ARTICLE INFO

Article Type

Original Research

Authors

Tizfahm Tikmehdash   H. (1)
Nasiri Semnani   Sh. (*)
Tajabadi Ebrahimi   M. (2)
Alizadeh   H. (3)
Javadzade   Y. (4)
Hamed Yazdan   S. (5)






(*) Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
(1) Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
(2) Department of Microbiology, Faculty of Sciences, Central Tehran BranchBranch, Islamic Azad University, Tehran, Iran
(3) Young Researchers & Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran
(4) Department of Pharmaceutics, Pharmacy Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
(5) Department of Pharmacognosy, Pharmacy Faculty, Tabriz University of Medical Sciences, Tabriz, Iran

Correspondence

Address: Department of Microbiology, Faculty of Basic Sciences & Medicine, Islamic Azad University, Moallem Street, Zanjan, Iran.
Phone: +982414265890
Fax: +982414265890
sh.nasiri92@yahoo.com

Article History

Received:   December  12, 2011
Accepted:   June 18, 2013
ePublished:   June 25, 2013

ABSTRACT

Aims Gastroenteritis caused by Salmonella typhimurium and the treatment of this disease with antibiotics follows by problems such as drug resistance and side effects emergence. Using herbs and probiotics can be a solution to this problem. The aim of this study was to evaluate the antimicrobial effect of Lactobacillus plantarum with extracts of Satureia hortensis and Anethum geravolens on Salmonella typhimurium; in vitro and in animal model.
Materials & Methods In this experimental study, 35 female mice 6 to 8 weeks old were divided into 7 groups of 5. The mice infected with appropriate strains of Salmonella typhimurium. After extraction of Satureia hortensis and Anethum geravolens, antibacterial effect on Salmonella typhimurium was studied in vitro. Antibacterial activity of 5 strains of Lactobacillus plantarum that showed the highest amount of exopolysacharide production studied on Salmonella typhimurium. Mice were treated with oral and growths of Salmonella typhimurium in their feces were assessed. The results were analyzed by one-way ANOVA test in SPSS 18 software.
Findings Mice which treated with ethanolic extract of Satureia hortensis alone and in combination with probiotic showed significant decrease in the rate of excretion and colonization of Salmonella typhimurium in comparison with mice that treated with ethanolic extract of Anethum geravolens alone and in combination with neutralize probiotic and control groups.
Conclusion Ethanolic extract of Satureia hortensis has a synergistic effect on antimicrobial effect of Lactobacillus plantarum neutralized supernatant against Salmonella typhimurium.


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