@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2018;24(2):119-124
ISSN: 2252-0805 The Horizon of Medical Sciences 2018;24(2):119-124
Comparison the Effect of Placenta Membrane Using Alone or With Silver Sulfadiazine in Treatment of Burns in Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Samiee-Rad F (1)Beheshti A (2)
Zangivand AA (3)
Sofiabadi M (* 1)
(* 1) Cellular & Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
(1) Department of Pathology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
(2) Department of Dermatology, School of Medicine, Qazvin University of Medical Sciences, Qazvin , Iran
(3) School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Correspondence
Address: Physiology Department, Medicine Faculty, Qazvin University of Medical Sciences, Shahid Bahonar Boulevard, Qazvin, Iran.Phone: +98 (28) 33336001
Fax: +98 (28) 33324971
mohasofi@yahoo.com
Article History
Received: September 8, 2017Accepted: May 2, 2018
ePublished: May 16, 2018
ABSTRACT
Aims
Silver sulfadiazine is widely used for burning wound. Studies have shown that, dressing wounds with placental membrane can accelerate their healing. Our objective was to evaluate the recovery of second-degree burns in male rats that treated with topical Silver sulfadiazine-impregnated placental amniotic membrane.
Materials & Methods In this experimental study, 64 male rats were divided into 4 similar groups. In all rats, the second degree burn was induced under general anesthesia. Wound dressing in the first group (control) included simple dressing, but amniotic membrane in second group, and Silver sulfadiazine in third group and in the fourth group it was Silver sulfadiazine impregnated amniotic membrane. At the end of the 7th, 14th, 21st and 28th day, under the general anesthesia, the tissue biopsies were taken from burns and microscopic evaluation was done. Data were analyzed statistically using ANOVA and P less than 0.05 was considered as significant.
Findings In treated groups, the PMN leukocyte count was significantly lower than control (p<0.001), also the amount of granular formation and its organization, the number of fibroblasts, and the rate of wound healing were greater than control (p<0.001). There was no significant difference in measured parameters between the 3 treatment groups.
Conclusion Considering the biological effects of the ammonium membrane, it is recommended to use it as an effective dressing method to accelerate burn wound healing.
Materials & Methods In this experimental study, 64 male rats were divided into 4 similar groups. In all rats, the second degree burn was induced under general anesthesia. Wound dressing in the first group (control) included simple dressing, but amniotic membrane in second group, and Silver sulfadiazine in third group and in the fourth group it was Silver sulfadiazine impregnated amniotic membrane. At the end of the 7th, 14th, 21st and 28th day, under the general anesthesia, the tissue biopsies were taken from burns and microscopic evaluation was done. Data were analyzed statistically using ANOVA and P less than 0.05 was considered as significant.
Findings In treated groups, the PMN leukocyte count was significantly lower than control (p<0.001), also the amount of granular formation and its organization, the number of fibroblasts, and the rate of wound healing were greater than control (p<0.001). There was no significant difference in measured parameters between the 3 treatment groups.
Conclusion Considering the biological effects of the ammonium membrane, it is recommended to use it as an effective dressing method to accelerate burn wound healing.
CITATION LINKS
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[2]Atiyeh BS, Amm CA, El Musa KA. Improved scar quality following primary and secondary healing of cutaneous wounds. Aesthetic Plast Surg. 2003;27(5):411-7.
[3]Finnson KW, Mc Lean S, Di Guglielmo GM, Philip A. Dynamics of transforming growth factor beta signaling in wound healing and scarring. Adv Wound Care (New Rochelle). 2013;2(5):195-214.
[4]Atiyeh BS, Gunn SW, Hayek SN. State of the art in burn treatment. World J Surg. 2005;29(2):131-48.
[5]Das S, Baker AB. Biomaterials and nanotherapeutics for enhancing skin wound healing. Front Bioeng Biotechnol. 2016;4:82.
[6]Ramirez H, Patel SB, Pastar I. The role of TGFβ signaling in wound epithelialization. Adv Wound Care (New Rochelle). 2014;3(7):482-91.
[7]Tyszkiewicz JT, Uhrynowska-Tyszkiewicz IA, Kaminski A, Dziedzic-Goclawska A. Amnion allografts prepared in the Central Tissue Bank in Warsaw. Ann Transplant. 1999;4(3-4):85-90.
[8]Rinastiti M, Harijadi, Santoso AL, Sosroseno W. Histological evaluation of rabbit gingival wound healing transplanted with human amniotic membrane. Int J Oral Maxillofac Surg. 2006;35(3):247-51.
[9]Venkataraman M, Nagarsenker M. Silver sulfadiazine nanosystems for burn therapy. AAPS PharmSciTech. 2013;14(1):254-64.
[10]De Gracia CG. An open study comparing topical silver sulfadiazine and topical silver sulfadiazine-cerium nitrate in the treatment of moderate and severe burns. Burns. 2001;27(1):67-74.
[11]Lee ARC, Moon HK. Effect of topically applied silver sulfadiazine on fibroblast cell proliferation and biomechanical properties of the wound. Arch Pharm Res. 2003;26(10):855-60.
[12]Tumino G, Masuelli L, Bei R, Simonelli L, Santoro A, Francipane S. Topical treatment of chronic venous ulcers with sucralfate: A placebo controlled randomized study. Int J Mol Med. 2008;22(1):17-23.
[13]Shahabi Sh, Zahir MH, Hashemi SM, Shahrokhi S, Karimipour M, Kazem Nejad A, et al. Hyperthermia can accelerate the healing process of 2nd degree burn wounds. J Kerman Univ Med Sci. 2005;12(2):110-8. [Persian]
[14]Toussaint J, Chung WT, Osman N, Mc Clain SA, Raut V, Singer AJ. Topical antibiotic ointment versus silver-containing foam dressing for second-degree burns in swine. Acad Emerg Med. 2015;22(8):927-33.
[15]Talas DU, Nayci A, Atis S, Polat A, Comelekoglu U, Bagdatoglu C, et al. The effects of corticosteroids on the healing of tracheal anastomoses in a rat model. Pharmacol Res. 2002;45(4):299-304.
[16]Salehi SH, Asadi K, Mousavi SJ. Evolution of effective of amniotic membrane dressing vs. conventional topical antibiotic dressing of skin graft donor site in burn patients. Iran J Surg. 2011;19(1):16-23. [Persian]
[17]Mohammadi AA, Riazi H, Hasheminasab MJ, Sabet B, Mohammadi MK, Abbasi S, et al. Amniotic membrane dressing vs conventional topical antibiotic dressing in hospitalized burn patients. Iran Red Crescent Med J. 2009;11(1):66-70.
[18]Mostaque AK, Rahman KB. Comparisons of the effects of biological membrane (amnion) and silver sulfadiazine in the management of burn wounds in children. J Burn Care Res. 2011;32(2):200-9.
[19]Al-Waili N, Salom K, Al-Ghamdi AA. Honey for wound healing, ulcers, and burns, data supporting its use in clinical practice. Sci World J. 2011;11:766-87.
[20]Halim AS, Khoo TL, Yussof SJM. Biologic and synthetic skin substitutes: An overview. Indian J Plast Surg. 2010;43(Suppl):S23-8.
[21]Park WC, Tseng SC. Modulation of acute inflammation and keratocyte death by suturing, blood, and amniotic membrane in PRK. Invest Ophthalmol Vis Sci. 2000;41(10):2906-14.
[22]Al-Waili NS. Effects of honey on the urinary total nitrite and prostaglandins concentration. Int Urol Nephrol. 2005;37(1):107-11.
[23]Kobayashi N, Kabuyama Y, Sasaki S, Kato K, Homma Y. Suppression of corneal neovascularization by culture supernatant of human amniotic cells. Cornea. 2002;21(1):62-7.
[24] Yam HF, Pang CP, Fan DS, Fan BJ, Yu EY, Lam DS. Growth factor changes in ex vivo expansion of human limbal epithelial cells on human amniotic membrane. Cornea. 2002;21(1):101-5.
[25]Cho Lee AR, Leem H, Lee J, Park KC. Reversal of silver sulfadiazine-impaired wound healing by epidermal growth factor. Biomaterials. 2005;26(22):4670-6.
[26]Du Toit DF, Page BJ. An in vitro evaluation of the cell toxicity of honey and silver dressings. J Wound Care. 2009;18(9):383-9.
[27]Kumar PM, Ghosh A. Development and evaluation of silver sulfadiazine loaded microsponge based gel for partial thickness (second degree) burn wounds. Eur J Pharm Sci. 2017;96:243-54.
[2]Atiyeh BS, Amm CA, El Musa KA. Improved scar quality following primary and secondary healing of cutaneous wounds. Aesthetic Plast Surg. 2003;27(5):411-7.
[3]Finnson KW, Mc Lean S, Di Guglielmo GM, Philip A. Dynamics of transforming growth factor beta signaling in wound healing and scarring. Adv Wound Care (New Rochelle). 2013;2(5):195-214.
[4]Atiyeh BS, Gunn SW, Hayek SN. State of the art in burn treatment. World J Surg. 2005;29(2):131-48.
[5]Das S, Baker AB. Biomaterials and nanotherapeutics for enhancing skin wound healing. Front Bioeng Biotechnol. 2016;4:82.
[6]Ramirez H, Patel SB, Pastar I. The role of TGFβ signaling in wound epithelialization. Adv Wound Care (New Rochelle). 2014;3(7):482-91.
[7]Tyszkiewicz JT, Uhrynowska-Tyszkiewicz IA, Kaminski A, Dziedzic-Goclawska A. Amnion allografts prepared in the Central Tissue Bank in Warsaw. Ann Transplant. 1999;4(3-4):85-90.
[8]Rinastiti M, Harijadi, Santoso AL, Sosroseno W. Histological evaluation of rabbit gingival wound healing transplanted with human amniotic membrane. Int J Oral Maxillofac Surg. 2006;35(3):247-51.
[9]Venkataraman M, Nagarsenker M. Silver sulfadiazine nanosystems for burn therapy. AAPS PharmSciTech. 2013;14(1):254-64.
[10]De Gracia CG. An open study comparing topical silver sulfadiazine and topical silver sulfadiazine-cerium nitrate in the treatment of moderate and severe burns. Burns. 2001;27(1):67-74.
[11]Lee ARC, Moon HK. Effect of topically applied silver sulfadiazine on fibroblast cell proliferation and biomechanical properties of the wound. Arch Pharm Res. 2003;26(10):855-60.
[12]Tumino G, Masuelli L, Bei R, Simonelli L, Santoro A, Francipane S. Topical treatment of chronic venous ulcers with sucralfate: A placebo controlled randomized study. Int J Mol Med. 2008;22(1):17-23.
[13]Shahabi Sh, Zahir MH, Hashemi SM, Shahrokhi S, Karimipour M, Kazem Nejad A, et al. Hyperthermia can accelerate the healing process of 2nd degree burn wounds. J Kerman Univ Med Sci. 2005;12(2):110-8. [Persian]
[14]Toussaint J, Chung WT, Osman N, Mc Clain SA, Raut V, Singer AJ. Topical antibiotic ointment versus silver-containing foam dressing for second-degree burns in swine. Acad Emerg Med. 2015;22(8):927-33.
[15]Talas DU, Nayci A, Atis S, Polat A, Comelekoglu U, Bagdatoglu C, et al. The effects of corticosteroids on the healing of tracheal anastomoses in a rat model. Pharmacol Res. 2002;45(4):299-304.
[16]Salehi SH, Asadi K, Mousavi SJ. Evolution of effective of amniotic membrane dressing vs. conventional topical antibiotic dressing of skin graft donor site in burn patients. Iran J Surg. 2011;19(1):16-23. [Persian]
[17]Mohammadi AA, Riazi H, Hasheminasab MJ, Sabet B, Mohammadi MK, Abbasi S, et al. Amniotic membrane dressing vs conventional topical antibiotic dressing in hospitalized burn patients. Iran Red Crescent Med J. 2009;11(1):66-70.
[18]Mostaque AK, Rahman KB. Comparisons of the effects of biological membrane (amnion) and silver sulfadiazine in the management of burn wounds in children. J Burn Care Res. 2011;32(2):200-9.
[19]Al-Waili N, Salom K, Al-Ghamdi AA. Honey for wound healing, ulcers, and burns, data supporting its use in clinical practice. Sci World J. 2011;11:766-87.
[20]Halim AS, Khoo TL, Yussof SJM. Biologic and synthetic skin substitutes: An overview. Indian J Plast Surg. 2010;43(Suppl):S23-8.
[21]Park WC, Tseng SC. Modulation of acute inflammation and keratocyte death by suturing, blood, and amniotic membrane in PRK. Invest Ophthalmol Vis Sci. 2000;41(10):2906-14.
[22]Al-Waili NS. Effects of honey on the urinary total nitrite and prostaglandins concentration. Int Urol Nephrol. 2005;37(1):107-11.
[23]Kobayashi N, Kabuyama Y, Sasaki S, Kato K, Homma Y. Suppression of corneal neovascularization by culture supernatant of human amniotic cells. Cornea. 2002;21(1):62-7.
[24] Yam HF, Pang CP, Fan DS, Fan BJ, Yu EY, Lam DS. Growth factor changes in ex vivo expansion of human limbal epithelial cells on human amniotic membrane. Cornea. 2002;21(1):101-5.
[25]Cho Lee AR, Leem H, Lee J, Park KC. Reversal of silver sulfadiazine-impaired wound healing by epidermal growth factor. Biomaterials. 2005;26(22):4670-6.
[26]Du Toit DF, Page BJ. An in vitro evaluation of the cell toxicity of honey and silver dressings. J Wound Care. 2009;18(9):383-9.
[27]Kumar PM, Ghosh A. Development and evaluation of silver sulfadiazine loaded microsponge based gel for partial thickness (second degree) burn wounds. Eur J Pharm Sci. 2017;96:243-54.