@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(2):101-107
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(2):101-107
Histochemical Lectin Study of Glycoconjugates Terminal Sugars during Retina Ganglionic Cell Differentiation in Rat Eye
ARTICLE INFO
Article Type
Original ResearchAuthors
Ebrahimi V. (1)Vojoudi E. (1 )
Fazel A.R. (1 )
Ebrahimzadeh Bideskan A.R. (*)
(*) Anatomy & Cell biology Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(1) Anatomy & Cell biology Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
Correspondence
Address: Anatomy & Cell Biology Department, Medicine Faculty, Vakilabad Bolevard, Azadi Square, Mashhad, Iran. Postal Code: 91779-48564Phone: +985118828572
Fax: +985118002484
ebrahimzadehba@mums.ac.ir
Article History
Received: December 16, 2013Accepted: June 15, 2014
ePublished: July 1, 2014
ABSTRACT
Aims
Development of organs during embryonic period is the result of complex
events and changes such as cellular reactions and molecular differentiations of every
dependent structure that determine the ultimate fate of an embryonic
undifferentiated cell. The aim of this study was to investigate the distribution of
glycoconjugates terminal sugars and their changes on ganglionic cells surface of
rat’s retina using histochemichal lectin technique during eye morphogenesis.
Materials & Methods Wistar rats were used. Zero day of pregnancy was determined. Pregnant rats were anesthetized on 14th, 15th and 16th days of gestation and the fetuses were removed and fixed in formalin solution which after tissue passage and 5μ sections preparation exposed to GSA1-B4, DBA, OFA and MPA lectins. Samples color background was Alcian blue with pH equal 2.5.
Findings 14-day samples ganglionic cells showed a poor reaction to the DBA and GSA1-B4 lectins. No reaction was observed on 15th and 16th days with the mentioned lectins. Response of these cells to MPA lectin began from 14th day weakly, increased on 15th day sharply and decreased on 16th day (average response). Reaction of the mentioned cells was moderate with OFA lectin on 14th day, was intense on 15th day and was weak 16th day.
Conclusion Changes in glycoconjugates terminal sugars of cell surface in terms of time is a set process in ganglion cells regulations during eye development and glycoconjugates containing fucose, α-D-Gal, β-D-Gal and N-acetylgalactoseamin terminal sugars play a crucial role in eye retina ganglionic cells development.
Materials & Methods Wistar rats were used. Zero day of pregnancy was determined. Pregnant rats were anesthetized on 14th, 15th and 16th days of gestation and the fetuses were removed and fixed in formalin solution which after tissue passage and 5μ sections preparation exposed to GSA1-B4, DBA, OFA and MPA lectins. Samples color background was Alcian blue with pH equal 2.5.
Findings 14-day samples ganglionic cells showed a poor reaction to the DBA and GSA1-B4 lectins. No reaction was observed on 15th and 16th days with the mentioned lectins. Response of these cells to MPA lectin began from 14th day weakly, increased on 15th day sharply and decreased on 16th day (average response). Reaction of the mentioned cells was moderate with OFA lectin on 14th day, was intense on 15th day and was weak 16th day.
Conclusion Changes in glycoconjugates terminal sugars of cell surface in terms of time is a set process in ganglion cells regulations during eye development and glycoconjugates containing fucose, α-D-Gal, β-D-Gal and N-acetylgalactoseamin terminal sugars play a crucial role in eye retina ganglionic cells development.
CITATION LINKS
[1]Arab MR, Talaei Khouzani T, Fazel AR. Histochemistry and lectin histochemistry of the sclera and choroid in development of the eyes. J Mazandaran Univ Med Sci. 2002;12(34):1-8. [Persian]
[2]Dalby MJ, Gadegaard N, Tare R, Andar A, Riehle MO, Herzyk P, et al. The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat Mater. 2007;6(12):997-1003.
[3]Chung S, Sudo R, Vickerman V, Zervantonakis IK, Kamm RD. Microfluidic platforms for studies of angiogenesis, cell migration, and cell-cell interactions. Ann Biomed Eng. 2010;38(3):1164-77.
[4]Zagris N. Extracellular matrix in development of the early embryo. Micron. 2000;32(4):427-38.
[5]Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, et al. Genomic analysis of mouse retinal development. PLOS Biol. 2004;2(9):247-68.
[6]Cho EYP, Choi HL, Chan FL. Expression pattern of glycoconjugates in rat retina as analysed by lectin histochemistry. Histochem J. 2002;34(11-12):589-600.
[7]Aly K, Salem-Bekhit MM. Histochemical mapping of glycoconjugates in the eyeball of the one humped Camel (Camelus dromedarius). J Pharm Biomed Sci. 2012;2(4):33- 46.
[8]Mubarak HJ, Mghamis MM. Comparative lectins histochemical study of vertebrates' retina. Kufa Med J. 2012;15(1):147-54.
[9]Gulati AK, Zalewski AA, Sharma KB, Ogrowsky D, Sohal GS. A Comparison of lectin binding in rat and human peripheral nerve. J Histochem Cytochem. 1986;34(11):1487-93.
[10]Sarthy PV, Curtis BM, Catterall WA. Retrograde labeling, enrichment, and characterization of retinal ganglion cells from the vertebrates. J Neurosci. 1983;3(12):2532-44.
[11]Ebrahimzadeh Bideskan A, Nikmard F, Mohammadzadeh E, Razavi H, Fazel A. Lectinhistochemistry study of Glycoconjugate terminal sugars during Rat kidney development. Horizon Med Sci. 2012;18(1):26-38. [Persian]
[12]Ebrahimzadeh Bideskan A, Hassanzadeh MM, Nikravesh MR, Fazel AR. Lectin histochemical study of vasculogenesis during rat pituitary morphogenesis. Iranian J Basic Med Sci. 2011;14(1):35-41.
[13]Alles AJ, AR Fazel, SS Spicer, Dom RM. Distribution of glycoconjugates in the optic vesicle and optic cup. Anat Embryol. 1990;182(6):611-6.
[14]White JM. ADAMs: Modulators of cell-cell and cellmatrix interactions. Curr Opin Cell Biol. 2003;15(5):598- 606.
[15]Wu AM, Lisowska E, Duk M, Yang Z. Lectins as tools in glycoconjugate research. Glycoconjugate J. 2009;26(8):899-913.
[16]Mertz AF, Che Y, Banerjee S, Goldstein JM, Rosowski KA, Revilla SF, et al. Cadherin-based intercellular adhesions organize epithelial cell-matrix traction forces. Proc Natl Acad Sci. 2013;110(3):842-7.
[17]Bee JA. Glycoconjugates of the avian eye: The development and maturation of the neural retina as visualized by lectin binding. Differentiation. 1982;23(2):128-40.
[18]Lee JH, Park HS, Shin JM, Chun MH, Oh SJ. Nestin expressing progenitor cells during establishment of the neural retina and its vasculature. Anat Cell Biol. 2012;45(1):38-46.
[19]Soderstrom KO. Lectin binding to the human retina. Anat Rec. 1998;220(2):219-23.
[2]Dalby MJ, Gadegaard N, Tare R, Andar A, Riehle MO, Herzyk P, et al. The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat Mater. 2007;6(12):997-1003.
[3]Chung S, Sudo R, Vickerman V, Zervantonakis IK, Kamm RD. Microfluidic platforms for studies of angiogenesis, cell migration, and cell-cell interactions. Ann Biomed Eng. 2010;38(3):1164-77.
[4]Zagris N. Extracellular matrix in development of the early embryo. Micron. 2000;32(4):427-38.
[5]Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, et al. Genomic analysis of mouse retinal development. PLOS Biol. 2004;2(9):247-68.
[6]Cho EYP, Choi HL, Chan FL. Expression pattern of glycoconjugates in rat retina as analysed by lectin histochemistry. Histochem J. 2002;34(11-12):589-600.
[7]Aly K, Salem-Bekhit MM. Histochemical mapping of glycoconjugates in the eyeball of the one humped Camel (Camelus dromedarius). J Pharm Biomed Sci. 2012;2(4):33- 46.
[8]Mubarak HJ, Mghamis MM. Comparative lectins histochemical study of vertebrates' retina. Kufa Med J. 2012;15(1):147-54.
[9]Gulati AK, Zalewski AA, Sharma KB, Ogrowsky D, Sohal GS. A Comparison of lectin binding in rat and human peripheral nerve. J Histochem Cytochem. 1986;34(11):1487-93.
[10]Sarthy PV, Curtis BM, Catterall WA. Retrograde labeling, enrichment, and characterization of retinal ganglion cells from the vertebrates. J Neurosci. 1983;3(12):2532-44.
[11]Ebrahimzadeh Bideskan A, Nikmard F, Mohammadzadeh E, Razavi H, Fazel A. Lectinhistochemistry study of Glycoconjugate terminal sugars during Rat kidney development. Horizon Med Sci. 2012;18(1):26-38. [Persian]
[12]Ebrahimzadeh Bideskan A, Hassanzadeh MM, Nikravesh MR, Fazel AR. Lectin histochemical study of vasculogenesis during rat pituitary morphogenesis. Iranian J Basic Med Sci. 2011;14(1):35-41.
[13]Alles AJ, AR Fazel, SS Spicer, Dom RM. Distribution of glycoconjugates in the optic vesicle and optic cup. Anat Embryol. 1990;182(6):611-6.
[14]White JM. ADAMs: Modulators of cell-cell and cellmatrix interactions. Curr Opin Cell Biol. 2003;15(5):598- 606.
[15]Wu AM, Lisowska E, Duk M, Yang Z. Lectins as tools in glycoconjugate research. Glycoconjugate J. 2009;26(8):899-913.
[16]Mertz AF, Che Y, Banerjee S, Goldstein JM, Rosowski KA, Revilla SF, et al. Cadherin-based intercellular adhesions organize epithelial cell-matrix traction forces. Proc Natl Acad Sci. 2013;110(3):842-7.
[17]Bee JA. Glycoconjugates of the avian eye: The development and maturation of the neural retina as visualized by lectin binding. Differentiation. 1982;23(2):128-40.
[18]Lee JH, Park HS, Shin JM, Chun MH, Oh SJ. Nestin expressing progenitor cells during establishment of the neural retina and its vasculature. Anat Cell Biol. 2012;45(1):38-46.
[19]Soderstrom KO. Lectin binding to the human retina. Anat Rec. 1998;220(2):219-23.