@2024 Afarand., IRAN
ISSN: 2251-8215 Sarem Journal of Reproductive Medicine 2018;2(4):165-168
ISSN: 2251-8215 Sarem Journal of Reproductive Medicine 2018;2(4):165-168
Presence of Group B Streptococcus in the Placenta of Infected Pregnant Women
ARTICLE INFO
Article Type
Original ResearchAuthors
Rahimi M. (1)Saadatnia G. (*)
Salehian P. (2)
(*) Biotechnology Research Institute, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
(1) Basic Science Faculty, Science and Research Branch, Islamic Azad University, Tehran, Iran
(2) Dr. Salehian medical laboratory, Tehran, Iran
Correspondence
Article History
Received: April 29, 2017Accepted: October 11, 2017
ePublished: November 15, 2018
ABSTRACT
Aims
Group B Streptococcus (GBS) is one of the most important causes of neonatal infections. This bacterial pathogen is associated with preterm birth and neonatal mortality. This study was aimed to evaluate the presence of GBS in the placenta of infected pregnant women.
Materials & Methods This analytical case-control study was conducted on pregnant patients referred to Sarem women’s hospital (Tehran, Iran) for delivery during 2013-2014. A total of 80 formalin-fixed and paraffin-embedded placenta tissues were studied, including 55 samples from GBS positive patients (30 samples with chorioamnionitis and 25 without chorioamnionitis) and 25 samples from GBS negative subjects with chorioamnionitis as the controls. The presence of GBS for each sample was tested by polymerase chain reaction (PCR) and 1.5% agarose gel electrophoresis.
Findings GBS genomic DNA was not detected in any sample from the placenta tissue.
Conclusion Group B Streptococcus is not present in the placenta of the women infected with GBS (with or without amniotic membrane inflammation).
Materials & Methods This analytical case-control study was conducted on pregnant patients referred to Sarem women’s hospital (Tehran, Iran) for delivery during 2013-2014. A total of 80 formalin-fixed and paraffin-embedded placenta tissues were studied, including 55 samples from GBS positive patients (30 samples with chorioamnionitis and 25 without chorioamnionitis) and 25 samples from GBS negative subjects with chorioamnionitis as the controls. The presence of GBS for each sample was tested by polymerase chain reaction (PCR) and 1.5% agarose gel electrophoresis.
Findings GBS genomic DNA was not detected in any sample from the placenta tissue.
Conclusion Group B Streptococcus is not present in the placenta of the women infected with GBS (with or without amniotic membrane inflammation).
CITATION LINKS
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[12]Naeye RL, Peters EC. Causes and consequences of premature rupture of fetal membranes. Lancet. 1980;1(8161):192-4.
[13]McDonald HM, Chambers HM. Intrauterine infection and spontaneous midgestation abortion: Is the spectrum of microorganisms similar to that in preterm labor?. Infect Diseas Obstet Gynecol. 2000;8(5-6):220-7.
[14]Zlatnik FJ, Gellhaus TM, Benda JA, Koontz FP, Burmeister LF. Histologic chorioamnionitis, microbial infection, and prematurity. Obstet Gynecol. 1990;76(3-1):355-9.
[15]Dunlow SG, Duff P. Microbiology of the lower genital tract and amniotic fluid in asymptomatic preterm patients with intact membranes and moderate to advanced degrees of cervical effacement and dilation. Am J Perinatol. 1990;7(3):235-8.
[16]Wahbeh CJ, Hill GB, Eden RD, Gall SA. Intra-amniotic bacterial colonization in premature labor. Am J Obstet Gynecol. 1984;148(6):739-43.
[2]Allen U, Nimrod C, Macdonald N, Toye B, Stephens D, Marchessault V. Relationship between antenatal group B streptococcal vaginal colonization and premature labour. Paediatr Child Health. 1999;4(7):465-9.
[3]Schuchat A. Epidemiology of group B streptococcal disease in the United States: Shifting Paradigms. Clin Microbiol Rev. 1998;11(3):497-513.
[4]Convert M, Martinetti Lucchini G, Dolina M, Piffaretti JC. Comparison of Light Cycler PCR and culture for detection of group B streptococci from vaginal swabs. Clin Microbiol Infec. 2005;11(12):1022-6.
[5]Shet A, Ferrieri P. Neonatal and maternal group B streptococcal infections: A comprehensive review. Indian J Med Res. 2004;120(3):141-50.
[6]Rallu F, Barriga P, Scrivo C, Martel Laferrière V, Laferrière C. Sensitivities of antigen detection and PCR assays greatly increased compared to that of the standard culture method for screening for group B streptococcus carriage in pregnant women. J Clin Microbiol. 2006;44(3):725-8.
[7]López Sastre JB, Fernández Colomer B, Coto Cotallo GD, Ramos Aparicio A. Trends in the epidemiology of neonatal sepsis of vertical transmission in the era of group B streptococcal prevention. Acta Paediatr. 2005;94(4):451-7.
[8]Schuchat A, Whitney C, Zangwill K. Prevention of perinatal group B streptococcal disease: A public health perspective. Morbid Mortal Week Report. 1996;45(7):1-24.
[9]Nazer M, Rafiei Alavi E, Nazer E, Khamechi M. Prevalence of Group B Streptococcus Vaginal Colonization in The Third Trimester of Pregnancy. J Shahid Sadoughi Univ Med Sci. 2011;19(1):13-23. [Persian]
[10]Valkenburg van den Berg AW, Sprij AJ, Dekker FW, Dorr PJ, Kanhai HH. Association between colonization with Group B Streptococcus and preterm delivery: A systematic review. Acta Obstet Gynecol Scand. 2009;88(9):958-67.
[11]Foruhesh Tehrani H, Saadat S, Rikhtehgaran Z. Diagnostic microbiology: Principles of infection in different body organs. Tehran: Ibn Sina; 2012. [Persian]
[12]Naeye RL, Peters EC. Causes and consequences of premature rupture of fetal membranes. Lancet. 1980;1(8161):192-4.
[13]McDonald HM, Chambers HM. Intrauterine infection and spontaneous midgestation abortion: Is the spectrum of microorganisms similar to that in preterm labor?. Infect Diseas Obstet Gynecol. 2000;8(5-6):220-7.
[14]Zlatnik FJ, Gellhaus TM, Benda JA, Koontz FP, Burmeister LF. Histologic chorioamnionitis, microbial infection, and prematurity. Obstet Gynecol. 1990;76(3-1):355-9.
[15]Dunlow SG, Duff P. Microbiology of the lower genital tract and amniotic fluid in asymptomatic preterm patients with intact membranes and moderate to advanced degrees of cervical effacement and dilation. Am J Perinatol. 1990;7(3):235-8.
[16]Wahbeh CJ, Hill GB, Eden RD, Gall SA. Intra-amniotic bacterial colonization in premature labor. Am J Obstet Gynecol. 1984;148(6):739-43.