@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2013;19(1):41-45
ISSN: 2252-0805 The Horizon of Medical Sciences 2013;19(1):41-45
Effect of Anethum graveolens Hydroalcoholic Extract on Pentylenetetrazole-induced Paroxysm
ARTICLE INFO
Article Type
Original ResearchAuthors
Roohbakhsh A. (*)Esmaeili H. (1)
Asami Z. (1)
Arab Baniasad F. (1)
Shamsizadeh A. (1)
Rezvani M. (2)
(*) Physiology & Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
(1) Physiology & Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
(2) Department of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Correspondence
Article History
Received: March 16, 2012Accepted: March 4, 2013
ePublished: March 10, 2013
ABSTRACT
Aims
Epilepsy is one of the common neurological disorders. Today, there are many drugs for treatment of this disease, which most of these chemical drugs have harmful effects on patients’ health. The aim of the present study was to investigate the effect of hydroalcoholic extract of dill (Anethum graveolens) seeds on the pentylenetetrazole-induced chemical kindling.
Materials & Methods This experimental study was conducted on 50balb/c male mice weighting 25-35g.Mice were divided into five groups of 10. Three experimental groups received hydroalcoholic extract of dill seeds by three concentrations of 150, 300 and 600mg/kg of the animals’ body weight. Positive control group received diazepam (3mg/kg) instead of the extract. Likewise,negative control groups received sterile normal saline (6mg/kg) instead of the extract. In order to exactly compare the different concentrations of the drug and extract on the times after injection on the paroxysm values, ANOVA and supplementary Tukey test as well as SPSS 18 software were used.
Findings At doses of 150 and 300mg/kg, dill had desirable inhibitory effect on the epileptic behavior of animals at different days (p<0.01). Moreover, at dose of 600mg/kg, it favorably inhibited the mice’ epileptic movements (p<0.01).
Conclusion The hydroalcoholic extract of dill seeds has antiepileptic (anti-seizure) effect at dose of 600mg/kg on mice.
Materials & Methods This experimental study was conducted on 50balb/c male mice weighting 25-35g.Mice were divided into five groups of 10. Three experimental groups received hydroalcoholic extract of dill seeds by three concentrations of 150, 300 and 600mg/kg of the animals’ body weight. Positive control group received diazepam (3mg/kg) instead of the extract. Likewise,negative control groups received sterile normal saline (6mg/kg) instead of the extract. In order to exactly compare the different concentrations of the drug and extract on the times after injection on the paroxysm values, ANOVA and supplementary Tukey test as well as SPSS 18 software were used.
Findings At doses of 150 and 300mg/kg, dill had desirable inhibitory effect on the epileptic behavior of animals at different days (p<0.01). Moreover, at dose of 600mg/kg, it favorably inhibited the mice’ epileptic movements (p<0.01).
Conclusion The hydroalcoholic extract of dill seeds has antiepileptic (anti-seizure) effect at dose of 600mg/kg on mice.
CITATION LINKS
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[14] Palizvan MR, Ghaznavi Rad E, Amini Komijani A. The effect of verapamil and naloxone on seizure attacks induced by chemical kindling of the rats. Zanjan Univ Med Sci J. 2004;85:1-7. [Persian]
[15]Nazifi M, Fathi Azarbayjani F, Ilkhanipur M, FarokhiIranian F. Modulatory effect of aspirin on anxietylike behavior induced by pentylenetetrazole kindling. J Neurol. 2009;8(25):427-35. [Persian]
[16]Bienvenu E, Amabeoku GJ, Eagles PK, Scott G, Springfield EP. Anticonvulsant activity of aqueous extract of Leonotis leonurus. Phytomedicine. 2002;9(3):217-23.
[17] Kupferberg H. Animal models used in the screening of antiepileptic drugs. Epilepsia. 2001;42(4):7-12
[18] Brunton LL, Chabner BA, Knollmann BC. Goodman and Gilman's: The pharmacological basis of therapeutics. 12th ed. China: McGraw-Hill Company; 2011.
[19] Orhan I, Kartal M, Kan Y, Sener B. Activity of essential oils and individual components against acetyl- and butyrylcholinesterase. Z Naturforsch C. 2008;63(7-8):547- 53.
[20] Getova DP, Dimitrova DS. Effects of the anticholinesterase drug tacrine on the development of PTZ kindling and on learning and memory processes in mice. Folia Med. 2000;42(4):5-9.
[2] Kaur GJ, Arora DS. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complement Altern Med. 2009;9:30.
[3] Zargari A. Medicinal plants. Tehran: Tehran University Publications; 1991. [Persian]
[4] Jana S, Shekhawat GS. Anethum graveolens: An Indian traditional medicinal herb and spice. Pharmacogn Rev. 2010;4(8):179-84.
[5] Gharib Naseri MK, Heidari A. Antispasmodic effect of Anethum graveolens fruit extract on rat ileum. Int J Pharmacol. 2007;3:260-4.
[6] Hajhashemi V, Abbasi N. Hypolipidemic activity of Anethum graveolens in rats. Phytother Res. 2008;22(3):372- 5.
[7]Panda S. The effect of Anethum graveolens L. (dill) on corticosteroid induced diabetes mellitus: Involvement of thyroid hormones. Phytother Res. 2008;22(12):1695-7.
[8] Hosseinzadeh H, Karimi GR, Ameri M. Effects of Anethum graveolens L. seed extracts on experimental gastric irritation models in mice. BMC Pharmacol. 2002;19:21.
[9] Yung-Shin S, Jau-Tien L, Yuan-Tsung C, Chia-Jung C, Deng-Jye Y. Evaluation of antioxidant ability of ethanolic extract from dill (Anethum graveolens L.) flower. Food Chem. 2009;115:515-21.
[10] Papp AFO, Erdelyi L. The ionic mechanism of the pentylenetetrazol convulsions. Acta Biol Hung. 1987;38(3- 4):349-61.
[11] Hansen SL, Sperling BB, Sanchez C. Anticonvulsant and antiepileptogenic effects of GABAA receptor ligands in pentylenetetrazole-kindled mice. Prog Neuropsychopharmacol Biol Psychiatry. 2004;28(1):105-13.
[12] Krug M, Koch M, Grecksch G, Schulzeck K. Pentylentetrazol kindling changes the ability to induced potentiation phenomena in the hipocampal CA1 region. Physiol Behav. 1997;62(4):721-7.
[13] Shafaei A, Fereidoni M, Moghimi A, Behnamrasooli M. Effect of ultra low dose morphine on seizure induced by pentylentetrazol. Physiol Pharmacol. 2009;12(4):307-13. [Persian]
[14] Palizvan MR, Ghaznavi Rad E, Amini Komijani A. The effect of verapamil and naloxone on seizure attacks induced by chemical kindling of the rats. Zanjan Univ Med Sci J. 2004;85:1-7. [Persian]
[15]Nazifi M, Fathi Azarbayjani F, Ilkhanipur M, FarokhiIranian F. Modulatory effect of aspirin on anxietylike behavior induced by pentylenetetrazole kindling. J Neurol. 2009;8(25):427-35. [Persian]
[16]Bienvenu E, Amabeoku GJ, Eagles PK, Scott G, Springfield EP. Anticonvulsant activity of aqueous extract of Leonotis leonurus. Phytomedicine. 2002;9(3):217-23.
[17] Kupferberg H. Animal models used in the screening of antiepileptic drugs. Epilepsia. 2001;42(4):7-12
[18] Brunton LL, Chabner BA, Knollmann BC. Goodman and Gilman's: The pharmacological basis of therapeutics. 12th ed. China: McGraw-Hill Company; 2011.
[19] Orhan I, Kartal M, Kan Y, Sener B. Activity of essential oils and individual components against acetyl- and butyrylcholinesterase. Z Naturforsch C. 2008;63(7-8):547- 53.
[20] Getova DP, Dimitrova DS. Effects of the anticholinesterase drug tacrine on the development of PTZ kindling and on learning and memory processes in mice. Folia Med. 2000;42(4):5-9.