ARTICLE INFO

Article Type

Original Research

Authors

Jalili   Sh. (*)
Shirzad   H. (1)






(*) Research Institute of Police Science and Social Studies, Tehran, Iran
(1) Research Institute of Police Science and Social Studies, Tehran, Iran

Correspondence

Address: -
Phone: -
Fax: -
jalili.shirin@yahoo.com

Article History

Received:   June  19, 2018
Accepted:   September 17, 2018
ePublished:   September 29, 2018

ABSTRACT

Aims DNA Extraction from forensic samples is the first and one of the most crucial steps of any genetic forensic tests. Hence, obtaining a suitable protocol of DNA extraction that is easy to extract, cheap, time-consuming, and qualified is essential in the forensic genetic research. The present study was carried out with the aim of achieving a suitable, faster, and low-cost method of DNA extraction from different sample for further molecular study in criminal sciences and forensic application.
Materials & Methods After preparation of samples, 3 different DNA extraction methods, including chelex, non-enzymatic, and modified rapid alkaline lysis were evaluated. In order to compare the above-mentioned methods for evaluation of being easy to extract, low-cost, and tim-consuming, the quality and quantity of DNA obtained from each method considering OD, DNA concentration, and PCR efficiency were investigated.
Findings The common 16S rRNA fragment was replicated in all tested samples.
Conclusion Given the ease of access to materials used in each of these methods, as well as their cost-effectiveness in terms of time and cost, these methods can be an appropriate alternative to DNA extraction kits.

Keywords: DNA Extraction, Chelex , Non-enzymatic , Forensic , Modified Rapid Alkaline ,

CITATION LINKS

[1]tray JE, Liu JY, Brevnov MG, Shewale JG. Extraction of DNA from forensic biological samples for genotyping. Forensic Sci Rev. 2010;22(2):159.
[2]Willard JM, Lee DA, Holland MM. Recovery of DNA for PCR amplification from blood and forensic samples using a chelating resin. Methods Mol Biol. 1998;98:9-18.
[3]Stray JE, Shewale JG. Extraction of DNA from human remains. Forensic Sci Rev. 2010;22(2):177-85.
[4] Delefosse T, Hienne R. Extracting DNA from all forensic samples with KingFisher® technologies. Int Congr Ser. 2004;1261:577-9.
[5]Alaeddini R, Walsh SJ, Abbas A. Forensic implications of genetic analyses from degraded DNA—a review. Forensic Sci Int Genet. 2010;4(3):148-57.
[6]Suguna S, Nandal D, Kamble S, Bharatha A, Kunkulol R. Genomic DNA isolation from human whole blood samples by non enzymatic salting out method. Int J Pharm Pharm Sci. 2014;6(6):198-9.
[7] Chacon-Cortes D, Griffiths LR. Methods for extracting genomic DNA from whole blood samples: Current perspectives. J Biorepository Sci Appl Med. 2014;2014(2):1-9.
[8] Madhad VJ, Sentheil KP. The Rapid & Non-Enzymatic isolation of DNA from the Human peripheral whole blood suitable for Genotyping. Eur J Biotechnol Biosci. 2013;1(3):1-16.
[9] Maurya R, Kumar B, Sundar S. Evaluation of salt-out method for the isolation of DNA from whole blood: A pathological approach of DNA based diagnosis. Int J Life Sci Biotechnol Pharma Res. 2013;2(2).
[10]Guha P, Das A, Dutta S, Chaudhuri TK. A rapid and efficient DNA extraction protocol from fresh and frozen human blood samples. J Clin Lab Anal. 2018;32(1):e22181.
[11]Shaik M, Shivanna DK, Kamate M, Ab V, Tp KV. Single lysis-salting out method of genomic DNA extraction from dried blood spots. J Clin Lab Anal. 2016;30(6):1009-12.
[12]Noguera NI, Tallano CE, Bragós IM, Milani AC. Modified salting-out method for DNA isolation from newborn cord blood nucleated cells. J Clin Lab Anal. 2000;14(6):280-3.
[13]Gaaib JN, Nassief AF, Al-Assi AH. Simple salting–out method for genomic DNA extraction from whole blood. Tikrit J Pure Sci. 2011;16(2):9-11.
[14]Nasiri H, Forouzandeh M, Rasaee MJ, Rahbarizadeh F. Modified salting-out method: High-yield, high-quality genomic DNA extraction from whole blood using laundry detergent. J Clin Lab Anal. 2005;19(6):229-32.
[15]Walsh PS, Metzger DA, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques. 1991;10(4):506-13.
[16]Butler JM. DNA extraction from forensic samples using chelex. Cold Spring Harb Protoc. 2009;2009(6):pdb.prot5229.
[17]Im CB, Lee JK, Kim JW, Kwon KC, Koo AH, Park JW, et al. A study of the DNA extraction from bloodstain samples using chelex 100. Korean J Clin Pathol. 1993;13(2):311-20. [Korean]
[18]Rudbeck L, Dissing J. Rapid, simple alkaline extraction of human genomic DNA from whole blood, buccal epithelial cells, semen and forensic stains for PCR. Biotechniques. 1998;25(4):588-90.
[19]Klintschar M, Neuhuber F. Evaluation of an alkaline lysis method for the extraction of DNA from whole blood and forensic stains for STR analysis. J Forensic Sci. 2000;45(3):669-73.
[20]Berthomieu P, Meyer C. Direct amplification of plant genomic DNA from leaf and root pieces using PCR. Plant Mol Biol. 1991;17(3):555-7.
[21]Jeanpierre M. A rapid method for the purification of DNA from blood. Nucleic Acids Res. 1987;15(22):9611.
[22]Loreille OM, Diegoli TM, Irwin JA, Coble MD, Parsons TJ. High efficiency DNA extraction from bone by total demineralization. Forensic Sci Int Genet. 2007;1(2):191-5.
[23]Ivanova NV, Dewaard JR, Hebert PD. An inexpensive, automation-friendly protocol for recovering high-quality DNA. Mol Ecol Resour. 2006;6(4):998-1002.
[24]Brevnov MG, Pawar HS, Mundt J, Calandro LM, Furtado MR, Shewale JG. Developmental validation of the PrepFilerTM forensic DNA extraction kit for extraction of genomic DNA from biological samples. J Forensic Sci. 2009;54(3):599-607.
[25]T. Kitayama, Ogawa Y, Fujii K, Nakahara H, Mizuno N, Sekiguchi K, et al. Evaluation of a new experimental kit for the extraction of DNA from bones and teeth using a non-powder method. Leg Med (Tokyo). 2010;12(2):84-9.