@2024 Afarand., IRAN
ISSN: 2252-0805 The Horizon of Medical Sciences 2016;22(4):337-344
ISSN: 2252-0805 The Horizon of Medical Sciences 2016;22(4):337-344
Effect of Hydroalcholic Extract of Curcuma longa on Adriamycin-Induced Renal Damage in Rats
ARTICLE INFO
Article Type
Original ResearchAuthors
Mohebbati R. (1)Abbasnezhad A.A. (2)
Khajavi Rad A. (*)
Haghshenas M. (1)
Khazdeir M.R. (1)
(*) Physiology Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(1) Physiology Department, Medicine Faculty, Mashhad University of Medical Sciences, Mashhad, Iran
(2) Basic Sciences Department, Medicine Faculty, Gonabad University of Medical Sciences, Gonabad, Iran
Correspondence
Address: Department of Physiology, School of Medicine, Azadi Square, Mashhad, Iran. Postal Code: 917794-8564Phone: +98 (51) 38828565
Fax: +98 (51) 38828564
khajavirada@mums.ac.ir
Article History
Received: January 12, 2015Accepted: May 11, 2016
ePublished: January 10, 2016
ABSTRACT
Aims
Adriamycin is one of the anti-cancer medications. Nevertheless, the medication causes renal damage. Curcuma longa has anti-inflammatory and anti-oxidant effects. The aim of this study was to determine the effects of hydroalcoholic extract of Curcuma longa on renal damage due to Adriamycin in the rat.
Materials & Methods In the experimental study, 32 male Wistar rats were studied. Via simple random method, the rats were divided into four groups including control, Adriamycin (5mg/Kg), Curcuma longa extract (1000mg/Kg), and Curcuma longa extract with Adriamycin groups. The groups underwent 5-week treatment. 24-hour urine samples were collected at days 0, 6, 10, 14, 21, 28, and 35, to measure glomerular filtration rate. The left kidney of the animal was used to determine the renal damage percentage. Data was analyzed by SPSS 16 software using one-way ANOVA, Tukey’s post-hoc, and paired T tests.
Findings Mean of glomerular filtration rate in Adriamycin group significantly decreased at days 21 and 35 compared to days 0 and 6. In addition, it significantly increased in Curcuma longa extract group at days 10, 14, 21, and 28 (p<0.05). The renal damage percentage significantly increased in Adriamycin (p<0.001) and Curcuma longa extract with Adriamycin (p<0.01) groups than control group. In addition, it significantly decreased in Curcuma longa extract group, as well as Curcuma longa extract with Adriamycin group, compared to Adriamycin group (p<0.001).
Conclusion Hydroalcoholic extract of Curcuma longa reduces the renal damage percentage caused by Adriamycin in the rat, while improves glomerular filtration rate.
Materials & Methods In the experimental study, 32 male Wistar rats were studied. Via simple random method, the rats were divided into four groups including control, Adriamycin (5mg/Kg), Curcuma longa extract (1000mg/Kg), and Curcuma longa extract with Adriamycin groups. The groups underwent 5-week treatment. 24-hour urine samples were collected at days 0, 6, 10, 14, 21, 28, and 35, to measure glomerular filtration rate. The left kidney of the animal was used to determine the renal damage percentage. Data was analyzed by SPSS 16 software using one-way ANOVA, Tukey’s post-hoc, and paired T tests.
Findings Mean of glomerular filtration rate in Adriamycin group significantly decreased at days 21 and 35 compared to days 0 and 6. In addition, it significantly increased in Curcuma longa extract group at days 10, 14, 21, and 28 (p<0.05). The renal damage percentage significantly increased in Adriamycin (p<0.001) and Curcuma longa extract with Adriamycin (p<0.01) groups than control group. In addition, it significantly decreased in Curcuma longa extract group, as well as Curcuma longa extract with Adriamycin group, compared to Adriamycin group (p<0.001).
Conclusion Hydroalcoholic extract of Curcuma longa reduces the renal damage percentage caused by Adriamycin in the rat, while improves glomerular filtration rate.
CITATION LINKS
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[2]Balakumar P, Chakkarwar VA, Kumar V, Jain A, Reddy J, Singh M. Experimental models for nephropathy. J Ren Angiotensin Aldosterone Syst. 2008;9(4):189-95.
[3]Mohebati R, Abbasnezhad AA, Khajavi Rad A, Mosavi M, Haghshenas M. The effects of hydroalcholic extract of Nigella sativa on doxorubicin-induced renal functional damage in rats. Horizon Medi Sci. 2015;21(4):1-10. [Persian]
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[8]Akram M, Shahab-Uddin AA, Usmanghani K, Hannan A, Mohiuddin E, Asif M. Curcuma longa and curcumin: A review article. Rom J Biol. 2010;55(2):65-70.
[9]Huang MT, Wang ZY, Georgiadis CA, Laskin JD, Conney AH. Inhibitory effects of curcumin on tumor initiation by benzo [a] pyrene and 7, 12-dimethylbenz [a] anthracene. Carcinog. 1992;13(11):2183-6.
[10]Ramirez-Tortosa MC, Mesa MD, Aguilera MC, Quiles JL, Baro L, Ramirez-Tortosa CL, et al. Oral administration of a turmeric extract inhibits LDL oxidation and has hypocholesterolemic effects in rabbits with experimental atherosclerosis. Atheroscler. 2000;147(2):371-8.
[11]Nishiyama T, Mae T, Kishida H, Tsukagawa M, Mimaki Y, Kuroda M, et al. Curcuminoids and sesquiterpenoids in turmeric (Curcuma longa L.) suppress an increase in blood glucose level in type 2 diabetic KK-Ay mice. J Agric Food Chem. 2005;53(4):959-63.
[12]Antony S, Kuttan R, Kuttan G. Immunomodulatory activity of curcumin. Immunol Invest. 1999;28(5-6):291-303.
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[14]Araujo CAC, Leon LL. Biological activities of Curcuma longa L. Mem Inst Oswaldo Cruz. 2001;96(5):723-8.
[15]Zima T, Tesar V, Crkovska J, Stejskalová A, Platenik J, Teminova J, et al. ICRF-187 (dexrazoxan) protects from adriamycin-induced nephrotic syndrome in rats. Nephrol Dial Transplant. 1998;13(8):1975-9.
[16]Khorsandi L, Orazizadeh M. Protective effect of Curcuma longa extract on acetaminophen induced nephrotoxicity in mice. DARU. 2008;16(3):155-9.
[17]Chromý V, Rozkošná K, Sedlak P. Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems. Clin Chem Lab Med. 2008;46(8):1127-33.
[18]Goering PL, Fisher BR, Noren BT, Papaconstantinou A, Rojko JL, Marler RJ. Mercury induces regional and cell-specific stress protein expression in rat kidney. Toxicol Sci. 2000;53(2):447-57.
[19]Di Marco A, Arcamone F, Zunino F. Daunomycin (daunorubicin) and adriamycin and structural analogues: Biological activity and mechanism of action. In: Corcoran JW, Hahn FE, Snell JF, Arora KL, editors. Mechanism of Action of Antimicrobial and Antitumor Agents [Volume 3]. Berlin Heidelberg: Springer; 1975. pp. 101-28.
[20]Sarhan M, El Serougy H, Hussein AM, El-Dosoky M, Sobh MA, Fouad SA, et al. Impact of bone-marrow-derived mesenchymal stem cells on adriamycin-induced chronic nephropathy. Can J Physiol Pharmacol. 2014;92(9):733-43.
[21]Liang C-l, Wu J-b, Lai J-m, Ye S-f, Lin J, Ouyang H, et al. Protection effect of Zhen-Wu-Tang on adriamycin-induced nephrotic syndrome via inhibiting oxidative lesions and inflammation damage. Evid Based Complement Altern Med. 2014;2014:1-11.
[22]Zhu C, Xuan X, Che R, Ding G, Zhao M, Bai M, et al. Dysfunction of the PGC-1alpha-mitochondria axis confers adriamycin-induced podocyte injury. Am J Physiol Renal Physiol. 2014;306(12):1410-7.
[23]Wang Z, Liu JT, Sun WS, Li RP, Wang Y. Effect of Qufeng Tongluo Recipe on expression of desmin and CD2AP proteins in adriamycin-induced nephropathy rats: An experimental research. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014;34(2):203-8. [Chinese]
[24]
[25]Hommel E, Mathiesen ER, Aukland K, Parving HH. Pathophysiological aspects of edema formation in diabetic nephropathy. Kidney Int. 1990;38(6):1187-92.
[26]You H, Lu Y, Gui D, Peng A, Chen J, Gu Y. Aqueous extract of Astragali Radix ameliorates proteinuria in adriamycin nephropathy rats through inhibition of oxidative stress and endothelial nitric oxide synthase. J Ethnopharmacol. 2011;134(1):176-82.
[27]Franco R, Gut A, Ferrari-Spadotto A, Georgette J, Gavras I, Gavras H. Pressor mechanisms in adriamycin-induced nephropathy with hypertension in rats. Hypertension. 1994;23(Suppl 1):246-9.
[28]Kawamori T, Lubet R, Steele VE, Kelloff GJ, Kaskey RB, Rao CV, et al. Chemopreventive effect of curcumin, a naturally occurring anti-inflammatory agent, during the promotion/progression stages of colon cancer. Cancer Res. 1999;59(3):597-601.
[29]Wu X, An P, Ye B, Shi X, Dang H, Fu R, et al. Artemisinin ameliorated proteinuria in rats with adriamycin-induced nephropathy through regulating nephrin and podocin expressions. J Tradit Chin Med. 2014;34(1):63-8.
[30]Wei M, Sun W, He W, Ni L, Cai X, Cheng Z, et al. Qiguiyishen decoction reduced the accumulation of extracellular matrix in the kidneys of rats with adriamycin-induced nephropathy. J Tradit Chin Med. 2014;34(3):351-6.
[31]Mohan M, Kamble S, Satyanarayana J, Nageshwar M, Reddy N. Protective effect of Solanum torvum on Doxorubicin-induced hepatotoxicity in rats. Int J Drug Dev Res. 2011;3(3):131-8.
[32]Elsherbiny NM, El-Sherbiny M. Thymoquinone attenuates Doxorubicin-induced nephrotoxicity in rats: Role of Nrf2 and NOX4. Chem Biol Interact. 2014;223:102-8.
[33]Sharma OP. Antioxidant activity of curcumin and related compounds. Biochem Pharmacol. 1976;25(15):1811-2.
[34]Mohebbati R, Shafei MN, Soukhtanloo M, Mohammadian Roshan N, Khajavi Rad A, Anaeigoudari A, et al. Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney. Avicenna J Phytomed. 2016;6(1):86-94.
[35]Koul A, Shubrant S, Gupta P. Phytomodulatory potential of lycopene from Lycopersicum esculentum against doxorubicin induced nephrotoxicity. Indian J Exp Biol. 2013;51(8):635-45.
[2]Balakumar P, Chakkarwar VA, Kumar V, Jain A, Reddy J, Singh M. Experimental models for nephropathy. J Ren Angiotensin Aldosterone Syst. 2008;9(4):189-95.
[3]Mohebati R, Abbasnezhad AA, Khajavi Rad A, Mosavi M, Haghshenas M. The effects of hydroalcholic extract of Nigella sativa on doxorubicin-induced renal functional damage in rats. Horizon Medi Sci. 2015;21(4):1-10. [Persian]
[4]Saito TE. Adriamycin: A review of its use, and guidelines for administration. Cancer Nurs. 1978;1(2):169-74.
[5]Jeansson M, Björck K, Tenstad O, Haraldsson B. Adriamycin alters glomerular endothelium to induce proteinuria. J Am Soc Nephrol. 2009;20(1):114-22.
[6]Venkatesan N, Punithavathi D, Arumugam V. Curcumin prevents adriamycin nephrotoxicity in rats. Br J Pharmacol. 2000;129(2):231-4.
[7]Eigner D, Scholz D. Ferula asa-foetida and Curcuma longa in traditional medical treatment and diet in Nepal. J Ethnopharmacol. 1999;67(1):1-6.
[8]Akram M, Shahab-Uddin AA, Usmanghani K, Hannan A, Mohiuddin E, Asif M. Curcuma longa and curcumin: A review article. Rom J Biol. 2010;55(2):65-70.
[9]Huang MT, Wang ZY, Georgiadis CA, Laskin JD, Conney AH. Inhibitory effects of curcumin on tumor initiation by benzo [a] pyrene and 7, 12-dimethylbenz [a] anthracene. Carcinog. 1992;13(11):2183-6.
[10]Ramirez-Tortosa MC, Mesa MD, Aguilera MC, Quiles JL, Baro L, Ramirez-Tortosa CL, et al. Oral administration of a turmeric extract inhibits LDL oxidation and has hypocholesterolemic effects in rabbits with experimental atherosclerosis. Atheroscler. 2000;147(2):371-8.
[11]Nishiyama T, Mae T, Kishida H, Tsukagawa M, Mimaki Y, Kuroda M, et al. Curcuminoids and sesquiterpenoids in turmeric (Curcuma longa L.) suppress an increase in blood glucose level in type 2 diabetic KK-Ay mice. J Agric Food Chem. 2005;53(4):959-63.
[12]Antony S, Kuttan R, Kuttan G. Immunomodulatory activity of curcumin. Immunol Invest. 1999;28(5-6):291-303.
[13]Gautam SC, Gao X, Dulchavsky S. Immunomodulation by curcumin. Adv Exp Med Biol. 2007;595:321-41.
[14]Araujo CAC, Leon LL. Biological activities of Curcuma longa L. Mem Inst Oswaldo Cruz. 2001;96(5):723-8.
[15]Zima T, Tesar V, Crkovska J, Stejskalová A, Platenik J, Teminova J, et al. ICRF-187 (dexrazoxan) protects from adriamycin-induced nephrotic syndrome in rats. Nephrol Dial Transplant. 1998;13(8):1975-9.
[16]Khorsandi L, Orazizadeh M. Protective effect of Curcuma longa extract on acetaminophen induced nephrotoxicity in mice. DARU. 2008;16(3):155-9.
[17]Chromý V, Rozkošná K, Sedlak P. Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems. Clin Chem Lab Med. 2008;46(8):1127-33.
[18]Goering PL, Fisher BR, Noren BT, Papaconstantinou A, Rojko JL, Marler RJ. Mercury induces regional and cell-specific stress protein expression in rat kidney. Toxicol Sci. 2000;53(2):447-57.
[19]Di Marco A, Arcamone F, Zunino F. Daunomycin (daunorubicin) and adriamycin and structural analogues: Biological activity and mechanism of action. In: Corcoran JW, Hahn FE, Snell JF, Arora KL, editors. Mechanism of Action of Antimicrobial and Antitumor Agents [Volume 3]. Berlin Heidelberg: Springer; 1975. pp. 101-28.
[20]Sarhan M, El Serougy H, Hussein AM, El-Dosoky M, Sobh MA, Fouad SA, et al. Impact of bone-marrow-derived mesenchymal stem cells on adriamycin-induced chronic nephropathy. Can J Physiol Pharmacol. 2014;92(9):733-43.
[21]Liang C-l, Wu J-b, Lai J-m, Ye S-f, Lin J, Ouyang H, et al. Protection effect of Zhen-Wu-Tang on adriamycin-induced nephrotic syndrome via inhibiting oxidative lesions and inflammation damage. Evid Based Complement Altern Med. 2014;2014:1-11.
[22]Zhu C, Xuan X, Che R, Ding G, Zhao M, Bai M, et al. Dysfunction of the PGC-1alpha-mitochondria axis confers adriamycin-induced podocyte injury. Am J Physiol Renal Physiol. 2014;306(12):1410-7.
[23]Wang Z, Liu JT, Sun WS, Li RP, Wang Y. Effect of Qufeng Tongluo Recipe on expression of desmin and CD2AP proteins in adriamycin-induced nephropathy rats: An experimental research. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014;34(2):203-8. [Chinese]
[24]
[25]Hommel E, Mathiesen ER, Aukland K, Parving HH. Pathophysiological aspects of edema formation in diabetic nephropathy. Kidney Int. 1990;38(6):1187-92.
[26]You H, Lu Y, Gui D, Peng A, Chen J, Gu Y. Aqueous extract of Astragali Radix ameliorates proteinuria in adriamycin nephropathy rats through inhibition of oxidative stress and endothelial nitric oxide synthase. J Ethnopharmacol. 2011;134(1):176-82.
[27]Franco R, Gut A, Ferrari-Spadotto A, Georgette J, Gavras I, Gavras H. Pressor mechanisms in adriamycin-induced nephropathy with hypertension in rats. Hypertension. 1994;23(Suppl 1):246-9.
[28]Kawamori T, Lubet R, Steele VE, Kelloff GJ, Kaskey RB, Rao CV, et al. Chemopreventive effect of curcumin, a naturally occurring anti-inflammatory agent, during the promotion/progression stages of colon cancer. Cancer Res. 1999;59(3):597-601.
[29]Wu X, An P, Ye B, Shi X, Dang H, Fu R, et al. Artemisinin ameliorated proteinuria in rats with adriamycin-induced nephropathy through regulating nephrin and podocin expressions. J Tradit Chin Med. 2014;34(1):63-8.
[30]Wei M, Sun W, He W, Ni L, Cai X, Cheng Z, et al. Qiguiyishen decoction reduced the accumulation of extracellular matrix in the kidneys of rats with adriamycin-induced nephropathy. J Tradit Chin Med. 2014;34(3):351-6.
[31]Mohan M, Kamble S, Satyanarayana J, Nageshwar M, Reddy N. Protective effect of Solanum torvum on Doxorubicin-induced hepatotoxicity in rats. Int J Drug Dev Res. 2011;3(3):131-8.
[32]Elsherbiny NM, El-Sherbiny M. Thymoquinone attenuates Doxorubicin-induced nephrotoxicity in rats: Role of Nrf2 and NOX4. Chem Biol Interact. 2014;223:102-8.
[33]Sharma OP. Antioxidant activity of curcumin and related compounds. Biochem Pharmacol. 1976;25(15):1811-2.
[34]Mohebbati R, Shafei MN, Soukhtanloo M, Mohammadian Roshan N, Khajavi Rad A, Anaeigoudari A, et al. Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney. Avicenna J Phytomed. 2016;6(1):86-94.
[35]Koul A, Shubrant S, Gupta P. Phytomodulatory potential of lycopene from Lycopersicum esculentum against doxorubicin induced nephrotoxicity. Indian J Exp Biol. 2013;51(8):635-45.