@2024 Afarand., IRAN
ISSN: 2251-8215 Sarem Journal of Reproductive Medicine 2017;1(1):35-38
ISSN: 2251-8215 Sarem Journal of Reproductive Medicine 2017;1(1):35-38
27 weeks fetus with cardiomegaly, hydrocephaly and pathological Diagnosis of cytomegalovirus
ARTICLE INFO
Article Type
Case ReportAuthors
Hassani M. (*)Bagher K. (1)
(*) Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
(1) General Sarem Lab, Sarem Women’s Hospital, Tehran, Iran
Correspondence
Article History
Received: September 1, 2015Accepted: December 26, 2015
ePublished: February 15, 2016
ABSTRACT
Patient & Methods
Cytomegalovirus (CMV) is a member of the β-Herpes family, which produces cytomegalic inclusions in infected cells. This virus produces a syndrome similar to infectious mononucleosis. Presence of specific antibodies against Cytomegalovirus in persons ‘ serum shows that, people contact with CMV during their lifetime. The first encounter with this virus during pregnancy can cause abnormalities such as cardiomegaly, hydrocephalus, and even fetal death. This case report concerns a 27 weeks fetus from a 27 year old mother at Sarem Hospital. According to the pathologic findings of the samples stained with H&E and PAS methods, molecular tests were performed to detect Cytomegalovirus, and after DNA extraction from paraffin blocks obtained from different tissues of the fetus using Nested-PCR with two pairs of primers (CMTR1, CMTR2, CMTR3, and CMTR4), the presence of this virus was investigated in two stages.
Conclusion Regarding the healthy karyotype and the absence of genetic problems and molecular investigations, the main cause of the changes in the fetus, including cardiomegaly, hydrocephalus, and the cause of fetal death in the 27th week, is the presence of Cytomegalovirus.
Conclusion Regarding the healthy karyotype and the absence of genetic problems and molecular investigations, the main cause of the changes in the fetus, including cardiomegaly, hydrocephalus, and the cause of fetal death in the 27th week, is the presence of Cytomegalovirus.
CITATION LINKS
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[2]Jawetz MA. Medical Microbiology. Norouzi J, Translator. Tehran: Hayyan Publishing Cultural Institute; 2000. [Persian]
[3]Stagno S, Pass RF, Dworsky ME, Henderson RE, Moore EG, Walton PD, et al. Congenital cytomegalovirus infection: the relative importance of primery and recurrent maternal infections. N Engl J Med. 1982;306(16):945-9.
[4]Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988;239(4839):487-91.
[5]Schochetman G, Ou CY, Jones WK. Polymerase chain reaction. J Infect Dis. 1988;158(6):1154-7.
[6]Khan G, Kangro HO, Coates PJ, Heath RB. Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA. J Clin Pathol. 1991;44(5):360-5.
[7]Razzaque A, Jahan N, MC Weeney D, etal. Localization of DNA sequence analysis of the transforming domain (mtrII) of human cytomegalovirus. Proc Natl Acad Sci USA. 1988;85(15):5709-13.
[8]Cytomegalovirus (CMV) and Congenital CMV Infection [Internet]. Atlanta: Centers for Disease control and prevent; 1946. [updated 2017 dec 5; cited 2008 jun 1]. Avilable from: https://www.cdc.gov/cmv/overview.html.
[9]Pickring LK, Baker CF, Long SS, McMillan JA, editors. Red Book: 2006 Report of the Committee on Infectious Diseases. Elk Grove Village: American Academy of Pediatrics. 2006.
[10]Lo SK, Ip KW, Chan PK, Nicholls JM, Heath RB, Shiu SY. Congenital infection by human cytomegalovirus with a 65bp deletion in the morphological transforming region II. Arch Virol. 1993;129(1-4):295-9.
[11]Wright DK, Manos MM. Sample preparation from paraffin-embedded tissues. In: Inns MA, Gelfand DH, Srinsky JJ, White TJ. PCR protocols: A guide to method and applications. San Diego: Academic Press; 1990. p. 153-8.