@2024 Afarand., IRAN
ISSN: 1735-7667 Iranian Journal of Military Medicine 2011;12(4):203-209
ISSN: 1735-7667 Iranian Journal of Military Medicine 2011;12(4):203-209
Effect of sulfur mustard on the epithelial cell necrosis of urinary duct of kidney in rat
ARTICLE INFO
Article Type
Original ResearchAuthors
Mirshafiee Gh. A. (* )Sadraei S. H. (1 )
Bahadoran H. (1 )
Kaka Gh. R. (2 )
Dashtnavard H. (1 )
Nourani M. R. (3 )
Mofid M. (1 )
Mahdavinasab H. (1 )
Imani Fouladi A. A. (4 )
(* ) Department of Anatomy, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
(1 ) Department of Anatomy, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
(2 ) Neuroscience Research Center, Baqiyatallah Institute of Medical Sciences, Tehran, Iran
(3 ) Chemical Injuries Research Center, Baqiyatallah Institute of Medical Sciences, Tehran, Iran
(4 ) Molecular Cell Biology Research Center, Baqiyatallah Institute of Medical Sciences, Tehran, Iran
Correspondence
Article History
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ABSTRACT
Aims
Kidney is a high vascularized organ which can be affected by sulfur mustard. Sulfur mustard causes tubular necrosis and urinary epithelial cell sloughing. The aim of this study was to evaluate the cellular and tissue damages in rats exposed to sulfur mustard.
Methods This experimental study was performed on 100 rats. 10 mg/kg was assigned as the desirable dose which rats could live at least 10 weeks while receiving it. 2.5 and 5 mg/kg were also assigned as doses in which tissue changes were studied. Tissue changes were evaluated after 2, 14, 28 and 56 days in 12 experimental groups (3 groups for each time period each one receiving one of the 3 mentioned doses). 4 natural groups (one for each time period) received no injection and 4 control groups only received Tyrode’s solution. Prepared slides of all groups were compared and histological changes were evaluated. Data was analyzed using one-way ANOVA and Tukey’s Post-hoc test by SPSS 13 software.
Results Data of this study revealed no remarkable difference between natural and control groups. Tubular necrosis and urinary epithelial cell sloughing were observed in all experimental groups. The rate was statistically significant only in group which received 10 mg/kg sulfur mustard for 56 days compared to the control group.
Conclusion Exposure to sulfur mustard cause tubular necrosis and urinary epithelial cell sloughing in rats and its increase in statistically significant in rats receiving 10 mg/kg for 8 weeks.
Methods This experimental study was performed on 100 rats. 10 mg/kg was assigned as the desirable dose which rats could live at least 10 weeks while receiving it. 2.5 and 5 mg/kg were also assigned as doses in which tissue changes were studied. Tissue changes were evaluated after 2, 14, 28 and 56 days in 12 experimental groups (3 groups for each time period each one receiving one of the 3 mentioned doses). 4 natural groups (one for each time period) received no injection and 4 control groups only received Tyrode’s solution. Prepared slides of all groups were compared and histological changes were evaluated. Data was analyzed using one-way ANOVA and Tukey’s Post-hoc test by SPSS 13 software.
Results Data of this study revealed no remarkable difference between natural and control groups. Tubular necrosis and urinary epithelial cell sloughing were observed in all experimental groups. The rate was statistically significant only in group which received 10 mg/kg sulfur mustard for 56 days compared to the control group.
Conclusion Exposure to sulfur mustard cause tubular necrosis and urinary epithelial cell sloughing in rats and its increase in statistically significant in rats receiving 10 mg/kg for 8 weeks.
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