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ebimomi@gmail.com
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ABSTRACT
Aims
The Botulinum toxin is known as the most potent toxin. In Iranian population, the type E is prevalent in individuals being poisoned by using contaminated foods (especially canned fish). So, the purpose of this study was to produce Clostridium botulinum type E antitoxin for treatment of food poisoning.
Methods For immunization of lab animals (chicken, mouse and rabbit),at first, the toxoid was injected with Freund's complete adjuvant either subcutaneously or intramuscularly and in the first to third booster injection ,it was injected with Freund's incomplete adjuvant. At the end or intervals of injections, blood sampling was done and the produced antibody’s titer was measured using the enzyme-linked immunosorbent assay or ELISA method.
Results After each booster injection, a considerable increase in serum antibody titer of lab animals was seen. The titer of Clostridium botulinum type E toxin antibodies in the serum of chicken, rabbit and mouse increased to more than 1:3200. After purification of antibody, less than 0.156 microgram of the purified mouse antibody and less than 0.312 microgram of rabbit purified antibody were able to detect the toxin.
Conclusion After the injection of toxoid, antibody increase in the body of lab animal. Production of type E specific antitoxin based on reaction between antigen and antibody is necessary for treatment of poisoning.
Methods For immunization of lab animals (chicken, mouse and rabbit),at first, the toxoid was injected with Freund's complete adjuvant either subcutaneously or intramuscularly and in the first to third booster injection ,it was injected with Freund's incomplete adjuvant. At the end or intervals of injections, blood sampling was done and the produced antibody’s titer was measured using the enzyme-linked immunosorbent assay or ELISA method.
Results After each booster injection, a considerable increase in serum antibody titer of lab animals was seen. The titer of Clostridium botulinum type E toxin antibodies in the serum of chicken, rabbit and mouse increased to more than 1:3200. After purification of antibody, less than 0.156 microgram of the purified mouse antibody and less than 0.312 microgram of rabbit purified antibody were able to detect the toxin.
Conclusion After the injection of toxoid, antibody increase in the body of lab animal. Production of type E specific antitoxin based on reaction between antigen and antibody is necessary for treatment of poisoning.
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