@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(1):31-36
ISSN: 2252-0805 The Horizon of Medical Sciences 2015;21(1):31-36
Effect of Hydroalcoholic Extract of Prosopis farcta Pod on Liver Histopathology and Malondialdehyde Level in Streptozotocin Diabetic Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Hajinezhad M.R. (* )Esmaeel Zadeh Bahabadi S. (1 )
Miri H.R. (2 )
Davari S.A. (3 )
Darvish Sargazi M. (1)
(* ) Basic Sciences Department, Veterinary Medicine Faculty, University of Zabol, Zabol, Iran
(1 ) Biology Department, Basic Sciences Faculty, University of Zabol, Zabol, Iran
(2 ) Basic Sciences Department, Medicine Faculty, Torbat Heydarieh University of Medical Sciences, Torbat Heydarieh, Iran
(3 ) Pathobiology Department, Veterinary Medicine Faculty, University of Zabol, Zabol, Iran
Correspondence
Address: Veterinary Medicine Faculty, University of Zabol, Kilometer 2 nd of Bonjar-Pardis Road, Zabol, Iran. Postal Code: 98613-35856Phone: +9854322326567
Fax: +9854322240735
hajinezhad@uoz.ac.ir
Article History
Received: November 12, 2014Accepted: February 23, 2015
ePublished: April 16, 2015
ABSTRACT
Aims
Diabetes is a common endocrine disorder that can lead to hyperglycemia
and hyperlipidemia. The aim of this study was to investigate the effect of
hydroalcoholic extract of pod Prosopis fracta, on liver histopathology and tissue
level of malondialdehyde (MDA) in streptozotocin-induced diabetic rats.
Materials & Methods 45 male Wistar rats (200-300g) were divided into 3 groups; control, diabetic and Prosopis farcta extract treated diabetic. Type 1 diabetes was induced in by injection of streptozotocin (42mg/kg). One week after diabetes induction, Prosopis farcta extract (300mg/kg of body weight) was administered to treated diabetic group by gavage for 30 days. Hepatic histological changes were assessed with Hematoxylin-Eosin staining under light microscopy. The liver concentration of MDA was determined as thiobarbituric acid reactive substances (TBARS). The obtained data were statistically analyzed using Students T and Mann-Whitney rank sum tests.
Findings Administration of Prosopis fracta extract decreased the concentration of malondialdehyde in liver tissue of treated diabetic group in comparison to diabetic group significantly (p<0.05). Inflation and vacuolation of hepatocytes were observed with disarrangement of hepatic cords and sinusoidal narrowing. All previous signs were improved in Prosopis fracta treated group.
Conclusion Hydro-alcoholic extract of Prosopis fracta pod can reduce the level of malondialdehyde as a marker of lipid peroxidation in liver and prevent the histopathological changes of liver associated with diabetes.
Materials & Methods 45 male Wistar rats (200-300g) were divided into 3 groups; control, diabetic and Prosopis farcta extract treated diabetic. Type 1 diabetes was induced in by injection of streptozotocin (42mg/kg). One week after diabetes induction, Prosopis farcta extract (300mg/kg of body weight) was administered to treated diabetic group by gavage for 30 days. Hepatic histological changes were assessed with Hematoxylin-Eosin staining under light microscopy. The liver concentration of MDA was determined as thiobarbituric acid reactive substances (TBARS). The obtained data were statistically analyzed using Students T and Mann-Whitney rank sum tests.
Findings Administration of Prosopis fracta extract decreased the concentration of malondialdehyde in liver tissue of treated diabetic group in comparison to diabetic group significantly (p<0.05). Inflation and vacuolation of hepatocytes were observed with disarrangement of hepatic cords and sinusoidal narrowing. All previous signs were improved in Prosopis fracta treated group.
Conclusion Hydro-alcoholic extract of Prosopis fracta pod can reduce the level of malondialdehyde as a marker of lipid peroxidation in liver and prevent the histopathological changes of liver associated with diabetes.
CITATION LINKS
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[12]Tehranipour M, Mollashahi M, Javadmoosavi BZ. Effect of ethanolic extract of pod Prosopis farcta plant on neuronal density of anterior horn following Sciatic nerve compression in Rats. J Gorgan Uni Med Sci. 2012;14(4):39-43. [Persian]
[13]Ranjbar-Heidari A, Khaiatzadeh J, Mahdavi-Shahri N, Tehranipoor M. The effect of fruit pod powder and aquatic extract of prosopis farcta on healing cutaneous wounds in diabetic Rat. Zahedan J Res Med Sci. 2012;14(5):16-20.
[14]Al-Aboudi A, Afifi FU. Plants used for the treatment of diabetes in Jordan: A review of scientific evidence. Pharm Biol. 2011;49(3): 221-39.
[15]Hedayati M, Pouraboli I, Pouraboli B, Dabiri Sh, Javadi A. Effects of otostegia persica extract on serum level of glucose and morphology of pancreas in diabetic rats. Koomeh. 2012;13(2):201-8. [Persian]
[16]Zare T, Mokhtari M, Mohammadi J.The effect of Hydroalcoholic extracts of prangos ferulacea on blood factors of kidney and liver functions in diabetic male wistar rats. J Fasa Univ of Med Sci. 2012;2(3):174-80. [Persian]
[17]Rahbarian R, Sadooghi SD. Investigating the effects of aqueous extract of asafoetida resin on the serum level of insulin and blood glucose in type 1 diabetic rats. J Shahrekord Univ Med Sci. 2014;16(3):16-21. [Persian]
[18]Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: Reference interval and effects of lifestyle factors. Clin Chem. 1997;43(7):1209-14.
[19]Masjedi F, Gol A, Dabiri Sh, Javadi A. Preventive effect of garlic on histopathology of liver and markers of hepatic injury in streptozotocin-induced diabetic rats. Iran J Endocrinol Metabolism. 2009;11(4):433-41. [Persian]
[20]Sies H. Strategies of antioxidant defense. Eur J Biochem. 1993;215(2):213-9.
[21]Ali-Shtayeh MS, Jamous RM, Al-Shafie' JH, Elgharabah WA, Kherfan FA, Qarariah KH. Traditional knowledge of wild edible plants used in Palestine (Northern West Bank): A comparative study. J Ethnobiol Ethnomed. 2008;12(4):13.
[22]Al-Jeboory AA, Alhusainy WAH. Cardiovascular studies on prosopis farcta. Fitoterapia. 1984;55:137-42.
[23]Yaniv Z, Dafni A, Friedman B, Palevith D. Plants used for the treatment of diabetes in Israel. J Ethnopharmacol. 1987;19(2):145-51.
[24]Afifi FU. Hypoglycemic effects of prosopis farcta. Int J Pharmacognosy. 1993;31(2):161-4.
[25]Asadollahi K, Abassi N, Afshar N, Alipour M, Asadollafi p.. Investigation of the effects of prosopis farcta plant extract on rat's aorta. J Med Plants Res. 2010;4(2):142-7.
[26]Asadollahi A, Sarir H, Omidi A, Torbati MB. Hepatoprotective potential of prosopis farcta beans extracts against acetaminophen-induced Hepatotoxicity in wister rats. Int J Prev Med. 2014;5(10):1281-5
[27]Jafari F, Minaiyan M, Hoseyni-Baharanchi M, Heidari- Beni M. Anti-diabetic effect of prosopis farcta (Bank & Soland) J. F. Macbar extract in rats. J Health Syst Res. 2013;Special Issue on Nutrition:1649-56. [Persian]
[2]Widlansky ME, Gutterman DD. Regulation of endothelial function by mitochondrial reactive oxygen species. Antioxid Redox Signal. 2011;15(6):1517–30.
[3]Naudi A, Jove M, Ayala V, Cassanye A, Serrano J, Gonzalo H, et al. Cellular dysfunction in diabetes as maladaptive response to mitochondrial oxidative stress. Exp Diabetes Res. 2012;2012:1-14 .
[4]Victor VM, Rocha M, Herance R, Hernandez-Mijares A. Oxidative stress and mitochondrial dysfunction in type 2 diabetes. Curr Pharm Des. 2011;17(36):3947-58.
[5]Esrefoglu M. Oxidative stress and benefits of antioxidant agents in acute and chronic hepatitis. Hepat Mon. 2012;12(3):160-7.
[6]Ryan EA, Pick ME, Marceau C. Use of alternative medicines in diabetes mellitus. Diabet Med. 2001; 18(3):242-5.
[7]Harzallah-Skhiri F, Ben Jannet H. Flavonoids diversification in organs of two prosopis farcta (Banks & Sol.) Eig. (Leguminosea, Mimosoideae) populations occurring in the northeast and the southeast of Tunisia. J Appl Sci Res. 2005;1(2):130-6.
[8]Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine. 1996;2(2):137- 89.
[9]Ansari nik H, Saberi M, Jahantigh M, Ebrahimzadeh A. The evaluation of chemical composition and dry matter degradability of prosopis farcta fruit using in situ nylon bag technique. Int J Agric Crop Sci. 2013;5(9)972-5.
[10]Molan AL, Mahdy AS. Iraqi medicinal plants: Total flavonoid contents, free-radical scavenging and bacterial beta-glucuronidase inhibition activities. IOSR J Dental and Med Sci. 2014;13(5)72-7.
[11]Duthie G, Crozier A. Plant-derived phenolic antioxidants. Curr Opin Clin Nutr Metab Care. 2000; 3(6):447-51.
[12]Tehranipour M, Mollashahi M, Javadmoosavi BZ. Effect of ethanolic extract of pod Prosopis farcta plant on neuronal density of anterior horn following Sciatic nerve compression in Rats. J Gorgan Uni Med Sci. 2012;14(4):39-43. [Persian]
[13]Ranjbar-Heidari A, Khaiatzadeh J, Mahdavi-Shahri N, Tehranipoor M. The effect of fruit pod powder and aquatic extract of prosopis farcta on healing cutaneous wounds in diabetic Rat. Zahedan J Res Med Sci. 2012;14(5):16-20.
[14]Al-Aboudi A, Afifi FU. Plants used for the treatment of diabetes in Jordan: A review of scientific evidence. Pharm Biol. 2011;49(3): 221-39.
[15]Hedayati M, Pouraboli I, Pouraboli B, Dabiri Sh, Javadi A. Effects of otostegia persica extract on serum level of glucose and morphology of pancreas in diabetic rats. Koomeh. 2012;13(2):201-8. [Persian]
[16]Zare T, Mokhtari M, Mohammadi J.The effect of Hydroalcoholic extracts of prangos ferulacea on blood factors of kidney and liver functions in diabetic male wistar rats. J Fasa Univ of Med Sci. 2012;2(3):174-80. [Persian]
[17]Rahbarian R, Sadooghi SD. Investigating the effects of aqueous extract of asafoetida resin on the serum level of insulin and blood glucose in type 1 diabetic rats. J Shahrekord Univ Med Sci. 2014;16(3):16-21. [Persian]
[18]Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: Reference interval and effects of lifestyle factors. Clin Chem. 1997;43(7):1209-14.
[19]Masjedi F, Gol A, Dabiri Sh, Javadi A. Preventive effect of garlic on histopathology of liver and markers of hepatic injury in streptozotocin-induced diabetic rats. Iran J Endocrinol Metabolism. 2009;11(4):433-41. [Persian]
[20]Sies H. Strategies of antioxidant defense. Eur J Biochem. 1993;215(2):213-9.
[21]Ali-Shtayeh MS, Jamous RM, Al-Shafie' JH, Elgharabah WA, Kherfan FA, Qarariah KH. Traditional knowledge of wild edible plants used in Palestine (Northern West Bank): A comparative study. J Ethnobiol Ethnomed. 2008;12(4):13.
[22]Al-Jeboory AA, Alhusainy WAH. Cardiovascular studies on prosopis farcta. Fitoterapia. 1984;55:137-42.
[23]Yaniv Z, Dafni A, Friedman B, Palevith D. Plants used for the treatment of diabetes in Israel. J Ethnopharmacol. 1987;19(2):145-51.
[24]Afifi FU. Hypoglycemic effects of prosopis farcta. Int J Pharmacognosy. 1993;31(2):161-4.
[25]Asadollahi K, Abassi N, Afshar N, Alipour M, Asadollafi p.. Investigation of the effects of prosopis farcta plant extract on rat's aorta. J Med Plants Res. 2010;4(2):142-7.
[26]Asadollahi A, Sarir H, Omidi A, Torbati MB. Hepatoprotective potential of prosopis farcta beans extracts against acetaminophen-induced Hepatotoxicity in wister rats. Int J Prev Med. 2014;5(10):1281-5
[27]Jafari F, Minaiyan M, Hoseyni-Baharanchi M, Heidari- Beni M. Anti-diabetic effect of prosopis farcta (Bank & Soland) J. F. Macbar extract in rats. J Health Syst Res. 2013;Special Issue on Nutrition:1649-56. [Persian]