@2024 Afarand., IRAN
ISSN: 2008-2630 Iranian Journal of War & Public Health 2014;6(5):183-187
ISSN: 2008-2630 Iranian Journal of War & Public Health 2014;6(5):183-187
Correlation of Oxidative Stress Indices with Age in Chemical Veterans with Severe Pulmonary Complication; Longtime after Exposition to Sulfur Mustard
ARTICLE INFO
Article Type
Original ResearchAuthors
Soroush M.R. (1)Faday Vatan R (*)
Sahaf R (2)
Taravati A (3)
Ghazanfari T (4)
Faghihzadeh S (5)
Kaboudiyan Ardestani S (6)
(*) Iranian Research Center on Aging, Social Welfare and Rehabilitation Sciences University, Tehran, Iran
(1) Janbazan Medical and Engineering Research Center, Tehran, Iran
(2) 2Iranian Research Center on Aging, Social Welfare and Rehabilitation Sciences University, Tehran, Iran
(3) Cell and Molecular Biology Department, Basic Sciences Faculty, University of Mazandaran, Babolsar, Iran
(4) Immunoregulation Research Center, Shahed University, Tehran, Iran
(5) Biostatistics Department, Statistics Faculty, Zanjan University of Medical Sciences, Zanjan, Iran
(6) Institute of Biochemistry and Biophysics, Tehran University, Tehran, Iran
Correspondence
Article History
Received: March 21, 2014Accepted: May 26, 2014
ePublished: November 6, 2014
ABSTRACT
Aims
Aging is the result of accumulation of different detrimental changes in cells and tissues
that increase the risk of disease and death with aging. Irreversible and progressive oxidative
hindrance caused by “reactive oxygen species” is effective in aging. This study examined the
association of age with oxidative stress indices in patients with severe pulmonary lesions by
measuring the levels of these indices, 15-20 years after exposure.
Materials & Methods This cross-sectional study was done on 289 veterans with severe pulmonary lesions and 66 identical normal subjects in 2008. Blood samples were taken in the fasting state. ELISA method was used for measuring carbonyl protein and serum total antioxidant, Thiobarbituric Acid reaction for lipid peroxidation and FOX2 for total peroxide concentration of plasma samples. Differences between groups were measured by Student T test using SPSS 18 software.
Findings No significant differences were observed in levels of Carbonyl protein and oxidative stress index (the ratio of total antioxidant to total peroxide) between two patient and control groups, while total peroxide level of patient group was significantly (p<0.001) less than control group. No significant correlation was seen between age and oxidative stress indices in both groups (p>0.05).
Conclusion In order to eliminate and control the response to mustard gas in bodies of chemical injured people, total antioxidant increases and total peroxide decreases and oxidative stress index remains unchanged.
Materials & Methods This cross-sectional study was done on 289 veterans with severe pulmonary lesions and 66 identical normal subjects in 2008. Blood samples were taken in the fasting state. ELISA method was used for measuring carbonyl protein and serum total antioxidant, Thiobarbituric Acid reaction for lipid peroxidation and FOX2 for total peroxide concentration of plasma samples. Differences between groups were measured by Student T test using SPSS 18 software.
Findings No significant differences were observed in levels of Carbonyl protein and oxidative stress index (the ratio of total antioxidant to total peroxide) between two patient and control groups, while total peroxide level of patient group was significantly (p<0.001) less than control group. No significant correlation was seen between age and oxidative stress indices in both groups (p>0.05).
Conclusion In order to eliminate and control the response to mustard gas in bodies of chemical injured people, total antioxidant increases and total peroxide decreases and oxidative stress index remains unchanged.
CITATION LINKS
[1]Khateri S, Ghanei M, Keshavarz S, Soroush M, Haines D. Incidence of lung, eye, and skin lesions as late complications in 34,000 Iranians with wartime exposure to mustard agent. J Occup Environ Med. 2003;45(11):1136-43
[2]Sidell FR, Takafuji ET, Franz DR. Medical Aspects of Chemical and Biological Warfare (Textbook of Military Medicine. Part 1; Warfare, Weaponry, and the Casualty, Volume 3). 1st ed. Washington: United States Government Printing; 1997.
[3]U.N. Security Council. Report of the specialists appointed by the secretory general to investigate allegations by Iran concerning the use of chemical weapons [Internet]. New York: Wisconsin Project on Nuclear Arms Control. [Cited: March 26, 1984]. Available from: http://www.iranwatch.org/library/international-organization/united-nations-un/un-security-council/report-specialist-appointed-secretary-general-investigate-allegations-islamic.
[4]Kumar O, Sugendran K, Vijayaraghavan R. Protective effect of various antioxidants on the toxicity of sulphur mustard administered to mice by inhalation or percutaneous routes. Chem Biol Interact. 2001;134(1):1-12.
[5]Andziak B, O'Connor TP, Qi W, DeWaal EM, Pierce A, Chaudhuri AR, et al. High oxidative damage levels in the longest-living rodent, the naked mole-rat. Aging Cell. 2006;5(6):463-71.
[6]Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8.
[7]Balali-Mood M, Hefazi M. Comparison of early and late toxic effects of sulfur mustard in Iranian veterans. Basic Clin Pharmacol Toxicol. 2006;99(4):273-82.
[8]Miyazawa T. Determination of phospholipid hydroperoxides in human blood plasma by a chemiluminescence-HPLC assay. Free Radic Biol Med. 1989;7(2):209-17.
[9]Zarchi K, Akbar A, Naieni KH. Long term pulmonary complications in compatants exposed to mustard gas: A historical cohort study. Int J Epidemiol. 2004;33(3):579-81.
[10]Shohrati M, Ghanei M, Shamspour N, Jafari M. Activity and function in lung injuries due to sulphur mustard. Biomarkers. 2008;13(7):728-33.
[11]Taravati A, Ardestani SK, Ziaee AA, Ghorbani A, Soroush MR, Faghihzadeh S, et al. Effects of paraoxonase 1 activity and gene polymorphisms on long-term pulmonary complications of sulfur mustard-exposed veterans. Int Immunopharmacol. 2013;17(3):974-9.
[12]Kapahi P, Boulton ME, Kirkwood TB. Positive correlation between mammalian life span and cellular resistance to stress. Free Radic Biol Med. 1999;26(5-6):495-500.
[13]Ashok BT, Ali R. Binding of circulating antibodies to reactive oxygen species modified-DNA and detecting DNA damage by a monoclonal antibody probe. Mech Ageing Dev. 1998;103(1):69-80.
[14]Stadtman ER, Starke-Reed PE, Oliver CN, Carney JM, Floyd RA. Protein modification in aging. In: Emerit I, Chance B. Free Radicals and Aging (Volume 62). Switzerland: Birkhäuser Basel; 1992. pp. 64-72.
[15]Landis GN, Tower J. Superoxide dismutase evolution and life span regulation. Mech Ageing Dev. 2005;126(3):365-79.
[16]Jafari M. Dose- and time-dependent effects of sulfur mustard on antioxidant system in liver and brain of rat. Toxicology. 2007;231(1):30-9.
[2]Sidell FR, Takafuji ET, Franz DR. Medical Aspects of Chemical and Biological Warfare (Textbook of Military Medicine. Part 1; Warfare, Weaponry, and the Casualty, Volume 3). 1st ed. Washington: United States Government Printing; 1997.
[3]U.N. Security Council. Report of the specialists appointed by the secretory general to investigate allegations by Iran concerning the use of chemical weapons [Internet]. New York: Wisconsin Project on Nuclear Arms Control. [Cited: March 26, 1984]. Available from: http://www.iranwatch.org/library/international-organization/united-nations-un/un-security-council/report-specialist-appointed-secretary-general-investigate-allegations-islamic.
[4]Kumar O, Sugendran K, Vijayaraghavan R. Protective effect of various antioxidants on the toxicity of sulphur mustard administered to mice by inhalation or percutaneous routes. Chem Biol Interact. 2001;134(1):1-12.
[5]Andziak B, O'Connor TP, Qi W, DeWaal EM, Pierce A, Chaudhuri AR, et al. High oxidative damage levels in the longest-living rodent, the naked mole-rat. Aging Cell. 2006;5(6):463-71.
[6]Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8.
[7]Balali-Mood M, Hefazi M. Comparison of early and late toxic effects of sulfur mustard in Iranian veterans. Basic Clin Pharmacol Toxicol. 2006;99(4):273-82.
[8]Miyazawa T. Determination of phospholipid hydroperoxides in human blood plasma by a chemiluminescence-HPLC assay. Free Radic Biol Med. 1989;7(2):209-17.
[9]Zarchi K, Akbar A, Naieni KH. Long term pulmonary complications in compatants exposed to mustard gas: A historical cohort study. Int J Epidemiol. 2004;33(3):579-81.
[10]Shohrati M, Ghanei M, Shamspour N, Jafari M. Activity and function in lung injuries due to sulphur mustard. Biomarkers. 2008;13(7):728-33.
[11]Taravati A, Ardestani SK, Ziaee AA, Ghorbani A, Soroush MR, Faghihzadeh S, et al. Effects of paraoxonase 1 activity and gene polymorphisms on long-term pulmonary complications of sulfur mustard-exposed veterans. Int Immunopharmacol. 2013;17(3):974-9.
[12]Kapahi P, Boulton ME, Kirkwood TB. Positive correlation between mammalian life span and cellular resistance to stress. Free Radic Biol Med. 1999;26(5-6):495-500.
[13]Ashok BT, Ali R. Binding of circulating antibodies to reactive oxygen species modified-DNA and detecting DNA damage by a monoclonal antibody probe. Mech Ageing Dev. 1998;103(1):69-80.
[14]Stadtman ER, Starke-Reed PE, Oliver CN, Carney JM, Floyd RA. Protein modification in aging. In: Emerit I, Chance B. Free Radicals and Aging (Volume 62). Switzerland: Birkhäuser Basel; 1992. pp. 64-72.
[15]Landis GN, Tower J. Superoxide dismutase evolution and life span regulation. Mech Ageing Dev. 2005;126(3):365-79.
[16]Jafari M. Dose- and time-dependent effects of sulfur mustard on antioxidant system in liver and brain of rat. Toxicology. 2007;231(1):30-9.