@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2019;25(1):16-21
ISSN: 2252-0805 The Horizon of Medical Sciences 2019;25(1):16-21
The effect of Hydro-Alcoholic Extract of Plantago Major on Indomethacin-Induced Gastric Ulcer in Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Paseban M. (1)Mousavi Fayzabadi V. (2 )
Tayyebi Meibodi N. (3)
Yousefi M. (4)
Hosseini A. (5)
Rakhshandeh H. (*)
(*) Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
(1) Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
(2 ) Department of Biochemistry, Faculty of Basic Science, Mashhad University of Payam Noor, Mashhad, Iran
(3) Department of Pathology, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad , Iran
(4) Department of Persian Medicine, School of Persian and Complementary Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
(5) Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
Correspondence
Address: Pharmacology Department, Faculty of Medicine, Ferdowsi University campus, Azadi Square, Mashhad, IranPhone: +9155036929
Fax: 05138828565
RakhshandehH@mums.ac.ir
Article History
Received: February 25, 2018Accepted: September 22, 2018
ePublished: January 27, 2019
ABSTRACT
Aims
Plantago major is used in traditional medicine for the treatment of gastrointestinal
disorders. The purpose of this study was to examine the effect of hydro – alcoholic extract of
Plantago major on indomethacin-induced gastric ulcer in rats
Materials & Methods 72 male Wistar rats were used in this experimental study. In treatment groups, animals received indomethacin (35 mg/kg) as single dose for induces stomach ulcer, then rats were gavaged by ranitidine (50 mg/kg) and different doses of the extract (200 mg/kg, 400 mg/kg and 800 mg/kg) for 3 days. In preventive groups, animals were treated by ranitidine and different doses of extract for 3 days, and then indomethacin was gavaged on 4th day for stomach ulcer induction. Gastric mucosal damage and acid secretion were evaluated 6 hours later. Statistical analysis was performed using one-way ANOVA.
Findings Acid secretion significantly decreased in therapeutic group that received 200 mg/kg dose of the extract and in preventive groups that received different doses of the extract (p<0.05). Also macroscopic results showed all doses of extract in preventive groups and 200 mg/kg and 800 mg/kg doses of extract in therapeutic groups significantly decreased ulcer index (p<0.05, p<0.01).
Conclusion Hydro – alcoholic extract of Plantago major can protect the gastric mucosa against indomethacin-induced gastric ulcer, via decreasing of gastric acid secretion.
Materials & Methods 72 male Wistar rats were used in this experimental study. In treatment groups, animals received indomethacin (35 mg/kg) as single dose for induces stomach ulcer, then rats were gavaged by ranitidine (50 mg/kg) and different doses of the extract (200 mg/kg, 400 mg/kg and 800 mg/kg) for 3 days. In preventive groups, animals were treated by ranitidine and different doses of extract for 3 days, and then indomethacin was gavaged on 4th day for stomach ulcer induction. Gastric mucosal damage and acid secretion were evaluated 6 hours later. Statistical analysis was performed using one-way ANOVA.
Findings Acid secretion significantly decreased in therapeutic group that received 200 mg/kg dose of the extract and in preventive groups that received different doses of the extract (p<0.05). Also macroscopic results showed all doses of extract in preventive groups and 200 mg/kg and 800 mg/kg doses of extract in therapeutic groups significantly decreased ulcer index (p<0.05, p<0.01).
Conclusion Hydro – alcoholic extract of Plantago major can protect the gastric mucosa against indomethacin-induced gastric ulcer, via decreasing of gastric acid secretion.
CITATION LINKS
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[8]Alkofahi AS, Abdul-Razzak KK, Alzoubi KH, Khabour OF. Report-screening of the anti-hyperglycemic activity of some medicinal plants of Jordan. Pak J Pharm Sci. 2017;30(3):907-12.
[9]Beara IN, Lesjak MM, Jovin ED, Balog KJ, Anackov GT, Orcić DZ, et al. Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. J Agric Food Chem. 2009;57(19):9268-73.
[10]Velasco-Lezama R, Tapia-Aguilar R, Román-Ramos R, Vega-Avila E, Pérez-Gutiérrez MS. Effect of Plantago major on cell proliferation in vitro. J Ethnopharmacol. 2006;103(1):36-42.
[11]Sharifa AA, Neoh YL, Iswadi MI, Khairul O, Abdul Halim M, Jamaludin M, et al. Effects of methanol, ethanol and aqueous extract of Plantago major on gram positive bacteria, gram negative bacteria and yeast. Ann Microsc. 2008;8:42-4.
[12]Beara IN, Orčić DZ, Lesjak MM, Mimica-Dukić NM, Peković BA, Popović MR. Liquid chromatography/tandem mass spectrometry study of anti-inflammatory activity of Plantain (Plantago L.) species. J Pharm Biomed Anal. 2010;52(5):701-6.
[13]Samuelsen AB. The traditional uses, chemical constituents and biological activities of Plantago major L, a review. J Ethnopharmacol. 2000;71(1-2):1-21.
[14]Nyunt TM, Lwin KK, Aye TT, Than MA, Chit K, Kyaw T, et al. Antihypertensive effect of Plantago major Linn, whole plant (ahkyawpaung-tahtaung) on mild to moderate hypertensive patients. Health Sci Res J. 2007;(19):97-102.
[15]Chiang LC, Chiang W, Chang MY, Lin CC. In vitro cytotoxic, antiviral and immunomodulatory effects of Plantago major and Plantago asiatica. Am J Chin Med. 2003;31(2):225-34.
[16]Kobeasy M, Abdel-Fatah OM, Abd El-Salam SM, Mohamed ZM. Gastroprotective effect of Plantago major L. against gastric injury induced by aspirin in rats. J Chem Acta. 2013;2(2):86-91.
[17]Esmaeilian Sh, Mahdavi Shahri N, Fereidoni M, Rakhshandeh H, Niazmand S, Saadatiyan E. The healing effect of lavandula angustifolia hydroalcholic extract on the repair of indomethacin induced gastric ulcer in rats. 13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology; Yazd: Kowsar; 2013.
[18]Melchiorri D, Sewerynek E, Reiter RJ, Ortiz GG, Poeggeler B, Nisticò G. Suppressive effect of melatonin administration on ethanol‐induced gastroduodenal injury in rats in vivo. Br J Pharmacol. 1997;121(2):264-70.
[19]Wallace JL. Prostaglandins, NSAIDs, and gastric mucosal protection: Why doesn't the stomach digest itself?. Physiol Rev. 2008;88(4):1547-65.
[20]Hawkins C, Hanks GW. The gastroduodenal toxicity of nonsteroidal anti-inflammatory drugs. A review of the literature. J Pain Symptom Manage. 2000;20(2):140-51.
[21]Niazmand S, Khooshnood E, Derakhshan M. Effects of Achillea wilhelmsii on rat’s gastric acid output at basal, vagotomized, and vagal-stimulated conditions. Pharmacogn Mag. 2010;6(24):282-5.
[22]Langenbach R, Morham SG, Tiano HF, Loftin CD, Ghanayem BI, Chulada PC, et al. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration. Cell. 1995;83(3):483-92.
[23]Melese E, Asres K, Asad M, Engidawork E. Evaluation of the antipeptic ulcer activity of the leaf extract of Plantago lanceolata L. in rodents. Phytother Res. 2011;25(8):1174-80.
[24]Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: An essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014;94(2):329-54.
[25]Mello JC, Gonzalez MV, Moraes VW, Prieto T, Nascimento OR, Rodrigues T. Protective effect of Plantago major extract against t-BOOH-induced mitochondrial oxidative damage and cytotoxicity. 2015;20(10):17747-59.
[2]Yeomans N, Naesdal J. Systematic review: Ulcer definition in NSAID ulcer prevention trials. Aliment Pharmacol Ther. 2008;27(6):465-72.
[3]Chan FKL. Helicobacter pylori, NSAIDs and gastrointestinal haemorrhage. Eur J Gastroenterol Hepatol. 2002; 14(1):1-3.
[4]Takeuchi K. Pathogenesis of NSAID-induced gastric damage: Importance of cyclooxygenase inhibition and gastric hypermotility. World J Gastroenterol. 2012;18(18):2147-60.
[5]Piwowarski JP, Granica S, Kiss AK. Lythrum Salicaria L. Underestimated medicinal plant from European traditional medicine. A review. J Ethnopharmacol. 2015;170:226-50.
[6]Guo Y, He Y, Guo N, Gao J, Ni Y. Variations of the composition of the leaf cuticular wax among Chinese populations of Plantagomajor. Chem Biodivers. 2015;12(4):627-36.
[7]Atta A, Abo El-Sooud K. The antinociceptive effect of some Egyptian medicinal plant extracts. J Ethnopharmacol. 2004;95(2-3):235-8.
[8]Alkofahi AS, Abdul-Razzak KK, Alzoubi KH, Khabour OF. Report-screening of the anti-hyperglycemic activity of some medicinal plants of Jordan. Pak J Pharm Sci. 2017;30(3):907-12.
[9]Beara IN, Lesjak MM, Jovin ED, Balog KJ, Anackov GT, Orcić DZ, et al. Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. J Agric Food Chem. 2009;57(19):9268-73.
[10]Velasco-Lezama R, Tapia-Aguilar R, Román-Ramos R, Vega-Avila E, Pérez-Gutiérrez MS. Effect of Plantago major on cell proliferation in vitro. J Ethnopharmacol. 2006;103(1):36-42.
[11]Sharifa AA, Neoh YL, Iswadi MI, Khairul O, Abdul Halim M, Jamaludin M, et al. Effects of methanol, ethanol and aqueous extract of Plantago major on gram positive bacteria, gram negative bacteria and yeast. Ann Microsc. 2008;8:42-4.
[12]Beara IN, Orčić DZ, Lesjak MM, Mimica-Dukić NM, Peković BA, Popović MR. Liquid chromatography/tandem mass spectrometry study of anti-inflammatory activity of Plantain (Plantago L.) species. J Pharm Biomed Anal. 2010;52(5):701-6.
[13]Samuelsen AB. The traditional uses, chemical constituents and biological activities of Plantago major L, a review. J Ethnopharmacol. 2000;71(1-2):1-21.
[14]Nyunt TM, Lwin KK, Aye TT, Than MA, Chit K, Kyaw T, et al. Antihypertensive effect of Plantago major Linn, whole plant (ahkyawpaung-tahtaung) on mild to moderate hypertensive patients. Health Sci Res J. 2007;(19):97-102.
[15]Chiang LC, Chiang W, Chang MY, Lin CC. In vitro cytotoxic, antiviral and immunomodulatory effects of Plantago major and Plantago asiatica. Am J Chin Med. 2003;31(2):225-34.
[16]Kobeasy M, Abdel-Fatah OM, Abd El-Salam SM, Mohamed ZM. Gastroprotective effect of Plantago major L. against gastric injury induced by aspirin in rats. J Chem Acta. 2013;2(2):86-91.
[17]Esmaeilian Sh, Mahdavi Shahri N, Fereidoni M, Rakhshandeh H, Niazmand S, Saadatiyan E. The healing effect of lavandula angustifolia hydroalcholic extract on the repair of indomethacin induced gastric ulcer in rats. 13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology; Yazd: Kowsar; 2013.
[18]Melchiorri D, Sewerynek E, Reiter RJ, Ortiz GG, Poeggeler B, Nisticò G. Suppressive effect of melatonin administration on ethanol‐induced gastroduodenal injury in rats in vivo. Br J Pharmacol. 1997;121(2):264-70.
[19]Wallace JL. Prostaglandins, NSAIDs, and gastric mucosal protection: Why doesn't the stomach digest itself?. Physiol Rev. 2008;88(4):1547-65.
[20]Hawkins C, Hanks GW. The gastroduodenal toxicity of nonsteroidal anti-inflammatory drugs. A review of the literature. J Pain Symptom Manage. 2000;20(2):140-51.
[21]Niazmand S, Khooshnood E, Derakhshan M. Effects of Achillea wilhelmsii on rat’s gastric acid output at basal, vagotomized, and vagal-stimulated conditions. Pharmacogn Mag. 2010;6(24):282-5.
[22]Langenbach R, Morham SG, Tiano HF, Loftin CD, Ghanayem BI, Chulada PC, et al. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration. Cell. 1995;83(3):483-92.
[23]Melese E, Asres K, Asad M, Engidawork E. Evaluation of the antipeptic ulcer activity of the leaf extract of Plantago lanceolata L. in rodents. Phytother Res. 2011;25(8):1174-80.
[24]Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: An essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014;94(2):329-54.
[25]Mello JC, Gonzalez MV, Moraes VW, Prieto T, Nascimento OR, Rodrigues T. Protective effect of Plantago major extract against t-BOOH-induced mitochondrial oxidative damage and cytotoxicity. 2015;20(10):17747-59.