@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2017;23(2):149-155
ISSN: 2252-0805 The Horizon of Medical Sciences 2017;23(2):149-155
Effect of Aqueous Extract of Launaea acanthodes on DNA Oxidative Damage and Antioxidant Enzymes Activities in Diabetic Rats Testes Tissue
ARTICLE INFO
Article Type
Original ResearchAuthors
Tafakkor S. (1)Sadoughi S.D. (* )
Rahbarian R. (1)
(* ) Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran
(1) Biology Department, Sciences Faculty, Tehran Branch, Payam-e-Noor University, Tehran , Iran
Correspondence
Address: Biology Department, Sciences Faculty, Payam-e-Noor University, Mo’allem Boulevard, Mashhad, Iran. Post Box: 91735-433Phone: +98 (51) 38683900
Fax: +98 (51) 38683001
damoon.sadoughi@mshdiau.ac.ir
Article History
Received: June 14, 2016Accepted: July 25, 2016
ePublished: March 25, 2017
ABSTRACT
Aims
Oxidative stress plays an important role in the initiation and progression of diabetes. Noticing the antioxidant and antidiabetes effects of Launaea acanthodes, the aim of the study was to determine the effects of the aqueous extract of the herb on DNA oxidative damage and the activities of antioxidant enzyms in the testicular tissue of diabetic rats.
Materials & Methods In the experimental study, 32 male Wistar rats were studied. The rats were randomly divided into four 8-rat groups including healthy control, diabetic control, and experimental diabetic groups treated by aqueous extract of Launaea acanthodes. Diabetes was induced in diabetic control and diabetic experimental groups via one alloxan intraperitoneal injection. 100 and 200mg/Kg aqueous extract of Launaea acanthodes were injected as intraperitoneal in diabetic experimental groups at alternate days for one month, while the control groups received only strile distilled water via injection. At the end of the study, the activities of antioxidant enzyms and the levels of 8-hydroxydeoxyguanosine and malondialdehyde were assessed in the testicular tissue. Data was analyzed by SPSS 20 software using one-way ANOVA and Tukey’s post-hoc tests.
Findings The tissue activities of superoxide dismutase, glutathione peroxidase, and catalase enzymes significantly increased in the experimental groups treated by 100 and 200mg/Kg aqueous extract of Launaea acanthodes as dose-dependent compared to diabetic control group, while the levels of 8-hydroxideoxiguanosine and malondialdehyde significantly decreased (p<0.05).
Conclusion Dose-dependent administration of aqueous extract of Launaea acanthodes increases the antioxidant capacity, while reducing lipidic peroxidation and DNA oxidative damage in the testicular tissue of rats.
Materials & Methods In the experimental study, 32 male Wistar rats were studied. The rats were randomly divided into four 8-rat groups including healthy control, diabetic control, and experimental diabetic groups treated by aqueous extract of Launaea acanthodes. Diabetes was induced in diabetic control and diabetic experimental groups via one alloxan intraperitoneal injection. 100 and 200mg/Kg aqueous extract of Launaea acanthodes were injected as intraperitoneal in diabetic experimental groups at alternate days for one month, while the control groups received only strile distilled water via injection. At the end of the study, the activities of antioxidant enzyms and the levels of 8-hydroxydeoxyguanosine and malondialdehyde were assessed in the testicular tissue. Data was analyzed by SPSS 20 software using one-way ANOVA and Tukey’s post-hoc tests.
Findings The tissue activities of superoxide dismutase, glutathione peroxidase, and catalase enzymes significantly increased in the experimental groups treated by 100 and 200mg/Kg aqueous extract of Launaea acanthodes as dose-dependent compared to diabetic control group, while the levels of 8-hydroxideoxiguanosine and malondialdehyde significantly decreased (p<0.05).
Conclusion Dose-dependent administration of aqueous extract of Launaea acanthodes increases the antioxidant capacity, while reducing lipidic peroxidation and DNA oxidative damage in the testicular tissue of rats.
CITATION LINKS
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[14]Sepehri-Moghadam H, Rahbarian R, Sadoughi SD. The effect of aqueous extract of Launaea acanthodes (Boiss.) O. Kuntze on the serum level of insulin and blood glucose and histomorphological changes of pancreas in diabetic rats. Feyz. 2015;19(1):30-7. [Persian]
[15]Behnam-Rassouli M, Ghayour N, Ghayour M, Ejtehadi M. Investigating the effects of hydro-alcoholic extract of Launaea acanthodes on the serum levels of glucose, insulin, lipids and lipoproteins in stereptozotocin induced type I diabetic rats. Arak Univ Med Sci J. 2012;14(6):48-56. [Persian]
[16]Rahbarian R, Sepehri Moghadam H, Sadoughi SD. Effect of aqueous extract of Launaea acanthodes on open skin wound in diabetic rats. Horizon Med Sci. 2016;21(1):1-11. [Persian]
[17]Malek-Mohammadi R, Roghani M, Salami M. The effect of aqueous extracts of Melissa officinalis on the oxidative stress indices in the midbrain tissue. Feyz. 2015;19(1):8-14. [Persian]
[18]Zargari F, Ghorbanihaghjo A, Babaei H, Farajnia S, Hayati Roodbari N. The effect of hydroalcoholic extract of Nasturtium officinale R.Br on antioxidant status and DNA damage in liver and kidney rats exposed to arsenic. Med J Tabriz Univ Med Sci. 2014;36(3):44-51. [Persian]
[19]Roghani M, Baluchnejadmojarad T, Roghani Dehkordi F. Effect of chronic administration of Silymarin on oxidative stress markers in renal tissue of diabetic rats. J Gorgan Uni Med Sci. 2012;14(2):10-6. [Persian]
[20]Kazemi A, Zarei Mahmoudabadi A, Fasihi Ramandi M, Rasouli Vani, Saberi M. Evaluating oxidative stress factors induced by chlorpyrifos poisoning in plasma of wistar rat. J Shahid Sadoughi Univ Med Sci. 2014;22(2):1079-89. [Persian]
[21]Sameni H, Kavakebian F, Tabriziamjad M, Bandegi A, Yousefi B, Taherian AA. Effects of hydroalcoholic extract of propolis on oxidative stress indices of rat fetal brain induced by chronic prenatal stress. Koomesh. 2014;15(4):482-92. [Persian]
[22]Chen CH, Lu Y, Chen Y, Lee W, Lu CH, Chen Y, et al. Hemopexin is up-regulated in plasma from type 1 diabetes mellitus patients: Role of glucose-induced ROS. J Proteomics. 2012;75(12):3760-77
[23]Kuo CW, Shen CJ, Tung YT, Chen HL, Chen YH, Chang WH, et al. Extracellular superoxide dismutase ameliorates streptozotocin-induced rat diabetic nephropathy via inhibiting the ROS/ERK1/2 signaling. Life Sci. 2015;135:77-86.
[24]Varsha MK, Thiagarajan R, Manikandan R, Dhanasekaran G. Vitamin K1 alleviates streptozotocin-induced type 1 diabetes by mitigating free radical stress, as well as inhibiting NF-κB activation and iNOS expression in rat pancreas. Nutrition. 2015;31(1):214-22.
[25]Salimnejad R, Jalali M, Nikravesh MR, Fazel AR. Effect of garlic aqueous extract on markers of oxidative stress in diabetic rats testes. J Rafsanjan Univ Med Sci. 2014;13(4):371-82. [Persian]
[26]Goodarzi M T, Navidi Abaspour A, Rezaie M, Baba Ahmadi Rezaie H, Ansari M. Oxidative damage to DNA and lipids: Correlation with protein glycation in patients with type 1 diabetes. Sci J Hamadan Univ Med Sci. 2008;14(4):33-7. [Persian]
[27]Dong D, Yu J, Wu Y, Fu N, Villela NA, Yang P. Maternal diabetes triggers DNA damage and DNA damage response in neurulation stage embryos through oxidative stress. Biochem Biophys Res Commun. 2015;467(2):407-12.
[28]Kolesnikova LI, Darenskaya MA, Semenova NV, Grebenkina LA, Suturina LV, Dolgikh MI, et al. Lipid peroxidation and antioxidant protection in girls with type 1 diabetes mellitus during reproductive system development. Medicina. 2015;51(2):107-11.
[29]Nasirzadeh MR, Heykalabadi M, Nourazar A. Effect of alcoholic extract of euphorbia cyparissias on serum glucose and antioxidant enzymes level in diabetic male rats. J Ardabil Univ Med Sci. 2014;14(2):193-202. [Persian]
[30]Nasirzadeh MR, Nourazar A, Khalili-Moghadam S, Mohammadiani M. Effect of alcoholic extract of Euphorbia cyparissias on the brain antioxidant enzymes in streptozotocin-induced diabetic male rats. FEYZ. 2014;18(3):194-200. [Persian]
[31]Jalali M, Behnam Rassouli M, Tehranipour M, Ghayour N, Khayatzadeh J, Jannati H. Study of the effects of hyperglycemia and Launaea acanthodes extract administration on disorders of liver function in rats. Physiol Pharmacol. 2012;15(4):562-71. [Persian]
[2]Rashidi A, Kalalian Moghaddam H, Norouzi P. Effect of root kudzu on biochemical parameters in streptozotocin-induced diabetic rats. J Knowledge Health. 2015;10(2):18-23.
[3]Jakus V. The role of free radicals, oxidative stress and antioxidant systems in diabetic vascular disease. Bratisl Lek Listy. 2000;101(10):541-51.
[4]Rochette L, Zeller M, Cottin Y, Vergely C. Diabetes, oxidative stress and therapeutic strategies. Biochim Biophys Acta. 2014;1840(9):2709-29.
[5]Saunders D, Powers AC. Replicative capacity of β-cells and type 1 diabetes. J Autoimmun. 2016;71:59-68.
[6]Gebicki JM. Oxidative stress, free radicals and protein peroxides. Arch Biochem Biophys. 2016;595:33-9.
[7]Rahbarian R, Sepehri-Moghadam H, Sadoughi SD. The effects of aqueous extract of Launaea acanthodes on oxidative stress parameters of red blood cells in diabetic rats. J Rafsanjan Univ Med Sci. 2016;14(10):865-78. [Persian]
[8]Wu LL, Chiou CC, Chang PY, Wu JT. Urinary 8-OHdG: A marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta. 2004;339(1-2):1-9.
[9]Setyaningsih Y, Husodo AH, Astuti I. Detection of urinary 8-hydroxydeoxyguanosine (8-OHdG) levels as a biomarker of oxidative DNA damage among home industry workers exposed to chromium. Procedia Environ Sci. 2015;23:290-6.
[10]Hajinejad Boshroue R, Behnam-Rassouli M, Tehranipour M, Gheybi F, Hajinejad Sh, Elahi Moghaddam Z. The effects of hydro- alcoholic extract of Launaea acanthodes on the blood, urine albumin and bilirubin levels in male hyperglycemic wistar rat. Iran J Endocrinol Metab. 2013;15(2):190-233. [Persian]
[11]Piazza L, Bertini S, Milany J. Extraction and structural characterization of the polysaccharide fraction of Launaea acanthodes gum. Carbohydr Polym. 2010;79(2):449-54.
[12]Karimidokht Shahrbabaki A, Oryan SH, Parivar K. Anticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice. J Qazvin Univ Med Sci. 2009;13(1):14-20. [Persian]
[13]Rahbarian R, Sepehri Moghadam H, Sadoughi SD. Effect of aqueous extract of Launaea acanthodes on testicular tissue and sperm parameters in alloxan-induced diabetic rats. Horizon Med Sci. 2015;21(1):21-9. [Persian]
[14]Sepehri-Moghadam H, Rahbarian R, Sadoughi SD. The effect of aqueous extract of Launaea acanthodes (Boiss.) O. Kuntze on the serum level of insulin and blood glucose and histomorphological changes of pancreas in diabetic rats. Feyz. 2015;19(1):30-7. [Persian]
[15]Behnam-Rassouli M, Ghayour N, Ghayour M, Ejtehadi M. Investigating the effects of hydro-alcoholic extract of Launaea acanthodes on the serum levels of glucose, insulin, lipids and lipoproteins in stereptozotocin induced type I diabetic rats. Arak Univ Med Sci J. 2012;14(6):48-56. [Persian]
[16]Rahbarian R, Sepehri Moghadam H, Sadoughi SD. Effect of aqueous extract of Launaea acanthodes on open skin wound in diabetic rats. Horizon Med Sci. 2016;21(1):1-11. [Persian]
[17]Malek-Mohammadi R, Roghani M, Salami M. The effect of aqueous extracts of Melissa officinalis on the oxidative stress indices in the midbrain tissue. Feyz. 2015;19(1):8-14. [Persian]
[18]Zargari F, Ghorbanihaghjo A, Babaei H, Farajnia S, Hayati Roodbari N. The effect of hydroalcoholic extract of Nasturtium officinale R.Br on antioxidant status and DNA damage in liver and kidney rats exposed to arsenic. Med J Tabriz Univ Med Sci. 2014;36(3):44-51. [Persian]
[19]Roghani M, Baluchnejadmojarad T, Roghani Dehkordi F. Effect of chronic administration of Silymarin on oxidative stress markers in renal tissue of diabetic rats. J Gorgan Uni Med Sci. 2012;14(2):10-6. [Persian]
[20]Kazemi A, Zarei Mahmoudabadi A, Fasihi Ramandi M, Rasouli Vani, Saberi M. Evaluating oxidative stress factors induced by chlorpyrifos poisoning in plasma of wistar rat. J Shahid Sadoughi Univ Med Sci. 2014;22(2):1079-89. [Persian]
[21]Sameni H, Kavakebian F, Tabriziamjad M, Bandegi A, Yousefi B, Taherian AA. Effects of hydroalcoholic extract of propolis on oxidative stress indices of rat fetal brain induced by chronic prenatal stress. Koomesh. 2014;15(4):482-92. [Persian]
[22]Chen CH, Lu Y, Chen Y, Lee W, Lu CH, Chen Y, et al. Hemopexin is up-regulated in plasma from type 1 diabetes mellitus patients: Role of glucose-induced ROS. J Proteomics. 2012;75(12):3760-77
[23]Kuo CW, Shen CJ, Tung YT, Chen HL, Chen YH, Chang WH, et al. Extracellular superoxide dismutase ameliorates streptozotocin-induced rat diabetic nephropathy via inhibiting the ROS/ERK1/2 signaling. Life Sci. 2015;135:77-86.
[24]Varsha MK, Thiagarajan R, Manikandan R, Dhanasekaran G. Vitamin K1 alleviates streptozotocin-induced type 1 diabetes by mitigating free radical stress, as well as inhibiting NF-κB activation and iNOS expression in rat pancreas. Nutrition. 2015;31(1):214-22.
[25]Salimnejad R, Jalali M, Nikravesh MR, Fazel AR. Effect of garlic aqueous extract on markers of oxidative stress in diabetic rats testes. J Rafsanjan Univ Med Sci. 2014;13(4):371-82. [Persian]
[26]Goodarzi M T, Navidi Abaspour A, Rezaie M, Baba Ahmadi Rezaie H, Ansari M. Oxidative damage to DNA and lipids: Correlation with protein glycation in patients with type 1 diabetes. Sci J Hamadan Univ Med Sci. 2008;14(4):33-7. [Persian]
[27]Dong D, Yu J, Wu Y, Fu N, Villela NA, Yang P. Maternal diabetes triggers DNA damage and DNA damage response in neurulation stage embryos through oxidative stress. Biochem Biophys Res Commun. 2015;467(2):407-12.
[28]Kolesnikova LI, Darenskaya MA, Semenova NV, Grebenkina LA, Suturina LV, Dolgikh MI, et al. Lipid peroxidation and antioxidant protection in girls with type 1 diabetes mellitus during reproductive system development. Medicina. 2015;51(2):107-11.
[29]Nasirzadeh MR, Heykalabadi M, Nourazar A. Effect of alcoholic extract of euphorbia cyparissias on serum glucose and antioxidant enzymes level in diabetic male rats. J Ardabil Univ Med Sci. 2014;14(2):193-202. [Persian]
[30]Nasirzadeh MR, Nourazar A, Khalili-Moghadam S, Mohammadiani M. Effect of alcoholic extract of Euphorbia cyparissias on the brain antioxidant enzymes in streptozotocin-induced diabetic male rats. FEYZ. 2014;18(3):194-200. [Persian]
[31]Jalali M, Behnam Rassouli M, Tehranipour M, Ghayour N, Khayatzadeh J, Jannati H. Study of the effects of hyperglycemia and Launaea acanthodes extract administration on disorders of liver function in rats. Physiol Pharmacol. 2012;15(4):562-71. [Persian]