@2024 Afarand., IRAN
ISSN: 1735-7675 Kowsar Medical Journal 2010;15(2):95-98
ISSN: 1735-7675 Kowsar Medical Journal 2010;15(2):95-98
Sensitivity and specificity of ELISA test in diagnosis of brucellosis
ARTICLE INFO
Article Type
Original ResearchAuthors
Vakili Z. (1 )Momen Heravi M. (* )
Sharif A. R. (2 )
Masoomi M. (2 )
(* ) Department of Infectious Diseases, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
(1 ) Department of Pathology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
(2 ) Department of Infectious Diseases, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
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mansoreheravi@yahoo.com
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ABSTRACT
Aims
Brucellosis is a common disease with different clinical forms and diagnostic methods. Regarding to the increasing use of ELISA test as a new diagnostic method and differences between the results of standard ELISA and agglutination tests, this study was conducted to determine the sensitivity and specificity of ELISA method for diagnosis of brucellosis.
Materials & Methods This cross-sectional study was done in Kashan, Iran in 2007, on 457 blood samples of persons with clinically suspected brucellosis who were referred to laboratory by infectious disease’ specialist and on 50 individuals without history of brucellosis as the control group. The wright, coombs wright and 2ME tests as the standard tests and ELISA IgG and IgM were done and characteristic and sensitivity of ELISA was compared with standard tests and the results were presented as the descriptive statistics.
Results The sensitivity of IgG and IgM ELISA tests was 93.7% and 12.5%, respectively. IgG and IgM ELISA test’s specificity was 70.6% and 100%, the positive predictive value of IgG and IgM ELISA tests was 19.4% and 100%, respectively and the negative predictive value of ELISA IgG and IgM was 99.3% and 94%.
Conclusion Regarding the high sensitivity of IgG ELISA and high specificity of IgM ELISA, the ELISA test can be used along with agglutination tests for diagnosis of brucellosis.
Materials & Methods This cross-sectional study was done in Kashan, Iran in 2007, on 457 blood samples of persons with clinically suspected brucellosis who were referred to laboratory by infectious disease’ specialist and on 50 individuals without history of brucellosis as the control group. The wright, coombs wright and 2ME tests as the standard tests and ELISA IgG and IgM were done and characteristic and sensitivity of ELISA was compared with standard tests and the results were presented as the descriptive statistics.
Results The sensitivity of IgG and IgM ELISA tests was 93.7% and 12.5%, respectively. IgG and IgM ELISA test’s specificity was 70.6% and 100%, the positive predictive value of IgG and IgM ELISA tests was 19.4% and 100%, respectively and the negative predictive value of ELISA IgG and IgM was 99.3% and 94%.
Conclusion Regarding the high sensitivity of IgG ELISA and high specificity of IgM ELISA, the ELISA test can be used along with agglutination tests for diagnosis of brucellosis.
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