@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(2):109-113
ISSN: 2252-0805 The Horizon of Medical Sciences 2014;20(2):109-113
Effect of Hydro-Alcoholic Extract of Nigella sativa on Gastric Acid and Mucus Secretion in Cyclooxygenase Inhibition Condition in Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Paseban M. (1 )Niazmand S. (*)
Shafei M.N. (1 )
Soukhtanlou M. (2)
(*) Physiology Department, , Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(1 ) Physiology Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(2) Biochemistry Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
Correspondence
Address: Physiology Department, Medicine Faculty, Azadi Square, Mashhad, Khorasan Razavi, Iran. Postal Code: 91779-48564Phone: +985118002223
Fax: +985118828564
niazmands@mums.ac.ir
Article History
Received: November 18, 2013Accepted: May 7, 2014
ePublished: July 1, 2014
ABSTRACT
Aims
In various studies and also in traditional medicine, many properties for
Nigella sativa, such as antioxidant effects and gastric mucosa protection have been
reported. The aim of this study was to investigate the effect of hydro-alcoholic
extract of Nigella sativa on acid and mucus secretion in cyclooxygenase inhibition
conditions.
Materials & Methods This interventional study was done using 40 Wistar rats. The rats were divided into 5 groups; positive control (distilled water), Ranitidine (50mg/kg) and three groups receiving Nigella sativa extract (100, 200 and 400mg/kg). The groups were treated by gavage for 5 days and on 6th day Indomethacin was gavaged to cyclooxygenase inhibition and 6 hours later acid and mucus secretion were examined.
Findings No differences were observed in acid secretion between treated groups with Ranitidine, the extract and Indomethacin. But mucus secretion in groups of 100 and 200mg/kg doses of the extract was higher than the group receiving Indomethacin significantly.
Conclusion Nigella seed extract can protect the gastric mucosa against damages caused by cyclooxygenase inhibition, but is not able to reduce the acid secretion elevated by Indomethacin.
Materials & Methods This interventional study was done using 40 Wistar rats. The rats were divided into 5 groups; positive control (distilled water), Ranitidine (50mg/kg) and three groups receiving Nigella sativa extract (100, 200 and 400mg/kg). The groups were treated by gavage for 5 days and on 6th day Indomethacin was gavaged to cyclooxygenase inhibition and 6 hours later acid and mucus secretion were examined.
Findings No differences were observed in acid secretion between treated groups with Ranitidine, the extract and Indomethacin. But mucus secretion in groups of 100 and 200mg/kg doses of the extract was higher than the group receiving Indomethacin significantly.
Conclusion Nigella seed extract can protect the gastric mucosa against damages caused by cyclooxygenase inhibition, but is not able to reduce the acid secretion elevated by Indomethacin.
CITATION LINKS
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[2]Koda-Kimble AN, Alldredge BK. Applied therapeutics: The clinical use of druges. 10th ed. Baltimore: Wolters Kluwer Health/Lippincott Williams & Wilkins; 1978.
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[6]Barrett KE BS, Barman SM, Boitano S, Brooks H. Ganongs review of medical physiology. 24rd ed. New York: Mc Graw Hill; 2010.
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[9]Hadjzadeh MAR, Mohammadian N, Rahmani Z, Rassouli FB. Effect of thymoquinone on ethylene glycol-induced kidney calculi in rats. Urol J. 2008;5(3):149-55.
[10]Hannan A, Saleem S, Chaudhary S, Barkaat M, Arshad MU. Anti bacterial activity of Nigella sativa against clinical isolates of methicillin resistant Staphylococcus aureus. J Ayub Med Coll Abbottabad. 2008;20(3):72-4.
[11]Al-Naggar TB, Gómez-Serranillos MP, Carretero ME, Villar AM. Neuropharmacological activity of Nigella sativa L. extracts. J Ethnopharmacol. 2003;88(1):63-8.
[12]Al-Hader A, Aqel M, Hasan Z. Hypoglycemic effects of the volatile oil of Nigella sativa. Int J Pharm. 1993;31(2):96-100.
[13]Boskabady MH, Keyhanmanesh R, Ebrahimi Saadatloo MA. Relaxant effects of different fractions from Nigella sativa L. on guinea pig tracheal chains and its possible mechanism(s). Indian J Exp Biol. 2008;46(12):805-10.
[14]Farah N, Benghuzzi H, Tucci M, Cason Z. The effects of isolated antioxidants from black seed on the cellular metabolism of A549 cells. Biomed Sci Instrum. 2004;41:211-6.
[15]Al-Ghamdi MS. The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa. J Ethnopharmacol. 2001;76(1):45-8.
[16]Mahmoud MR, El-Abhar HS, Saleh S. The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. J Ethnopharmacol. 2002;79(1):1-11.
[17]El-Dakhakhny M. Studies on the Egyptian Nigella sativa L. IV Some pharmacological properties of seeds active principle in comparison to its dihydro compound and its polymer. Arzneimittelforschung. 1965;15(10):1227-9.
[18]El-Tahir KE, Ashour M, Al-Harbi MM. The cardiovascular actions of the volatile oil of the black seed (Nigella sativa) in rats: Elucidation of the mechanism of action. Gen Pharm Vasc Sys. 1993;24(5):1123-31.
[19]Corne SJ, Morrissy SM, Wood RJ. Proceedings: A method for the quantitive estimation of gastric barrier mucus. J Physiol 1974;242(2):116-7.
[20]Rao CV, Sairam K, Goel RK. Experimental evaluation of Bacopa monnieraon rat gastric ulceration and secretion. Indian J Physiol Pharmacol. 2000;44(1):35-41.
[21]Naito Y, Yoshikawa T. Oxidative stress involvement and gene expression in indomethacin-induced gastropathy. Redox Rep. 2006;11(6):243-53.
[22]Suleyman H, Albayrak A, Bilici M, Cadirci E, Halici Z. Different mechanisms in formation and prevention of indomethacin-induced gastric ulcers. Inflammation. 2010;33(4):224-34.
[23]Venables C. Mucus, pepsin, and peptic ulcer. Gut. 1986;27(3):233-8.
[24]Wallace J, Whittle BJ. Role of mucus in the repair of gastric epithelial damage in the rat. Inhibition of epithelial recovery by mucolytic agents. Gastroenterology. 1986;91(3):603-11.
[25]Cross C, Halliwell B, Allen A. Antioxidant protection: A function of tracheobronchial and gastrointestinal mucus. Lancet. 1984;323(8390):1328-30.
[26]Bandyopadhyay SK, Pakrashi SC, Pakrashi A. The role of antioxidant activity of "Phyllanthus emblica" fruits on prevention from indomethacin induced gastric ulcer. J Ethnopharmacol. 2000;70(2):171-6.
[27]Hoogerwerf WA, Pasricha PJ. Agents used for the control of gastric acidity and treatment of peptic ulcer and gastrooesophageal reflux diseases. Goodman & Gilman’s The Pharmacological Basis of Therapeutics, 10th ed. New York: Mc Graw-Hill; 2001.
[28]El-Dakhakhny M, Barakat M, El-Halim M, Aly SM. Effects of Nigella sativa oil gastric secretion and ethanolinduced ulcer in rats. J Ethnopharmacol. 2000;72(1):299- 304.
[29]Arroyo M-T, Lanas A, Sáinz R. Prevention and healing of experimental indomethacin-induced gastric lesions: Effects of ebrotidine, omeprazole and ranitidine. Eur J Gastroenterol Hepatol. 2000;12(3):313-8.
[30]Terzi Coelho CB, Dragosavac D, Coelho Neto JS, Montes CG, Guerrazzi F, Andreollo NA. Ranitidine is unable to maintain gastric ulcer pH levels above 4 in septic patients. J Crit Care. 2009;24(4):627.e7-627.e13.
[2]Koda-Kimble AN, Alldredge BK. Applied therapeutics: The clinical use of druges. 10th ed. Baltimore: Wolters Kluwer Health/Lippincott Williams & Wilkins; 1978.
[3]Yeomans ND, Naesdal J. Systematic review: Ulcer definition in NSAID ulcer prevention trials. Aliment Pharm Ther. 2008;27(6):465-72.
[4]Chan FK. Helicobacter pylori, NSAIDs and gastrointestinal haemorrhage. Eur J Gastroenterol Hepatol. 2002;14(1):1-3.
[5]Brzozowski T, Konturek PC, Konturek SJ, Brzozowska I, Pawlik T. Role of prostaglandins in gastroprotection and gastric adaptation. J Physiol Pharmacol. 2005;56(5):33-55.
[6]Barrett KE BS, Barman SM, Boitano S, Brooks H. Ganongs review of medical physiology. 24rd ed. New York: Mc Graw Hill; 2010.
[7]Yoshikawa T, Naito Y, Kishi A, Tomii T, Kaneko T, Iinuma S, et al. Role of active oxygen, lipid peroxidation, and antioxidants in the pathogenesis of gastric mucosal injury induced by indomethacin in rats. Gut. 1993;34(6):732-7.
[8]Mir Heydar H. Plant referrences: Use of plants in prevention and treatment of diseases. Tehran: Farhang-e- Eslami; 2003. [Persian]
[9]Hadjzadeh MAR, Mohammadian N, Rahmani Z, Rassouli FB. Effect of thymoquinone on ethylene glycol-induced kidney calculi in rats. Urol J. 2008;5(3):149-55.
[10]Hannan A, Saleem S, Chaudhary S, Barkaat M, Arshad MU. Anti bacterial activity of Nigella sativa against clinical isolates of methicillin resistant Staphylococcus aureus. J Ayub Med Coll Abbottabad. 2008;20(3):72-4.
[11]Al-Naggar TB, Gómez-Serranillos MP, Carretero ME, Villar AM. Neuropharmacological activity of Nigella sativa L. extracts. J Ethnopharmacol. 2003;88(1):63-8.
[12]Al-Hader A, Aqel M, Hasan Z. Hypoglycemic effects of the volatile oil of Nigella sativa. Int J Pharm. 1993;31(2):96-100.
[13]Boskabady MH, Keyhanmanesh R, Ebrahimi Saadatloo MA. Relaxant effects of different fractions from Nigella sativa L. on guinea pig tracheal chains and its possible mechanism(s). Indian J Exp Biol. 2008;46(12):805-10.
[14]Farah N, Benghuzzi H, Tucci M, Cason Z. The effects of isolated antioxidants from black seed on the cellular metabolism of A549 cells. Biomed Sci Instrum. 2004;41:211-6.
[15]Al-Ghamdi MS. The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa. J Ethnopharmacol. 2001;76(1):45-8.
[16]Mahmoud MR, El-Abhar HS, Saleh S. The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. J Ethnopharmacol. 2002;79(1):1-11.
[17]El-Dakhakhny M. Studies on the Egyptian Nigella sativa L. IV Some pharmacological properties of seeds active principle in comparison to its dihydro compound and its polymer. Arzneimittelforschung. 1965;15(10):1227-9.
[18]El-Tahir KE, Ashour M, Al-Harbi MM. The cardiovascular actions of the volatile oil of the black seed (Nigella sativa) in rats: Elucidation of the mechanism of action. Gen Pharm Vasc Sys. 1993;24(5):1123-31.
[19]Corne SJ, Morrissy SM, Wood RJ. Proceedings: A method for the quantitive estimation of gastric barrier mucus. J Physiol 1974;242(2):116-7.
[20]Rao CV, Sairam K, Goel RK. Experimental evaluation of Bacopa monnieraon rat gastric ulceration and secretion. Indian J Physiol Pharmacol. 2000;44(1):35-41.
[21]Naito Y, Yoshikawa T. Oxidative stress involvement and gene expression in indomethacin-induced gastropathy. Redox Rep. 2006;11(6):243-53.
[22]Suleyman H, Albayrak A, Bilici M, Cadirci E, Halici Z. Different mechanisms in formation and prevention of indomethacin-induced gastric ulcers. Inflammation. 2010;33(4):224-34.
[23]Venables C. Mucus, pepsin, and peptic ulcer. Gut. 1986;27(3):233-8.
[24]Wallace J, Whittle BJ. Role of mucus in the repair of gastric epithelial damage in the rat. Inhibition of epithelial recovery by mucolytic agents. Gastroenterology. 1986;91(3):603-11.
[25]Cross C, Halliwell B, Allen A. Antioxidant protection: A function of tracheobronchial and gastrointestinal mucus. Lancet. 1984;323(8390):1328-30.
[26]Bandyopadhyay SK, Pakrashi SC, Pakrashi A. The role of antioxidant activity of "Phyllanthus emblica" fruits on prevention from indomethacin induced gastric ulcer. J Ethnopharmacol. 2000;70(2):171-6.
[27]Hoogerwerf WA, Pasricha PJ. Agents used for the control of gastric acidity and treatment of peptic ulcer and gastrooesophageal reflux diseases. Goodman & Gilman’s The Pharmacological Basis of Therapeutics, 10th ed. New York: Mc Graw-Hill; 2001.
[28]El-Dakhakhny M, Barakat M, El-Halim M, Aly SM. Effects of Nigella sativa oil gastric secretion and ethanolinduced ulcer in rats. J Ethnopharmacol. 2000;72(1):299- 304.
[29]Arroyo M-T, Lanas A, Sáinz R. Prevention and healing of experimental indomethacin-induced gastric lesions: Effects of ebrotidine, omeprazole and ranitidine. Eur J Gastroenterol Hepatol. 2000;12(3):313-8.
[30]Terzi Coelho CB, Dragosavac D, Coelho Neto JS, Montes CG, Guerrazzi F, Andreollo NA. Ranitidine is unable to maintain gastric ulcer pH levels above 4 in septic patients. J Crit Care. 2009;24(4):627.e7-627.e13.