@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2013;19(2):89-95
ISSN: 2252-0805 The Horizon of Medical Sciences 2013;19(2):89-95
Synergistic Antimicrobial Effect of Lactobacillus plantarum with Extracts of Satureia hortensis and Anethum geravolens on Salmonella typhimurium; in vitro and in Animal Model
ARTICLE INFO
Article Type
Original ResearchAuthors
Tizfahm Tikmehdash H. (1)Nasiri Semnani Sh. (*)
Tajabadi Ebrahimi M. (2)
Alizadeh H. (3)
Javadzade Y. (4)
Hamed Yazdan S. (5)
(*) Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
(1) Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
(2) Department of Microbiology, Faculty of Sciences, Central Tehran BranchBranch, Islamic Azad University, Tehran, Iran
(3) Young Researchers & Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran
(4) Department of Pharmaceutics, Pharmacy Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
(5) Department of Pharmacognosy, Pharmacy Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
Correspondence
Address: Department of Microbiology, Faculty of Basic Sciences & Medicine, Islamic Azad University, Moallem Street, Zanjan, Iran.Phone: +982414265890
Fax: +982414265890
sh.nasiri92@yahoo.com
Article History
Received: December 12, 2011Accepted: June 18, 2013
ePublished: June 25, 2013
ABSTRACT
Aims
Gastroenteritis caused by Salmonella typhimurium and the treatment of this disease with antibiotics follows by problems such as drug resistance and side effects emergence. Using herbs and probiotics can be a solution to this problem. The aim of this study was to evaluate the antimicrobial effect of Lactobacillus plantarum with extracts of Satureia hortensis and Anethum geravolens on Salmonella typhimurium; in vitro and in animal model.
Materials & Methods In this experimental study, 35 female mice 6 to 8 weeks old were divided into 7 groups of 5. The mice infected with appropriate strains of Salmonella typhimurium. After extraction of Satureia hortensis and Anethum geravolens, antibacterial effect on Salmonella typhimurium was studied in vitro. Antibacterial activity of 5 strains of Lactobacillus plantarum that showed the highest amount of exopolysacharide production studied on Salmonella typhimurium. Mice were treated with oral and growths of Salmonella typhimurium in their feces were assessed. The results were analyzed by one-way ANOVA test in SPSS 18 software.
Findings Mice which treated with ethanolic extract of Satureia hortensis alone and in combination with probiotic showed significant decrease in the rate of excretion and colonization of Salmonella typhimurium in comparison with mice that treated with ethanolic extract of Anethum geravolens alone and in combination with neutralize probiotic and control groups.
Conclusion Ethanolic extract of Satureia hortensis has a synergistic effect on antimicrobial effect of Lactobacillus plantarum neutralized supernatant against Salmonella typhimurium.
Materials & Methods In this experimental study, 35 female mice 6 to 8 weeks old were divided into 7 groups of 5. The mice infected with appropriate strains of Salmonella typhimurium. After extraction of Satureia hortensis and Anethum geravolens, antibacterial effect on Salmonella typhimurium was studied in vitro. Antibacterial activity of 5 strains of Lactobacillus plantarum that showed the highest amount of exopolysacharide production studied on Salmonella typhimurium. Mice were treated with oral and growths of Salmonella typhimurium in their feces were assessed. The results were analyzed by one-way ANOVA test in SPSS 18 software.
Findings Mice which treated with ethanolic extract of Satureia hortensis alone and in combination with probiotic showed significant decrease in the rate of excretion and colonization of Salmonella typhimurium in comparison with mice that treated with ethanolic extract of Anethum geravolens alone and in combination with neutralize probiotic and control groups.
Conclusion Ethanolic extract of Satureia hortensis has a synergistic effect on antimicrobial effect of Lactobacillus plantarum neutralized supernatant against Salmonella typhimurium.
Keywords:
Probiotics,
Salmonella typhimurium,
Lactobacillus plantarum,
Satureia hortensis extract,
Anethum geravolens extract,
CITATION LINKS
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[23] Ogunbanwo ST, Sanni AI, Onilude AA. Characterization of bacteriocin produced by Lactobacillus plantarum Fl and Lactobacillus brevis OGI. Afr J Biotechnol. 2003;2(8):219-27.
[24] Mahboubi M, Haghi G. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. J Ethnopharmacol. 2008;119(2):325-7.
[25] Delaquis PJ, Stanich B, Mazza A, Girard G. Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol. 2002;74(1-2):101-9.
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[27] Coconnier MH, Lievin V, Hemery E, Servin AL. Antagonistic activity against Helicobacter infection in vitro and in vivo by the human Lactobacillus acidophilus strain LB. Appl Environ Microbiol. 1998;64(11):4537-80.
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[29] Ziemer CJ, Gibson GR. An overview of probiotics, prebiotics and synbiotics in the functional food concept: Perspectives and future strategies. J Dairy Sci. 1998;8(5- 6):473-9.
[30] Hagiwara M, Kataoka K, Arimochi H, Kuwahara T, Nakayama H, Ohnishi Y. Inhibitory effect of fluvastatin on ileal ulcer formation in rats induced by nonsteroidal antiinflammatory drug. World J Gastroenterol. 2005;11(7):1040-3.
[31] Chi-Zhang Y, Yam K, Chikindas MS. Effective control of Listeria monocytogenes by combination of nisin formulated and slowlyreleased into a broth system. Int J Food Microbiol. 2004;90(1):15-22.
[32] Tajabady EM, Hejazi MA, Noohi A. Study on probiotic properties of Lactobacilluse isolated from traditional dairy products of Lighvan. Q J Sci Tarbiat Moallem Univ. 2008;7(3):941-52. [Persian]
[33] Hatha AAM. Antimicrobial activity of some of south- Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes, Aeromonas hydrophila. Braz J Microbiol. 2006;37(2):1-2.
[34] Selander RK, Smith NH. Molecular population genetics of Salmonella. Rev Med Microbiol. 1990;1:219-28.
[35] de Roos NK, Katan MB. Effects of probiotic bacteria on diarrhea, lipid metabolism and carcinogenesis: A review of papers published between 1988 and 1998. Am J Clin Nutr. 2000;71(2):405-11.
[36] Cowden JM, Lynch D. Case-control study of infections with Salmonella enteritidis phage type 4 in England. Br Med J. 1989;299:771-3.
[37] Heyman M, Menard S. Probiotic microorganism: How they affect intestinal pathophysiology. Cell Mol Life Sci. 2002;59:1151-65.
[38] Lefteris M, Luc DV. The in vitro inhibition of Gramnegative pathogenic bacteria by Bifidobacteria is caused by the production of organic acid. Int Dairy J. 2004;16(9):1049- 57.
[39] Bodana AR, Rao DR. Antimutagenic activity of milk fermented by Strptococcus thermophilus and Lactobacillus. J Dairy Sci. 1990;73:3379-84.
[40] Akalin As, Gonc S, Duzel S. Influence of yogurt and acidophilus yogurt on serum cholesterol levels in mice. J Dairy Sci. 1997;80(11):2721-5.
[41] Pol IE, Krommer J, Smid EJ. Bioenergetics consequences of nisin combined with caracole towards Bacillus cereus. Innov Food Sci Emerg Technol. 2002;3(1):55-61.
[42] Khanafari A, Hosseini F. Practical microbiology and biochemical principles of reactions. Tehran: Poorsina Publication; 2009. [Persian]
[43] Lefteris M, Luc DV. The in vivo inhibition of Gramnegative pathogenic bacteria by Bifidobacteria is caused by the production of organic acid. Int Dairy J. 2006;16(9):1049- 57.
[2] Ray SM, Ahuga SD. Population, based, surveillance for yersinia entrocolitica infections in food net-sites, 1996- 1999: Higher risk of disease in infants and minority populations. Clin Infect Dis. 2004;38(l3):5181.
[3] Cetinkaya F, Cibik R, Soyutemiz GE, Ozakin C, Kayali R, Levent B. Shigella and Salmonella contamination in various foodstuffs in Turkey. Food Control. 2007;19(11):1059-63.
[4] Weiss SH, Blaser MJ. Occurrence and distribution of serotypes of the Arizona subgroup of Salmonella strain in the United States from 1967 to 1976. J Clin Microbiol. 1986;23(6):1056-64.
[5] Schrezenmeir J, de Vrese M. Probiotics, prebiotics and synbiotics approaching a definition. Am J Clin Nutr. 2001;73(2):361-4.
[6] Oyetayo VO, Oyetayo FL. Review potential of probiotics as biotherapeutic agents targeting the innate immune system. Afr J Biotechnol. 2005;4(2):123-7.
[7] Kandler O. Bergey's manual of systematic bacteriology. Philadelphia, PA: Lippincott Williams and Wilkins; 1989.
[8] Rafter J. The effects of probiotics on colon cancer development. Nutr Res Rev. 2004;17(2):277-84.
[9] Perdigon G, Alvarez S, Rachid M, Aguero G, Gobbato N. Immune system stimulation by probiotics. J Dairy Sci. 1995;78(7):1597-606.
[10] Parves S, Malik KA, Kang A, Kim HY. Probiotic and fermented food products are beneficial for health. J Appl Microbiol. 2006;100(6):1171-85.
[11] Lomax AR, Calder PC. Probiotics, immune function, infection and inflammation: A review of the evidence from studies conducted in humans. Curr Pharm Des. 2009;15(11);1428-518.
[12] Kukkonen K, Savilahti E, Haahtela T, Juntunen- Backman K, Korpela, R, Poussa T. Probiotics and prebiotic galacto-oligosaccharides in the prevention of allergic diseases: A randomized, double-blind, placebo-controlled trial. J Allergy Clin Immunol. 2007;119(1):192-8.
[13] Raymond R. Adipic acid: Handbook of pharmaceutical. Excipients. 2009;1:11-2.
[14] Ertesvag H, Valla S. Biosynthesis and applications of alginates. Polym Degrod Stabil. 1998;59:85-91.
[15] Oyetayo VO, Oyetayo, FL. Potential of probiotics as biotherapeutic agents targeting the innate immune system. African J Biotech. 2005;4(2):123-7.
[16] Collins JK, Thornton G, Sullivan GO. Selection of probiotic strains for human applications. Int Dairy J. 1998;8:487-90.
[17] Fooks LJ, Gibson GR. Probiotics as modulators of the gut flora. Br J Nutr. 2002;88(1):39-49.
[18] Schuenzel KM, Harrison MA. Microbial antagonists of food borne pathogens on fresh, minimally processed vegetables. J Food Protect. 2002;65(12):1909-15.
[19] Sefidkon F, Jamzad Z, Barazandeh M. Essential oil of Satureja bachtiarica Bunge: A potential source of carvacrol. Iran J Med Aromat Plant. 2005;20(4):425-39. [Persian]
[20] Abbasi KH, Sefidkon F, Yamini Y. Comparison of oil content and composition of two Satureja species (Satureja hortensis L. and Satureja rechingeri Jamzad) by hydrodistillation and supercritical fluid extraction. Iran J Med Aromat Plant. 2005;21(3):307-18. [Persian]
[21] Singh G, Maurya S, Catalan C. Chemical constituent’s antimicrobial investigations and antioxidant potentials of Anethum graveolens essential oil and acetone extract. J Food Sci. 2005;70(4):208-15.
[22] Misaghi A, Basti AA. Effect of Zataria multiflora boiss: Essential oil and nisin on Bacillus cereus ATCC 11778. Food Control. 2007;1(9):1043-9.
[23] Ogunbanwo ST, Sanni AI, Onilude AA. Characterization of bacteriocin produced by Lactobacillus plantarum Fl and Lactobacillus brevis OGI. Afr J Biotechnol. 2003;2(8):219-27.
[24] Mahboubi M, Haghi G. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. J Ethnopharmacol. 2008;119(2):325-7.
[25] Delaquis PJ, Stanich B, Mazza A, Girard G. Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol. 2002;74(1-2):101-9.
[26] Bahramikia S, Yazdanparast R. Antioxidant and free radical scavenging activities of different fractions of Anethum graveolens leaves using in vitro models. Pharmacol Online. 2008;2:233-19.
[27] Coconnier MH, Lievin V, Hemery E, Servin AL. Antagonistic activity against Helicobacter infection in vitro and in vivo by the human Lactobacillus acidophilus strain LB. Appl Environ Microbiol. 1998;64(11):4537-80.
[28] Young-Hyo C, Jong-Keun K, Hong-Joong K, Won- Yong K, Young-Bae K, Yong-Ha P. Selection of a potential probiotic Lactobacillus strain and subsequent in vivo studies. Antonie van Leeuwenhoek. 2001;80:193-9.
[29] Ziemer CJ, Gibson GR. An overview of probiotics, prebiotics and synbiotics in the functional food concept: Perspectives and future strategies. J Dairy Sci. 1998;8(5- 6):473-9.
[30] Hagiwara M, Kataoka K, Arimochi H, Kuwahara T, Nakayama H, Ohnishi Y. Inhibitory effect of fluvastatin on ileal ulcer formation in rats induced by nonsteroidal antiinflammatory drug. World J Gastroenterol. 2005;11(7):1040-3.
[31] Chi-Zhang Y, Yam K, Chikindas MS. Effective control of Listeria monocytogenes by combination of nisin formulated and slowlyreleased into a broth system. Int J Food Microbiol. 2004;90(1):15-22.
[32] Tajabady EM, Hejazi MA, Noohi A. Study on probiotic properties of Lactobacilluse isolated from traditional dairy products of Lighvan. Q J Sci Tarbiat Moallem Univ. 2008;7(3):941-52. [Persian]
[33] Hatha AAM. Antimicrobial activity of some of south- Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes, Aeromonas hydrophila. Braz J Microbiol. 2006;37(2):1-2.
[34] Selander RK, Smith NH. Molecular population genetics of Salmonella. Rev Med Microbiol. 1990;1:219-28.
[35] de Roos NK, Katan MB. Effects of probiotic bacteria on diarrhea, lipid metabolism and carcinogenesis: A review of papers published between 1988 and 1998. Am J Clin Nutr. 2000;71(2):405-11.
[36] Cowden JM, Lynch D. Case-control study of infections with Salmonella enteritidis phage type 4 in England. Br Med J. 1989;299:771-3.
[37] Heyman M, Menard S. Probiotic microorganism: How they affect intestinal pathophysiology. Cell Mol Life Sci. 2002;59:1151-65.
[38] Lefteris M, Luc DV. The in vitro inhibition of Gramnegative pathogenic bacteria by Bifidobacteria is caused by the production of organic acid. Int Dairy J. 2004;16(9):1049- 57.
[39] Bodana AR, Rao DR. Antimutagenic activity of milk fermented by Strptococcus thermophilus and Lactobacillus. J Dairy Sci. 1990;73:3379-84.
[40] Akalin As, Gonc S, Duzel S. Influence of yogurt and acidophilus yogurt on serum cholesterol levels in mice. J Dairy Sci. 1997;80(11):2721-5.
[41] Pol IE, Krommer J, Smid EJ. Bioenergetics consequences of nisin combined with caracole towards Bacillus cereus. Innov Food Sci Emerg Technol. 2002;3(1):55-61.
[42] Khanafari A, Hosseini F. Practical microbiology and biochemical principles of reactions. Tehran: Poorsina Publication; 2009. [Persian]
[43] Lefteris M, Luc DV. The in vivo inhibition of Gramnegative pathogenic bacteria by Bifidobacteria is caused by the production of organic acid. Int Dairy J. 2006;16(9):1049- 57.