@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(2):81-86
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(2):81-86
Petal and Stamen Allergenicity Effect of Old Ontogenical Staged Spartium junceum L. in Guinea Pig
ARTICLE INFO
Article Type
Original ResearchAuthors
Iziy E. (1 )Beheshti Nasr S.M. (* )
Majd A. (2 )
(* ) Cellular and Molecular Biology Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
(1 ) Traditional and Complementary Medicine Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
(2 ) Biology Department, Biological Sciences Faculty, Kharazmi University, Tehran, Iran
Correspondence
Address: Cell & Molecular Research Center, Medicine Faculty, No.2 Building, Sabzevar University of Medical Sciences, Asadabadi Street, Sabzevar, IranPhone: +985714446070 (246)
Fax: +985714445648
beheshti.m1985@gmail.com
Article History
Received: December 13, 2013Accepted: June 7, 2014
ePublished: July 1, 2014
ABSTRACT
Aims
Planting Spartium junceum L. has increased immensely in urban areas due to
its aromatic petals. Since the petals and stamens in aromatic plants are of important
herbal allergenic agents, the aim of this study was to investigate the allergenicity of
petal and stamen at old ontogenical staged Spartium junceum L. in Guinea pig.
Materials & Methods In this experimental study, 21 male Hartley Guinea pigs were randomly divided into three groups including 7 animals. To the first group buffered phosphate saline, second group old petal extract and the third group petal and stamen extract were injected. The extracts were prepared with 16% concentration. The injections were continued within 4 weeks, once per week intraperitoneally and in the fifth week subcutaneous injection was performed. A week after the last injection blood sampling was done from the heart of animals and the number of Eosinophils, Immunoglobulin E and blood sugar levels were measured. To data analysis SPSS 16 software, ANOVA and dependent T tests were used.
Findings In skin test wheal diameter in both groups treated with Spartium junceum L. significantly increased compared to control group (p<0.001). Blood sugar in groups treated with petal and stamen showed significant increase in comparison with control group (p<0.05). In electrophoretic profiles 3 protein bands was observed in the range of 46 to 85kD in both treated groups which these bands were much more colorful in petal and stamen group.
Conclusion Allergenicity of petal with stamen of Spartium junceum L. at old ontogenical stage is more than petal.
Materials & Methods In this experimental study, 21 male Hartley Guinea pigs were randomly divided into three groups including 7 animals. To the first group buffered phosphate saline, second group old petal extract and the third group petal and stamen extract were injected. The extracts were prepared with 16% concentration. The injections were continued within 4 weeks, once per week intraperitoneally and in the fifth week subcutaneous injection was performed. A week after the last injection blood sampling was done from the heart of animals and the number of Eosinophils, Immunoglobulin E and blood sugar levels were measured. To data analysis SPSS 16 software, ANOVA and dependent T tests were used.
Findings In skin test wheal diameter in both groups treated with Spartium junceum L. significantly increased compared to control group (p<0.001). Blood sugar in groups treated with petal and stamen showed significant increase in comparison with control group (p<0.05). In electrophoretic profiles 3 protein bands was observed in the range of 46 to 85kD in both treated groups which these bands were much more colorful in petal and stamen group.
Conclusion Allergenicity of petal with stamen of Spartium junceum L. at old ontogenical stage is more than petal.
CITATION LINKS
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[2]Majd A, Rezanezhad F, Moein M, Aminzadeh M, Shariatzadeh SMA. Air pollution effects on microsporogenesis, pollen development and pollen soluble in Spartium junceum L. (Fabaceae). Pajouhesh-va- Sazandegi. 2004;16(61):10-7. [Persian]
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[5]Amjad L, Amjad A, Fallahian F, Saadatmand S. Comparative study of allergenicity of mature and immature pollen grains of Achillea wilhelmsii. Arak Med Univ J. 2008;11(2):1-9. [Persian]
[6]Garcia-Casado G, Sanchez-Monge R, Chrispeels MJ, Armentia A, Salcedo G, Gomez L. Role of complex asparagine-linked glycans in the allergenicity of plant glycoproteins. Glycobiology. 1996;6(4):471-7.
[7]Sampedro J, Cosgrove DJ. The expansin superfamily. Genome Biol. 2005;6(12):1-11.
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[9]Greinwald R, Lurz G, Witte L, Czygan FC. A survey of alkaloids in Spartium junceum L. (Genisteae-Fabaceae). Zeitschrift für Naturforschung C. 1990;45(11-12):1085-9.
[10]Knox RB, Suphioglu C. Pollen allergens: Development and function. Sex Plant Reprod. 1996;9(6):318-23.
[11]Yamasaki S, Saito T. Regulation of mast cell activation through FcepsilonRI. Chem Immunol Allergy. 2005;87:22- 31.
[12]Solley GO, Gleich GJ, Jordon RE, Schroeter AL. The late phase of the immediate wheal and flare skin reaction. Its dependence upon IgE antibodies. J Clin Invest. 1976;58(2):408-20.
[13]Smith TF. Allergy testing in clinical practice. Ann Allergy. 1992;68(4):293-301.
[14]Rezanezhad F, Chehregani A. Allergenicity and identification of specific IgE binding proteins in pollen of Spartium junceum L. (Fabaceae) and Lagerstroemia indica L. (Lytrace): The effect of air pollution on their allergenecity. Iran J Sci Technol Trans A. 2008;32(A2):129- 34.
[15]Izi E, Beheshti Nasr SM, Majid A, Mohammadzadeh M. Study of allergenicity of petal and stamen in middle-aged ontogenical stage of Spartium Junceum L. in guinea pig. J Sabzevar Univ Med Sci. 2013;20(2):176-83. [Persian]
[16]Miyauchi H, Horio T. A new animal model for contact dermatitis: The hairless guinea pig. J Dermatol. 1992;19(3):140-5.
[17]Moon KC, Wester RC, Maibach HI. Diseased skin models in the hairless guinea pig: In vivo percutaneous absorption. Dermatology. 1990;180(1):8-12.
[18]Prakashkumar R, Mathew PM, Ravindran P. Studies on the allergenicity of nine tropical pollen allergens. Grana. 1998;37(3):185-8.
[19]Chehregani A, Majde A, Moin M, Gholami M, Shariatzadeh MA, Nassiri H. Increasing allergy potency of Zinnia pollen grains in polluted areas. Ecotoxicol Environ Saf. 2004;58(2):267-72.
[20]Stokes JR, Hartel R, Ford LB, Casale TB. Cannabis (hemp) positive skin tests and respiratory symptoms. Ann Allergy Asthma Immunol. 2000;85(3):238-40.
[21]Zanganeh Nasseri M, Majd A, Tajadod G. Study of anatomical structure of vegetative and reproductive organs, development of pollen grains and pollen allergenicity of Caesalpinia giliesii. J Sci Islam Azad Univ. 2010;19(74/1):75-82.
[22]Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[23]Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109(Suppl 1):69-75.
[24]Wink M, Hartmann T, Witte L, Rheinheimer J. Interrelationship between quinolizidine alkaloid producing legumes and infesting insects: exploitation of the alkaloidcontaining phloem sap of Cytisus scoparius by the broom aphid Aphis cytisorum. Z Naturforsch. 1982;37:1081-6.
[25]Wink M, Witte L. Storage of quinolizidine alkaloids in Macrosiphum albifrons and Aphis genistae (Homoptera: Aphididae). Entomol Gen. 1991;15(4):237-54.
[26]Eleno N, Botana L, Espinosa J. K-channel blocking drugs induce histamine release and 45Ca uptake in isolated mast cells. Int Arch Allergy Appl Immunol. 1990;92(2):162- 7.
[27]Sullivan TJ. Antigen-specific desensitization of patients allergic to penicillin. J Allergy Clin Immunol. 1982;69(6):500-8.
[28]Davies JM, Bright ML, Rolland JM, O'Hehir RE. Bahia grass pollen specific IgE is common in seasonal rhinitis patients but has limited cross-reactivity with Ryegrass. Allergy. 2005;60(2):251-5.
[29]Fischer R, McGhee JR, Vu HL, Atkinson TP, Jackson RJ, Tome D, et al. Oral and nasal sensitization promote distinct immune responses and lung reactivity in a mouse model of peanut allergy. Am J Pathol. 2005;167(6):1621-30.
[30]Radauer C, Willerroider M, Fuchs H, Hoffmann- Sommergruber K, Thalhamer J, Ferreira F, et al. Crossreactive and species-specific immunoglobulin E epitopes of plant profilins: An experimental and structure-based analysis. Clin Exp Allergy. 2006;36(7):920-9.
[31]Chalabian F, Mansouri M, Sharifnia F. The Study of ultrastructure features, allergenicity and influence of air pollution on allergenicity of mature pollens in Cercis siliquastrun. Biol J. 2009;4(1):2-8.
[32]Singh AB, Malik P, Parkash D, Gangal SV. Identification of specific IgE binding proteins in Castor bean (Ricinus communis) pollen obtained from different source materials. Grana. 1993;32(6):376-80.
[2]Majd A, Rezanezhad F, Moein M, Aminzadeh M, Shariatzadeh SMA. Air pollution effects on microsporogenesis, pollen development and pollen soluble in Spartium junceum L. (Fabaceae). Pajouhesh-va- Sazandegi. 2004;16(61):10-7. [Persian]
[3]Peterson DJ, Prasad R. The biology of Canadian weeds. 109. Cytisus scoparius (L.) link. Can J Plant Sci. 1998;78(3):497-504.
[4]Srinivasan MP, Shenoy K, Gleeson SK. Population structure of Scotch broom (Cytisus scoparius) and its invasion impacts on the resident plant community in the grasslands of Nilgiris, India. Curr Sci. 2007;93(8):1108-13.
[5]Amjad L, Amjad A, Fallahian F, Saadatmand S. Comparative study of allergenicity of mature and immature pollen grains of Achillea wilhelmsii. Arak Med Univ J. 2008;11(2):1-9. [Persian]
[6]Garcia-Casado G, Sanchez-Monge R, Chrispeels MJ, Armentia A, Salcedo G, Gomez L. Role of complex asparagine-linked glycans in the allergenicity of plant glycoproteins. Glycobiology. 1996;6(4):471-7.
[7]Sampedro J, Cosgrove DJ. The expansin superfamily. Genome Biol. 2005;6(12):1-11.
[8]Yesilada E, Tsuchiya K, Takaishi Y, Kawazoe K. Isolation and characterization of free radical scavenging flavonoid glycosides from the flowers of Spartium junceum by activity-guided fractionation. J Ethnopharmacol. 2000;73(3):471-8.
[9]Greinwald R, Lurz G, Witte L, Czygan FC. A survey of alkaloids in Spartium junceum L. (Genisteae-Fabaceae). Zeitschrift für Naturforschung C. 1990;45(11-12):1085-9.
[10]Knox RB, Suphioglu C. Pollen allergens: Development and function. Sex Plant Reprod. 1996;9(6):318-23.
[11]Yamasaki S, Saito T. Regulation of mast cell activation through FcepsilonRI. Chem Immunol Allergy. 2005;87:22- 31.
[12]Solley GO, Gleich GJ, Jordon RE, Schroeter AL. The late phase of the immediate wheal and flare skin reaction. Its dependence upon IgE antibodies. J Clin Invest. 1976;58(2):408-20.
[13]Smith TF. Allergy testing in clinical practice. Ann Allergy. 1992;68(4):293-301.
[14]Rezanezhad F, Chehregani A. Allergenicity and identification of specific IgE binding proteins in pollen of Spartium junceum L. (Fabaceae) and Lagerstroemia indica L. (Lytrace): The effect of air pollution on their allergenecity. Iran J Sci Technol Trans A. 2008;32(A2):129- 34.
[15]Izi E, Beheshti Nasr SM, Majid A, Mohammadzadeh M. Study of allergenicity of petal and stamen in middle-aged ontogenical stage of Spartium Junceum L. in guinea pig. J Sabzevar Univ Med Sci. 2013;20(2):176-83. [Persian]
[16]Miyauchi H, Horio T. A new animal model for contact dermatitis: The hairless guinea pig. J Dermatol. 1992;19(3):140-5.
[17]Moon KC, Wester RC, Maibach HI. Diseased skin models in the hairless guinea pig: In vivo percutaneous absorption. Dermatology. 1990;180(1):8-12.
[18]Prakashkumar R, Mathew PM, Ravindran P. Studies on the allergenicity of nine tropical pollen allergens. Grana. 1998;37(3):185-8.
[19]Chehregani A, Majde A, Moin M, Gholami M, Shariatzadeh MA, Nassiri H. Increasing allergy potency of Zinnia pollen grains in polluted areas. Ecotoxicol Environ Saf. 2004;58(2):267-72.
[20]Stokes JR, Hartel R, Ford LB, Casale TB. Cannabis (hemp) positive skin tests and respiratory symptoms. Ann Allergy Asthma Immunol. 2000;85(3):238-40.
[21]Zanganeh Nasseri M, Majd A, Tajadod G. Study of anatomical structure of vegetative and reproductive organs, development of pollen grains and pollen allergenicity of Caesalpinia giliesii. J Sci Islam Azad Univ. 2010;19(74/1):75-82.
[22]Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[23]Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109(Suppl 1):69-75.
[24]Wink M, Hartmann T, Witte L, Rheinheimer J. Interrelationship between quinolizidine alkaloid producing legumes and infesting insects: exploitation of the alkaloidcontaining phloem sap of Cytisus scoparius by the broom aphid Aphis cytisorum. Z Naturforsch. 1982;37:1081-6.
[25]Wink M, Witte L. Storage of quinolizidine alkaloids in Macrosiphum albifrons and Aphis genistae (Homoptera: Aphididae). Entomol Gen. 1991;15(4):237-54.
[26]Eleno N, Botana L, Espinosa J. K-channel blocking drugs induce histamine release and 45Ca uptake in isolated mast cells. Int Arch Allergy Appl Immunol. 1990;92(2):162- 7.
[27]Sullivan TJ. Antigen-specific desensitization of patients allergic to penicillin. J Allergy Clin Immunol. 1982;69(6):500-8.
[28]Davies JM, Bright ML, Rolland JM, O'Hehir RE. Bahia grass pollen specific IgE is common in seasonal rhinitis patients but has limited cross-reactivity with Ryegrass. Allergy. 2005;60(2):251-5.
[29]Fischer R, McGhee JR, Vu HL, Atkinson TP, Jackson RJ, Tome D, et al. Oral and nasal sensitization promote distinct immune responses and lung reactivity in a mouse model of peanut allergy. Am J Pathol. 2005;167(6):1621-30.
[30]Radauer C, Willerroider M, Fuchs H, Hoffmann- Sommergruber K, Thalhamer J, Ferreira F, et al. Crossreactive and species-specific immunoglobulin E epitopes of plant profilins: An experimental and structure-based analysis. Clin Exp Allergy. 2006;36(7):920-9.
[31]Chalabian F, Mansouri M, Sharifnia F. The Study of ultrastructure features, allergenicity and influence of air pollution on allergenicity of mature pollens in Cercis siliquastrun. Biol J. 2009;4(1):2-8.
[32]Singh AB, Malik P, Parkash D, Gangal SV. Identification of specific IgE binding proteins in Castor bean (Ricinus communis) pollen obtained from different source materials. Grana. 1993;32(6):376-80.