@2024 Afarand., IRAN
ISSN: 1735-7675 Kowsar Medical Journal 2010;15(2):89-93
ISSN: 1735-7675 Kowsar Medical Journal 2010;15(2):89-93
Measuring the amount of M1 Aflatoxin in pasteurized milks
ARTICLE INFO
Article Type
Original ResearchAuthors
Riazipour M. (1 )Tavakkoli H. R. (*)
Razzaghi Abyane M. (2 )
Raf’ati H. (3 )
Sadr Momtaz S. M. (4 )
(*) Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
(1 ) Therapeutic Microbial Toxins Research Center, Baqiyatallah Institute of Medical Sciences, Tehran, Iran
(2 ) Department of Mycology, Pasteur Institute, Tehran, Iran
(3 ) Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
(4 ) Faculty of Health, Baqiyatallah University of Medical Sciences, Tehran, Iran
Correspondence
Address:Phone:
Fax:
h.tavakoli1344@yahoo.com
Article History
Received:Accepted:
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ABSTRACT
Aims
This study was conducted to investigate the pasteurized milks contamination with M1 Aflatoxin (AFM1) and comparison of the products of two main factories that provide one of Tehran Universities dairy needs, in terms of contaminating with this mycotoxin.
Materials & Methods Fifty milk samples produced by two dairy factories were randomly selected during two cold (winter 2008) and warm (summer 2009) seasons and their AFM1 concentration was determined by a competitive Enzyme-Linked Immuno Sorbent Assay (ELISA) method. The mean differences were analyzed using student T-test in software INSTATA environment.
Results 84% of the examined milk samples were contaminated with AFM1 by measurable amounts. Mean of the mycotoxin concentration in whole samples was 20.7±14.6 ng/l ranging from 0 to 63.4 ng/l. AFM1 contamination was higher than Iran national standard (50 ng/ l) only in two (4%) of the milk samples. The mean concentration of AFM1 was higher in no. 1 company’s products (25.3±15.7) in comparison with no.2 company (15.4±11.6; p<0.01). The no. 1 company’s winter products (31.2±15 ng/l) were more contaminated in contrasting with its summer products (18.5±14.2 ng/l; p<0.05).
Conclusion High prevalence of AFM1 contamination in pasteurized milk samples is worrying and notifies the necessity of preventing measures to reduce entrance of B1 Aflatoxin to dairy animals’ feed and more controlling measures on milk distribution.
Materials & Methods Fifty milk samples produced by two dairy factories were randomly selected during two cold (winter 2008) and warm (summer 2009) seasons and their AFM1 concentration was determined by a competitive Enzyme-Linked Immuno Sorbent Assay (ELISA) method. The mean differences were analyzed using student T-test in software INSTATA environment.
Results 84% of the examined milk samples were contaminated with AFM1 by measurable amounts. Mean of the mycotoxin concentration in whole samples was 20.7±14.6 ng/l ranging from 0 to 63.4 ng/l. AFM1 contamination was higher than Iran national standard (50 ng/ l) only in two (4%) of the milk samples. The mean concentration of AFM1 was higher in no. 1 company’s products (25.3±15.7) in comparison with no.2 company (15.4±11.6; p<0.01). The no. 1 company’s winter products (31.2±15 ng/l) were more contaminated in contrasting with its summer products (18.5±14.2 ng/l; p<0.05).
Conclusion High prevalence of AFM1 contamination in pasteurized milk samples is worrying and notifies the necessity of preventing measures to reduce entrance of B1 Aflatoxin to dairy animals’ feed and more controlling measures on milk distribution.
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