@2024 Afarand., IRAN
ISSN: 1735-7675 Kowsar Medical Journal 2010;15(1):11-15
ISSN: 1735-7675 Kowsar Medical Journal 2010;15(1):11-15
Effect of oral morphine consumption on hippocampus development in Wistar rats embryo
ARTICLE INFO
Article Type
Original ResearchAuthors
Ramazani M. (1)Hamidi E. (2)
Hajizadeh Moghadam A. (3)
Bahadoran H. (4)
Sahraei H. ()
() “Department of Physiology and Biophysics, Faculty of Medicine” and “Applied Neuroscience Research Center”, Baqiyatallah University of Medical Sciences, Tehran, Iran
(1) Department of Biology, School of Basic Sciences, Ashtian Branch, Islamic Azad University,, Ashtian, Iran
(2) Department of Biology, School of Basic Sciences, Payam-e-Noor University, Tehran, Iran
(3) Department of Biology, School of Basic Sciences, Babolsar University, Babolsar, Iran
(4) “Department of Anatomy, Faculty of Medicine” and “Behavioral Sciences Research Center” , Baqiyatallah University of Medical Sciences, Tehran, Iran
Correspondence
Address:Phone:
Fax:
h.sahraei@bmsu.ac.ir
Article History
Received: February 13, 2008Accepted: September 10, 2008
ePublished:
ABSTRACT
Aims
In the present study, the effect of oral morphine consumption on hippocampus development in Wistar rat embryos of weight range of 250-300g was investigated.
Materials & Methods 12 pregnant Wistar rats with weight range of 250-300g were fed daily with 0.05 g morphine solution in water until day 19 of pregnancy. Then, pregnant rats went under surgery and the embryo was removed with the uterus and was fixed in formalin 10% for 60 days. Then, using some stages such as processing, cutting and staining, the samples were provided and evaluated using light microscope and MOTIC software.
Results Diameter of inner, middle and an outer layer of hippocampus reduced in the experimental group comparing to control group; while, the cell number increased in experimental group comparing to control group but their cell size was considerably reduced.
Conclusion Oral morphine consumption may delay hippocampus development in Wistar rat embryos, which it is seen in cell increment of this area to its different layers and layer diameter decrement of each layer.
Materials & Methods 12 pregnant Wistar rats with weight range of 250-300g were fed daily with 0.05 g morphine solution in water until day 19 of pregnancy. Then, pregnant rats went under surgery and the embryo was removed with the uterus and was fixed in formalin 10% for 60 days. Then, using some stages such as processing, cutting and staining, the samples were provided and evaluated using light microscope and MOTIC software.
Results Diameter of inner, middle and an outer layer of hippocampus reduced in the experimental group comparing to control group; while, the cell number increased in experimental group comparing to control group but their cell size was considerably reduced.
Conclusion Oral morphine consumption may delay hippocampus development in Wistar rat embryos, which it is seen in cell increment of this area to its different layers and layer diameter decrement of each layer.
CITATION LINKS