@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(2):91-96
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(2):91-96
Modulate the Effects of the Bone Marrow–Derived Mesenchymal Stem Cells’ Supernatant on Neutrophil Functions by 17-beta Estradiol
ARTICLE INFO
Article Type
Original ResearchAuthors
Afzal Ahangran N. (*)Delirejh N. (1)
Nekoeii Z. (1)
(*) Microbiology Department, Veterinary Faculty, Urmia University, Urmia, Iran
(1) Microbiology Department, Veterinary Faculty, Urmia University, Urmia, Iran
Correspondence
Address: Microbiology Department, Veterinary Faculty, Nazlu Pardis, Kilometer 11 of Sarv Road, Urmia, IranPhone: +984432770508
Fax: +984432771926
n.a.ahangran@gmail.com
Article History
Received: November 25, 2014Accepted: May 10, 2015
ePublished: June 20, 2015
ABSTRACT
Aims
Bone marrow-derived mesenchymal stem cells have therapeutic potentials due to their immunomodulatory properties. Estrogen is also an immunomodulator. The current study was to analyze the effect of bone marrow-derived mesenchymal stem cells’ supernatant of male rats adjacent with estrogen on the function and survival of neutrophils.
Materials & Methods In this experimental study, mesenchymal stem cells isolated from the femur and tibia of the bone marrow of male 6-8 week old rats were cultured in DMEM. After maturation, the supernatant of the mesenchymal stem cells treated with estrogen (10nM and 20nM) and cultured for 72 hours at 37°C. Then, the mesenchymal stem cells were co-cultured with the peripheral blood neutrophils and the neutrophil functions were measured by phagocytosis and respiratory burst (Nitro Blue Tetrazolium resuscitation) tests. The viability of neutrophils was measured with acridine-orange fluorescent staining. Data were analyzed by SPSS 18 software, using independent T, one way ANOVA and Tukey tests.
Findings The respiratory burst in the groups treated with 10nM and 20nM of estrogen showed significant difference compared with the control group. The percentage of phagocytosis in the groups treated with 10nM and 20nM of estrogen were significantly increased compared with the control group. There was a significant difference between the percentage of phagocytosis of 10nM and 20nm groups (p<0.05). The apoptosis level in the groups treated with 10nM and 20nM of estrogen showed significant difference compared with the control group.
Conclusion Supernatant of mesenchymal stem cells treated with estrogen increases the phagocytosis potential and respiratory explosion of neutrophils.
Materials & Methods In this experimental study, mesenchymal stem cells isolated from the femur and tibia of the bone marrow of male 6-8 week old rats were cultured in DMEM. After maturation, the supernatant of the mesenchymal stem cells treated with estrogen (10nM and 20nM) and cultured for 72 hours at 37°C. Then, the mesenchymal stem cells were co-cultured with the peripheral blood neutrophils and the neutrophil functions were measured by phagocytosis and respiratory burst (Nitro Blue Tetrazolium resuscitation) tests. The viability of neutrophils was measured with acridine-orange fluorescent staining. Data were analyzed by SPSS 18 software, using independent T, one way ANOVA and Tukey tests.
Findings The respiratory burst in the groups treated with 10nM and 20nM of estrogen showed significant difference compared with the control group. The percentage of phagocytosis in the groups treated with 10nM and 20nM of estrogen were significantly increased compared with the control group. There was a significant difference between the percentage of phagocytosis of 10nM and 20nm groups (p<0.05). The apoptosis level in the groups treated with 10nM and 20nM of estrogen showed significant difference compared with the control group.
Conclusion Supernatant of mesenchymal stem cells treated with estrogen increases the phagocytosis potential and respiratory explosion of neutrophils.
CITATION LINKS
[1]Yin T, Li L. The stem cell niches in bone. J Clin Invest. 2006;116(5):1195-201.
[2]Beresford JN. Osteogenic stem cells and the stromal system of bone and marrow. Clin Orthop Relat Res. 1989;(240):270-80.
[3]Raffaghello L, Bianchi G, Bertolotto M, Montecucco F, Busca A, Dallegri F, et al. Human mesenchymal stem cells inhibit neutrophil apoptosis: Α model for neutrophil preservation in the bone marrow niche. Stem Cells. 2008;26(1):151-62.
[4]O'Garra A, Barrat FJ, Castro AG, Vicari A, Hawrylowicz C. Strategies for use of IL-10 or its antagonists in human disease. Immunol Rev. 2008;223:114-31.
[5]Xu G, Zhang Y, Zhang L, Ren G, Shi Y. The role of IL-6 in inhibition of lymphocyte apoptosis by mesenchymal stem cells. Biochem Biophys Res Commun. 2007;361(3):745-50.
[6]Benvenuto F, Ferrari S, Gerdoni E, Gualandi F, Frassoni F, Pistoia V, et al. Human mesenchymal stem cells promote survival of T cells in a quiescent state. Stem Cells. 2007;25(7):1753-60.
[7]Gaillard RC, Spinedi E. Sex-and stress-steroids interactions and the immune system: Evidence for a neuroendocrine-immunological sexual dimorphism. Domest Anim Endocrinol. 1998;15(5):345-52.
[8]Hashemi M, Kroczak TJ. Apoptosis in autoimmune diseases. Curr Med Chem. 2005;4(4):429-37.
[9]Bittencourt RAC, Pereira HR, Felisbino SL, Murador P, Oliveira APE, Deffune E. Isolation of Bone marrow mesenchymal stem cells. Acta Ortop Bras. 2006;14(1):22-4.
[10]Rezapour A, Majidi J. An improved method of neutrophil isolation in peripheral blood of sheep. J Anim Vet Adv. 2009;8(1):11-5.
[11]Hashemi M, Ghavami S. Effects of estradiol, progesterone and testosterone on proliferation of human breast cancer cell lines. Tabib-E-Shargh. 2005;7(1):21-9. [Persian]
[12]Esmaili Gouvarchin Galeh H, Delirezh N. Calcitriol modulates the effects of the supernatants of bone-marrow-derived mesenchymal stem cells on neutrophil functions. Turk J Biol. 2014;38(3):365-70.
[13]Turina M, Miller FN, Mc Hugh PP, Cheadle WG, Polk HC. Endotoxin inhibits apoptosis but induces primary necrosis in neutrophils. Inflammation. 2005;29(1):55-63.
[14]Ghoseiri R, Alizadeh S, Mojtahedzadeh F, Najafi Poor H. Survey of the effect of powder nigella sativa (black seed) in increscent of monocyte phagocytosis in quinea pig. Horizon Med Sci. 2010;16(3):55-64. [Persian]
[15]Hamaliaka A, Novikova I. Nitric oxide production disorders in leukocytes of patients with recurrent furunculosis. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2010;154(2):367-72.
[16]Glennie S, Soeiro I, Dyson PJ, Lam EW, Dazzi F. Bone marrow mesenchymal stem cells induce division arrest anergy of activated T cells. Blood. 2005;105(7):2821-7.
[17]Corcione A1, Benvenuto F, Ferretti E, Giunti D, Cappiello V, Cazzanti F, et al. Human mesenchymal stem cells modulate B-cell functions. Blood. 2006;107(1):367-72.
[18]Spaggiari GM, Capobianco A, Becchetti S, Mingari MC, Moretta L. Mesenchymal stem cell-natural killer cell interactions: evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL-2-induced NK-cell proliferation. Blood. 2006;107(4):1484-90.
[19]Brandau S, Jakob M, Hemeda H, Bruderek K, Janeschik S, Bootz F, et al. Tissue-resident mesenchymal stem cells attract peripheral blood neutrophils and enhance their inflammatory activity in response to microbial challenge. J Leukoc Biol. 2010;88(5):1005-15.
[20]Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood. 2005;105(4):1815-22.
[21]Beyth S, Borovsky Z, Mevorach D, Liebergall M, Gazit Z, Aslan H, et al. Human mesenchymal stem cells alter antigen-presenting cell maturation and induce T-cell unresponsiveness. Blood. 2005;105(5):2214-9.
[22]Ghannam S, Bouffi C, Djouad F, Jorgensen C, Noël D. Immunosuppression by mesenchymal stem cells: mechanisms and clinical applications. Stem Cell Res Ther. 2010;1(1):2.
[23]Maby El Hajjami H, Amé Thomas P, Pangault C, Tribut O, De Vos J, Jean R, et al. Functional alteration of the lymphoma stromal cell niche by the cytokine context: Role of indoleamine-2,3 dioxygenase. Cancer Res. 2009;69(7):3228-37.
[24]Ansar Ahmed S, Dauphinée MJ, Montoya AI, Talal N. Estrogen induces normal murine CD5+B cells to produce autoantibodies. J Immunol. 1989;142(8):2647-53.
[25]Schilling T, Ebert R, Raaijmakers N, Schütze N, Jakob F. Effects of phytoestrogens and other plant-derived compounds on mesenchymal stem cells, bone maintenance and regeneration. J Steroid Biochem Mol Biol. 2014;139:252-61.
[26]Blesson CB. Estrogen receptors in leukocytes- possible impact on inflammatory processes in the female reproductive system. In: Aimaretti G, Marzullo P, Prodam F, (editors). Endocrinology and metabolism update on mechanisms of hormone action: Focus on metabolism, growth and reproduction. Rijeka, Croatia: InTECH; 2011.
[27]Krasnodembskaya A, Song Y, Fang X, Gupta N, Serikov V, Lee JW. Antibacterial effect of human mesenchymal stem cells is mediated in part from secretion of the antimicrobial peptide LL-37. Stem Cells. 2010;28(12):2229-38.
[2]Beresford JN. Osteogenic stem cells and the stromal system of bone and marrow. Clin Orthop Relat Res. 1989;(240):270-80.
[3]Raffaghello L, Bianchi G, Bertolotto M, Montecucco F, Busca A, Dallegri F, et al. Human mesenchymal stem cells inhibit neutrophil apoptosis: Α model for neutrophil preservation in the bone marrow niche. Stem Cells. 2008;26(1):151-62.
[4]O'Garra A, Barrat FJ, Castro AG, Vicari A, Hawrylowicz C. Strategies for use of IL-10 or its antagonists in human disease. Immunol Rev. 2008;223:114-31.
[5]Xu G, Zhang Y, Zhang L, Ren G, Shi Y. The role of IL-6 in inhibition of lymphocyte apoptosis by mesenchymal stem cells. Biochem Biophys Res Commun. 2007;361(3):745-50.
[6]Benvenuto F, Ferrari S, Gerdoni E, Gualandi F, Frassoni F, Pistoia V, et al. Human mesenchymal stem cells promote survival of T cells in a quiescent state. Stem Cells. 2007;25(7):1753-60.
[7]Gaillard RC, Spinedi E. Sex-and stress-steroids interactions and the immune system: Evidence for a neuroendocrine-immunological sexual dimorphism. Domest Anim Endocrinol. 1998;15(5):345-52.
[8]Hashemi M, Kroczak TJ. Apoptosis in autoimmune diseases. Curr Med Chem. 2005;4(4):429-37.
[9]Bittencourt RAC, Pereira HR, Felisbino SL, Murador P, Oliveira APE, Deffune E. Isolation of Bone marrow mesenchymal stem cells. Acta Ortop Bras. 2006;14(1):22-4.
[10]Rezapour A, Majidi J. An improved method of neutrophil isolation in peripheral blood of sheep. J Anim Vet Adv. 2009;8(1):11-5.
[11]Hashemi M, Ghavami S. Effects of estradiol, progesterone and testosterone on proliferation of human breast cancer cell lines. Tabib-E-Shargh. 2005;7(1):21-9. [Persian]
[12]Esmaili Gouvarchin Galeh H, Delirezh N. Calcitriol modulates the effects of the supernatants of bone-marrow-derived mesenchymal stem cells on neutrophil functions. Turk J Biol. 2014;38(3):365-70.
[13]Turina M, Miller FN, Mc Hugh PP, Cheadle WG, Polk HC. Endotoxin inhibits apoptosis but induces primary necrosis in neutrophils. Inflammation. 2005;29(1):55-63.
[14]Ghoseiri R, Alizadeh S, Mojtahedzadeh F, Najafi Poor H. Survey of the effect of powder nigella sativa (black seed) in increscent of monocyte phagocytosis in quinea pig. Horizon Med Sci. 2010;16(3):55-64. [Persian]
[15]Hamaliaka A, Novikova I. Nitric oxide production disorders in leukocytes of patients with recurrent furunculosis. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2010;154(2):367-72.
[16]Glennie S, Soeiro I, Dyson PJ, Lam EW, Dazzi F. Bone marrow mesenchymal stem cells induce division arrest anergy of activated T cells. Blood. 2005;105(7):2821-7.
[17]Corcione A1, Benvenuto F, Ferretti E, Giunti D, Cappiello V, Cazzanti F, et al. Human mesenchymal stem cells modulate B-cell functions. Blood. 2006;107(1):367-72.
[18]Spaggiari GM, Capobianco A, Becchetti S, Mingari MC, Moretta L. Mesenchymal stem cell-natural killer cell interactions: evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL-2-induced NK-cell proliferation. Blood. 2006;107(4):1484-90.
[19]Brandau S, Jakob M, Hemeda H, Bruderek K, Janeschik S, Bootz F, et al. Tissue-resident mesenchymal stem cells attract peripheral blood neutrophils and enhance their inflammatory activity in response to microbial challenge. J Leukoc Biol. 2010;88(5):1005-15.
[20]Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood. 2005;105(4):1815-22.
[21]Beyth S, Borovsky Z, Mevorach D, Liebergall M, Gazit Z, Aslan H, et al. Human mesenchymal stem cells alter antigen-presenting cell maturation and induce T-cell unresponsiveness. Blood. 2005;105(5):2214-9.
[22]Ghannam S, Bouffi C, Djouad F, Jorgensen C, Noël D. Immunosuppression by mesenchymal stem cells: mechanisms and clinical applications. Stem Cell Res Ther. 2010;1(1):2.
[23]Maby El Hajjami H, Amé Thomas P, Pangault C, Tribut O, De Vos J, Jean R, et al. Functional alteration of the lymphoma stromal cell niche by the cytokine context: Role of indoleamine-2,3 dioxygenase. Cancer Res. 2009;69(7):3228-37.
[24]Ansar Ahmed S, Dauphinée MJ, Montoya AI, Talal N. Estrogen induces normal murine CD5+B cells to produce autoantibodies. J Immunol. 1989;142(8):2647-53.
[25]Schilling T, Ebert R, Raaijmakers N, Schütze N, Jakob F. Effects of phytoestrogens and other plant-derived compounds on mesenchymal stem cells, bone maintenance and regeneration. J Steroid Biochem Mol Biol. 2014;139:252-61.
[26]Blesson CB. Estrogen receptors in leukocytes- possible impact on inflammatory processes in the female reproductive system. In: Aimaretti G, Marzullo P, Prodam F, (editors). Endocrinology and metabolism update on mechanisms of hormone action: Focus on metabolism, growth and reproduction. Rijeka, Croatia: InTECH; 2011.
[27]Krasnodembskaya A, Song Y, Fang X, Gupta N, Serikov V, Lee JW. Antibacterial effect of human mesenchymal stem cells is mediated in part from secretion of the antimicrobial peptide LL-37. Stem Cells. 2010;28(12):2229-38.