@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2013;19(1):53-57
ISSN: 2252-0805 The Horizon of Medical Sciences 2013;19(1):53-57
Effect of Canola Oil on Number and Types of White Blood Cells in Experimental Asthmatic Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Kave H. (1)Nemati A. (*)
Eftekhari H. (1)
(*) Department of Biology, Faculty of Basic Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
(1) Department of Biology, Faculty of Basic Sciences, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran
Correspondence
Address: Basic Sciences Faculty, Islamic Azad University of Mashhad, Corner of Rahnama’ei 26, Rahnama’ei Street, Mashhad, Iran.Phone: +989155046940
Fax: +985118435050
aneamati@mshdiau.ac.ir
Article History
Received: August 1, 2012Accepted: March 4, 2013
ePublished: March 10, 2013
ABSTRACT
Aims
Asthma is an airway complex disease defined by reversible airway narrowing and obstruction, airway hyper-responsiveness, airway chronic inflammation, and airway tissue remodeling. The aim of this study was to investigate the effect of canola oil on the total count and various types of leukocytes (white blood cells) in rats with experimental asthma.
Materials & Methods In this experimental research, 36 Wistar rats were divided into 4 groups: negative control, positive control, asthmatic treated by 0.5ml/kg canola oil per day and asthmatic treated by 0.7ml/kg canola oil per day.At the end of the experiment, animals were bled from the heart and the total number and percentage of white blood cells were measured and evaluated. Statistical analysis was done using SPSS 16 software and the comparison of the groups was made by ANOVA statistical test.
Findings the total count of white blood cells decreased in the experimental group 2 compared to the positive control group (p<0.05). Percentage of lymphocytes increased in experimental group 2 compared to the positive control group (p<0.01). Neutrophils percentage decreased in experimental group 2 compared to the positive control group (p<0.001). Eosinophils percentage decreased in the groups treated by canola oil compared to the positive control group (p<0.001).
Conclusion Canola oil decreases some of asthma complications including the changes in the total counts and percentage of various types of white blood cells in rats with experimental asthma.
Materials & Methods In this experimental research, 36 Wistar rats were divided into 4 groups: negative control, positive control, asthmatic treated by 0.5ml/kg canola oil per day and asthmatic treated by 0.7ml/kg canola oil per day.At the end of the experiment, animals were bled from the heart and the total number and percentage of white blood cells were measured and evaluated. Statistical analysis was done using SPSS 16 software and the comparison of the groups was made by ANOVA statistical test.
Findings the total count of white blood cells decreased in the experimental group 2 compared to the positive control group (p<0.05). Percentage of lymphocytes increased in experimental group 2 compared to the positive control group (p<0.01). Neutrophils percentage decreased in experimental group 2 compared to the positive control group (p<0.001). Eosinophils percentage decreased in the groups treated by canola oil compared to the positive control group (p<0.001).
Conclusion Canola oil decreases some of asthma complications including the changes in the total counts and percentage of various types of white blood cells in rats with experimental asthma.
CITATION LINKS
[1] Martin JG, Tamaoka M. Rat models of asthma and chronic obstructive lung disease. Pulm Pharmacol Ther. 2006;19(6):377-85.
[2] Scheerens J, van Gessel SBE, Nijkamp FP, Folkerts G. Eotaxin protein levels and airway pathology in a mouse model for allergic asthma. Eur J Pharmacol. 2002;453(1):111-7.
[3] Szelenyi I. Animal models of bronchial asthma. Inflamm Res. 2000;49(12):639-54.
[4] Halwani R, Al-Muhsen S, Hamid Q. Airway remodeling in asthma. Curr Opin Pharmacol. 2010;10(3):236-45.
[5] Neffen H, Fritscher C, Schacht FC, Levy G, Chiarella P, Soriano JB, et al. Asthma control in Latin America: The Asthma Insights and Reality in Latin America (AIRLA) survey. Rev Panam Salud Publica. 2005;17(3):191-7.
[6] Von Hertzen LC, Haahtela T. Could the risk of asthma and atopy is reduced by a vaccine that induces a strong Thelper type 1 response? Am J Respir Cell Mol Biol. 2000;22(2):139-42.
[7] Sagols E, Priymenko N. Oxidative stress in dog with heart failure: The role of dietary fatty acids and antioxidants. Vet Med Int. 2011:180206.
[8] Charavaryamath C, Janardhan KS, Townsend HG, Willson P, Singh B. Multiple exposures to swine barn air induce lung inflammation and airway hyper-responsiveness. Respir Res. 2005;6:50.
[9] Murdoch JR, Lloyd CM. Chronic inflammation and asthma. Mutat Res. 2010;690(1-2):24-39.
[10] Park HS, Kim SR, Kim JO, Lee YC. The roles of phytochemicals in bronchial asthma. Molecules. 2010;15(10):6810-34.
[11] Sporik R, Holgate ST, Platts-Mills TA, Cogswell JJ. Exposure to house-dust mite allergen (Der p I) and the development of asthma in childhood: A prospective study. N Engl J Med. 1990;323(8):502-7.
[12]Boskabady MH, Klahdoz GH. Prevalence of asthma symptoms among the adult population in the city of Mashhad. Respirology. 2002;7(3):267-72.
[13] Global Initiative for Asthma. Global strategy for asthma management and prevention, WHO/ NHLBI workshop report. Washington: National Heart-Lung and Blood Institute Publication; 1995.
[14] Dinwidde R. The diagnosis and management living of pediatric respiratory disease. London: Churchill Stone Publisher; 1990.
[15] Kabiri Rad M, Neamati A, Boskabady MH, Mahdavi- Shahri N. The preventive effect of Brassica napus L. oil on pathophysiological changes of respiratory system in experimental asthmatic rat. Avicenna J Phytomed. 2013;3(1):56-63.
[16] Padrid P, Snook S, Shiue P, Solway J, Leff AR. Persistent airway hyper responsiveness and histological alteration after chronic antigen challenge in cats. Am J Respir Crit Care Med. 1995;151(1):184-93.
[17] Nash AA, Powers HJ, Primhak RA. Antioxidant status in asthma. Pediatr Pulmonol. 1994;18:34-8.
[18]Ard C, Stenton SC, Bird G, Hendrick DJ, Walters EH. Number and activity of inflammatory cells in bronchoalveolar lavage fluid in asthma and their relation to airway responsiveness. Thorax. 1998;43(9):684-92.
[19] Cluzel M, Damon M, Chanez P. Enhanced alveolar cell luminal-dependent chemiluminescence in asthma. J Allergy Clin Immunol. 1987;80(2):195-201.
[20] Greene LS. Asthma and oxidant stress: Nutritional, environmental and genetic risk factors. J Am Coll Nutr. 1995;14(4):317-24.
[21]Cohn L, Elias JA, Chupp GL. Asthma: Mechanisms of disease persistence and progression. Annu Rev Immunol. 2004;22:789-815.
[22] Randolph DA, Stephens R, Carruthers CJ. Cooperation between Th1 and Th2 cells in a murine model of eosinophilic airway inflammation. J Clin Invest. 1999;104(8):1021-9.
[23] Oh SR, Lee MY, Ahn K, Kwon OK, Lee J, Lee S, et al. Suppressive effect of verproside isolated from Pseudolysimachion longifolium on airway inflammation in a mouse model of allergic asthma. Int Immunopharmacol. 2006;6(6):978-86.
[24]Mosmann TR, Coffman RL. Th1 and Th2 cells: Different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol. 1989;7:145-73.
[25] Hirota JA, Nguyen TTB, Schaafsma D, Sharma P, Tran T. Airway smooth muscle in asthma: Phenotype plasticity and function. Pulm Pharmacol Ther. 2009;22(5):370-8.
[26]Pinzone HA, Carlson BW, Kotses H, Creer TL. Prediction of asthma episodes in children using peak expiratory flow rates, medication compliance and exercise data. Ann Allergy. 1991;67(5):481-6.
[27]Romagnani S. Lymphokine production by human T cells in disease states. Annu Rev Immunol. 1994;12:227-57.
[28] Boskabady MH, Snashall PD. Bronchial responsiveness to beta-adrenergic stimulation and enhanced beta-blockade in asthma. Respirology. 2000;5(2):111-8.
[29] Paula Portela C, Oliveira Massoco C, Lima WT, Palermo-Neto J. Stress-induced increment on total bronchoalveolar cell count in OVA-sensitized rats. Physiol Behav. 2001;72(3):415-20.
[30]Boskabady MH, Kiani S. The effect of exposure of guinea pigs to cigarette smoke and their sensitization with ovalbumin in tracheal responsiveness to histamine and histamine (H1) receptor blockade by chlorpheniramine. Pathophysiology. 2007;14(2):97-104.
[2] Scheerens J, van Gessel SBE, Nijkamp FP, Folkerts G. Eotaxin protein levels and airway pathology in a mouse model for allergic asthma. Eur J Pharmacol. 2002;453(1):111-7.
[3] Szelenyi I. Animal models of bronchial asthma. Inflamm Res. 2000;49(12):639-54.
[4] Halwani R, Al-Muhsen S, Hamid Q. Airway remodeling in asthma. Curr Opin Pharmacol. 2010;10(3):236-45.
[5] Neffen H, Fritscher C, Schacht FC, Levy G, Chiarella P, Soriano JB, et al. Asthma control in Latin America: The Asthma Insights and Reality in Latin America (AIRLA) survey. Rev Panam Salud Publica. 2005;17(3):191-7.
[6] Von Hertzen LC, Haahtela T. Could the risk of asthma and atopy is reduced by a vaccine that induces a strong Thelper type 1 response? Am J Respir Cell Mol Biol. 2000;22(2):139-42.
[7] Sagols E, Priymenko N. Oxidative stress in dog with heart failure: The role of dietary fatty acids and antioxidants. Vet Med Int. 2011:180206.
[8] Charavaryamath C, Janardhan KS, Townsend HG, Willson P, Singh B. Multiple exposures to swine barn air induce lung inflammation and airway hyper-responsiveness. Respir Res. 2005;6:50.
[9] Murdoch JR, Lloyd CM. Chronic inflammation and asthma. Mutat Res. 2010;690(1-2):24-39.
[10] Park HS, Kim SR, Kim JO, Lee YC. The roles of phytochemicals in bronchial asthma. Molecules. 2010;15(10):6810-34.
[11] Sporik R, Holgate ST, Platts-Mills TA, Cogswell JJ. Exposure to house-dust mite allergen (Der p I) and the development of asthma in childhood: A prospective study. N Engl J Med. 1990;323(8):502-7.
[12]Boskabady MH, Klahdoz GH. Prevalence of asthma symptoms among the adult population in the city of Mashhad. Respirology. 2002;7(3):267-72.
[13] Global Initiative for Asthma. Global strategy for asthma management and prevention, WHO/ NHLBI workshop report. Washington: National Heart-Lung and Blood Institute Publication; 1995.
[14] Dinwidde R. The diagnosis and management living of pediatric respiratory disease. London: Churchill Stone Publisher; 1990.
[15] Kabiri Rad M, Neamati A, Boskabady MH, Mahdavi- Shahri N. The preventive effect of Brassica napus L. oil on pathophysiological changes of respiratory system in experimental asthmatic rat. Avicenna J Phytomed. 2013;3(1):56-63.
[16] Padrid P, Snook S, Shiue P, Solway J, Leff AR. Persistent airway hyper responsiveness and histological alteration after chronic antigen challenge in cats. Am J Respir Crit Care Med. 1995;151(1):184-93.
[17] Nash AA, Powers HJ, Primhak RA. Antioxidant status in asthma. Pediatr Pulmonol. 1994;18:34-8.
[18]Ard C, Stenton SC, Bird G, Hendrick DJ, Walters EH. Number and activity of inflammatory cells in bronchoalveolar lavage fluid in asthma and their relation to airway responsiveness. Thorax. 1998;43(9):684-92.
[19] Cluzel M, Damon M, Chanez P. Enhanced alveolar cell luminal-dependent chemiluminescence in asthma. J Allergy Clin Immunol. 1987;80(2):195-201.
[20] Greene LS. Asthma and oxidant stress: Nutritional, environmental and genetic risk factors. J Am Coll Nutr. 1995;14(4):317-24.
[21]Cohn L, Elias JA, Chupp GL. Asthma: Mechanisms of disease persistence and progression. Annu Rev Immunol. 2004;22:789-815.
[22] Randolph DA, Stephens R, Carruthers CJ. Cooperation between Th1 and Th2 cells in a murine model of eosinophilic airway inflammation. J Clin Invest. 1999;104(8):1021-9.
[23] Oh SR, Lee MY, Ahn K, Kwon OK, Lee J, Lee S, et al. Suppressive effect of verproside isolated from Pseudolysimachion longifolium on airway inflammation in a mouse model of allergic asthma. Int Immunopharmacol. 2006;6(6):978-86.
[24]Mosmann TR, Coffman RL. Th1 and Th2 cells: Different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol. 1989;7:145-73.
[25] Hirota JA, Nguyen TTB, Schaafsma D, Sharma P, Tran T. Airway smooth muscle in asthma: Phenotype plasticity and function. Pulm Pharmacol Ther. 2009;22(5):370-8.
[26]Pinzone HA, Carlson BW, Kotses H, Creer TL. Prediction of asthma episodes in children using peak expiratory flow rates, medication compliance and exercise data. Ann Allergy. 1991;67(5):481-6.
[27]Romagnani S. Lymphokine production by human T cells in disease states. Annu Rev Immunol. 1994;12:227-57.
[28] Boskabady MH, Snashall PD. Bronchial responsiveness to beta-adrenergic stimulation and enhanced beta-blockade in asthma. Respirology. 2000;5(2):111-8.
[29] Paula Portela C, Oliveira Massoco C, Lima WT, Palermo-Neto J. Stress-induced increment on total bronchoalveolar cell count in OVA-sensitized rats. Physiol Behav. 2001;72(3):415-20.
[30]Boskabady MH, Kiani S. The effect of exposure of guinea pigs to cigarette smoke and their sensitization with ovalbumin in tracheal responsiveness to histamine and histamine (H1) receptor blockade by chlorpheniramine. Pathophysiology. 2007;14(2):97-104.