@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;19(4):236-241
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;19(4):236-241
Protective Effect of Hydroalcoholic Extract of Teucrium polium L. Against Bromobenzene-induced Hepatotoxicity in Mice
ARTICLE INFO
Article Type
Original ResearchAuthors
Kalantari H. (1)Motaharitabar E. (*)
Goudarzi M. (1)
Rashidi M.R. (1)
(*) Pharmacology & Toxicology Department, Pharmacy School, Ahvaz Jundishpur University of Medical Sciences, Ahvaz, Iran
(1) Pharmacology & Toxicology Department, Pharmacy School, Ahvaz Jundishpur University of Medical Sciences, Ahvaz, Iran
Correspondence
Article History
Received: April 16, 2013Accepted: December 26, 2013
ePublished: January 5, 2014
ABSTRACT
Aims
The aim of this study was to investigate protective effect of Teucrium polium L. extract
on bromobenzene-induced hepatotoxicity in mice.
Materials & Methods This experimental study was performed on 48 male albino mice. The animals were randomly divided into six groups. Groups 1 and 2 received normal saline and extract of Teucrium polium (500mg/kg) orally for 10 days, respectively; group 3 received bromobenzene (0.36ml/kg, ip) only on the 10th day; groups 4-6 received ethanolic extract orally in doses of 125, 250 and 500 mg/kg, respectively, during 10 days and bromobenzene (0.36ml/kg, ip) on the 10th day 1 hour after the last dose of extract. Blood and liver samples were collected 24 hours after bromobenzene injection. Then, serum activity of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) and directed and total bilirubin were measured. Finally, the biochemical findings were matched with histopathological verifications, the significance of the differences between groups was compared with ANOVA followed by Tukey’s ad hoc test.
Findings A significant increase in liver enzyme activity by bromobenzene was seen. The treated groups with Teucrium polium showed significant decrease in liver enzyme activity in doses of 125, 250 and 500mg/kg. Histological observations also confirmed the results.
Conclusion Hydroalcoholic extract of Teucrium polium has protective effect on liver toxicity induced by bromobenzene.
Materials & Methods This experimental study was performed on 48 male albino mice. The animals were randomly divided into six groups. Groups 1 and 2 received normal saline and extract of Teucrium polium (500mg/kg) orally for 10 days, respectively; group 3 received bromobenzene (0.36ml/kg, ip) only on the 10th day; groups 4-6 received ethanolic extract orally in doses of 125, 250 and 500 mg/kg, respectively, during 10 days and bromobenzene (0.36ml/kg, ip) on the 10th day 1 hour after the last dose of extract. Blood and liver samples were collected 24 hours after bromobenzene injection. Then, serum activity of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) and directed and total bilirubin were measured. Finally, the biochemical findings were matched with histopathological verifications, the significance of the differences between groups was compared with ANOVA followed by Tukey’s ad hoc test.
Findings A significant increase in liver enzyme activity by bromobenzene was seen. The treated groups with Teucrium polium showed significant decrease in liver enzyme activity in doses of 125, 250 and 500mg/kg. Histological observations also confirmed the results.
Conclusion Hydroalcoholic extract of Teucrium polium has protective effect on liver toxicity induced by bromobenzene.
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