@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;20(4):243-248
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;20(4):243-248
Comparison the Effect of Hydroalcholic Extract of Nigella Sativa L. Seed and Metformin on Blood Biochemical Parameters in Streptozotocin-Induced Diabetic Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Abbasnezhad A.A. (1)Niyazmand S. (*)
Mahmoud Abadi M. (1)
Soukhtanloo M. (2)
Rezaee S.A. (3 )
Mousavi S.M. (1)
(*) Physiology Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(1) Physiology Department , Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(1) Physiology Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(1) Physiology Department , Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(2) Biochemistry Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(3 ) Immunology Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
Correspondence
Address: Physiology Department, Medicine Faculty, Mashhad University of Medical Sciences, Azadi Square, Mashhad, IranPhone: +985138002225
Fax: +985138828564
niazmands@mums.ac.ir
Article History
Received: October 19, 2014Accepted: December 29, 2014
ePublished: February 19, 2015
ABSTRACT
Aims
Diabetes mellitus is associated by increased blood glucose level resulting from lack of Insulin or Insulin resistance in peripheral tissues or both. The aim of this study was to investigate the effects of Nigella sativa L. seed hydroalcoholic extract on blood biochemical parameters in STZ-induced diabetic rats.
Materials & Methods This interventional study was done on 60 male Wistar rats. Animals were randomly divided into 6 groups (n=10) including control, diabetic (receiving saline), groups receiving 100, 200 and 400mg/kg doses of the extract and positive control (receiving 300mg/kg Metformin). Animals were treated for 6 weeks with gavage from start (zero), 24 and 45 days of the study. Glucose, cholesterol, triglycerides, HDL and LDL were measured after 12hours of fasting. Data were analyzed by SPSS 16 software and One-way ANOVA, Tukey and Paired-T tests.
Findings Blood sugar levels in diabetic rats receiving 200mg/kg dose of the extract was reduced more significantly than other treated groups (p<0.01). There was a significant difference in cholesterol and triglyceride at day 45 in all treated groups compared to diabetic group (p<0.001). No significant difference was observed in HDL in groups. A significant reduction in LDL was observed in diabetic group treated with Metformin and Nigella extract at day 45.
Conclusion Nigella sativa L. extract reduces glucose, cholesterol, triglyceride, and LDL levels in diabetic rats.
Materials & Methods This interventional study was done on 60 male Wistar rats. Animals were randomly divided into 6 groups (n=10) including control, diabetic (receiving saline), groups receiving 100, 200 and 400mg/kg doses of the extract and positive control (receiving 300mg/kg Metformin). Animals were treated for 6 weeks with gavage from start (zero), 24 and 45 days of the study. Glucose, cholesterol, triglycerides, HDL and LDL were measured after 12hours of fasting. Data were analyzed by SPSS 16 software and One-way ANOVA, Tukey and Paired-T tests.
Findings Blood sugar levels in diabetic rats receiving 200mg/kg dose of the extract was reduced more significantly than other treated groups (p<0.01). There was a significant difference in cholesterol and triglyceride at day 45 in all treated groups compared to diabetic group (p<0.001). No significant difference was observed in HDL in groups. A significant reduction in LDL was observed in diabetic group treated with Metformin and Nigella extract at day 45.
Conclusion Nigella sativa L. extract reduces glucose, cholesterol, triglyceride, and LDL levels in diabetic rats.
CITATION LINKS
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[2]Azimi-Nezhad M, Ghayour-Mobarhan M, Parizadeh MR, Safarian M, Esmaeili H, Parizadeh SM, et al. Prevalence of type 2 diabetes mellitus in Iran and its relationship with gender, urbanisation, education, marital status and occupation. Singapore Med J. 2008;49(7):571-6.
[3]Momin M, Momin S, Kurhade S, Butte K. Niglla sativa: Blessed seed. Res Phyochem Pharmachol. 2013;3(2):78-84.
[4]Deshpande AD, Harris-Hayes M, Schootman M. Epidemiology of diabetes and diabetes-related complications. Phys Therapy. 2008;88(11):1254-64.
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[7]Dattner AM. From medical herbalism to phytotherapy in dermatology: back to the future. Dermatol Ther. 2003;16(2):106-13.
[8]Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed. 2013;3(5):337-52.
[9]Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res. 2003;17(4):299-305.
[10]Frode TS, Medeiros YS. Animal models to test drugs with potential antidiabetic activity. J Ethnopharmacol. 2008;115(2):173-83.
[11]Bell RH Jr, Hye RJ. Animal models of diabetes mellitus: Physiology and pathology. J Surg Res. 1983;35(5):433-60.
[12]Houcher Z, Boudiaf K, Benboubetra M, Houcher B. Effects of methanolic extract and commercial oil of Nigella sativa L. on blood glucose and antioxidant capacity in alloxan-induced diabetic rats. Pteridines. 2007;18(1):8-18.
[13]Hawsawi ZA, Ali BA, Bamosa AO. Effect of Nigella sativa (black seed) and thymoquinone on blood glucose in albino rats. Ann Saudi Med. 2001;21(3-4):242-4.
[14]Bhat M, Zinjarde SS, Bhargava SY, Kumar AR, Joshi BN. Antidiabetic Indian plants: A good source of potent amylase inhibitors Evid Based Complement Alternat Med. 2011;2011:810207.
[15]Luo JZ, Luo L. Ginseng on hyperglycemia: effects and mechanisms. Evid Based Complement Alternat Med. 2009;6(4):423-7.
[16]Samane S, Noel J, Charrouf Z, Amarouch H, Haddad PS. Insulin-sensitizing and anti-proliferative effects of Argania spinosa seed extracts. Evid Based Complement Alternat Med. 2006;3(3):317-27.
[17]Meddah B, Ducroc R, El Abbes Faouzi M, Eto B, Mahraoui L, Benhaddou-Andaloussi A, et al. Nigella sativa inhibits intestinal glucose absorption and improves glucose tolerance in rats. J Ethnopharmacol. 2009;121(3):419-24.
[18]Fararh K, Atoji Y, Shimizu Y, Shiina T, Nikami H, Takewaki T. Mechanisms of the hypoglycaemic and immunopotentiating effects of Nigella sativa L. oil in streptozotocin-induced diabetic hamsters. Res Vet Sci. 2004;77(2):123-9.
[19]Benhaddou-Andaloussi A, Martineau L, Vuong T, Meddah B, Madiraju P, Settaf A, et al. The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content. Evid Based Complement Alternat Med. 2011;2011:538671
[20]Al-Logmani A, Zari T. Long-term effects of Nigella sativa L. oil on some physiological parameters in normal and streptozotocin-induced diabetic rats. J Diabetes Mellitus. 2011;1(3):46-53.
[21]Saleh DO, Bayoumi AR, El-Eraky WI, El-Khatib AS. Streptozotocin-induced vascular and biochemical changes in rats: Effects of rosiglitazone vs. metformin. Bull Faculty Pharm Cairo Univ. 2013;51(2):131-8.
[22]Beisswenger P, Ruggiero-Lopez D. Metformin inhibition of glycation processes. 2003;29(4 Pt 2):6S95-103
[23]Klip A, Leiter LA. Cellular mechanism of action of metformin. Diabetes Care. 1990;13(6):696-704.
[24]Zhou G1, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, Wu M, et al. Role of AMP-activated protein kinase in mechanism of metformin action. J Clin Invest. 2001;108(8):1167-74.
[25]Kannel WB. Lipids, diabetes, and coronary heart disease: Insights from the Framingham Study. Am Heart J. 1985;110(5):1100-7.
[26]Kaleem M, Kirmani D, Asif M, Ahmed Q, Bano B. Biochemical effects of Nigella sativa L seeds in diabetic rats. Indian J Exp Biol. 2006;44(9):745-8.
[27]Al-Naqeep G, Al-Zubairi AS, Ismail M, Amom ZH, Esa NM. Antiatherogenic potential of Nigella sativa seeds and oil in diet-induced hypercholesterolemia in rabbits. Evid Based Complement Alternat Med. 2011;2011:213628.
[28]Hadjzadeh M-AL-R , Pilavarian AA, Hydari AA, Behnam Rassouli F. The effect of aqueous extract of Niglla sativa L. Seeds in streptozocin induced diabetic rat. Pharmacologyonline. 2008;3:986-91.
[29]Najmi A, Nasiruddin M, Khan RA, Haque SF. Effect of Nigella sativa oil on various clinical and biochemical parameters of insulin resistance syndrome. Int J Diabetes Dev Ctries. 2008;28(1):11-4.
[30]Pentikainen PJ, Voutilainen E, Aro A, Uusitupa M, Penttila I, Vapaatalo H. Cholesterol lowering effect of metformin in combined hyperlipidemia: placebo controlled double blind trial. Ann Med. 1990;22(5):307-12.
[2]Azimi-Nezhad M, Ghayour-Mobarhan M, Parizadeh MR, Safarian M, Esmaeili H, Parizadeh SM, et al. Prevalence of type 2 diabetes mellitus in Iran and its relationship with gender, urbanisation, education, marital status and occupation. Singapore Med J. 2008;49(7):571-6.
[3]Momin M, Momin S, Kurhade S, Butte K. Niglla sativa: Blessed seed. Res Phyochem Pharmachol. 2013;3(2):78-84.
[4]Deshpande AD, Harris-Hayes M, Schootman M. Epidemiology of diabetes and diabetes-related complications. Phys Therapy. 2008;88(11):1254-64.
[5]Yan SH, Sheu WH, Song YM, Tseng LN. The occurrence of diabetic ketoacidosis in adults. Am J Med. 1996;101(1):19-24.
[6]American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2010;33(Suppl 1):62-9.
[7]Dattner AM. From medical herbalism to phytotherapy in dermatology: back to the future. Dermatol Ther. 2003;16(2):106-13.
[8]Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed. 2013;3(5):337-52.
[9]Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res. 2003;17(4):299-305.
[10]Frode TS, Medeiros YS. Animal models to test drugs with potential antidiabetic activity. J Ethnopharmacol. 2008;115(2):173-83.
[11]Bell RH Jr, Hye RJ. Animal models of diabetes mellitus: Physiology and pathology. J Surg Res. 1983;35(5):433-60.
[12]Houcher Z, Boudiaf K, Benboubetra M, Houcher B. Effects of methanolic extract and commercial oil of Nigella sativa L. on blood glucose and antioxidant capacity in alloxan-induced diabetic rats. Pteridines. 2007;18(1):8-18.
[13]Hawsawi ZA, Ali BA, Bamosa AO. Effect of Nigella sativa (black seed) and thymoquinone on blood glucose in albino rats. Ann Saudi Med. 2001;21(3-4):242-4.
[14]Bhat M, Zinjarde SS, Bhargava SY, Kumar AR, Joshi BN. Antidiabetic Indian plants: A good source of potent amylase inhibitors Evid Based Complement Alternat Med. 2011;2011:810207.
[15]Luo JZ, Luo L. Ginseng on hyperglycemia: effects and mechanisms. Evid Based Complement Alternat Med. 2009;6(4):423-7.
[16]Samane S, Noel J, Charrouf Z, Amarouch H, Haddad PS. Insulin-sensitizing and anti-proliferative effects of Argania spinosa seed extracts. Evid Based Complement Alternat Med. 2006;3(3):317-27.
[17]Meddah B, Ducroc R, El Abbes Faouzi M, Eto B, Mahraoui L, Benhaddou-Andaloussi A, et al. Nigella sativa inhibits intestinal glucose absorption and improves glucose tolerance in rats. J Ethnopharmacol. 2009;121(3):419-24.
[18]Fararh K, Atoji Y, Shimizu Y, Shiina T, Nikami H, Takewaki T. Mechanisms of the hypoglycaemic and immunopotentiating effects of Nigella sativa L. oil in streptozotocin-induced diabetic hamsters. Res Vet Sci. 2004;77(2):123-9.
[19]Benhaddou-Andaloussi A, Martineau L, Vuong T, Meddah B, Madiraju P, Settaf A, et al. The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content. Evid Based Complement Alternat Med. 2011;2011:538671
[20]Al-Logmani A, Zari T. Long-term effects of Nigella sativa L. oil on some physiological parameters in normal and streptozotocin-induced diabetic rats. J Diabetes Mellitus. 2011;1(3):46-53.
[21]Saleh DO, Bayoumi AR, El-Eraky WI, El-Khatib AS. Streptozotocin-induced vascular and biochemical changes in rats: Effects of rosiglitazone vs. metformin. Bull Faculty Pharm Cairo Univ. 2013;51(2):131-8.
[22]Beisswenger P, Ruggiero-Lopez D. Metformin inhibition of glycation processes. 2003;29(4 Pt 2):6S95-103
[23]Klip A, Leiter LA. Cellular mechanism of action of metformin. Diabetes Care. 1990;13(6):696-704.
[24]Zhou G1, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, Wu M, et al. Role of AMP-activated protein kinase in mechanism of metformin action. J Clin Invest. 2001;108(8):1167-74.
[25]Kannel WB. Lipids, diabetes, and coronary heart disease: Insights from the Framingham Study. Am Heart J. 1985;110(5):1100-7.
[26]Kaleem M, Kirmani D, Asif M, Ahmed Q, Bano B. Biochemical effects of Nigella sativa L seeds in diabetic rats. Indian J Exp Biol. 2006;44(9):745-8.
[27]Al-Naqeep G, Al-Zubairi AS, Ismail M, Amom ZH, Esa NM. Antiatherogenic potential of Nigella sativa seeds and oil in diet-induced hypercholesterolemia in rabbits. Evid Based Complement Alternat Med. 2011;2011:213628.
[28]Hadjzadeh M-AL-R , Pilavarian AA, Hydari AA, Behnam Rassouli F. The effect of aqueous extract of Niglla sativa L. Seeds in streptozocin induced diabetic rat. Pharmacologyonline. 2008;3:986-91.
[29]Najmi A, Nasiruddin M, Khan RA, Haque SF. Effect of Nigella sativa oil on various clinical and biochemical parameters of insulin resistance syndrome. Int J Diabetes Dev Ctries. 2008;28(1):11-4.
[30]Pentikainen PJ, Voutilainen E, Aro A, Uusitupa M, Penttila I, Vapaatalo H. Cholesterol lowering effect of metformin in combined hyperlipidemia: placebo controlled double blind trial. Ann Med. 1990;22(5):307-12.