@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(3):139-143
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(3):139-143
Effect of Palm Pollen Extract on Sexual Hormone Levels and Follicle Numbers in Adult Female BALB/c mice
ARTICLE INFO
Article Type
Original ResearchAuthors
Hosseini S.E. (* )Mehrabani D. (1 )
Razavi F. (2 )
(* ) Biology Department, Sciences Faculty, Fars Science & Research Branch, Islamic Azad University, Shiraz, Iran
(1 ) Pathology Department, Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
(2 ) Biology Department, Sciences Faculty, Fars Science & Research Branch, Islamic Azad University, Shiraz, Iran
Correspondence
Address: Biology Department, Sciences Faculty, Islamic Azad University, Kilometer 18th of Marvdasht- Sadd-e-Dorudzan Road, IranPhone: +987143311148
Fax: +987143311172
ebrahim.hossini@yahoo.com
Article History
Received: October 4, 2013Accepted: July 7, 2014
ePublished: September 23, 2014
ABSTRACT
Aims
Using chemical drugs to treat disease often is associated with side effects. Recently,
using herbal medicines, which have fewer side effects, has flourished to treat many diseases,
including infertility. This study was done aimed to investigate the effect of palm pollen
aqueous extract on sexual hormones and follicle cells numbers in adult female BALB/c mice.
Materials & Methods This experimental study was done in animal house of Shiraz University of Medical Sciences in 2012. 40 adult female BALB/c mice were divided into 5 control, sham and experimental groups receiving 100, 200 and 400mg/kg of the body weight of the extract groups each with 8 members. Levels of testosterone, estrogen and progesterone were measured by radioimmunoassay and ovarian follicles numbers were counted after separation of the ovaries and sectioning and staining using Hematoxylin & Eosin by light microscopy. The results of hormonal assays were analyzed based on One-way ANOVA and Duncan’s multiple range tests using SPSS 18 software.
Findings A significant increase was observed at the level of testosterone, estrogen and progesterone compared to the control group only in the experimental group 3 (receiving 400mg/kg palm pollen extract) (p<0.01). Also, only in the experimental group 3 a significant increase was observed in the number of secondary follicles (p<0.05) and the number of antral follicles (p<0.01) compared to the control.
Conclusion 400mg/kg palm pollen aqueous extract increases the amount of sexual hormones and secondary and antral follicle numbers in adult female mice.
Materials & Methods This experimental study was done in animal house of Shiraz University of Medical Sciences in 2012. 40 adult female BALB/c mice were divided into 5 control, sham and experimental groups receiving 100, 200 and 400mg/kg of the body weight of the extract groups each with 8 members. Levels of testosterone, estrogen and progesterone were measured by radioimmunoassay and ovarian follicles numbers were counted after separation of the ovaries and sectioning and staining using Hematoxylin & Eosin by light microscopy. The results of hormonal assays were analyzed based on One-way ANOVA and Duncan’s multiple range tests using SPSS 18 software.
Findings A significant increase was observed at the level of testosterone, estrogen and progesterone compared to the control group only in the experimental group 3 (receiving 400mg/kg palm pollen extract) (p<0.01). Also, only in the experimental group 3 a significant increase was observed in the number of secondary follicles (p<0.05) and the number of antral follicles (p<0.01) compared to the control.
Conclusion 400mg/kg palm pollen aqueous extract increases the amount of sexual hormones and secondary and antral follicle numbers in adult female mice.
CITATION LINKS
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[2]Massaro D, Massaro GD. Estrogen regulates pulmonary alveolar formation, loss, and regeneration in mice. Am J Physiol Lung Cell Mol Physiol. 2004;287(6):1154-9.
[3]Grishkovskaya I, Avvakumov GV, Hammond GL, Muller YA. Resolution of a disordered region at the entrance of the human sex hormone-binding globulin steroid-binding site. J Mol Biol. 2002;318(3):621-6.
[4]Biglari F, AlKarkhi AF, Easa AM. Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran. Food Chem. 2008;107(4):1636-41.
[5]Al-Taher AY. Possible anti-diarrhoeal effect of the date palm (Phoenix Dactylifera L.) spathe aqueous extract in Rats. Sci J King Fasial Univ Basic Appl Sci. 2008;9:121-37.
[6]Hosseini SE, Mehrabani D, Razavi FS, RafieiRad M. Effect of palm pollen aqueous extract on the sex ratio of offspring in mice strain BALB/c. Q Res J Lorestan Uni Med Sci. 2013;15(2):121-8. [Persian]
[7]Barh D, Mazumdar BC. Comparative nutritive values of palm saps before and after their partial fermentation and effective use of wild date (Phoenix sylvestris Roxb.) sap in treatment of anemia. Res J Med Sci. 2008;3(2):173-6.
[8]Bahmanpour S, Talaei T, Vojdani Z, Panjehshahin MR, Poostpasand A, Zareei S, et al. Effect of Phoenix Dactylifera pollen on sperm parameters and reproductive system of adult male rats. IJMS. 2006;31(4):208-12.
[9]Burgoyne RW, Tan DH. Prolongation and quality of life for HIV-infected adults treated with highly active antiretroviral therapy (HAART): A balancing act. J Antimicrob Chemother. 2008;61(3):469-73.
[10]Jassim SA, Naji MA. In vitro evaluation of the antibacterial activity of an extract of date palm (Pheonix dactylifera L.) pits on a pseudomonas phage. Evid Based Complement Alternat Med. 2010;7(1)57-62.
[11]Al-Qarawi AA, Mousa HM, Ali BH, Abdel-Rahman H, El-Mougy SA. Protective effect of extracts from dates (Phoenix dactylifera L.) on carbon tetrachloride-induced hepatotoxicity in rats. Intern J Appl Res Vet Med. 2004;2(3):176-80.
[12]Abedi A, Parviz M, Karimian SM, Sadeghipour Rodsari HR. The effect of aqueous extract of Pheonix dactylifera pollen grain on sexual behavior of male rats. J Physiol Pharmacol Adv. 2012;2(6):235-42.
[13]Padashetty SA, Mishra SH. Aphrodisiac studies of Trichlepis glaberrima with supportive action from antioxidant enzymes. Pharm Biol. 2007;45(7):580-6.
[14]Tivis LJ, Richardson MD, Peddi E, Arjmandi B. Saliva versus serum estradiol: implications for research studies using postmenopausal women. Prog Neuropsychopharmacol Biol Psychiat. 2005;29(5):727-32.
[15]Moshfeghi M, Hosseini SE, Vahdati A, Moshfegh Z. The Study of the Effects of Dexamethasone During Pregnancy on development and Function of Ovary Tissue in Offspring of First Lineage Rats. Q J Anim Physiol Dev. 2014;7(2):23-30.
[16]Yakuba MT, Akanji MA, Oladiji AT, Adesokan AA. Androgenic potential of aqueous extract of Massularia acuminata (G. Don) Bullock ex Hoyl. Stem in male Wistar rats. J Ethnopharmacol. 2008;118(3):508-13.
[17]Jahnson J, Canning J, Kaneko T, Pru JK, Tilly JL. Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature. 2004;428(6979):145-50.
[18]Moshtaghi A, Johari H, Shariati M, Amiri J. Effects of Phoenix dactylifera on serum concentration of estrogen, progesterone and gonadotropins in adult female rats. J Rafsanjan Uni Med Sci. 2010;9(2):117-24. [Persian]
[19]Shariati M, Sharifi E, Kaveh M. The effect of phoenix dactylifera (date-palm) pit powder on testosterone level and germ cell in adult male rats. Sci J Zanjan Uni Med Sci. 2007;15(61):21-8. [Persian]
[20]Adimoelja A. Phytochemicals and the breakthrough of traditional herbs in the management of sexual dysfunctions. Int J Androl. 2000;23(Suppl 2):82-4.
[21]Abbas FA, Ateya AM. Estradiol, esteriol, estrone and novel flavonoids from date palm pollen. Aust J Basic Appl Sci. 2011;5(8):606-14.
[22]El Mougy SA, Abed Aziz SA, Al-Shanawany M, Omar A. The gonadotropic activity of palmae in mature male rats. Alexandria J Pharm Sci. 1991;5:156-9.
[23]Shariati M, Sharifi E, Kaveh M. The effect of Phoenix Dactylifera (Date-palm) pit powder on testosterone level and germ cells in adult male rats. ZUMS. 2007;15(61):21-8.
[24]Familiari G, Heyn R, Relucenti M, Nottola SA, Sathananthan AH. Ultrastructural dynamics of human reproduction, from ovulation to fertilization and early embryo development. Int Rev Cytol. 2006;249:53-141.
[2]Massaro D, Massaro GD. Estrogen regulates pulmonary alveolar formation, loss, and regeneration in mice. Am J Physiol Lung Cell Mol Physiol. 2004;287(6):1154-9.
[3]Grishkovskaya I, Avvakumov GV, Hammond GL, Muller YA. Resolution of a disordered region at the entrance of the human sex hormone-binding globulin steroid-binding site. J Mol Biol. 2002;318(3):621-6.
[4]Biglari F, AlKarkhi AF, Easa AM. Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran. Food Chem. 2008;107(4):1636-41.
[5]Al-Taher AY. Possible anti-diarrhoeal effect of the date palm (Phoenix Dactylifera L.) spathe aqueous extract in Rats. Sci J King Fasial Univ Basic Appl Sci. 2008;9:121-37.
[6]Hosseini SE, Mehrabani D, Razavi FS, RafieiRad M. Effect of palm pollen aqueous extract on the sex ratio of offspring in mice strain BALB/c. Q Res J Lorestan Uni Med Sci. 2013;15(2):121-8. [Persian]
[7]Barh D, Mazumdar BC. Comparative nutritive values of palm saps before and after their partial fermentation and effective use of wild date (Phoenix sylvestris Roxb.) sap in treatment of anemia. Res J Med Sci. 2008;3(2):173-6.
[8]Bahmanpour S, Talaei T, Vojdani Z, Panjehshahin MR, Poostpasand A, Zareei S, et al. Effect of Phoenix Dactylifera pollen on sperm parameters and reproductive system of adult male rats. IJMS. 2006;31(4):208-12.
[9]Burgoyne RW, Tan DH. Prolongation and quality of life for HIV-infected adults treated with highly active antiretroviral therapy (HAART): A balancing act. J Antimicrob Chemother. 2008;61(3):469-73.
[10]Jassim SA, Naji MA. In vitro evaluation of the antibacterial activity of an extract of date palm (Pheonix dactylifera L.) pits on a pseudomonas phage. Evid Based Complement Alternat Med. 2010;7(1)57-62.
[11]Al-Qarawi AA, Mousa HM, Ali BH, Abdel-Rahman H, El-Mougy SA. Protective effect of extracts from dates (Phoenix dactylifera L.) on carbon tetrachloride-induced hepatotoxicity in rats. Intern J Appl Res Vet Med. 2004;2(3):176-80.
[12]Abedi A, Parviz M, Karimian SM, Sadeghipour Rodsari HR. The effect of aqueous extract of Pheonix dactylifera pollen grain on sexual behavior of male rats. J Physiol Pharmacol Adv. 2012;2(6):235-42.
[13]Padashetty SA, Mishra SH. Aphrodisiac studies of Trichlepis glaberrima with supportive action from antioxidant enzymes. Pharm Biol. 2007;45(7):580-6.
[14]Tivis LJ, Richardson MD, Peddi E, Arjmandi B. Saliva versus serum estradiol: implications for research studies using postmenopausal women. Prog Neuropsychopharmacol Biol Psychiat. 2005;29(5):727-32.
[15]Moshfeghi M, Hosseini SE, Vahdati A, Moshfegh Z. The Study of the Effects of Dexamethasone During Pregnancy on development and Function of Ovary Tissue in Offspring of First Lineage Rats. Q J Anim Physiol Dev. 2014;7(2):23-30.
[16]Yakuba MT, Akanji MA, Oladiji AT, Adesokan AA. Androgenic potential of aqueous extract of Massularia acuminata (G. Don) Bullock ex Hoyl. Stem in male Wistar rats. J Ethnopharmacol. 2008;118(3):508-13.
[17]Jahnson J, Canning J, Kaneko T, Pru JK, Tilly JL. Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature. 2004;428(6979):145-50.
[18]Moshtaghi A, Johari H, Shariati M, Amiri J. Effects of Phoenix dactylifera on serum concentration of estrogen, progesterone and gonadotropins in adult female rats. J Rafsanjan Uni Med Sci. 2010;9(2):117-24. [Persian]
[19]Shariati M, Sharifi E, Kaveh M. The effect of phoenix dactylifera (date-palm) pit powder on testosterone level and germ cell in adult male rats. Sci J Zanjan Uni Med Sci. 2007;15(61):21-8. [Persian]
[20]Adimoelja A. Phytochemicals and the breakthrough of traditional herbs in the management of sexual dysfunctions. Int J Androl. 2000;23(Suppl 2):82-4.
[21]Abbas FA, Ateya AM. Estradiol, esteriol, estrone and novel flavonoids from date palm pollen. Aust J Basic Appl Sci. 2011;5(8):606-14.
[22]El Mougy SA, Abed Aziz SA, Al-Shanawany M, Omar A. The gonadotropic activity of palmae in mature male rats. Alexandria J Pharm Sci. 1991;5:156-9.
[23]Shariati M, Sharifi E, Kaveh M. The effect of Phoenix Dactylifera (Date-palm) pit powder on testosterone level and germ cells in adult male rats. ZUMS. 2007;15(61):21-8.
[24]Familiari G, Heyn R, Relucenti M, Nottola SA, Sathananthan AH. Ultrastructural dynamics of human reproduction, from ovulation to fertilization and early embryo development. Int Rev Cytol. 2006;249:53-141.