ARTICLE INFO

Article Type

Original Research

Authors

Payehdar   A. (1 )
Hosseini   S.E. (* )
Mehrabani   D. (2)
Forouzanfar   M. (3 )






(* ) Biology Department, Science Faculty, Marvdasht Branch,Islamic Azad University, Shiraz, Iran
(1 ) Biology Department, Science Faculty, Fars Science and Research Branch,Islamic Azad University, Shiraz, Iran
(2) Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
(3 ) Biology Department , Science Faculty, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

Correspondence

Address: Science Faculty, Shiraz Branch, Islamic Azad University, Kilometer 5 of Sadra Road, Shiraz, Iran. Postal Code: 71886-566575
Phone: +98 (71) 43311148
Fax: +98 (71) 43311172
ebrahim.hossini@yahoo.com

Article History

Received:   December  8, 2016
Accepted:   January 30, 2017
ePublished:   July 22, 2017

ABSTRACT

Aims Conditioned medium of mesenchymal stem cells have a positive effect on repairing damaged tissues because of having metabolites, growth factors and cytokines. The aim of current study was to investigate the healing effect of conditioned medium of adipose tissue-derived stem cells on histomorphometric changes of testis in treated mice with busulfan.
Materials & Methods In this experimental study, 18 adult male mice were divided into the three equal groups including control group (Without treatment), azoospermia sham and experimental groups. The sham and experimental groups were induced azoospermia by two injection of 10mg/kg busulfan intraperitoneally, with 21 days interval. 35 days after the second injection of busulfan, the experimental group treated with conditioned medium of adipose tissue-derived stem cells, and 60 days after conditioned medium injection, the testes of mice were removed and histomorphometric evaluation was done. The results were analyzed by SPSS 18 software using Mann-Whitney, ANOVA and Tukey's post hoc tests.
Findings The lumen diameter and area of the seminiferous tubules in the three groups did not have any significant difference; however, the total diameter, cellular diameter, cellular area, cross-sectional area and spermatogenesis index of the seminiferous tubules were significantly decreased in the experimental group compared to the control group (p<0.05), while they were significantly increased compared to the azoospermia sham group (p<0.001). Also, the number of tubules and numerical density of the seminiferous tubules were significantly increased in the azoospermia sham compared to the control and experimental groups (p<0.001).
Conclusion Secretory factors of conditioned medium from adipose tissue-derived stem cells could be effective in the restoration of spermatogenesis in busulfan-induced infertile mice.

Keywords: Azoospermia, Adipose Tissue , Mice , Culture Media, Conditioned , Mesenchymal Stromal Cells ,

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