@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;20(4):263-268
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;20(4):263-268
Effect of Brassica oleracea L. (var. capitata) Hydroalcoholic Extract on Epithelial Layer Healing of Skin in Male Mice
ARTICLE INFO
Article Type
Original ResearchAuthors
Khayatzade J. (*)Barakpour E. (1)
Rakhshande H. (2)
(*) Biology Department, Sciences Faculty, Mashhad Branch, Islamic Azad University, Mashhad, Iran
(1) Biology Department, Sciences Faculty, Mashhad Branch, Islamic Azad University, Mashhad, Iran
(2) Pharmacology Department, Medicine Faculty, Ferdowsi University, Mashhad, Iran
Correspondence
Address: Biology Department, Islamic Azad University, Rahnamaei 26 Street, Mashhad, IranPhone: +985138435050
Fax: +985138435050
j.khayatzadeh@mshdiau.ac.ir
Article History
Received: January 17, 2014Accepted: August 10, 2014
ePublished: February 19, 2015
ABSTRACT
Aims
Wound healing is a complex physiological process involves a series of successive stages
of hemostasis, inflammation, proliferation, and regeneration. This study aimed to investigate
the effect of hydroalcoholic extract of Brassica oleracea L. (var. capitata) leaves in comparison
with Phenytoin on epithelial layer healing and the holes in the skin of mice.
Materials & Methods This study was done on 40 male NIH Mice between 25-30gr. Animals were randomly divided into 4 groups (n=10). Negative control group received saline, positive control group administrated with 1% Phenytoin and treated groups received 10% and 20% hydroalcoholic extract twice a day until closing holes. Macroscopic and microscopic analysis was performed on days 1, 4, 7, 12 and 15. Statistical analysis was done by SPSS 16 software and Tukey test.
Findings A significant reduction was observed in average size of holes in 20% hydroalcoholic extract treated group compared to the negative control 10% extract at day 7 (p=0.001). There was a significant reduction in average size of holes in 20% extract group compared to 10% extract and negative control groups at day 12. (p=0.007). The skin holes in 20% extract group were closed earlier than the other groups. Epithelial thickness was increased in 20% extract group from 7th day the much faster than other groups.
Conclusion Brassica oleracea L. (var. capitata) has the same effect of phenytoin in healing of rapid closure of holes and proliferation of epithelial cells.
Materials & Methods This study was done on 40 male NIH Mice between 25-30gr. Animals were randomly divided into 4 groups (n=10). Negative control group received saline, positive control group administrated with 1% Phenytoin and treated groups received 10% and 20% hydroalcoholic extract twice a day until closing holes. Macroscopic and microscopic analysis was performed on days 1, 4, 7, 12 and 15. Statistical analysis was done by SPSS 16 software and Tukey test.
Findings A significant reduction was observed in average size of holes in 20% hydroalcoholic extract treated group compared to the negative control 10% extract at day 7 (p=0.001). There was a significant reduction in average size of holes in 20% extract group compared to 10% extract and negative control groups at day 12. (p=0.007). The skin holes in 20% extract group were closed earlier than the other groups. Epithelial thickness was increased in 20% extract group from 7th day the much faster than other groups.
Conclusion Brassica oleracea L. (var. capitata) has the same effect of phenytoin in healing of rapid closure of holes and proliferation of epithelial cells.
CITATION LINKS
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[8]Lin JY, Li CY, Hwang IF. Characterisation of the pigment components in red cabbage (Brassica oleracea L. var.) juice and their anti-inflammatory effects on LPS-stimulated murine splenocytes. Food Chem. 2008;109(4):771-81.
[9]Burns JL, Mancoll JS, Phillips LG. Impairments to wound healing. Clinics Plast Surg. 2003;30(1):47-56.
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[11]Ruiz P.A, Braune A, Holzlwimmer G, Qu uintanilla-Fend L, Haller D. Quercetin inhibits TNF-induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells. J Nutr. 2007;137(5):1208-15.
[12]Jia Y, Zhao G, Jia J. Preliminary evaluation: the effects of Aloe Ferox Miller and Aleo arborescens Miller in wound healing. J Ethnopharmacol. 2008;120(2):181-9.
[13]Suguna L, Sivakumar P, Chandrakasan G. Effects of Centella asiaticaextract on dermal wound healing in rats. Indian J Exp Biol. 1996;34(12):1208-11.
[14]Paiva LA, de Alencar Cunha KM, Santos FA, Gramosa NV, Silveira ER, Rao VS. Investigation on the wound healing activity of oleoresin from copaifera langsdorffi in rats. Phytother Res. 2002;16(8):737-9.
[15]Townsend Jr CM, Beauchamp RD, Evers MD BM, Mattox MD L. Sabiston textbook of surgery: The biological basis of modern surgical practice. 19th ed. New York: Saunders; 2012.
[16]Krishnan P. The scientific study of herbal wound healing therapies: Current state of play. Curr Anaesth Crit Care. 2009;17(1-2):21-7.
[17]Chithra P, Sajithlal GB, Chandrakasan G. Influence of Aloe vera on the glycosaminoglycans in the matrix of healing dermal wounds in rats. J Ethnopharmacol. 1998;59(3):179-86.
[18]Jarahi M, Zahedi khorasani M, Ajorloo M, Taherian A. Local effect of Aloe barbadensis Miller gel on skin incisional wound healing in Rat. J Gorgan Univ Med Sci. 2009;11(1);13-7.
[19]Allah Tavakoli M, Vazirinejad R, Ansari Jaberi A, Negahban T, Mashayekhi H, Nazari M, et al. Effect of Teucrium polium extract on skin wound healing in rat. Med J Hormozhan Univ. 2012;16(1):17-24.
[20]Henriques A, Jackson S, Cooper R, Burton N. Free radical production and quenching in honeys with wound healing potential. J Antimicrob Chemother. 2006;58(4):773-7.
[21]Jin-Yuarn Lin, Chia-Yuan Li, I-Farn Hwang. Characterisation of the pigment components in red cabbage (Brassica oleracea L. var.) juice and their anti-inflammatory effects. Food Chem. 2008;109:771-81.
[22]Yu N, Zhai X, Xin C. An experimental studyof rabbits' wound repair by amniotic carriercomplex membrane containing bFGF andvitamin C and loaded withBMSCs. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008;22(12):1495-500. [Chinese]
[23]Hemmati AA. Effects of Scrophularia striata Extract on wound Healing in Rabbit. J Ilam Univ Med Sci. 2010;17(4);9-16.
[24]Bahorun T, Luximon-Ramma A, Crozier A, Arioma OI. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. J Sci Food Agric. 2004;84(12):1553-61.
[25]Samuelsen AB, Westereng B, Yousif O, Holtekjlen AK, Michaelsen TE, Knutsen SH. Structural features and complement-fixing activity of pectin from three Brassica oleracea varieties: white cabbage,kale and red kale. Biomacromolecules. 2007;8(2):644-9.
[26]Hasanzadeh GR, Mehdikhanloo N. The effect of compound of Brassica Oleracea L. and egg-white on burn wound healing in rat. Q J Sabzevar Univ Med Sci. 2005;11(4):6-12.
[27]Hübner G, Brauchle M, Smola H, Madlener M, Fässler R, Werner S. Differential regulation of pro-inflammatory cytokines during wound healing in normal and glucocorticoid-treated mice. Cytokine. 1996;8(7):548-56.
[28]Cartea ME, Francisco M, Soengas P, Velasco P. Phenolic compounds in Brassica vegetables. Molecules. 2011;16(1):251-80.
[2]Campos AC, Groth AK, Branco AB. Assessment and nutritional aspects of wound healing. Curr Opin Clin Nutr Metab Care. 2008;11(3):281-8.
[3]Sauermann K, Jaspers S, Koop U, Wenck H. Topically applied vitamin C increases the density of dermal papillae in aged human skin. BMC Dermatol. 2004;4:13.
[4]Tanaka T, Horiuchi G, Matsuoka M, Hirano K, Tokumura A, Koike T, et al. Formation of lysophosphatidic acid, a wound-healing lipid, during digestion of cabbage leaves. Biosci Biotechnol Biochem. 2009;73(6):1293-300.
[5]Heo HJ, Lee CY. Phenolic phytochemicals in cabbage inhibit amyloid b protein-induced neurotoxicity. LWT Food Sci Technol. 2009;39(4):331-7.
[6]Mirzai N, Vatanchian M. Effect of aqueous extract of (Olea europaea L.) on skin wound healing in mice. Q J Dev Biol. 2010;2(9):7-14.
[7]Schreml S, Szeimies RM, Prantl L, Landthaler M, Babilas P. Wound healing in the 21st century. J Am Acad Dermatol. 2010;63(5):866-81.
[8]Lin JY, Li CY, Hwang IF. Characterisation of the pigment components in red cabbage (Brassica oleracea L. var.) juice and their anti-inflammatory effects on LPS-stimulated murine splenocytes. Food Chem. 2008;109(4):771-81.
[9]Burns JL, Mancoll JS, Phillips LG. Impairments to wound healing. Clinics Plast Surg. 2003;30(1):47-56.
[10]Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M. Low-level laser therapy for wound healing, mechanism and efficacy. Dermatol Surg. 2005;31(3):334-40.
[11]Ruiz P.A, Braune A, Holzlwimmer G, Qu uintanilla-Fend L, Haller D. Quercetin inhibits TNF-induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells. J Nutr. 2007;137(5):1208-15.
[12]Jia Y, Zhao G, Jia J. Preliminary evaluation: the effects of Aloe Ferox Miller and Aleo arborescens Miller in wound healing. J Ethnopharmacol. 2008;120(2):181-9.
[13]Suguna L, Sivakumar P, Chandrakasan G. Effects of Centella asiaticaextract on dermal wound healing in rats. Indian J Exp Biol. 1996;34(12):1208-11.
[14]Paiva LA, de Alencar Cunha KM, Santos FA, Gramosa NV, Silveira ER, Rao VS. Investigation on the wound healing activity of oleoresin from copaifera langsdorffi in rats. Phytother Res. 2002;16(8):737-9.
[15]Townsend Jr CM, Beauchamp RD, Evers MD BM, Mattox MD L. Sabiston textbook of surgery: The biological basis of modern surgical practice. 19th ed. New York: Saunders; 2012.
[16]Krishnan P. The scientific study of herbal wound healing therapies: Current state of play. Curr Anaesth Crit Care. 2009;17(1-2):21-7.
[17]Chithra P, Sajithlal GB, Chandrakasan G. Influence of Aloe vera on the glycosaminoglycans in the matrix of healing dermal wounds in rats. J Ethnopharmacol. 1998;59(3):179-86.
[18]Jarahi M, Zahedi khorasani M, Ajorloo M, Taherian A. Local effect of Aloe barbadensis Miller gel on skin incisional wound healing in Rat. J Gorgan Univ Med Sci. 2009;11(1);13-7.
[19]Allah Tavakoli M, Vazirinejad R, Ansari Jaberi A, Negahban T, Mashayekhi H, Nazari M, et al. Effect of Teucrium polium extract on skin wound healing in rat. Med J Hormozhan Univ. 2012;16(1):17-24.
[20]Henriques A, Jackson S, Cooper R, Burton N. Free radical production and quenching in honeys with wound healing potential. J Antimicrob Chemother. 2006;58(4):773-7.
[21]Jin-Yuarn Lin, Chia-Yuan Li, I-Farn Hwang. Characterisation of the pigment components in red cabbage (Brassica oleracea L. var.) juice and their anti-inflammatory effects. Food Chem. 2008;109:771-81.
[22]Yu N, Zhai X, Xin C. An experimental studyof rabbits' wound repair by amniotic carriercomplex membrane containing bFGF andvitamin C and loaded withBMSCs. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008;22(12):1495-500. [Chinese]
[23]Hemmati AA. Effects of Scrophularia striata Extract on wound Healing in Rabbit. J Ilam Univ Med Sci. 2010;17(4);9-16.
[24]Bahorun T, Luximon-Ramma A, Crozier A, Arioma OI. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. J Sci Food Agric. 2004;84(12):1553-61.
[25]Samuelsen AB, Westereng B, Yousif O, Holtekjlen AK, Michaelsen TE, Knutsen SH. Structural features and complement-fixing activity of pectin from three Brassica oleracea varieties: white cabbage,kale and red kale. Biomacromolecules. 2007;8(2):644-9.
[26]Hasanzadeh GR, Mehdikhanloo N. The effect of compound of Brassica Oleracea L. and egg-white on burn wound healing in rat. Q J Sabzevar Univ Med Sci. 2005;11(4):6-12.
[27]Hübner G, Brauchle M, Smola H, Madlener M, Fässler R, Werner S. Differential regulation of pro-inflammatory cytokines during wound healing in normal and glucocorticoid-treated mice. Cytokine. 1996;8(7):548-56.
[28]Cartea ME, Francisco M, Soengas P, Velasco P. Phenolic compounds in Brassica vegetables. Molecules. 2011;16(1):251-80.