ARTICLE INFO

Article Type

Original Research

Authors

Habibian   M. (*)
Monavari   M. (1)






(*) Sports Sciences Department, Humanities Faculty, Qaemshahar Branch, Islamic Azad University, Qaemshahar, Iran
(1) Sports Sciences Department, Sari Branch, Islamic Azad University, Sari , Iran

Correspondence

Address: Humanities Faculty, Islamic Azad University, Allameh Tabarsi Street, Qaemshahar, Iran. Postal Code: 475161964
Phone: +98 (11) 42155025
Fax: +98 (11) 42155117
habibian_m@yahoo.com

Article History

Received:   May  2, 2016
Accepted:   January 16, 2017
ePublished:   March 25, 2017

ABSTRACT

Aims Severe exercises lead to higher blood homocysteine levels, as well as heat shock protein 72 (HSP72). The aim of the study was to compare homocysteine and HSP72 responses following moderate-intensity continuous and high-intensity interval exercises in the sedentary women.
Materials & Methods In the semi-experimental study, 20 sedentary and healthy young female students were studied in Islamic Azad University, Sari Branch, in 2014. The subjects, selected via purposeful and available sampling method, were randomly divided into two groups including continuous exercise and high-intensity interval exercise (n=10 persons per group). 40-minute running on the treadmill with 60-65% of maximum consumed oxygen was conducted in continuous group. In addition, 36-minute running on the treadmill was conducted in interval group through alternate sets including 1 minute with 90-95% of maximum consumed oxygen and 3 minutes with 50% of maximum consumed oxygen. Blood sampling was done following 12-hour night fasting at 3 stages including before, immediately after, and 60 minutes after the exercises. Data was analyzed by SPSS 20 software using repeated ANOVA, LSD post-hoc, and independent T tests.
Findings Both severe exercises significantly increased homocysteine and HP72 levels (p<0.05), decreasing after 1-hour recovery compared to the interval immediately after the exercise. Nevertheless, HSP72 levels remained significantly higher than the basic levels (p<0.05).
Conclusion Both moderate-intensity continuous and high-intensity interval exercises similarly change homocysteine and HSP72 levels in the sedentary women.

Keywords: Exercise, Heat Shock Protein 72, Homocysteine,

CITATION LINKS

[1]Venta R, Cruz E, Valcárcel G, Terrados N. Plasma vitamins, amino acids, and renal function in postexercise hyperhomocysteinemia. Med Sci Sports Exerc. 2009;41(8):1645-51.
[2]Borrione P, Rizzo M, Spaccamiglio A, Salvo RA, Dovio A, Termine A, et al. Sport-related hyperhomocysteinaemia: A putative marker of muscular demand to be noted for cardiovascular risk. Br J Sports Med. 2009;42(11):894-900.
[3]Hammouda O, Chtourou H, Chaouachi A, Chahed H, Ferchichi S, Kallel C, et al. Effect of short-term maximal exercise on biochemical markers of muscle damage, total antioxidant status, and homocysteine levels in football players. Asian J Sports Med. 2012;3(4):239-46.
[4]Sutken E, Akalin A, Ozdemir F, Colak O. Lipid profile and levels of homocysteine, leptin, fibrinogen and C-reactive protein in hyperthyroid patients before and after treatment. Dicle Med J. 2010;37(1):1-7.
[5]Joubert LM, Manore MM. Exercise, nutrition, and homocysteine. Int J Sport Nutr Exerc Metab. 2006;16(4):341-61.
[6]Unt E, Zilmer K, Magi A, Kullisaar T, Kairane C, Zilmer M. Homocysteine status in former top-level male athletes: possible effect of physical activity and physical fitness. Scand J Med Sci Sports. 2008;18(3):360-6.
[7]Dankner R, Geulayov G, Farber N, Novikov I, Segev S, Sela BA. Cardiorespiratory fitness and plasma homocysteine levels in adult males and females. Isr Med Assoc J. 2009;11(2):78-82.
[8]Ruiz JR, Hurtig-Wennlöf A, Ortega FB, Patterson E, Nilsson TK, Castillo MJ, et al. Homocysteine levels in children and adolescents are associated with the methylenetetrahydrofolate Reductase 677C > T genotype, but not with physical activity, fitness or fatness: The European youth heart study. Br J Nutr. 2007;97(2):255-62.
[9]Hayward R, Ruangthai R, Karnilaw P, Chicco A, Strange R, McCarty H, et al. Attenuation of homocysteine-induced endothelial dysfunction by exercise training. Pathophysiology. 2003;9(4):207-14.
[10]Walsh RC, Koukoulas I, Garnham A, Moseley L, Hargreaves M, Febbraio MA. Exercise increases serum Hsp72 in humans. Cell Stress Chaperones. 2001;6(4):386-93.
[11]Melo SF, Lunz W, Fontes EP, Dias CM, Carneiro MA Jr, Moura AG, et al. Different levels of Hsp72 in female rat myocardium in response to voluntary exercise and forced exercise. Arq Bras Cardiol. 2009;93(5):456-62.
[12]Febbraio MA, Ott P, Nielsen HB, Steensberg A, Keller C, Krustrup P, et al. Exercise induces hepatosplanchnic release of heat shock protein 72 in humans. J Physiol. 2002;544(Pt 3):957-62.
[13]Fehrenbach EA, Niess M ,Voelker K, Northoff H, Mooren FC. Exercise Intensity and Duration Affect Blood Soluble HSP72. Int J Sports Med. 2005;26(7):552-7.
[14]Fallon K, Fallon S, Boston T. The acute phase response and exercise: court and field sports. Br J Sports Med. 2001;35(3):170-3.
[15]Haskell WL, Lee IM, Pate RR, Powell KE, Blair SN, Franklin BA, et al. Physical activity and public health: Updated recommendation for adults from the American College of Sports Medicine and the American Heart Association. Circulation. 2007;116(9):1081–93.
[16]Helgerud J, Hoydal K, Wang E, Karlsen T, Berg P, Bjerkaas M, et al. Aerobic high intensity intervals improve VO2max more than moderate training. Med Sci Sports Exerc. 2007;39(4):665-71.
[17]Skidmore BL, Jones MT, Blegen M, MatthewS TD. Acute effects of three different circuit weight training protocols on blood lactate, heart rate, and rating of perceived exertion in recreationally active women. J Sports Sci Med. 2012;11(4):660-8.
[18]Meyer P, Gayda M, Juneau M, Nigam A. High-intensity aerobic interval exercise in chronic heart failure. Curr Heart Fail Rep. 2013;10(2):130-8.
[19]Jackson AS, Pollock ML, Ward A. Generalized equations for predicting body density of women. Med Sci Sports Exerc. 1980;12(3):175-81.
[20]Sim AY, Wallman KE, Fairchild TJ, Guelfi KJ. High-intensity intermittent exercise attenuates ad-libitum energy intake. Int J Obes (Lond). 2014;38(3):417-22.
[21]Ueland PM, Refsum H, Stabler SP, Malinow MR, Andersson A, Allen RH. Total homocysteine in plasma or serum: methods and clinical applications. Clin Chem. 1993;39(9):1764-79.
[22]Iglesias-Gutiérrez E, Egan B, Diaz-Martínez AE, Penalvo JL, Gonzalez-Medina A, Martinez-Camblor P, et al. Transient increase in homocysteine but not hyperhomocysteinemia during acute exercise at different intensities in sedentary individuals. PLoS One. 2012;7(12):e51185.
[23]Murawska-Ciałowicz E. The impact of Wingate and progressive tests on homocysteine, vitamin B6, B12 and folic acid levels in athletes’ blood. Centr Eur J Sport Sci Med. 2014;8(4):5-17.
[24]Subasi SS, Gelecek N, Ozdemir N, Orman M. Influences of acute resistance and aerobic exercises on plasma homocysteine level and lipid profiles. Turk J Biochem. 2009;34(1):9-14.
[25]Gelecek N, Teoman N, Ozdirenc M, Pinar L, Akan P, Bediz C, et al. Influences of acute and chronic aerobic exercise on the plasma homocysteine level. Ann Nutr Metab. 2007;51(1):53-8.
[26]Gaume V, Mougin F, Figard H, Simon-Rigaud ML, Nguyen UN, Callier J, et al. Physical training decreases total plasma homocysteine and cysteine in middle-aged subjects. Ann Nutr Metab. 2005;49(2):125-31.
[27]Deminice R, Ribeiro DF, Frajacomo FT. The Effects of Acute Exercise and Exercise Training on Plasma Homocysteine: A Meta-Analysis. PLoS One. 2016;11(3):e0151653.
[28]Kregel KC. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J Appl Physiol (1985). 2002;92(5):2177-86.
[29]Beckmann RP, Mizzen LE, Welch WJ. Interaction of hsp 70 with newly synthesized: Implications for protein folding and assembly. Science. 1990;248(4957):850-4.
[30]Asea A, Kabingu E, Stevenson M A, Calderwood SK. HSP70 peptidembearing and peptide- negative preparations act as chaperokines. Cell Stress Chaperones. 2000;5(5):425-31.
[31]Fehrenbach E, Passek F, Niess AM, Pohla H, Weinstock C, Dickhuth HH, et al. HSP expression in human leukocytes is modulated by endurance exercise. Med Sci Sports Exerc. 2000;32(3):592-600.
[32]Peake JM, Suzuki K, Hordern M, Wilson G, Nosaka K, Coombes JS. Plasma cytokine changes in relation to exercise intensity and muscle damage. Eur J Appl Physiol. 2005;95(5-6):514-21.
[33]Khajehlandi A, Jafarei H, Mohammadi Demieh A, Barzideh P. The effect of aerobic and anaerobic tests on the alternation of heat shock proteins levels on young female. Arak Med Univ J. 2013;16(5):19-27. [Persian]
[34]Nameni F. The effect of one bout endurance and acute exercise on HSP 70 in female. World Appl Sci J. 2012;18(5):727-30.
[35]Sadowska-Krepa E, Klapcinska B. Exercise-induced heat shock protein (HSP70) response in human skeletal muscle and leukocytes. Med Sportiva. 2006;10(2):36-41.
[36]Febbraio MA, Mesa JL, Chung J, Steensberg A, Keller C, Nielsen HB, et al. Glucose ingestion attenuates the exercise-induced increase in circulating heat shock protein 72 and heat shock protein 60 in humans. Cell Stress Chaperones. 2004;9(4):390-6.
[37]Heck TG, Scholer CM, de Bittencourt PI. HSP70 expression: does it a novel fatigue signalling factor from immune system to the brain?. Cell Biochem Funct. 2011;29(3):215-26.