@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2017;23(1):49-53
ISSN: 2252-0805 The Horizon of Medical Sciences 2017;23(1):49-53
Effect of Intraperitoneal Injection of Hydroalcoholic Extract of Ducrosia anethifolia on Pentylenetetrazol-Induced Anticonvulsion in Male Wistar Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Nyasty F. (1)Oryan Sh. (1)
Sofiabadi M. (*)
Eslimi Esfahani D. (1)
(*) Department of Physiology, Medicine Faculty, Qazvin University of Medical Sciences, Qazvin, Iran
(1) Animal Science Department, Faculty of Sciences, Khwarizmi University, Tehran, Iran
Correspondence
Address: Department of Physiology, Medicine Faculty, Qazvin University of Medical Sciences, Bahonar Street, Qazvin, Iran. Postal Code: 3419759811Phone: +98 (28) 33336001
Fax: +98 (28) 33324970
mohasofi@yahoo.com
Article History
Received: May 24, 2016Accepted: October 16, 2016
ePublished: January 19, 2017
ABSTRACT
Aims
Since the common medications cannot completely heal the epilepsy, and the long-term consumption of such medications leads to some side effects, it is very important to achieve more effective medications. Therefore, it is necessary to notice the traditional medicine and herbal medications with low side effects, as well as to determine their effective doses. The aim of this study was to investigate the effects of intra-peritoneal injection of the hydro-alcoholic extract of Ducrosia anethifolia on pentilenetetrazol-induced (PTZ-induced) seizures in male Wistar rats.
Materials & Methods In the experimental study, 48 male Wistar rats were studied. The rats were divided into six 8-rat groups including control group (receiving saline), groups receiving 0.25, 0.5, 1, and 2mg/kg Ducrosia anethifolia extract via intra-peritoneal injection, and positive control group (receiving 1mg/Kg diazepam as an antiepileptic medication). 30min latter, and to produce the epileptic seizures, 80mg/Kg pantilenetetrazol was used via intra-peritoneal injection. Then, the convulsive behaviors of the animal were recorded. Data was analyzed by SPSS 16 software using one-way ANOVA and Tukey’s post-hoc tests.
Findings The pre-treatment done by the different doses of Ducrosia anethifolia lengthened the time interval before the beginning of the seizure attacks. The lengthened interval measure in 2mg/Kg extraction group was significant compared to control group. In addition, the different doses of Ducorsia anethifolia extract reduced the dose-dependent lengths of tunic seizures and tonic-clonic seizures, as well as the total dose-dependent seizure length.
Conclusion Intra-peritoneal injection of the hydro-alcoholic extract of Ducorsia anethifolia effectively reduces the PTZ-induced seizure manifestations in the male Wistar rats.
Materials & Methods In the experimental study, 48 male Wistar rats were studied. The rats were divided into six 8-rat groups including control group (receiving saline), groups receiving 0.25, 0.5, 1, and 2mg/kg Ducrosia anethifolia extract via intra-peritoneal injection, and positive control group (receiving 1mg/Kg diazepam as an antiepileptic medication). 30min latter, and to produce the epileptic seizures, 80mg/Kg pantilenetetrazol was used via intra-peritoneal injection. Then, the convulsive behaviors of the animal were recorded. Data was analyzed by SPSS 16 software using one-way ANOVA and Tukey’s post-hoc tests.
Findings The pre-treatment done by the different doses of Ducrosia anethifolia lengthened the time interval before the beginning of the seizure attacks. The lengthened interval measure in 2mg/Kg extraction group was significant compared to control group. In addition, the different doses of Ducorsia anethifolia extract reduced the dose-dependent lengths of tunic seizures and tonic-clonic seizures, as well as the total dose-dependent seizure length.
Conclusion Intra-peritoneal injection of the hydro-alcoholic extract of Ducorsia anethifolia effectively reduces the PTZ-induced seizure manifestations in the male Wistar rats.
CITATION LINKS
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[13]Ghiasi S, Vaezi GH, Keramati K. Effects of ICV injection of alcoholic extract of Hypericum Perforatum on fear behavior in presence pentylenetetrazole (PTZ) in adult male rat. J Ilam Univ Med Sci. 2010;17(4):36-44. [Persian]
[14]Avallone R, Zanoli P, Corsi L, Cannazza G, Baraldi M. Benzodiazepine-like compounds and GABA in flower heads of Matricaria chamomilla. Phytother Res. 1996;10:177-9.
[15]Duarte FS, Marder M, Hoeller AA, Duzzioni M, Mendes BG, Pizzolatti MG, et al. Anticonvulsant and anxiolytic-like effects of compounds isolated from Polygala sabulosa (Polygalaceae) in rodents: In vitro and in vivo interactions with benzodiazepine binding sites. Psychopharmacology (Berl). 2008;197(3):60-351.
[16]Cadirci E, Suleyman H, Gurbuz P, KruuzumUA, Guvenalp Z, Demirezer L. Anti-inflammatory effects of different extracts from three Salvia species. Turk J Biol. 2011;35:59-64.
[17]Garavand S, Keramati K, Zendehdel M, Jadidoleslami M, Garavand S. Effect of intracerebroventricular injection of flunixin meglumine on PTZ-induced seizures in male rats. Physiol Pharma. 2010;14(1):34-40. [Persian]
[18]Lasoń W, Chlebicka M, Rejdak K. Research advances in basic mechanisms of seizures and antiepileptic drug action.Pharmacol Rep. 2013;65(4):787-801.
[19]Sayyah M, Faraji H, Dehghani A, Bakhtiari H, Kamalinejad M, Narenjkar J. Screening of the anticonvulsant potential of some common medicinal plants of Iran in pentylenetetrazole and maximal electroshock seizure models in male mice. Physiol Pharma. 2011;15(1):66-71. [Persian]
[20]Rebrov I, Karpova M, Andreev A, Kuzina O, Kalinina M, Abrosimov IY, et al. Effect of single injection of pentylenetetrazole in a subconvulsive dose on Cl-conductance of the GABAA-receptor complex. Bull Exp Biol Med. 2004;137(1):6-13.
[21]Werz MA, Macdonald RL. Dynorphin reduces voltage-dependent calcium conductance of mouse dorsal root ganglion neurons. Neuropept. 1984;5(1-3):253-6.
[22]Li K, Xu E. The role and the mechanism of γ-aminobutyric acid during central nervous system development. Neurosci Bull. 2008;24(3):195-200.
[23]Janssen A, Scheffer J, Svendsen AB, Aynehchi Y. The essential oil of Ducrosia anethifolia (DC.) Boiss. Pharm Weekbl. 1984;6(4):60-157.
[24]Chebib M, Johnston GA. GABA-activated ligand gated ion channels: Medicinal chemistry and molecular biology. J Med Chem. 2000;43(8):1427-47.
[25]Jiang JG, Huang XJ, Chen J, Lin QS. Comparison of the sedative and hypnotic effects of flavonoids, saponins, and polysaccharides extracted from Semen Ziziphus jujube. Nat Prod Res. 2007;21(4):20-31.
[26]Karimidokht SA, Oryan S, Parivar K. Anticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice. J Qazvin Univ Med Sci. 2009;13(1):14-20. [Persian]
[2]Dooley M, Plosker GL. Levetiracetam: A review of its adjunctive use in the management of partial onset seizures. Drugs. 2000;60(4):871-93.
[3]Stasiukyniene V, Pilvinis V, Reingardiene D, Janauskaite L. Epileptic seizures in critically ill patients. Medicina (Kaunas). 2009;45(6):501-7. [Lithuanian]
[4]Piri H, Alimohammadi B, Saeedi F, Naderi M, Azhdari Zarmehri H. Anticonvulsant activity of hydro-alcoholicextract of Ziziphoratenuior L. on pentylenetetrazol induced seizure in mice. J Sabzevar Univ Med Sci. 2016;23(1):151-60. [Persian]
[5]Abbasnejad M, Sofiabadi M, Mostafavi A, Kooshki R, Yahyapour M. The effect of ducrosiaanethifolia (Dc.) boissessential oil on hot plate model of pain in adult male rats. J Sabzevar Univ Med Sci. 2014;21(5):761-8. [Persian]
[6]Mahboubi M, Feizabadi MM. Antimicrobial activity of Ducrosia anethifolia essential oil and main component, decanal against methicillin-resistant and methicillin-susceptible Staphylococcus aureus. J Essent Oil Bear Plants. 2009;12(5):574-9.
[7]Haghi G, Safaei A, Safari J. Extraction and determination of the main components of the essential oil of Ducrosia anethifolia by gc and gc/ms. Iran J Pharm Res. 2004;3(Suppl 2):90-7. [Persian]
[8]Stavri M, Mathew K, Bucar F, Gibbons S. Pangelin, an antimycobacterial coumarin from Ducrosia anethifolia. Planta medica.2003;69(10):956-9.
[9]Hajhashemi V, Rabbani M, Ghanadi A, Davari E. Evaluation of antianxiety and sedative effects of essential oil of Ducrosia anethifolia in mice. Clinics. 2010;65(10):1037-42.
[10]Agullo G, Gamet-Payrastre L, Fernandez Y, Anciaux N, Demigné C, Rémésy C. Comparative effects of flavonoids on the growth, viability and metabolism of a colonic adenocarcinoma cell line. Cancer Lett. 1996;105(1):61-70.
[11]Wolfman C, Viola H, Paladini A, Dajas F, Medina JH. Possible anxiolytic effects of chrysin, a central benzodiazepine receptor ligand isolated from Passiflora coerulea. Pharm Biochem Behav. 1994;47(1):1-4.
[12]Zanoli P, Avallone R, Baraldi M. Behavioral characterisation of the flavonoids apigenin and chrysin. Fitoterapia. 2000;7(Suppl 1):117-23.
[13]Ghiasi S, Vaezi GH, Keramati K. Effects of ICV injection of alcoholic extract of Hypericum Perforatum on fear behavior in presence pentylenetetrazole (PTZ) in adult male rat. J Ilam Univ Med Sci. 2010;17(4):36-44. [Persian]
[14]Avallone R, Zanoli P, Corsi L, Cannazza G, Baraldi M. Benzodiazepine-like compounds and GABA in flower heads of Matricaria chamomilla. Phytother Res. 1996;10:177-9.
[15]Duarte FS, Marder M, Hoeller AA, Duzzioni M, Mendes BG, Pizzolatti MG, et al. Anticonvulsant and anxiolytic-like effects of compounds isolated from Polygala sabulosa (Polygalaceae) in rodents: In vitro and in vivo interactions with benzodiazepine binding sites. Psychopharmacology (Berl). 2008;197(3):60-351.
[16]Cadirci E, Suleyman H, Gurbuz P, KruuzumUA, Guvenalp Z, Demirezer L. Anti-inflammatory effects of different extracts from three Salvia species. Turk J Biol. 2011;35:59-64.
[17]Garavand S, Keramati K, Zendehdel M, Jadidoleslami M, Garavand S. Effect of intracerebroventricular injection of flunixin meglumine on PTZ-induced seizures in male rats. Physiol Pharma. 2010;14(1):34-40. [Persian]
[18]Lasoń W, Chlebicka M, Rejdak K. Research advances in basic mechanisms of seizures and antiepileptic drug action.Pharmacol Rep. 2013;65(4):787-801.
[19]Sayyah M, Faraji H, Dehghani A, Bakhtiari H, Kamalinejad M, Narenjkar J. Screening of the anticonvulsant potential of some common medicinal plants of Iran in pentylenetetrazole and maximal electroshock seizure models in male mice. Physiol Pharma. 2011;15(1):66-71. [Persian]
[20]Rebrov I, Karpova M, Andreev A, Kuzina O, Kalinina M, Abrosimov IY, et al. Effect of single injection of pentylenetetrazole in a subconvulsive dose on Cl-conductance of the GABAA-receptor complex. Bull Exp Biol Med. 2004;137(1):6-13.
[21]Werz MA, Macdonald RL. Dynorphin reduces voltage-dependent calcium conductance of mouse dorsal root ganglion neurons. Neuropept. 1984;5(1-3):253-6.
[22]Li K, Xu E. The role and the mechanism of γ-aminobutyric acid during central nervous system development. Neurosci Bull. 2008;24(3):195-200.
[23]Janssen A, Scheffer J, Svendsen AB, Aynehchi Y. The essential oil of Ducrosia anethifolia (DC.) Boiss. Pharm Weekbl. 1984;6(4):60-157.
[24]Chebib M, Johnston GA. GABA-activated ligand gated ion channels: Medicinal chemistry and molecular biology. J Med Chem. 2000;43(8):1427-47.
[25]Jiang JG, Huang XJ, Chen J, Lin QS. Comparison of the sedative and hypnotic effects of flavonoids, saponins, and polysaccharides extracted from Semen Ziziphus jujube. Nat Prod Res. 2007;21(4):20-31.
[26]Karimidokht SA, Oryan S, Parivar K. Anticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice. J Qazvin Univ Med Sci. 2009;13(1):14-20. [Persian]