@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(1):21-29
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(1):21-29
Effect of Aqueous Extract of Launaea acanthodes on Testicular Tissue and Sperm Parameters in Alloxan-Induced Diabetic Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Rahbarian R. (1 )Sepehri Moghadam H. (2 )
Sadoughi S.D. (* )
(* ) Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran
(1 ) Biology Department, Sciences Faculty, Payam-e-Noor University, Tehran, Iran
(2 ) Agriculture Department, Sciences Faculty, Payam-e-Noor University, Tehran, Iran
Correspondence
Address: Biology Department, Sciences Faculty, Payam-e-Noor University, Mo’allem Boulevard, Mashhad, Iran. Post Box: 91735-433Phone: +985135013950
Fax: +985135013950
damoon.Sadughi@gmail.com
Article History
Received: November 22, 2014Accepted: February 28, 2015
ePublished: April 16, 2015
ABSTRACT
Aims
Diabetes cause oxidative stress in sperm and testicular tissue. The
Launaea acanthodes has anti-oxidant and anti-diabetic effects. The present
study was done to evaluate the effect of Launaea acanthodes extract on sperm
parameters and testicular tissue in diabetic rats.
Materials & Methods 27 rats were divided into the 3 equal groups; control, diabetic control and experimental diabetic. Experimental diabetic and diabetic control groups were got diabetic by an intraperitoneal injection of alloxan. Extract of Launaea acanthodes with concentration of 300mg/kg was injected intraperitoneally to the experimental diabetic group every other day for a month. Sterile distilled water was injected to control and diabetic control groups. After creating experimental diabetes, all injections were done every other day for a month. On day 30, all rats were sacrificed and their testes were removed for assessment of sperm parameters and histological evaluation. Data were analyzed by SPSS 20 software using Kruskal Wallis and Dunn post hoc test.
Findings Percentage of progressive motility, natural forms and the number of sperms in treated diabetic group with extract of Launaea acanthodes with concentration of 300mg/kg were increased significantly compared to the diabetic control group (p<0.05). Average weight, length, width and average diameter of the tubules of the testes and the average thickness of the epithelium of the seminiferous tubule in diabetic group treated with extract of Launaea acanthodes were increased significantly compared to the diabetic control group (p<0.05).
Conclusion Extract of Launaea acanthodes improves sperm parameters, increase sperm count and decrease atrophy of seminiferous tubules of diabetic rats.
Materials & Methods 27 rats were divided into the 3 equal groups; control, diabetic control and experimental diabetic. Experimental diabetic and diabetic control groups were got diabetic by an intraperitoneal injection of alloxan. Extract of Launaea acanthodes with concentration of 300mg/kg was injected intraperitoneally to the experimental diabetic group every other day for a month. Sterile distilled water was injected to control and diabetic control groups. After creating experimental diabetes, all injections were done every other day for a month. On day 30, all rats were sacrificed and their testes were removed for assessment of sperm parameters and histological evaluation. Data were analyzed by SPSS 20 software using Kruskal Wallis and Dunn post hoc test.
Findings Percentage of progressive motility, natural forms and the number of sperms in treated diabetic group with extract of Launaea acanthodes with concentration of 300mg/kg were increased significantly compared to the diabetic control group (p<0.05). Average weight, length, width and average diameter of the tubules of the testes and the average thickness of the epithelium of the seminiferous tubule in diabetic group treated with extract of Launaea acanthodes were increased significantly compared to the diabetic control group (p<0.05).
Conclusion Extract of Launaea acanthodes improves sperm parameters, increase sperm count and decrease atrophy of seminiferous tubules of diabetic rats.
CITATION LINKS
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[13]Hajinejad Boshroue R, Behnam Rassouli M, Tehranipour M, Gheybi F, Hajinejad Sh, Elahi Moghadam Z. The effects of hydro-alcoholic extract of launaea acanthodes on the blood, urine albumin and bilirubin levels in male hyperglycemic wistar rat. Iranian J Endocrinol Metabolism. 2013;15(2):190-6. [Persian]
[14]Piazza L, Bertini S, Milany J. Extraction and structural characterization of the polysaccharide fraction of Launaea acanthodes gum. Carbohydr Polymers. 2010;79(2):449-54.
[15]Karimidokht Shahrbabaki A, Oryan Sh, Parivar K. Anticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice. J Qazvin Univ Med Sci. 2009;13(1):14- 20. [Persian]
[16]Sadooghi SD, Nezhad Shahrokh Abadi Kh, Zafar Balanzhad S, Baharara J. Investigating the cytotoxic effect of ethanolic extract of Ferula assa-foetida resin on HepG2 cell line. Feyz. 2013;17(4):323-30. [Persian]
[17]Kheradmand A. Improvement of sperm evaluation parameters following ghrelin treatment in cadmiuminduced testicular injury in rats. J Isfahan Med Sch. 2014;31(265):2053-62. [Persian]
[18]Tajaddini Sh, Ebrahimi S, Shirinbayan P, Bakhtiyari M, Behnam B, Joghataei MT, et al. Protective effects of manganese on the testis structure and sperm parameters of formalin-treated mice. J Isfahan Med Sch. 2013;31(243):1018-32. [Persian]
[19]Behnam-Rassouli M, Ghayour N, Ghayour MM, Ejtehadi MM. Investigating the effects of hydro-alcoholic extract of Launaea acanthodes on the serum levels of glucose, insulin, lipids and lipoproteins in stereptozotocin induced type I diabetic rats. Arak Med Univ J. 2012;14(6):48-56. [Persian]
[20]Yousef MI. Protective role of ascorbic acid to enhance reproductive performance of male rabbits treated with stannous chloride. Toxicol. 2005;207(1):81-9.
[21]Shiva M, Gautam AK, Verma Y, Shivgotra V, Doshi H, Kumar S. Association between sperm quality, oxidative stress, and seminal antioxidant activity. Clin Biochemistry. 2011;44(4):319-24.
[22]Zhong RZh, Zhou DW. Oxidative stress and role of natural plant derived antioxidants in animal reproduction. J Integr Agric. 2013;12(10):1826-38.
[23]Stefanović A, Kotur-Stevuljević J, Spasić S, Bogavac- Stanojević N, Bujisić N. The influence of obesity on the oxidative stress status and the concentration of leptin in type 2 diabetes mellitus patients. Diabetes Res Clin Pract. 2008;79(1):156-63.
[2]Rochette L, Zeller M, Cottin Y, Vergely C. Diabetes, oxidative stress and therapeutic strategies. Biochimica Biophysica Acta. 2014;1840(9):2709-29.
[3]Fanaei H, Keshtgar S, Bahmanpour S, Ghannadi A, Kazeroni M. Beneficial effects of α-tocopherol against intracellular calcium overload in human sperm. Reprod Sci. 2011;18(10):978-82.
[4]Lombardo F, Sansone A, Romanelli F, Paoli D, Gandini L, Lenzi A. The role of antioxidant therapy in the treatment of male infertility: An overview. Asian J Androl. 2011;13(5):690-7.
[5]Yang H, Jin X, Kei Lam CW, Yan SK. Oxidative stress and diabetes mellitus. Clin Chem Lab Med. 2011;49(11):1773-82.
[6]Cui X. Reactive oxygen species: The achilles’ heel of cancer cells? Antioxid Redox Signal. 2012;16(11):1212-4.
[7]Saleh RA, Agarwal A. Oxidative stress and male infertility: From research bench to clinical practice. J Androl. 2002;23(6):737-52.
[8]Tvrdá E, Kňažická Z, Bárdos L, Massányi P, Lukáč N. Impact of oxidative stress on male fertility - a review. Acta Vet Hung. 2011;59(4):465-84.
[9]Ford WC. Regulation of sperm function by reactive oxygen species. Hum Reprod Update. 2004;10(5):387- 99.
[10]Roessner C, Paasch U, Kratzsch J, Glander H, Grunewald S. Sperm apoptosis signalling in diabetic men. Reprod Biomed Online. 2012;25(3):292-9.
[11]Kilarkaje N, Al-Hussaini H, Al-Bader MM. Diabetesinduced DNA damage and apoptosis are associated with poly (ADP ribose) polymerase 1 inhibition in the rat testis. Eur J Pharmacol. 2014;737:29-40.
[12]Yun JI, Gong SP, Song YH, Lee ST. Effects of combined antioxidant supplementation on human sperm motility and morphology during sperm manipulation in vitro. Fertil Sterility. 2013;100(2):373-8.
[13]Hajinejad Boshroue R, Behnam Rassouli M, Tehranipour M, Gheybi F, Hajinejad Sh, Elahi Moghadam Z. The effects of hydro-alcoholic extract of launaea acanthodes on the blood, urine albumin and bilirubin levels in male hyperglycemic wistar rat. Iranian J Endocrinol Metabolism. 2013;15(2):190-6. [Persian]
[14]Piazza L, Bertini S, Milany J. Extraction and structural characterization of the polysaccharide fraction of Launaea acanthodes gum. Carbohydr Polymers. 2010;79(2):449-54.
[15]Karimidokht Shahrbabaki A, Oryan Sh, Parivar K. Anticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice. J Qazvin Univ Med Sci. 2009;13(1):14- 20. [Persian]
[16]Sadooghi SD, Nezhad Shahrokh Abadi Kh, Zafar Balanzhad S, Baharara J. Investigating the cytotoxic effect of ethanolic extract of Ferula assa-foetida resin on HepG2 cell line. Feyz. 2013;17(4):323-30. [Persian]
[17]Kheradmand A. Improvement of sperm evaluation parameters following ghrelin treatment in cadmiuminduced testicular injury in rats. J Isfahan Med Sch. 2014;31(265):2053-62. [Persian]
[18]Tajaddini Sh, Ebrahimi S, Shirinbayan P, Bakhtiyari M, Behnam B, Joghataei MT, et al. Protective effects of manganese on the testis structure and sperm parameters of formalin-treated mice. J Isfahan Med Sch. 2013;31(243):1018-32. [Persian]
[19]Behnam-Rassouli M, Ghayour N, Ghayour MM, Ejtehadi MM. Investigating the effects of hydro-alcoholic extract of Launaea acanthodes on the serum levels of glucose, insulin, lipids and lipoproteins in stereptozotocin induced type I diabetic rats. Arak Med Univ J. 2012;14(6):48-56. [Persian]
[20]Yousef MI. Protective role of ascorbic acid to enhance reproductive performance of male rabbits treated with stannous chloride. Toxicol. 2005;207(1):81-9.
[21]Shiva M, Gautam AK, Verma Y, Shivgotra V, Doshi H, Kumar S. Association between sperm quality, oxidative stress, and seminal antioxidant activity. Clin Biochemistry. 2011;44(4):319-24.
[22]Zhong RZh, Zhou DW. Oxidative stress and role of natural plant derived antioxidants in animal reproduction. J Integr Agric. 2013;12(10):1826-38.
[23]Stefanović A, Kotur-Stevuljević J, Spasić S, Bogavac- Stanojević N, Bujisić N. The influence of obesity on the oxidative stress status and the concentration of leptin in type 2 diabetes mellitus patients. Diabetes Res Clin Pract. 2008;79(1):156-63.