@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2016;22(2):145-150
ISSN: 2252-0805 The Horizon of Medical Sciences 2016;22(2):145-150
Effect of Ethanolic Extract of Propolis on Serum Biochemical Factors Level and Total Antioxidant Capacity in Adult Male Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Gheybi N. (1)Bakhshi Biniyaz R. (2)
Taherkhani R. (2)
Jahani Hashemi H. (3)
Chegini R. (4)
Saremi M. (4)
Azhdari Zarmehri H. (5)
Najafipour R. (6)
Sofiabadi M. (*)
(*) Physiology Department, Medicine Faculty, Qazvin University of Medical Sciences, Qazvin, Iran
(1) Biomedical Technology Incubator Department, Paramedical School, Qazvin University of Medical Sciences, Qazvin, Iran
(2) Laboratory Sciences Department, Paramedical School, Qazvin University of Medical Sciences, Qazvin, Iran
(3) Biostatics & Social Health Department, Medicine Faculty, Qazvin University of Medical Sciences, Qazvin, Iran
(4) Surgeon Technology Sciences Department, Paramedical School, Qazvin University of Medical Sciences, Qazvin, Iran
(5) Physiology Department, Medicine Faculty, Torbat Haydarieh University of Medical Sciences, Torbat Haydarieh, Iran
(6) Biochemistry & Genetic Department, Medicine Faculty, Qazvin University of Medical Sciences, Qazvin, Iran
Correspondence
Address: Physiology Department, Medicine Faculty, Qazvin University of Medical Sciences, Shahid Bahonar Boulevard, Qazvin, Iran. Postal Code: 3419759811Phone: +982833336001
Fax: +982833324970
mohasofi@yahoo.com
Article History
Received: May 31, 2015Accepted: January 2, 2016
ePublished: March 5, 2016
ABSTRACT
Aims
Propolis is one of the natural materials collected by the honeybees. The material is extensively used to treat some diseases. The aim of this study was to determine the effects of ethanol extract of propolis of Qazvin plain on some biochemical factors, as well as serum antioxidant capacity, in adult male Wistar rats.
Materials & Methods In the experimental study, 40 adult male Wistar rats were divided into four groups (n=10 per group). The first was control group. The experimental groups received 50, 100, and 200mg/kg propolis. After 10-day gavage, the serum of the rats being extracted, glucose, triglyceride, aspartate aminotransferase, alanine aminotransferase, and total antioxidant capacity were measured. Data was analyzed by SPSS 20 software using one-way ANOVA and Tykey’s post-hoc tests.
Findings There was no significant difference in the blood sugar level between different groups and control group. There was a significant reduction in triglyceride level in “100mg/kg” group than control group (p<0.001). There was a significant increase in aspartate aminotransferase level in “100mg/kg” (p<0.01) and “200mg/kg” (p<0.001) groups. There was a significant reduction in alanine aminotransferase level in “50mg/kg” group (p<0.001). Propolis led to a significant increase in the total antioxidant capacity of serum, especially at 50mg/kg (p<0.05) and 100mg/kg (p<0.01) doses.
Conclusion Oral consumption of propolis has a moderating effect on some blood biochemical factors especially triglyceride. In addition, it increases serum total antioxidant capacity. Nevertheless, its excessive consumption might damage patients with liver diseases.
Materials & Methods In the experimental study, 40 adult male Wistar rats were divided into four groups (n=10 per group). The first was control group. The experimental groups received 50, 100, and 200mg/kg propolis. After 10-day gavage, the serum of the rats being extracted, glucose, triglyceride, aspartate aminotransferase, alanine aminotransferase, and total antioxidant capacity were measured. Data was analyzed by SPSS 20 software using one-way ANOVA and Tykey’s post-hoc tests.
Findings There was no significant difference in the blood sugar level between different groups and control group. There was a significant reduction in triglyceride level in “100mg/kg” group than control group (p<0.001). There was a significant increase in aspartate aminotransferase level in “100mg/kg” (p<0.01) and “200mg/kg” (p<0.001) groups. There was a significant reduction in alanine aminotransferase level in “50mg/kg” group (p<0.001). Propolis led to a significant increase in the total antioxidant capacity of serum, especially at 50mg/kg (p<0.05) and 100mg/kg (p<0.01) doses.
Conclusion Oral consumption of propolis has a moderating effect on some blood biochemical factors especially triglyceride. In addition, it increases serum total antioxidant capacity. Nevertheless, its excessive consumption might damage patients with liver diseases.
Keywords:
Propolis,
Antioxidants,
Alanine Aminotransferase ,
Aspartate Aminotransferase ,
Triglycerides ,
Glucose,
CITATION LINKS
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[2]Koya-Miyata S, Arai N, Mizote A, Taniguchi Y, Ushio S, Iwaki K, et al. Propolis prevents diet-induced hyperlipidemia and mitigates weight gain in diet-induced obesity in mice. Biol Pharm Bull. 2009;32(12):2022-8.
[3]Calderón-Montaño JM, Burgos-Morón E, Pérez-Guerrero C, López-Lázaro M. A review on the dietary flavonoid kaempferol. Mini Rev Med Chem. 2011;11(4):298-344.
[4]Sforcin JM, Bankova V. Propolis: is there a potential for the development of new drugs?. J Ethnopharmacol. 2011;133(2):253-60.
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[9]de Figueiredo SM, Nogueira-Machado JA, Almeida Bde M, Abreu SR, de Abreu JA, Filho SA, et al. Immunomodulatory properties of green propolis. Recent Pat Endocr Metab Immune Drug Discov. 2014;8(2):85-94.
[10]Shimizu T, Takeshita Y, Takamori Y, Kai H, Sawamura R, Yoshida H, et al. Efficacy of Brazilian propolis against Herpes Simplex virus type 1 infection in mice and their modes of antiherpetic efficacies. Evid Based Complement Alternat Med. 2011;14:96-104.
[11]Premratanachai P, Chanchao C. Review of the anticancer activities of bee products. Asian Pac J Trop Biomed. 2014;4(5):337-44.
[12]Murad JM, Calvi SA, Soares, Bankova V, Sforcin JM. Effect of propolis from Brazil and Bulgari on fungucidal activity of macrophage against Paracoccidioides brasiliensis. J Ethnopharm. 2002;79(3):331-4.
[13]Feres M, Figueiredo LC, Barreto IM, Coelho MH, Araujo MW, Cortelli SC. In vitro antimicrobial activity of plant extracts and propolis in saliva samples of healthy and periodontally- involved subjects. J Int Acad Periodontol. 2005;7(3):90-6.
[14]Ownagh A, Tukmechi A, Adibhesam M, Ebrahimzadeh S. Comparison study on the effect of ethanol extract of propolis collected from west Azerbaijan against dermatophytes fungi. Urmia Med J. 2010;21(3):206-14.
[15]Momen Beitollahi J, Mansorian A, Esmaili M, Amanlou M, Mohamadnia A, Bahrami N. Antimicrobial effects of propolis extract on the most prevalent oral pathogens: An in vitro study. Majallah-i-dandanpizishki. 2009;21(1):33-9. [Persian]
[16]Fereidooni M, Khosravi Samani M, Amiri A, Seyed M, Haji Ahmadi M. Comparison of the effect of propolis and traditional toothpaste on bacterial plaque. J Babol Univ Med Sci. 2014;16(2):17-22. [Persian]
[17]Velikova M, Bankova V, Tsvetkova I, Kujumgiev A, Marcucci MC. Antibacterial ent-kaurene from Brazilian propolis of native stingless bees. Fitotrapia. 2000;71(6):690-3.
[18]Danert FC, Zampini C, Ordoñez R, Maldonado L, Bedascarrasbure E, Isla MI. Nutritional and functional properties of aqueous and hydroalcoholic extracts from Argentinean propolis. Nat Prod Commun. 2014;9(2):167-70.
[19]Guo X, Chen B, Luo L, Zhang X, Dai X, Gong S. Chemical compositions and antioxidant activities of water extracts of Chinese propolis. J Agric Food Chem. 2011;59(23):12610-6.
[20]Afrouzan H, Tahmasebi Gh, Bankova V, Bigdeli M. Comparison of quantity and quality of propolis produced by gymnosperms and angiosperms plants in northeast of Tehran, Iran. Pajouhesh Sazandegi .2008;77:155-62. [Persian]
[21]Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996;239(1):70-6.
[22]Fernandes FF, Dias AL, Ramos CL, Ikegaki M, de Siqueira AM, Franco MC. The "in vitro" antifungal activity evaluation of propolis G12 ethanol extract on Cryptococcus neoformans. J Rev Inst Med Trop Sao Paulo. 2007;49(2):93-5.
[23]Newairy AS, Salama AF, Hussien HM, Yousef MI. Propolis alleviates aluminium-induced lipid peroxidation and biochemical parameters in male rats. Food Chem Toxicol. 2009;47(6):1093-8.
[24]Eraslan G, Kanbur M, Silici S. Evaluation of propolis evicts on some biochemical parameters in rats treated with sodium Fluoride. Pestic Biochem Physiology. 2007;88(3):273-83.
[25]Khan MS, Devaraj H, Devaraj N. Chrysin abrogates early hepatocarcinogenesis and induces apoptosis in N-nitrosodiethylamine-induced preneoplastic nodules in rats. Toxicol Appl Pharmacol. 2011;251(1):85-94.
[26]Zhu W, Chen M, Shou Q, Li Y, Hu F. Biological activities of chinese propolis and brazilin propolis on streptozotocine induced type1 diabetes mellitus in rats. Evid Based Complement Altern Med. 2011;2011:468529.
[27]Sameni H, Ramhormozi P, Bandegi A, Taherian A, Safari M, Tabriziamjad M. Effects of hydroalcoholic extract of Iranian Propolis on blood serum biochemical factors in streptozotocin-induced diabetic rats. Koomesh. 2014;15(3):388-95. [Persian]
[28]Vaya J, Aviram M. Nutritional antioxidants: Mechanism of action, analyses of activities and medical applications. Curr Med Chem Immun End Metab Age. 2001;1(1):99-117.
[29]Lukačínová A, Mojžiš J, Beňačka R, Keller J, Maguth T, Kurila P, et al. Preventive Effects of Flavonoids on Alloxan-Induced Diabetes Mellitus in Rats. Acta Vet Brno. 2008;77:175-82.
[30]Abo-Salem OM, El-Edel RH, Harisa GE, El-Halawany N, Ghonaim MM. Experimental diabetic nephropathy can be prevented by propolis: effect on metabolic disturbances and renal oxidative parameters. Pak J Pharm Sci. 2009;22(2):205-10.
[31]Sameni H, Kavakebian F, Tabriziamjad M, Bandegi A, Yousefi B, Taherian A. Effects of hydroalcoholic extract of Propolis on oxidative stress indices of rat fetal brain induced by chronic prenatal stress. Koomesh. 2014;15(4):482-92. [Persian]
[32]Sheng J, Zhou J, Wang L, Xu J, Hu Q. Antioxidant activity of ethanol and petroleum ether extracts from Brazilian propolis. Eurn Food Res Technol. 2007;225:249-53.
[33]El-Masry TA, Emara AM, El-Shitany NA. Possible protective effect of propolis against lead induced neurotoxicity in animal model. J Evolut Biol Res. 2011;3(1):4-11.
[34]Seven I, Aksu T, Seven PT. The effects of propolis on biochemical parameters and activity of antioxidant enzymes in broilers exposed to lead-induced oxidative stress. Asian Australas J Anim Sci. 2010;23(11):1482-9.
[2]Koya-Miyata S, Arai N, Mizote A, Taniguchi Y, Ushio S, Iwaki K, et al. Propolis prevents diet-induced hyperlipidemia and mitigates weight gain in diet-induced obesity in mice. Biol Pharm Bull. 2009;32(12):2022-8.
[3]Calderón-Montaño JM, Burgos-Morón E, Pérez-Guerrero C, López-Lázaro M. A review on the dietary flavonoid kaempferol. Mini Rev Med Chem. 2011;11(4):298-344.
[4]Sforcin JM, Bankova V. Propolis: is there a potential for the development of new drugs?. J Ethnopharmacol. 2011;133(2):253-60.
[5]Burdock GA. Review of the biological properties and toxicity of bee propolis. Food Chem Toxicol. 1998;36(4):347-63.
[6]Scifo C, Cardile V, Russo A, Consoli R, Vancheri C, Capasso F, et al. Resveratrol and propolis as necrosis or apoptosis inducers in human prostate carcinoma cells. Oncol Res. 2004;14(9):415-26.
[7]De Vecchi E, Drago L. [Propolis' antimicrobial activity: What's new?]. Infez Med. 2007;15(1):7-15. [Italian]
[8]Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol.1999;64(3):235-40.
[9]de Figueiredo SM, Nogueira-Machado JA, Almeida Bde M, Abreu SR, de Abreu JA, Filho SA, et al. Immunomodulatory properties of green propolis. Recent Pat Endocr Metab Immune Drug Discov. 2014;8(2):85-94.
[10]Shimizu T, Takeshita Y, Takamori Y, Kai H, Sawamura R, Yoshida H, et al. Efficacy of Brazilian propolis against Herpes Simplex virus type 1 infection in mice and their modes of antiherpetic efficacies. Evid Based Complement Alternat Med. 2011;14:96-104.
[11]Premratanachai P, Chanchao C. Review of the anticancer activities of bee products. Asian Pac J Trop Biomed. 2014;4(5):337-44.
[12]Murad JM, Calvi SA, Soares, Bankova V, Sforcin JM. Effect of propolis from Brazil and Bulgari on fungucidal activity of macrophage against Paracoccidioides brasiliensis. J Ethnopharm. 2002;79(3):331-4.
[13]Feres M, Figueiredo LC, Barreto IM, Coelho MH, Araujo MW, Cortelli SC. In vitro antimicrobial activity of plant extracts and propolis in saliva samples of healthy and periodontally- involved subjects. J Int Acad Periodontol. 2005;7(3):90-6.
[14]Ownagh A, Tukmechi A, Adibhesam M, Ebrahimzadeh S. Comparison study on the effect of ethanol extract of propolis collected from west Azerbaijan against dermatophytes fungi. Urmia Med J. 2010;21(3):206-14.
[15]Momen Beitollahi J, Mansorian A, Esmaili M, Amanlou M, Mohamadnia A, Bahrami N. Antimicrobial effects of propolis extract on the most prevalent oral pathogens: An in vitro study. Majallah-i-dandanpizishki. 2009;21(1):33-9. [Persian]
[16]Fereidooni M, Khosravi Samani M, Amiri A, Seyed M, Haji Ahmadi M. Comparison of the effect of propolis and traditional toothpaste on bacterial plaque. J Babol Univ Med Sci. 2014;16(2):17-22. [Persian]
[17]Velikova M, Bankova V, Tsvetkova I, Kujumgiev A, Marcucci MC. Antibacterial ent-kaurene from Brazilian propolis of native stingless bees. Fitotrapia. 2000;71(6):690-3.
[18]Danert FC, Zampini C, Ordoñez R, Maldonado L, Bedascarrasbure E, Isla MI. Nutritional and functional properties of aqueous and hydroalcoholic extracts from Argentinean propolis. Nat Prod Commun. 2014;9(2):167-70.
[19]Guo X, Chen B, Luo L, Zhang X, Dai X, Gong S. Chemical compositions and antioxidant activities of water extracts of Chinese propolis. J Agric Food Chem. 2011;59(23):12610-6.
[20]Afrouzan H, Tahmasebi Gh, Bankova V, Bigdeli M. Comparison of quantity and quality of propolis produced by gymnosperms and angiosperms plants in northeast of Tehran, Iran. Pajouhesh Sazandegi .2008;77:155-62. [Persian]
[21]Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996;239(1):70-6.
[22]Fernandes FF, Dias AL, Ramos CL, Ikegaki M, de Siqueira AM, Franco MC. The "in vitro" antifungal activity evaluation of propolis G12 ethanol extract on Cryptococcus neoformans. J Rev Inst Med Trop Sao Paulo. 2007;49(2):93-5.
[23]Newairy AS, Salama AF, Hussien HM, Yousef MI. Propolis alleviates aluminium-induced lipid peroxidation and biochemical parameters in male rats. Food Chem Toxicol. 2009;47(6):1093-8.
[24]Eraslan G, Kanbur M, Silici S. Evaluation of propolis evicts on some biochemical parameters in rats treated with sodium Fluoride. Pestic Biochem Physiology. 2007;88(3):273-83.
[25]Khan MS, Devaraj H, Devaraj N. Chrysin abrogates early hepatocarcinogenesis and induces apoptosis in N-nitrosodiethylamine-induced preneoplastic nodules in rats. Toxicol Appl Pharmacol. 2011;251(1):85-94.
[26]Zhu W, Chen M, Shou Q, Li Y, Hu F. Biological activities of chinese propolis and brazilin propolis on streptozotocine induced type1 diabetes mellitus in rats. Evid Based Complement Altern Med. 2011;2011:468529.
[27]Sameni H, Ramhormozi P, Bandegi A, Taherian A, Safari M, Tabriziamjad M. Effects of hydroalcoholic extract of Iranian Propolis on blood serum biochemical factors in streptozotocin-induced diabetic rats. Koomesh. 2014;15(3):388-95. [Persian]
[28]Vaya J, Aviram M. Nutritional antioxidants: Mechanism of action, analyses of activities and medical applications. Curr Med Chem Immun End Metab Age. 2001;1(1):99-117.
[29]Lukačínová A, Mojžiš J, Beňačka R, Keller J, Maguth T, Kurila P, et al. Preventive Effects of Flavonoids on Alloxan-Induced Diabetes Mellitus in Rats. Acta Vet Brno. 2008;77:175-82.
[30]Abo-Salem OM, El-Edel RH, Harisa GE, El-Halawany N, Ghonaim MM. Experimental diabetic nephropathy can be prevented by propolis: effect on metabolic disturbances and renal oxidative parameters. Pak J Pharm Sci. 2009;22(2):205-10.
[31]Sameni H, Kavakebian F, Tabriziamjad M, Bandegi A, Yousefi B, Taherian A. Effects of hydroalcoholic extract of Propolis on oxidative stress indices of rat fetal brain induced by chronic prenatal stress. Koomesh. 2014;15(4):482-92. [Persian]
[32]Sheng J, Zhou J, Wang L, Xu J, Hu Q. Antioxidant activity of ethanol and petroleum ether extracts from Brazilian propolis. Eurn Food Res Technol. 2007;225:249-53.
[33]El-Masry TA, Emara AM, El-Shitany NA. Possible protective effect of propolis against lead induced neurotoxicity in animal model. J Evolut Biol Res. 2011;3(1):4-11.
[34]Seven I, Aksu T, Seven PT. The effects of propolis on biochemical parameters and activity of antioxidant enzymes in broilers exposed to lead-induced oxidative stress. Asian Australas J Anim Sci. 2010;23(11):1482-9.