@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(3):169-174
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(3):169-174
Effect of Hydroalcoholic Extract of Kelussia odoratissima Mozaffarian on Uterus Contractions of Mature Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Jafarzadeh L. (1)Habibian R. (2)
Rafieian Kopaei M. (*)
Mohammadzadeh Z. (3)
(*) Medical Plants Research Center, Shahrekord University of Medical Sciences, Sharekord, Iran
(1) Obstetrics and Gynecology Department, Shahrekord University of Medical Science, Shahrekord, Iran
(2) Infectious Disease Department, Medicine Faculty, Shahrekord University of Medical Science, Shahrekord, Iran
(3) Medical Sciences Department, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
Correspondence
Address: Medical Plants Research Center, Shahrekord University of Medical Sciences, Rahmatiyeh, Shahrekord, IranPhone: +983833346692
Fax: +983833349506
rafieian@yahoo.com
Article History
Received: January 5, 2015Accepted: July 7, 2015
ePublished: September 20, 2015
ABSTRACT
Aims
Kelussia odoratissima is a plant from Umbelliferae family that has nutritional and pharmacological properties. The antispasmodic effect of this plant has been mentioned in Iranian traditional medicine. The aim of study was to assess the effect of alcoholic extract of Kelussia odoratissima on uterus contractions of adult rats.
Materials & Methods In this experimental study, 16 adult female Wistar rats were studied and were randomly divided into two groups (each consisted of 8 rats); receiver of Tyrod solution (first group) and receiver of Dejalon solution (second group) groups. A 10-15mm sample of uterus smooth muscle were placed in an organ bath containing Tyrod or Dejalon solution (10ml), and contractions were recorded with Isometric method after inducing with KCl and Oxytocin. The 0.125, 0.25 and 0.5mg/ml final concentrations of the alcoholic extract of Kelussia odoratissima was added to the organ bath. Data were statistically analyzed by SPSS 17 software using repeated measures ANOVA.
Findings The alcoholic extract of Kelussia odoratissima significantly decreased the KCl-induced (p<0.001) and Oxytocin-induced (p<0.002) contractions in a concentration dependent manner.
Conclusion The alcoholic extract of Kelussia odoratissima inhibits the contractions of adult rat uterus in a concentration dependent manner.
Materials & Methods In this experimental study, 16 adult female Wistar rats were studied and were randomly divided into two groups (each consisted of 8 rats); receiver of Tyrod solution (first group) and receiver of Dejalon solution (second group) groups. A 10-15mm sample of uterus smooth muscle were placed in an organ bath containing Tyrod or Dejalon solution (10ml), and contractions were recorded with Isometric method after inducing with KCl and Oxytocin. The 0.125, 0.25 and 0.5mg/ml final concentrations of the alcoholic extract of Kelussia odoratissima was added to the organ bath. Data were statistically analyzed by SPSS 17 software using repeated measures ANOVA.
Findings The alcoholic extract of Kelussia odoratissima significantly decreased the KCl-induced (p<0.001) and Oxytocin-induced (p<0.002) contractions in a concentration dependent manner.
Conclusion The alcoholic extract of Kelussia odoratissima inhibits the contractions of adult rat uterus in a concentration dependent manner.
CITATION LINKS
[1]French L. Dysmenorrhea in adolescents: Diagnosis and treatment . Pediatr Drugs. 2008;10(1):1-7.
[2]Serashti M, Azari P, Rafiean M, Kheiri S. Use of herbal medicines by pregnant women in Shahr-e-Kord. J Reprod Infertility. 2006;7(2):125-31.
[3]Omidbaigi R, Sefidkon F, Saeedi K. Essential oil content and composition of Kelussia odoratissima Mozaff as an Iranian endemic plant. J Essent Oil Bearing Plants. 2008;11(6): 594-7.
[4]Salimi M, Ebrahimi A, Shojaee Asadieh Z, Saei Dehkordi SS. Essential oil composition of Kelussia odoratissima Mozaff. Ir J Med Aromatic Plants. 2010;26(2):147-56.
[5]Yousefzadeh Z, Karimi A. Anti-inflammatory Effects of Kelussia odoratissima in Rats Model of Rheumatoid Arthritis. Am J Ethnomed. 2014;1(1):50-5.
[6]Askari-Khorasgani O, Mortazaeinezhad F. Chemical composition of wild celery (Kelussia odoratissima) cultured in-vitro and from its local habitat. J Essent Oil Bear Pl. 2015;18(2):489-95.
[7]Asgary S, Naderi G, Dashti G, Paknahad Z. Effect of Amirkabiria odorastissima mozaffarian on the development and progression of fatty streaks in hypercholesterolemic rabbits. Phytother Res. 2004;18(5):370-2.
[8]Rabbani M, Sajjadi SE, Sadeghi M. Chemical composition of the essential oil from Kelussia odoratissima Mozaff and the evaluation of its sedative and anxiolytic effects in mice. Clinics (Sao Paulo) 2011;66(5):843-8.
[9]Sajjadi SE, Shokoohinia Y, Mehramiri P. Isolation and characterization of steroids, phthalide and essential oil of the fruits of Kelussia odoratissima Mozaff., an endemic mountain celery. Res Pharm Sci. 2013;8(1):35-41.
[10]Kheirandish M, Behbahani M. Evalution of cytotoxic effect of leaves, shoots, seeds, flowers and roots extracts of Kelussia odoratissima Mozaff grown in Kohrang and Freydonshahr regions. Res Med. 2014;38(4):221-5. [Persian]
[11]Cao YX, Zhang W, He JY, He LC, Xu CB. Ligustilide induces vasodilatation via inhibiting voltage dependent calcium channel and receptor-mediated Ca2+ influx and release. Vascul Pharmacol. 2006;45(3):171-6.
[12]Kerry N, Rice-Evans C. Peroxynitrite oxidises catechols to o-quinones. FEBS let. 1998;437(3):167-71.
[13]Lee TF, Lin YL, Huang YT. Studies on antiproliferative effects of phthalides from Ligusticum chuanxiong in hepatic stellate cells. Planta Med. 2007;73(6):527-34.
[14]Ahmadi F, Kadivar M, Shahedi M. Antioxidant activity of Kelussia odoratissima Mozaff. in model and food systems. Food Chem. 2007;105(1):57-64.
[15]Iravani M, Jaberol-Ansar Z. Kelussia odoratissima, an overthrowing plant in Central Zagros region. Tehran: Payam Sabz Publ. 2005. p. 39.
[16]Asgari S, Naderi Gh, Jafariyan A, Askari N, Behagh AR. Fibrinolytic activity of Amirkabiria Odoratissima Mozaffarian. J Med plants. 2005;1(13): 19-25.
[17]Asgary S, Naderi Gh, Dashti Gh, Paknahad Z. Effect of Amirkabiria odoratissima Mozaffarian on development and progression of fatty streaks in hypercholesterolemic rabbits. Phytotherapy Res. 2004;18(5):370-2.
[18]Ko FN, Huang TF, Teng CM. Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta. Biochim Biophys Acta. 1991;1115(1):69-74.
[19]Hojati MR, Sedighi Hafshejani M, Shahrani M. Effect of alcoholic extract of Kelussia odoratissima Mozaffarian on ileum contractions in rats. J Sabzevar Uni Med Sci. 2012;19(2):156-63.
[20]Jakovljevic V, Raskovic A, Popovic M, Sabo J. The effect of celery and parsley juices on pharmacodynamics activity of drugs involving cytochrome P450 in their metabolism. Eur J Drug Metab Pharmacokinet. 2002;27(3):153-6.
[21]Mehrabani M, Mehrabani M, Nematolahi H, Mehrabani M, Mansourinejad E, Raftari Sh. Effects of apple (Malus domestica Borkh.) diet on rat reproduction and sex ratio of offsprings. J Kerman Univ Med Sci. 2011;18(3):260-70.
[22]Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethno medicinal plants used by Player tribe from Tamil Nadu. BMC Complement Altern Med. 2006;6:35.
[23]Naderi F, Azhdari-Zarmehri H, Erami E, Sonboli A, Sofiabadi M, Mohammad-Zadeh M. The effect of Tanacetum sonbolii hydroalcholic extract on PTZ induced seizures in male mice. J Med Plants. 2012;11(4):193-201. [Persian]
[24]Gharib Naseri MK, Mard SA, Farbood Y. Effect of Anethum Graveolens fruit extract on rat uterus contractions. Iran J Basic Med Sci. 2006;8(4):263-70. [Persian]
[25]Gharib Naser MK, Mazlumi H, Goshayesh M, Vakilzade G, Heydari A. Anhispasmodic effect of Zataria multiflorn Boiss. Leuf Extract on the rat uterus. Ir J Pharmaceutical Res. 2006;5(2):131-36.
[26]Ohya Y, Sperelakis N. Fast Na+ and slow Ca2+ channels in single uterine muscle cells from pregnant rats. Am J Physiol. 1989;257(2 Pt 1):C408-12.
[27]Kim BK, Ozaki H, Lee SM, Karaki H. Increased sensitivity of rat myometrium to the increased contractile effect of platelet activating factor before delivery. Br J Pharmacol. 1995;115(7):1211-4.
[28]Sanborn B.M. Hormones and calcium: Mechanisms controlling uterine smooth muscle contractile activity. The Litchfield Lecture. Exp Physiol. 2001;86(2):223-37.
[29]Asadiyeh Shojaei Z, Ebrahimi A, Salimi M. Chemical composition of three ecotypes of wild celery (Kelussia odoratissima). J Herb Spice Med Pl. 2011;17(1):62-8.
[30]Bunel V, Antoine MH, Nortier J, Duez P, Stévigny C. Nephroprotective effects of ferulic acid, Z-ligustilide and E-ligustilide isolated from Angelica sinensis against cisplatin toxicity in vitro. Toxicol In Vitro. 2015;29(3):458-67.
[31]Salimi M, Ebrahimi A, Saei-Dehkordi S S, Shojaei Z. Extraction and Identification of Chemical Composition of Klussia odoritissima Mozaff. Iran J Med Aromatic Plants. 2010;26(2):147-56. [Persian]
[32]Phillippe M, Saunders T, Basa A. Intracellular mechanisms underlying prostaglandin F2α-stimulated phasic myometrial contractions. Am Physiol Society. 1997;273(4):E665-73.
[33]Beretta C, Cavalli M. A sheer pharmacologic approach to compare the contractile effects of PGF2α, DL-cloprostenol and D-cloprostenol on isolated uterine, tracheal, ileal and arterial smooth muscle preparations. Theriogenology. 2004;62(5):837-46.
[34]Narumiya S, Sugimoto Y, Ushikubi F. Prostanoid receptors: structures, properties and functions. Physiol Rev. 1999;79(4):1193-226.
[35]Gharib Naseri MK. Inhibitory effect of Zizyphus Spina Christi leaf extract on rat isolated uterus. Jundishapur Sci Med J. 2003;38(2):24-31. [Persian]
[2]Serashti M, Azari P, Rafiean M, Kheiri S. Use of herbal medicines by pregnant women in Shahr-e-Kord. J Reprod Infertility. 2006;7(2):125-31.
[3]Omidbaigi R, Sefidkon F, Saeedi K. Essential oil content and composition of Kelussia odoratissima Mozaff as an Iranian endemic plant. J Essent Oil Bearing Plants. 2008;11(6): 594-7.
[4]Salimi M, Ebrahimi A, Shojaee Asadieh Z, Saei Dehkordi SS. Essential oil composition of Kelussia odoratissima Mozaff. Ir J Med Aromatic Plants. 2010;26(2):147-56.
[5]Yousefzadeh Z, Karimi A. Anti-inflammatory Effects of Kelussia odoratissima in Rats Model of Rheumatoid Arthritis. Am J Ethnomed. 2014;1(1):50-5.
[6]Askari-Khorasgani O, Mortazaeinezhad F. Chemical composition of wild celery (Kelussia odoratissima) cultured in-vitro and from its local habitat. J Essent Oil Bear Pl. 2015;18(2):489-95.
[7]Asgary S, Naderi G, Dashti G, Paknahad Z. Effect of Amirkabiria odorastissima mozaffarian on the development and progression of fatty streaks in hypercholesterolemic rabbits. Phytother Res. 2004;18(5):370-2.
[8]Rabbani M, Sajjadi SE, Sadeghi M. Chemical composition of the essential oil from Kelussia odoratissima Mozaff and the evaluation of its sedative and anxiolytic effects in mice. Clinics (Sao Paulo) 2011;66(5):843-8.
[9]Sajjadi SE, Shokoohinia Y, Mehramiri P. Isolation and characterization of steroids, phthalide and essential oil of the fruits of Kelussia odoratissima Mozaff., an endemic mountain celery. Res Pharm Sci. 2013;8(1):35-41.
[10]Kheirandish M, Behbahani M. Evalution of cytotoxic effect of leaves, shoots, seeds, flowers and roots extracts of Kelussia odoratissima Mozaff grown in Kohrang and Freydonshahr regions. Res Med. 2014;38(4):221-5. [Persian]
[11]Cao YX, Zhang W, He JY, He LC, Xu CB. Ligustilide induces vasodilatation via inhibiting voltage dependent calcium channel and receptor-mediated Ca2+ influx and release. Vascul Pharmacol. 2006;45(3):171-6.
[12]Kerry N, Rice-Evans C. Peroxynitrite oxidises catechols to o-quinones. FEBS let. 1998;437(3):167-71.
[13]Lee TF, Lin YL, Huang YT. Studies on antiproliferative effects of phthalides from Ligusticum chuanxiong in hepatic stellate cells. Planta Med. 2007;73(6):527-34.
[14]Ahmadi F, Kadivar M, Shahedi M. Antioxidant activity of Kelussia odoratissima Mozaff. in model and food systems. Food Chem. 2007;105(1):57-64.
[15]Iravani M, Jaberol-Ansar Z. Kelussia odoratissima, an overthrowing plant in Central Zagros region. Tehran: Payam Sabz Publ. 2005. p. 39.
[16]Asgari S, Naderi Gh, Jafariyan A, Askari N, Behagh AR. Fibrinolytic activity of Amirkabiria Odoratissima Mozaffarian. J Med plants. 2005;1(13): 19-25.
[17]Asgary S, Naderi Gh, Dashti Gh, Paknahad Z. Effect of Amirkabiria odoratissima Mozaffarian on development and progression of fatty streaks in hypercholesterolemic rabbits. Phytotherapy Res. 2004;18(5):370-2.
[18]Ko FN, Huang TF, Teng CM. Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta. Biochim Biophys Acta. 1991;1115(1):69-74.
[19]Hojati MR, Sedighi Hafshejani M, Shahrani M. Effect of alcoholic extract of Kelussia odoratissima Mozaffarian on ileum contractions in rats. J Sabzevar Uni Med Sci. 2012;19(2):156-63.
[20]Jakovljevic V, Raskovic A, Popovic M, Sabo J. The effect of celery and parsley juices on pharmacodynamics activity of drugs involving cytochrome P450 in their metabolism. Eur J Drug Metab Pharmacokinet. 2002;27(3):153-6.
[21]Mehrabani M, Mehrabani M, Nematolahi H, Mehrabani M, Mansourinejad E, Raftari Sh. Effects of apple (Malus domestica Borkh.) diet on rat reproduction and sex ratio of offsprings. J Kerman Univ Med Sci. 2011;18(3):260-70.
[22]Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethno medicinal plants used by Player tribe from Tamil Nadu. BMC Complement Altern Med. 2006;6:35.
[23]Naderi F, Azhdari-Zarmehri H, Erami E, Sonboli A, Sofiabadi M, Mohammad-Zadeh M. The effect of Tanacetum sonbolii hydroalcholic extract on PTZ induced seizures in male mice. J Med Plants. 2012;11(4):193-201. [Persian]
[24]Gharib Naseri MK, Mard SA, Farbood Y. Effect of Anethum Graveolens fruit extract on rat uterus contractions. Iran J Basic Med Sci. 2006;8(4):263-70. [Persian]
[25]Gharib Naser MK, Mazlumi H, Goshayesh M, Vakilzade G, Heydari A. Anhispasmodic effect of Zataria multiflorn Boiss. Leuf Extract on the rat uterus. Ir J Pharmaceutical Res. 2006;5(2):131-36.
[26]Ohya Y, Sperelakis N. Fast Na+ and slow Ca2+ channels in single uterine muscle cells from pregnant rats. Am J Physiol. 1989;257(2 Pt 1):C408-12.
[27]Kim BK, Ozaki H, Lee SM, Karaki H. Increased sensitivity of rat myometrium to the increased contractile effect of platelet activating factor before delivery. Br J Pharmacol. 1995;115(7):1211-4.
[28]Sanborn B.M. Hormones and calcium: Mechanisms controlling uterine smooth muscle contractile activity. The Litchfield Lecture. Exp Physiol. 2001;86(2):223-37.
[29]Asadiyeh Shojaei Z, Ebrahimi A, Salimi M. Chemical composition of three ecotypes of wild celery (Kelussia odoratissima). J Herb Spice Med Pl. 2011;17(1):62-8.
[30]Bunel V, Antoine MH, Nortier J, Duez P, Stévigny C. Nephroprotective effects of ferulic acid, Z-ligustilide and E-ligustilide isolated from Angelica sinensis against cisplatin toxicity in vitro. Toxicol In Vitro. 2015;29(3):458-67.
[31]Salimi M, Ebrahimi A, Saei-Dehkordi S S, Shojaei Z. Extraction and Identification of Chemical Composition of Klussia odoritissima Mozaff. Iran J Med Aromatic Plants. 2010;26(2):147-56. [Persian]
[32]Phillippe M, Saunders T, Basa A. Intracellular mechanisms underlying prostaglandin F2α-stimulated phasic myometrial contractions. Am Physiol Society. 1997;273(4):E665-73.
[33]Beretta C, Cavalli M. A sheer pharmacologic approach to compare the contractile effects of PGF2α, DL-cloprostenol and D-cloprostenol on isolated uterine, tracheal, ileal and arterial smooth muscle preparations. Theriogenology. 2004;62(5):837-46.
[34]Narumiya S, Sugimoto Y, Ushikubi F. Prostanoid receptors: structures, properties and functions. Physiol Rev. 1999;79(4):1193-226.
[35]Gharib Naseri MK. Inhibitory effect of Zizyphus Spina Christi leaf extract on rat isolated uterus. Jundishapur Sci Med J. 2003;38(2):24-31. [Persian]