@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(2):121-128
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(2):121-128
Analgesic and Anti-Inflammatory Effects of Hydroalcoholic Extract of Salvia multicaulis on Male Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Abdolmaleki A. (1)Fereidoni M. (*)
Farhadi Moghadam B. (1)
Asgari A. (2)
(*) Biology Department, Sciences Faculty, Ferdowsi University of Mashhad, Mashhad, Iran
(1) Biology Department, Sciences Faculty, Ferdowsi University of Mashhad, Mashhad, Iran
(2) Agronomy Department, Agriculture Faculty, Ferdowsi University of Mashhad, Mashhad, Iran
Correspondence
Address: Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Vakilabad Highway, Mashhad, Iran. Postal Code: 9177948974Phone: +985136214026
Fax: +985138762227
fereidoni@um.ac.ir
Article History
Received: January 18, 2015Accepted: May 3, 2015
ePublished: June 20, 2015
ABSTRACT
Aims
One of the most basic methods for coping with diseases and pain relief had been the use of medicinal plants. The aim of this study was to the determination of analgesic and anti-inflammatory effects of Salvia multicaulis hydroalcoholic extract.
Materials & Methods In this experimental study, 42 male Wistar rats were divided in 6 groups (n=7); the control (receive nothing), the sham (receive solvent intraperitoneally) and 4 groups that received plant extract at the doses of 50, 100, 200 and 400mg/kg intraperitoneally. Tail flick and formalin test were used for evaluation of thermal and chemical pain, also for assessment the degree of inflammation, rat paw edema volume was acquired by plethysmometric test. Data were analyzed in SPSS 16 software by ANOVA with repeated measuring and T student tests.
Findings The intraperitoneally injection of extract in all doses decreased the chemical pain induced by formalin (p<0.05). Hydroalcoholic extract of plant at doses 200 and 400mg/kg caused hyperalgesia in compared with control group (p<0.01). All concentrations of hydroalcoholic extract of plant decreased the inflammation (p<0.01).
Conclusion Intraperitoneally administration of hydroalcoholic extract of Salvia multicaulis has analgesic effects.
Materials & Methods In this experimental study, 42 male Wistar rats were divided in 6 groups (n=7); the control (receive nothing), the sham (receive solvent intraperitoneally) and 4 groups that received plant extract at the doses of 50, 100, 200 and 400mg/kg intraperitoneally. Tail flick and formalin test were used for evaluation of thermal and chemical pain, also for assessment the degree of inflammation, rat paw edema volume was acquired by plethysmometric test. Data were analyzed in SPSS 16 software by ANOVA with repeated measuring and T student tests.
Findings The intraperitoneally injection of extract in all doses decreased the chemical pain induced by formalin (p<0.05). Hydroalcoholic extract of plant at doses 200 and 400mg/kg caused hyperalgesia in compared with control group (p<0.01). All concentrations of hydroalcoholic extract of plant decreased the inflammation (p<0.01).
Conclusion Intraperitoneally administration of hydroalcoholic extract of Salvia multicaulis has analgesic effects.
CITATION LINKS
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[24]Verdi J, Kamalinezhad M, Sabet Kasaei M, Sharif SH. Analgesic Effect Of Aqueous Satureja Hortensis L. Seed Extract In Male Rat. Physiol Pharmacol J. 2004;8(2):163-8. [Persian]
[25]Vaez G, Tavasoli Z, Ranjbar Bahadori S. Study on the different dosages of Elaeagnus angustifolia aqueous extract with and without morphine on the antinociceptive rate in mice. Pejouhesh. 2011;35(1):27-33. [Persian]
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[27]Parveen Z, Yulin D, Muhammad KS, Rongji D, Waqar A, Yu Hong Y. Antiinflammatory and analgesic activities of Thesium chinense Turcz extracts and its major flavonoids, kaempferol and kaempferol-3-O-glucoside. Yakugaku Zasshi. 2007;127(8):1275-9.
[28]Hoodgar F, Nasri S, Amin GHR. Investigation of antinociceptive and anti-inflammatory effects of hydro-alcoholic extract of Securigera securidaca L. Horizon Med Sci. 2011;17(2):12-9. [Persian]
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[31]Morshedi A, Dashti MH, Dehghan HM, Bagherinasab MA, Salami AS. The effect of Artemisia sieberi Besser on inflammatory and neurogenic pain in mice. JMP. 2011;4(40):48-57. [Persian]
[32]Nagy I, Rang H. Noxious heat activates all capsaicin-sensitive and also a sub-population of capsaicin-insensitive dorsal root ganglion neurons. Neuroscience. 1999;88(4):995-7.
[33]Julius D, Basbaum AI. Molecular mechanisms of nociception. Nature. 2001;413(6852):203-10.
[34]Pahlavan Y, Sepehri GR, Afarinesh Khaki MR, Sheibani V, Esmail Pour K, Pahlavan B. Intervention of morphine and naloxone on analgesic effects of origanum vulgare extract in male rat. J Ardabil Univ Med Sci. 2011;11(2):134-42. [Persian]
[2]Bahmani M, Shirzad H, Majlesi M, Shahinfard N, Rafieian Kopaei M. A review study on analgesic applications of Iranian medicinal plants. Asian Pac J Trop Med. 2014;7S1:S43-53.
[3]De Melo MS, Quintans JJS, Araújo AAS, Duarte MC, Bonjardim LR, Nogueira PCL, et al. A systematic review for anti-inflammatory property of clusiaceae family: A preclinical approach. Evid Based Complement Alternat Med. 2014;960258.
[4]Cash JL, Norling LV, Perretti M. Resolution of inflammation: Targeting GPCRs that interact with lipids and peptides. Drug Discov Today. 2014;19(8):1186-92.
[5]Nishimoto N, Kishimoto T. Interleukin 6: From bench to bedside. Nat Clin Pract Rheumatol. 2006;2(11):619-26.
[6]Saeed MK, Deng Y, Dai R, Li W, Yu Y, Iqbal Z. Appraisal of antinociceptive and anti-inflammatory potential of extract and fractions from the leaves of Torreya grandis Fort Ex. J Ethnopharmacol. 2010;127(2):414-8.
[7]Amiri H. Chemical composition and antioxidant activity of the essential oil and methanolic extract of Salvia multicaulis Vahl. JMP. 2012;1(41):111-7. [Persian]
[8]Eidi A, Parivar K, Mazouji A, Akhtari Z. Antinociceptive effects of essential oil of Salvia hypoleuca L. in mice. Med Sci J Islamic Azad Univ. 2006;16(3):165-9. [Persian]
[9]Azizi A, Azizi AM, Azizi G. Essential oils composition and antimicrobal effects of essential oils and methanol extracts of Salvia multicaulis. Vahl against Xanthomonas translucens pv. Cerealis. J Plant Sci Res. 2009;15(3):39-48.
[10]Carta C, Morettib DLM, Peana AT. Activity of the oil of Salvia officinalis L. against Botrytis cinerea. J Essen Oil Res. 1996;8(4):399-404.
[11]Piccaglia R, Marotti M, Dellacecca V. Effect of planting density and harvest date on yield and chemical composition of sage oil. J Essen Oil Res. 1997;9(2):187-91.
[12]Mancini E, Arnold NA, De Martino L, De Feo V, Formisano C, Rigano D, et al. Chemical composition and phytotoxic effects of essential oils of Salvia hierosolymitana boiss. and Salvia multicaulis vahl. var. Simplicifolia boiss. growing wild in Lebanon. Molecules. 2009;14(11):4725-36.
[13]Ulubelen A, Topcu G. Salvimultine, a new noricetexane diterpene from the roots of Salvia multicaulis. J Nat Prod. 2000;63(6):879-80.
[14]Şahin F, Güllüce M, Daferera D, Sökmen A, Sökmen M, Polissiou M, et al. Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of Turkey. Food Control. 2004;15(7):549-57.
[15]Adams RP. Identification of essential oil components by gas chromatography/mass spectroscopy. J Am Soc Mass Spectrom. 1997;6(8):671-2.
[16]Zimmermann M. Ethical considerations in relation to pain in animal experimentation. Acta Physiol Scand Suppl. 1986;554:221-33.
[17]Dubuisson D, Dennis SG. The formalin test: A quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats. Pain. 1977;4(2):161-74.
[18]Heidari Oranjaghi N, Azhdari Zarmehri H, Erami E, Haghparast A. Antagonism of orexin-1 receptors attenuates swim-and restraint stress-induced antinociceptive behaviors in formalin test. Pharmacol Biochem Behav. 2012;103(2):299-307.
[19]Randolph BC, Peters MA. Analgesic effectiveness of ketorolac compared to meperidine in the rat formalin test. Anesth Prog. 1997;44(1):11-6.
[20]D'amour FE, Smith DL. A method for determining loss of pain sensation. J Pharmacol Exp Ther. 1941;72(1):74-9.
[21]Fereidoni M, Ahmadiani A, Semnanian S, Javan M. An accurate and simple method for measurement of paw edema. J Pharmacol Toxicol Methods. 2000;43(1):11-4.
[22]Tepe B, Donmez E, Unlu M, Candan F, Daferera D, Vardar-Unlu G, et al. Antimicrobial and antioxidative activities of the essential oils and methanol extracts of Salvia cryptantha (Montbret et Aucher ex Benth.) and Salvia multicaulis (Vahl). Food Chem. 2004;84(4):519-25.
[23]Heidari MR, Vahedian M, Moamenzadeh S, Hayatbakhsh Abbasi MM. The analgesic effect and possible mechanism of colchicum szovitsii methanolic extract in mouse. J Rafsanjan Univ Med Sci. 2005;4(1):25-33. [Persian]
[24]Verdi J, Kamalinezhad M, Sabet Kasaei M, Sharif SH. Analgesic Effect Of Aqueous Satureja Hortensis L. Seed Extract In Male Rat. Physiol Pharmacol J. 2004;8(2):163-8. [Persian]
[25]Vaez G, Tavasoli Z, Ranjbar Bahadori S. Study on the different dosages of Elaeagnus angustifolia aqueous extract with and without morphine on the antinociceptive rate in mice. Pejouhesh. 2011;35(1):27-33. [Persian]
[26]Taherian AA, Etemadi H, Sadeghi H. Assessment of aqueous extract of seed of Cuminum cyminum L. on neurogenic and inflammatory pain in mice. JMP. 2007;4(24):44-50. [Persian]
[27]Parveen Z, Yulin D, Muhammad KS, Rongji D, Waqar A, Yu Hong Y. Antiinflammatory and analgesic activities of Thesium chinense Turcz extracts and its major flavonoids, kaempferol and kaempferol-3-O-glucoside. Yakugaku Zasshi. 2007;127(8):1275-9.
[28]Hoodgar F, Nasri S, Amin GHR. Investigation of antinociceptive and anti-inflammatory effects of hydro-alcoholic extract of Securigera securidaca L. Horizon Med Sci. 2011;17(2):12-9. [Persian]
[29]Alibabaei Z, Pilehvarian AA, Shirani M, Kheiri S, Taji F, Asgari A, et al. Effect of Euphorbia helioscopia on acetic acid-induced abdominal constrictions in Balb/c mice. J Shahrekord Univ Med Sci. 2010;11(4):9-14. [Persian]
[30]Dashti Rahmatabadi MH, Vahidi Mehrjardi AR, Pilavaran AA, Farzan F. Antinociceptive effect of cinnamon extract on formalin induced pain in rat. JSSU. 2009;17(2):190-9. [Persian]
[31]Morshedi A, Dashti MH, Dehghan HM, Bagherinasab MA, Salami AS. The effect of Artemisia sieberi Besser on inflammatory and neurogenic pain in mice. JMP. 2011;4(40):48-57. [Persian]
[32]Nagy I, Rang H. Noxious heat activates all capsaicin-sensitive and also a sub-population of capsaicin-insensitive dorsal root ganglion neurons. Neuroscience. 1999;88(4):995-7.
[33]Julius D, Basbaum AI. Molecular mechanisms of nociception. Nature. 2001;413(6852):203-10.
[34]Pahlavan Y, Sepehri GR, Afarinesh Khaki MR, Sheibani V, Esmail Pour K, Pahlavan B. Intervention of morphine and naloxone on analgesic effects of origanum vulgare extract in male rat. J Ardabil Univ Med Sci. 2011;11(2):134-42. [Persian]