@2024 Afarand., IRAN
ISSN: 1735-7675 Kowsar Medical Journal 2011;15(4):197-202
ISSN: 1735-7675 Kowsar Medical Journal 2011;15(4):197-202
Production of chicken egg yolk immunoglobulin against Helicobacter pylori UreC protein by DNA vaccination
ARTICLE INFO
Article Type
Original ResearchAuthors
Amiri Javid Sh. (1 )Mousavi S. L. (* )
Salmanian A. H. (2 )
Basiri M. (3 )
(* ) Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran
(1 ) Department of Biology, Khatam University, Tehran, Iran
(2 ) Plant Biotechnology Research Center, National Institute of Genetic Engineering & Biotechnology, Tehran, Iran
(3 ) Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran
Correspondence
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slmousavi@shahed.ac.ir
Article History
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ABSTRACT
Aims
Current therapies against Helicobacter pylori infection, which is associated with gastritis, peptic ulcer and gastric cancer, are performed by antibiotic use. Due to increase of antibiotic resistance and difficulty and cost of treatment, new approaches have focused on using specific antibodies. Accordingly, efficiency of antibodies specific to H. pylori urease has been demonstrated. Considering the longtime benefit and efficacy of DNA vaccination, this study was designed on order to use DNA vaccination for generation of egg yolk immunoglobulin against UreC subunit of H. pylori urease.
Materials & Methods H. pylori ureC gene was cloned into eukaryotic pCI expression vector. Recombinant pCI-ureC plasmid, amplified in E. coli host were purified and used for genetic immunization of chickens. IgY recovered from egg yolk, using Poly Ethylene Glycol precipitation and analyzed by indirect ELISA and urease test
Results Using DNA vaccination, specific and biologically active IgY antibodies were produced and extracted, which based on ELISA results is able to detect UreC subunit of urease.
Conclusion DNA immunization can be used as a productive and economic method to generate efficient anti-urease antibodies for therapeutic proposes.
Materials & Methods H. pylori ureC gene was cloned into eukaryotic pCI expression vector. Recombinant pCI-ureC plasmid, amplified in E. coli host were purified and used for genetic immunization of chickens. IgY recovered from egg yolk, using Poly Ethylene Glycol precipitation and analyzed by indirect ELISA and urease test
Results Using DNA vaccination, specific and biologically active IgY antibodies were produced and extracted, which based on ELISA results is able to detect UreC subunit of urease.
Conclusion DNA immunization can be used as a productive and economic method to generate efficient anti-urease antibodies for therapeutic proposes.
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