ARTICLE INFO

Article Type

Original Research

Authors

Zare Bidaki   M. (1)
Arab   M. (*)
Khazaei   M. (2)
Afkar   E. (3)
Zardast   M. (4)






(*) Clinical Biochemistry Department, Medicine Faculty, Birjand University of Medical Sciences, Birjand, Iran
(1) “Hepatitis Research Center” and “Medical Microbiology Department, Paramedics Faculty, Birjand University of Medical Sciences, Birjand, Iran
(2) Medical Microbiology Department, Paramedics Faculty, Birjand University of Medical Sciences, Birjand, Iran
(3) Deputy of Research & Technology, Birjand University of Medical Sciences, Birjand, Iran
(4) Pathology Department, Medicine Faculty, Birjand University of Medical Sciences, Birjand, Iran

Correspondence

Address: South Khorasan, Birjand, Ghaffari Street, Birjand University of Medical Sciences, Medicine Faculty, Clinical Biochemistry Department
Phone: +985632395507
Fax: +985632440388
minaarab70@yahoo.com

Article History

Received:   January  5, 2015
Accepted:   January 25, 2015
ePublished:   September 20, 2015

ABSTRACT

Aims According to the worldwide development of antibiotic resistance in recent years, the tendency of using herbal medicine has grown and plants derived antibacterial substances have found many applications in controlling microorganisms. Thyme (Zataria multiflora Boiss.) is one of the medicinal herbs which its pharmaceutical and antibacterial effects have been a matter of research and debate in recent years. This research aimed to study antibacterial effects of Zataria multiflora Boiss. essence against 8 standard pathogenic bacterial species.
Materials & Methods In this experimental study, the essential oil was extracted by steam distillation using Clevenger apparatus. Using broth micro-dilution testing, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of thyme essential oil were determined against 8 standard pathogenic bacterial species. Each exam was repeated three times. The results were analyzed by SPSS 16 software using one-way ANOVA and Tukey statistical tests.
Findings The average of MIC for Zataria multiflora Boiss. essential oil showed a significant difference among 8 different bacterial species (p<0.001). The highest growth inhibitory effect was found against Bacillus cereus and the least one was against Pseudomonas aeruginosa. There was no significant difference among the averages of MBCs (p=0.073).
Conclusion Zataria multiflora Boiss. essential oil has significant antibacterial effect.

Keywords: Zataria multiflora Boiss., Oils, Volatile , Anti-Bacterial Agents , Microbial Sensitivity Tests ,

CITATION LINKS

[1]Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-82.
[2]Ebrahimi A, Khayami M, Nejati V. Comparison of antimicrobial effect of different parts of quercus persica against escherichia coli O157:H7. Q Horizon Med Sci. 2012;17(4):11-7. [Persian]
[3]Ates DA, Erdogrul OT. Antimicrobial activities of various medicinal and commercial plant extracts. Turk J Biol. 2003;27:157-62.
[4]Sedighi J, Maftoun F, Ziaei G. Herbal medicine: Knowledge, attitude and practice in Tehran. J Med Plant. 2005;4(13):11-8.
[5]Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Braz J Med Biol Res. 2000;33(2):179-89.
[6]Abdollahi B, Ziaei F, Shabankhani B, Azadbakht M. Effect of essential oils of Artemisia aucheri Boiss, Zataria multiflora Boiss, and Myrtus communis L. on Trichomonas vaginalis. Iran J Pharm Res. 2004;3 suppl 2:35-40.
[7]Azadbakht M, Azadbakht M. Five common herbal remedy anti-protozoan. J Mazandaran Uni Med Sci. 2008;18(67):118-32. [Persian]
[8]Bagheri A, Naghdi Badi H, Maki Zadeh M, Hemati AR, Movahedian F. Evaluation of using herbal medicine in Isfahan women population. J Med Plant. 2005;4(15):81-92.
[9]Smith Palmer A, Stewart J, Fyfe L. The potential application of plant essential oils as natural food preservatives in soft cheese. Food Microbiol. 2001;18(4):463-70.
[10]Sharififar F, Moshafi MH, Mansouri SH, Khodashenas M, Khoshnoodi M. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control. 2007;18(7):800-5.
[11]Eftekhar F, Zamani S, Yusefzadi M, Hadian J, Nejad Ebrahimi S. Antibacterial activity of Zataria multiflora Boiss essential oil against extended spectrum β lactamase produced by urinary isolates of Klebsiella pneumonia. Jundishapur J Microbiol. 2011;4(5):S43-9.
[12]Saei Dehkordi SS, Tajik H, Moradi M, Khalighi Sigaroodi F. Chemical composition of essential oils in Zataria multiflora Boiss. From different parts of Iran and their radical scavenging and antimicrobial activity. Food Chem Toxicol. 2010;48(6):1562-7.
[13]Tiwari BK, Valdramidis VP, O’Donnell CP, Muthukumarappan K, Bourke P, Cullen PJ. Application of natural antimicrobials for food preservation. J Agric Food Chem. 2009;57:5987-6000.
[14]Mansoura A, Enayatb K, Neda MS, Behzad A. Antibacterial effect and physicochemical properties of essential oil of Zataria multiflora Boiss. Asian Pac J Trop Med. 2010;6(3):439-42.
[15]Ramezani M, Hosseinzadeh H, Samizadeh S. Antinociceptive effects of Zataria multiflora Boiss fractions in mice. J Ethnopharmacol. 2004;91(1):167-70.
[16]Sefidkon F, Rahimi Bidgoli A. Quantitative and qualitative variation of essential oil of Thymus kotschyanus by different methods of distillation and stage of plant growth. Iran J Med Aroma Plant Res. 2003;15:1-22. [Persian]
[17]Basti AA, Misaghi A, Ebrahimzade E, Abbasifar R, Radmehr SH, Akhondzade S. Effect of Zataria multiflora Boiss. essential oil on lag phase of growth of Staphylococcus aureus in a brain heart infusion broth. J Med Plant. 2004;3(11):42-7. [Persian]
[18]Rahnama M, Razavi Rohani SM, Tajik H, Khaleghi Sigarudi F, Rezazad Bari M. Effects of Zataria multiflora Boiss. essential oil and nisin, alone and in combination against listeria monocytogen in BHI broth. J Med Plant. 2009;4(32):120-31. [Persian]
[19]Mahboubi M, Bidgoli FG. Antistaphylococcal activity of Zataria multiflora essential oil and its synergy with vancomycin. Phytomedicine. 2010;17(7):548-50.
[20]Hosseinzadeh A, Mohajerfar T, Akhondzadeh Basti A, Khanjari A, Gandomi Nasrabadi H, Misaghi A, et al. Determination of minimum inhibitory concentration (MIC) of Zataria multiflora Boiss. essential oil and lysozyme on E. coli O157: H7. J Med Plant. 2011;1(8):208-17. [Persian]
[21]Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol. 1999;86(6):985-90.
[22]Azizkhani M, Misaghi A, Akhondzadeh Basti A, Gandomi Nasrabadi H, Hosseini H. Effect of Zataria multiflora Boiss. essential oil on growth and enterotoxin e production of Staphylococcus aurous ATCC 29213. J Med Plant. 2012;4(44):185-93. [Persian]
[23]Mashak Z, Moradi B, Moradi B. The Combined Effect of Zataria multiflora Boiss. and Cinnamomum zeylanicum Nees. essential Oil on the Growth of Bacillus cereus in a Food Model System. J Med Plant. 2012;2(42):62-73. [Persian]
[24]Hashemi A, Shams S. Barati M, Samedani A. Antibacterial effects of methanolic extracts of Zataria multiflora, Myrtus communis and Peganum harmala on Pseudomonas aeruginosa producing ESBL. Arak Med Univ J. 2011;14(4):104-12. [Persian]
[25]Moshafi MH, Mansuri S, Sharififar F, Khoshnoodi M. Antibacterial and antioxidant effects of the essential oil and extract of Zataria Multiflora Boiss. J Kerman Univ Med Sci. 2007;14(1):33-43. [Persian]
[26]Moosavy MH, Basti AA, Misaghi A, Jabari Khameneh H, Karim G, Zahraei Salehi T. The survey of effect of Zataria multiflora Boiss. essential oil on the growth of salmonella typhimurium in a commercial barley soup. J Med Plants. 2010;2(34):109-16. [Persian]
[27]Ravanshad S, Basiri E, Dastgheib B. Antimicrobial activity of different concentrations of essential oil of Zataria multiflora on enterococcus faecalis. Shiraz Univ Dent J. 2007;8(1):28-36. [Persian]
[28]Zahraei Salehi T, Vojgani M, Bayat M, Torshizi H, Akhondzadeh A. Determination of Minimum Inhibitory Concentration (MIC) of Extract of Zataria multiflora, against the clinical isolates of Streptococcus agalactiae Staphylococcus, aureus and E. coli. J Fac Vet Med Univ Tehran. 2005;60(2):107-10. [Persian]
[29]Gandomi Nasrabadi H, Misaghi A, Akhondzadeh Basti A, Khosravi A, Bokaei S, Abbasifar R. Effects of Zataria multiflora Boiss. essential oil on aspergillus flavus. J Med Plant. 2008;3(27):45-51. [Persian]
[30]Akbari S. Antifungal activity of Thymus vulgaris L. and Origanum vulgare L. Against Fluconazol-Resistant and Susceptible Candida albicans Isolates. J Med Plant. 2007;1(Suppl 3):53-62. [Persian]
[31]Moghimipour E, Aghel N, Ameri A, Saadatzadeh A. Anti-dermatophyte cream formulation of the hydroalcolic extract of Zataria multifora Boiss. Iran J Basic Med Sci. 2007;10(1):36-45. [Persian]
[32]Sahebani NA, Movahhed A, Farsangi MH. Cytotoxic effects of thyme on giardia cyst in vitro. South Med J. 2002;4(2):88-95. [Persian]
[33]Azadbakht M, Ziai H, Abdullahi F, Shabankhani B. Effect of essential oils of Artemisia. Zataria and Myrtus on Trichomonas vaginalis. J Med Plant. 2003;4(8):35-40. [Persian]
[34]Ashtaral Nakhai L, Mohammadirad A, Yasa N, Minaie B, Nikfar SH, Ghazanfari G, et al. Benefits of Zataria multiflora Boiss in experimental model of mouse inflammatory bowel disease. Evid Based Complement Alternat Med. 2007;4(1):43-50.
[35]Hosseinzadeh H, Ramezani M, Salmani G. Antinociceptive, anti-inflammatory and acute toxicity effects of Zataria multiflora Boiss extracts in mice and rats. J Ethnopharmacol. 2000;73(3):379-85.
[36]Sabetsarvestani MM, Sharafzadeh Sh, Alizadeh A, Rezaeian AA. Total phenolic content, antioxidant activity and antifungal property in two parts of garden thyme shoot. Int J Farming Allied Sci. 2013;2(22):1017-22.