@2024 Afarand., IRAN
ISSN: 2251-8215 Sarem Journal of Reproductive Medicine 2017;1(3):113-116
ISSN: 2251-8215 Sarem Journal of Reproductive Medicine 2017;1(3):113-116
Human Papiloma Virus Detection in Various Cervical Lesions by Molecular Methods
ARTICLE INFO
Article Type
Original ResearchAuthors
Hasani M. (*)Salehian P. (1)
Pourazar Sh. (2)
(*) Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
(1) Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
(2) Masoud's Pathobiology Lab, Tehran, Iran
Correspondence
Article History
Received: March 15, 2016Accepted: June 14, 2016
ePublished: August 15, 2017
ABSTRACT
Aims
Human Papilloma Virus (HPV) is known as one of the causes of anogenital cancers, including cervix cancer. The inability of viral culture has made the initial detection of HPV difficult. Therefore, molecular methods are the best way to identify, and among these methods, Polymerase Chain Reaction (PCR) amplification methods are very useful and accurate in diagnosis. The aim of this study was to investigate the presence of HPV in Iranian patients and the relationship between the type of virus (high risk and low risk) and cervical lesions by molecular methods based on PCR virus samples.
Materials & Methods The present study was conducted on 67 biopsy and Liquid base cytological samples including cervical Liquid cytology and cervical tissue biopsy. The cytological diagnostic test and pathological examination were performed and the DNA was extracted and then, DNA typing was conducted by PCR using primers MY09/MY11, specific for capsid gene.
Findings Out of 67 patients, 2 cases (3.0%) were not infected with HPV, while cervical cytology was symptomatic. Seven cases had no symptoms in the cervical epithelial cells, which were virtually positive for the virus, and the rest also had both viral and clinical symptoms. Most of the samples (52.2%) were infected with high-risk viruses and, most of them were HPV16.
Conclusion HPV can be the main responsible for the cervical cells changes, that has the ability to become cancerous. Also, in most cases, the main cause of the infection is high-risk type of the virus. The most prevalent type of HPV that infected cervical epithelial cells is HPV16.
Materials & Methods The present study was conducted on 67 biopsy and Liquid base cytological samples including cervical Liquid cytology and cervical tissue biopsy. The cytological diagnostic test and pathological examination were performed and the DNA was extracted and then, DNA typing was conducted by PCR using primers MY09/MY11, specific for capsid gene.
Findings Out of 67 patients, 2 cases (3.0%) were not infected with HPV, while cervical cytology was symptomatic. Seven cases had no symptoms in the cervical epithelial cells, which were virtually positive for the virus, and the rest also had both viral and clinical symptoms. Most of the samples (52.2%) were infected with high-risk viruses and, most of them were HPV16.
Conclusion HPV can be the main responsible for the cervical cells changes, that has the ability to become cancerous. Also, in most cases, the main cause of the infection is high-risk type of the virus. The most prevalent type of HPV that infected cervical epithelial cells is HPV16.
CITATION LINKS
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[7]Park JS, Namkoong SE, Lee JM, Kim EJ, Chee YH, Han GT, et al. Cervical intraepithelial neoplasia 3, coinfected with HPV-16 and -18 -case report. J Korean Med Sci. 1993;8(2):162–5.
[8]Garland SM, Tabrizi S. Methods for HPV detection: Polymerase chain reaction assays. In: Monsonego J, editor. Emerging issues on HPV infections: From science to practice. Switzerland: Karger; 2006. pp. 63-72.
[9]Sterling JC, Kurtz JB. Viral infections. In: Champion RH, Burton JL, Burns DA, Breathnach SM. Textbook of Dermatology. 8th Edition. London: Blackwell Science; 1998. pp. 995-1095.
[10]Solomon D, Schiffman M, Tarone R, ALTS Study group. Comparison of three management strategies for patients with atypical squamous cells of undetermined significance: Baseline results from a randomized trial. J Natl Cancer Inst. 2001;93(4):293-9.
[11]Cope JU, Hildesheim A, Schiffman MH, Manos MM, Lörincz AT, Burk RD, et al. Comparison of the hybrid capture tube test and PCR for detection of human papillomavirus DNA in cervical specimens. J Clin Microbiol. 1997;35(9):2262-5.
[12]Nomelini RS, Barcelos AC, Michelin MA, Adad SJ, Murta EF. Utilization of human papillomavirus testing for cervical cancer prevention in a university hospital. Cad Saude Publica. 2007;23(6):1309-18.
[2]Malloy C, Sherris J, Herdman C. HPV DNA testing: Technical and programmatic issues for cervical cancer prevention in low-resource settings [Internet]. Semantic Scholar: 2015 [updated 2000 Dec 1; cited 2001 Dec 2]. Avilable from: https://goo.gl/7VKtgJ.
[3]Definitions & Characteristics of HPV: Merck Medicos Modules; 2006.
[4]James WD, Berger T, Dirk M. Andrews’ Diseases of the Skin. 9th edition. Philadelphia: Saunders; 2000.
[5]Czegledy J, Gergely L, Hernadi Z, Poka R. Detection of human papillomavirus deoxyribonucleic acid in the female genital tract. Med Microbiol Immunol. 1989;178(6):309-14.
[6]Nasiri S, Ghalamkarpoor F, Saberi A, Parvaneh V. Study of human papilloma virus in anogenital condylomas by PCR method. Iran J Clin Infect Dis. 2008;3(1):19-23.
[7]Park JS, Namkoong SE, Lee JM, Kim EJ, Chee YH, Han GT, et al. Cervical intraepithelial neoplasia 3, coinfected with HPV-16 and -18 -case report. J Korean Med Sci. 1993;8(2):162–5.
[8]Garland SM, Tabrizi S. Methods for HPV detection: Polymerase chain reaction assays. In: Monsonego J, editor. Emerging issues on HPV infections: From science to practice. Switzerland: Karger; 2006. pp. 63-72.
[9]Sterling JC, Kurtz JB. Viral infections. In: Champion RH, Burton JL, Burns DA, Breathnach SM. Textbook of Dermatology. 8th Edition. London: Blackwell Science; 1998. pp. 995-1095.
[10]Solomon D, Schiffman M, Tarone R, ALTS Study group. Comparison of three management strategies for patients with atypical squamous cells of undetermined significance: Baseline results from a randomized trial. J Natl Cancer Inst. 2001;93(4):293-9.
[11]Cope JU, Hildesheim A, Schiffman MH, Manos MM, Lörincz AT, Burk RD, et al. Comparison of the hybrid capture tube test and PCR for detection of human papillomavirus DNA in cervical specimens. J Clin Microbiol. 1997;35(9):2262-5.
[12]Nomelini RS, Barcelos AC, Michelin MA, Adad SJ, Murta EF. Utilization of human papillomavirus testing for cervical cancer prevention in a university hospital. Cad Saude Publica. 2007;23(6):1309-18.