ARTICLE INFO

Article Type

Original Research

Authors

Kermany   H. (1)
Shahanipour   K. (*)
Nakhaee   A.R. (2)






(*) Biochemistry Department, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
(1) Biochemistry Department, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
(2) Biochemistry Department, Medicine Faculty, Zahedan University of Medical Sciences, Zahedan, Iran

Correspondence

Address: Department of Biochemistry, Islamic Azad University, Daneshgah Boulevard, Basij Boulevard, Falavarjan, Iran
Phone: +983117725585
Fax: +983133390000
shahanipur_k@yahoo.com

Article History

Received:   November  30, 2014
Accepted:   May 10, 2015
ePublished:   June 20, 2015

ABSTRACT

Aims Karela (Momordica charantia), is extensively used in traditional medicine as an antidiabetic drug. The aim of the present study was to analyze the effect of aqueous and methanolic extrarct of Momordica charantia on blood glucose and liver enzymes of rats.
Materials & Methods In this experimental study, 36 adult male Wistar rats were divided into 6 groups (6 rats in each group); control (healthy rats), negative control (diabetic rats by Streptozotocine) and 4 experimental groups (diabetic rats which recived aqueous and methanolic extracts of plant in concentrations of 50 and 100mg/kg of body weight daily for 40 days). The amount of the blood glucose was determined by sequential incision of the tip of the tail once a weak. After completing 40 days, blood was collected from heart of rats and the level of the liver enzymes serum was determined. The results were analysed by ANOVA with repeated measure and one way ANOVA tests in SPSS 19 software.
Findings The effect of different concentrations of aqueous and methanolic extracts on the blood glucose level was significant (p<0.001). Also a significant difference was observed between the amount of the aspartate aminotransfrase (p=0.049) and alanin aminotransfrase (p=0.005) enzymes activity at the two groups of negative control and 50mg/kg of body weight of methanolic extract. Using of 50 and 100mg/kg of body weight of aqueous and methanolic extracts reduced the alkalin phosphatase enzyme activity (p=0.002).
Conclusion Aqueous extract of Momordica charantia fruit have better effect on reducing the blood glucose than methanolic extracts. Both aqueous and methanolic extracts reduce the activity of aspartate aminotransfrase, alanin aminotransfrase and alkalin phosphatase.

Keywords: Momordica charantia , Diabetes Mellitus , Liver, Rats,

CITATION LINKS

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