@2024 Afarand., IRAN
ISSN: 1735-7675 Kowsar Medical Journal 2011;16(1):21-25
ISSN: 1735-7675 Kowsar Medical Journal 2011;16(1):21-25
Study of prevalence of Enterotoxin type B gene in Meticillin Resistant Staphylococcus aureus (MRSA) isolated from wound
ARTICLE INFO
Article Type
Original ResearchAuthors
Imani Fouladi A. A. (* )Choupani A. (1 )
Fallah Mehrabadi J. (2 )
(* ) Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
(1 ) Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
(2 ) Asre Novin Bioinformatics Institute, Tehran, Iran
Correspondence
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Fax:
imanifouladi.a@gmail.com
Article History
Received:Accepted:
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ABSTRACT
Aims
Methicillin Resistant Staphylococcus aureus is among common pathogens that its prevalence has increased all around the world during past three decades. Staphylococcal Enterotoxin B (SEB) is one of the most important Staphylococcal enterotoxins, which is considered as an important pathogenic factor. The aim of this study was investigating the correlation between SEB and resistance to Methicillin.
Materials & Methods This cross-sectional study was performed on inpatients of one of the hospitals of Tehran in year 2008 who were selected by accessible sampling method. Correlation between SEB gene in Methicillin Resistant strains was investigated in 100 samples isolated from wounds. Antibiogram was done by disc diffusion and presence of SEB gene was confirmed by PCR. Data was analyzed by SPSS 17 software using descriptive statistical methods and Chi square test.
Results Only 8% of strains had SEB gene. 75% of Staphylococcus aureus strains that had SEB gene were Methicillin resistant and only 15% of them were Methicillin sensitive. A significant correlation was observed between the SEB gene and Methicillin resistance (p<0.05).
Conclusion There is e significant correlation between presence of SEB gene and Methicillin resistance and this issue can reinforce the hypothesis that SEB gene has role in Methicillin resistance.
Materials & Methods This cross-sectional study was performed on inpatients of one of the hospitals of Tehran in year 2008 who were selected by accessible sampling method. Correlation between SEB gene in Methicillin Resistant strains was investigated in 100 samples isolated from wounds. Antibiogram was done by disc diffusion and presence of SEB gene was confirmed by PCR. Data was analyzed by SPSS 17 software using descriptive statistical methods and Chi square test.
Results Only 8% of strains had SEB gene. 75% of Staphylococcus aureus strains that had SEB gene were Methicillin resistant and only 15% of them were Methicillin sensitive. A significant correlation was observed between the SEB gene and Methicillin resistance (p<0.05).
Conclusion There is e significant correlation between presence of SEB gene and Methicillin resistance and this issue can reinforce the hypothesis that SEB gene has role in Methicillin resistance.
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