@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(3):165-170
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(3):165-170
Immunomodulatory Effects of 17 beta-Estradiol in NMRI Mice Immunized by SRBC
ARTICLE INFO
Article Type
Original ResearchAuthors
Abtahi Froushani S.A. (* )Esmaeili Gouvarchin Ghaleh H. (1 )
Mansouri Mothlagh B. (1 )
Babaei M. (1 )
(* ) Microbiology Department, Veterinary Faculty, Urmia University, Urmia, Iran
(1 ) Microbiology Department, Veterinary Faculty, Urmia University, Urmia, Iran
Correspondence
Address: Microbiology Department, Veterinary Faculty, Nazlu Pardis, Kilometer 11th of Sarv Road, Urmia, IranPhone: +984432770508
Fax: +984432771926
meysamabtahi@hotmail.com
Article History
Received: December 17, 2013Accepted: August 16, 2014
ePublished: September 23, 2014
ABSTRACT
Aims
Steroid hormones have powerful effect on all levels of the innate and adaptive immune
systems. Among steroid hormones, estrogens play a key role in the regulation of homeostasis
of bone marrow activity, cardiovascular system and central nervous system. The main
purpose of this study was to determine the possible effects of moderating role of estrogen
on humoral and cellular immune responses following the sheep red blood cells challenge in
the mouse model.
Materials & Methods 14 mice were divided into two groups and were immunized with SRBC antigen. The treated mice were injected with estrogen for two weeks (5mg/kgintraperitoneally) from the beginning of the study. Specific humoral and cellular immunity, susceptibility of macrophages respiratory burst and proliferation of immune cells were measured. The Mann-Whitney test was used in order to compare using SPSS 19 software.
Findings The 17 beta-estradiol treated mice showed a significant increase in DTH reaction. MTT test results showed an increase in the rate of lymphocyte proliferation in estrogentreated group compared with control group. The spleen weight mean in treated mice group (28.62±1.76) showed a significant increase compared to control group (21.00±1.83) (p<0.01). The results of NBT reduction showed a decrease in spleen macrophages/monocyte respiratory burst capability.
Conclusion It seems that 17 beta-estradiol can be considered as the moderating immune system compound.
Materials & Methods 14 mice were divided into two groups and were immunized with SRBC antigen. The treated mice were injected with estrogen for two weeks (5mg/kgintraperitoneally) from the beginning of the study. Specific humoral and cellular immunity, susceptibility of macrophages respiratory burst and proliferation of immune cells were measured. The Mann-Whitney test was used in order to compare using SPSS 19 software.
Findings The 17 beta-estradiol treated mice showed a significant increase in DTH reaction. MTT test results showed an increase in the rate of lymphocyte proliferation in estrogentreated group compared with control group. The spleen weight mean in treated mice group (28.62±1.76) showed a significant increase compared to control group (21.00±1.83) (p<0.01). The results of NBT reduction showed a decrease in spleen macrophages/monocyte respiratory burst capability.
Conclusion It seems that 17 beta-estradiol can be considered as the moderating immune system compound.
CITATION LINKS
[1]Bini EI, Espinosa DM, Castillo BM, Payan JB, Colucci D, Cruz AF, et al. The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis. PLoS One. 2014;9(4):e93831.
[2]Eidinger D, Garrett TJ. Studies of the regulatory effects of the sex hormones on antibody formation and stem cell differentiation. J Exp Med. 1972;136(5):1098-116.
[3]Cutolo M, Seriolo B, Villaggio B, Pizzorni C, Craviotto C, Sulli A. Androgens and estrogens modulate the immune and inflammatory responses in rheumatoid arthritis. Ann N Y Acad Sci. 2002;966:131-42.
[4]Socas-Rodríguez B, Hernández-Borges J, Asensio-Ramos M, Herrera-Herrera AV, Palenzuela JA, Rodríguez-Delgado MA. Determination of estrogens in environmental water samples using 1,3-dipentylimidazolium hexafluorophosphate ionic liquid as extraction solvent in dispersive liquid-liquid microextraction. Electrophoresis. 2014;doi: 10.1002/elps.201400024
[5]Bukovsky A, Cekanova M, Caudle MR, Wimalasena J, Foster JS, Henley DC, et al. Expression and localization of estrogen receptor-alpha protein in normal and abnormal term placentae and stimulation of trophoblast differentiation by estradiol. Reprod Biol Endocrinol. 2003;1:13.
[6]Edelstam G, Karlsson C, Westgren M, Löwbeer C, Swahn ML. Human chorionic gonadatropin (hCG) during third trimester pregnancy. Scand J Clin Lab Invest. 2007;67(5):519-25.
[7]Nadkarni S, McArthur S. Oestrogen and immunomodulation: New mechanisms that impact on peripheral and central immunity. Curr Opin Pharmacol. 2013;13(4):576-81.
[8]Bouman A, Heineman MJ, Faas MM. Sex hormones and the immune response in humans. Hum Reprod Update. 2005;11(4):411-23.
[9]Shahabi N, Khaksari M. Effect of 17 beta-estradiol on wound healing in overiectomized rats. Koomesh. 2002;3(1-2):1-10. [Persian]
[10]Zimecki M, Wieczorek Z. Differential patterns of cyclosporine A-induced inhibition of humoral and cellular immune responses to sheep erythrocytes in mice. Pol J Pharmacol. 2001;53(5):495-500.
[11]Hassan ZM, Ebtekar M. Immunological consequence of sulfur mustard exposure. Immunol Lett. 2002;83(3):151-2. [Persian]
[12]Abtahi Froushani SM , Delirezh N, Hobbenaghi R , Mosayebi Gh. Therapeutic effects of all-trans retinoic acid on experimental autoimmune encephalomyelitis and its role in T-helper lymphocyte responses. Tehran Univ Med J. 2012;69(11):710-7.
[13]Abtahi Froushani SM, Delirezh N, Hobbenaghi R, Mosayebi G. Synergistic effects of atorvastatin and all-trans retinoic acid in ameliorating animal model of multiple sclerosis. Immunol Invest. 2014;43(1):54-68.
[14]Haq A, Lobo PI, Al-Tufail M, Rama NR, Al-Sedairy ST. Immunomodulatory effect of Nigella sativa proteins fractionated by ion exchange chromatography. Int J Immunopharmacol. 1999;21(4):283-95.
[15]Jones RE, Kaler L, Murphy S, Offner H. Tissue-dependent expression of estrogen receptor β in 17β-estradiol-mediated attenuation of autoimmune CNS inflammation. Open Autoimmun J. 2010;2:197-204.
[16]Offner H, Polanczyk M. A potential role for estrogen in experimental autoimmune encephalomyelitis and multiple sclerosis. Ann N Y Acad Sci. 2006;1089:343-72.
[17]Escribese MM, Kraus T, Rhee E, Fernandez-Sesma A, López CB, Moran TM. Estrogen inhibits dendritic cell maturation to RNA viruses. Blood. 2008;112(12):4574-84.
[18]Toyoda Y, Miyashita T, Endo S, Tsuneyama K, Fukami T, Nakajima M, Yokoi T. Estradiol and progesterone modulate halothane-induced liver injury in mice. Toxicol Lett. 2011;204(1):17-24.
[19]Murphy AJ, Guyre PM, Pioli PA. Estradiol suppresses NF-kappa B activation through coordinated regulation of let-7a and miR-125b in primary human macrophages. J Immunol. 2010;184(9):5029-37.
[20]Singh NP, Singh UP, Nagarkatti PS, Nagarkatti M. Prenatal exposure of mice to diethylstilbestrol disrupts T-cell differentiation by regulating Fas/Fas ligand expression through estrogen receptor element and nuclear factor-κB motifs. J Pharmacol Exp Ther. 2012;343(2):351-61.
[21]Ashcroft GS, Dodsworth J, van Boxtel E, Tarnuzzer RW, Horan MA, Schultz GS, et al. Estrogen accelerates cutaneous wound healing associated with an increase in TGF-beta1 levels. Nat Med. 1997;3(11):1209-15.
[22]Morales DE, McGowan KA, Grant DS, Maheshwari S, Bhartiya D, Cid MC, et al. Estrogen promotes angiogenic activity in human umbilical vein endothelial cells in vitro and in a murine model. Circulation. 1995;91(3):755-63.
[23]Pazos MA, Kraus TA, Muñoz-Fontela C, Moran TM. Estrogen mediates innate and adaptive immune alterations to influenza infection in pregnant mice. PLoS One. 2012;7(7):e40502.
[24]Zhang J, Chen X, Zhang S, Zhou G, Xia X, Lu L. Effects of transdermal estrogen therapy on expressions of estrogen receptors and T-lymphocyte apoptosis in surgically menopausal women. Cell Mol Immunol. 2009;6(4):277-83.
[25]Matalka KZ. The effect of estradiol, but not progesterone, on the production of cytokines in stimulated whole blood, is concentration-dependent. Neuro Endocrinol Lett. 2003;24(3-4):185-91.
[26]Xiong YH, Yuan Z, He L. Effects of estrogen on CD4(+) CD25(+) regulatory T cell in peripheral blood during pregnancy. Asian Pac J Trop Med. 2013;6(9):748-52.
[27]Fu Y, Li L, Liu X, Ma C, Zhang J, Jiao Y, You L, Chen ZJ, Zhao Y. Estrogen promotes B cell activation in vitro through down-regulating CD80 molecule expression. Gynecol Endocrinol. 2011;27(8)593-6.
[2]Eidinger D, Garrett TJ. Studies of the regulatory effects of the sex hormones on antibody formation and stem cell differentiation. J Exp Med. 1972;136(5):1098-116.
[3]Cutolo M, Seriolo B, Villaggio B, Pizzorni C, Craviotto C, Sulli A. Androgens and estrogens modulate the immune and inflammatory responses in rheumatoid arthritis. Ann N Y Acad Sci. 2002;966:131-42.
[4]Socas-Rodríguez B, Hernández-Borges J, Asensio-Ramos M, Herrera-Herrera AV, Palenzuela JA, Rodríguez-Delgado MA. Determination of estrogens in environmental water samples using 1,3-dipentylimidazolium hexafluorophosphate ionic liquid as extraction solvent in dispersive liquid-liquid microextraction. Electrophoresis. 2014;doi: 10.1002/elps.201400024
[5]Bukovsky A, Cekanova M, Caudle MR, Wimalasena J, Foster JS, Henley DC, et al. Expression and localization of estrogen receptor-alpha protein in normal and abnormal term placentae and stimulation of trophoblast differentiation by estradiol. Reprod Biol Endocrinol. 2003;1:13.
[6]Edelstam G, Karlsson C, Westgren M, Löwbeer C, Swahn ML. Human chorionic gonadatropin (hCG) during third trimester pregnancy. Scand J Clin Lab Invest. 2007;67(5):519-25.
[7]Nadkarni S, McArthur S. Oestrogen and immunomodulation: New mechanisms that impact on peripheral and central immunity. Curr Opin Pharmacol. 2013;13(4):576-81.
[8]Bouman A, Heineman MJ, Faas MM. Sex hormones and the immune response in humans. Hum Reprod Update. 2005;11(4):411-23.
[9]Shahabi N, Khaksari M. Effect of 17 beta-estradiol on wound healing in overiectomized rats. Koomesh. 2002;3(1-2):1-10. [Persian]
[10]Zimecki M, Wieczorek Z. Differential patterns of cyclosporine A-induced inhibition of humoral and cellular immune responses to sheep erythrocytes in mice. Pol J Pharmacol. 2001;53(5):495-500.
[11]Hassan ZM, Ebtekar M. Immunological consequence of sulfur mustard exposure. Immunol Lett. 2002;83(3):151-2. [Persian]
[12]Abtahi Froushani SM , Delirezh N, Hobbenaghi R , Mosayebi Gh. Therapeutic effects of all-trans retinoic acid on experimental autoimmune encephalomyelitis and its role in T-helper lymphocyte responses. Tehran Univ Med J. 2012;69(11):710-7.
[13]Abtahi Froushani SM, Delirezh N, Hobbenaghi R, Mosayebi G. Synergistic effects of atorvastatin and all-trans retinoic acid in ameliorating animal model of multiple sclerosis. Immunol Invest. 2014;43(1):54-68.
[14]Haq A, Lobo PI, Al-Tufail M, Rama NR, Al-Sedairy ST. Immunomodulatory effect of Nigella sativa proteins fractionated by ion exchange chromatography. Int J Immunopharmacol. 1999;21(4):283-95.
[15]Jones RE, Kaler L, Murphy S, Offner H. Tissue-dependent expression of estrogen receptor β in 17β-estradiol-mediated attenuation of autoimmune CNS inflammation. Open Autoimmun J. 2010;2:197-204.
[16]Offner H, Polanczyk M. A potential role for estrogen in experimental autoimmune encephalomyelitis and multiple sclerosis. Ann N Y Acad Sci. 2006;1089:343-72.
[17]Escribese MM, Kraus T, Rhee E, Fernandez-Sesma A, López CB, Moran TM. Estrogen inhibits dendritic cell maturation to RNA viruses. Blood. 2008;112(12):4574-84.
[18]Toyoda Y, Miyashita T, Endo S, Tsuneyama K, Fukami T, Nakajima M, Yokoi T. Estradiol and progesterone modulate halothane-induced liver injury in mice. Toxicol Lett. 2011;204(1):17-24.
[19]Murphy AJ, Guyre PM, Pioli PA. Estradiol suppresses NF-kappa B activation through coordinated regulation of let-7a and miR-125b in primary human macrophages. J Immunol. 2010;184(9):5029-37.
[20]Singh NP, Singh UP, Nagarkatti PS, Nagarkatti M. Prenatal exposure of mice to diethylstilbestrol disrupts T-cell differentiation by regulating Fas/Fas ligand expression through estrogen receptor element and nuclear factor-κB motifs. J Pharmacol Exp Ther. 2012;343(2):351-61.
[21]Ashcroft GS, Dodsworth J, van Boxtel E, Tarnuzzer RW, Horan MA, Schultz GS, et al. Estrogen accelerates cutaneous wound healing associated with an increase in TGF-beta1 levels. Nat Med. 1997;3(11):1209-15.
[22]Morales DE, McGowan KA, Grant DS, Maheshwari S, Bhartiya D, Cid MC, et al. Estrogen promotes angiogenic activity in human umbilical vein endothelial cells in vitro and in a murine model. Circulation. 1995;91(3):755-63.
[23]Pazos MA, Kraus TA, Muñoz-Fontela C, Moran TM. Estrogen mediates innate and adaptive immune alterations to influenza infection in pregnant mice. PLoS One. 2012;7(7):e40502.
[24]Zhang J, Chen X, Zhang S, Zhou G, Xia X, Lu L. Effects of transdermal estrogen therapy on expressions of estrogen receptors and T-lymphocyte apoptosis in surgically menopausal women. Cell Mol Immunol. 2009;6(4):277-83.
[25]Matalka KZ. The effect of estradiol, but not progesterone, on the production of cytokines in stimulated whole blood, is concentration-dependent. Neuro Endocrinol Lett. 2003;24(3-4):185-91.
[26]Xiong YH, Yuan Z, He L. Effects of estrogen on CD4(+) CD25(+) regulatory T cell in peripheral blood during pregnancy. Asian Pac J Trop Med. 2013;6(9):748-52.
[27]Fu Y, Li L, Liu X, Ma C, Zhang J, Jiao Y, You L, Chen ZJ, Zhao Y. Estrogen promotes B cell activation in vitro through down-regulating CD80 molecule expression. Gynecol Endocrinol. 2011;27(8)593-6.