@2024 Afarand., IRAN
ISSN: 1735-7667 Iranian Journal of Military Medicine 2012;14(2):143-147
ISSN: 1735-7667 Iranian Journal of Military Medicine 2012;14(2):143-147
Genetic diversity of clinical strains of Salmonella enterica serovar Typhimurium
ARTICLE INFO
Article Type
Original ResearchAuthors
Ranjbar R. ()Sarshar M. (1)
() Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
(1) Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Correspondence
Address:Phone:
Fax:
ranjbar@bmsu.ac.ir
Article History
Received: October 22, 2011Accepted: March 10, 2012
ePublished:
ABSTRACT
Aims
Salmonella spp. is one of the most common pathogenic bacteria responsible for gastroenteritis in humans. Among Salmonella spp., S. typhimurium has many hosts other than humans and its prevalence rate is high in the society. The aim of this study was to investigate the ribotype diversity of S. typhimurium stains isolated in some hospitals in Tehran, Iran.
Methods In this descriptive study, clinical samples collected from different hospitals in Tehran were investigated. Bacterial isolation was carried out using selective culture media by standard procedures and identification was achieved through biochemical and serological methods. Total bacterial DNAs was extracted and ribotyping was used for molecular typing of the clinical isolates of S. typhimurium.
Results The sizes of the ribotyping bands ranged from 1.4 to 16.8 kb in all ribotypes. The S. typhimurium isolates were divided into 7 clusters based on the diversity of their ribosomal genome areas. Most isolates (5 strains) belonged to the cluster 2b. Three isolates belonged to the 6b cluster and for the rest of isolates, each belonged to one of other clusters.
Conclusion S. typhimurium strains circulating in the studied hospitals of Tehran, do not belong to a specific ribotype and have ribotype diversity, but other molecular typing methods should be implemented in order to make a more precise judjement about the presence of whether limited or widespread clones and the relationship among the different strains of this serotype.
Methods In this descriptive study, clinical samples collected from different hospitals in Tehran were investigated. Bacterial isolation was carried out using selective culture media by standard procedures and identification was achieved through biochemical and serological methods. Total bacterial DNAs was extracted and ribotyping was used for molecular typing of the clinical isolates of S. typhimurium.
Results The sizes of the ribotyping bands ranged from 1.4 to 16.8 kb in all ribotypes. The S. typhimurium isolates were divided into 7 clusters based on the diversity of their ribosomal genome areas. Most isolates (5 strains) belonged to the cluster 2b. Three isolates belonged to the 6b cluster and for the rest of isolates, each belonged to one of other clusters.
Conclusion S. typhimurium strains circulating in the studied hospitals of Tehran, do not belong to a specific ribotype and have ribotype diversity, but other molecular typing methods should be implemented in order to make a more precise judjement about the presence of whether limited or widespread clones and the relationship among the different strains of this serotype.
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