@2024 Afarand., IRAN
ISSN: 2251-8215 Sarem Journal of Reproductive Medicine 2018;2(4):159-163
ISSN: 2251-8215 Sarem Journal of Reproductive Medicine 2018;2(4):159-163
Comparison of Serum Anti-Peroxiredoxin 3 and Anti-Peroxiredoxin 4 Auto Antibodies in the Patients with a History of Recurrent Spontaneous Abortion and Healthy Women
ARTICLE INFO
Article Type
Original ResearchAuthors
Roumandeh N. (1)Saremi A.T. (2)
Pooladi A. (2)
Sanaye Naderi M. (3)
Younesi B. (3)
Arasteh J. (4)
Zare A. (*)
(*) “Sarem Fertility & Infertility Research Center (SAFIR)” and “Sarem Cell Research Center (SCRC)” , Sarem Women’s Hospital, Tehran, Iran
(1) Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
(2) “Sarem Fertility & Infertility Research Center (SAFIR)” and “Sarem Cell Research Center (SCRC)” , Sarem Women’s Hospital, Tehran, Iran
(2) “Sarem Fertility & Infertility Research Center (SAFIR)” and “Sarem Cell Research Center (SCRC)” , Sarem Women’s Hospital, Tehran, Iran
(3) Laboratory of Sarem Women’s Hospital, Tehran, Iran
(4) Biology Department, Basic Sciences Faculty, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Correspondence
Article History
Received: April 13, 2017Accepted: September 20, 2017
ePublished: November 15, 2018
ABSTRACT
Aims
Recurrent spontaneous abortion (RSA) is one of the most common problems experienced during pregnancy. Immunologic factors, particularly autoimmunity, are contributing to the outcomes of pregnancy in the patients with RSA. Autoimmune antibodies can have a detrimental effect on the placenta and on the fetus and lead to abortion. Therefore, the objective of this study was to compare the serum levels of anti-peroxiredoxin 3 (Anti-Prx3) and anti-peroxiredoxin 4 (anti-Prx4) antibodies in the patients with a history of RSA and healthy women.
Materials & Methods This case-control study was conducted on 100 females with a history of at least three RSA as the case group and 32 healthy women with at least two successful pregnancies without any abortion as the control group in Sarem women hospital (Tehran, Iran). The serum levels of anti-Prx3 and anti-Prx4 were measured in the groups using ELISA method. The data were analyzed by Mann-Whitney U test using SPSS software.
Findings The level of anti-Prx4 in patients with RSA was significantly higher than control group (p=0.004). However, there was no statistically difference in the level of anti-Prx3 between patients with RSA and healthy women (p=0.51).
Conclusion The serum level of anti-Prx3 autoantibody in the patients with RSA and healthy women is not different, while the serum level of anti-Prx4 autoantibody in the patients with a history of RSA is higher than that of healthy women.
Materials & Methods This case-control study was conducted on 100 females with a history of at least three RSA as the case group and 32 healthy women with at least two successful pregnancies without any abortion as the control group in Sarem women hospital (Tehran, Iran). The serum levels of anti-Prx3 and anti-Prx4 were measured in the groups using ELISA method. The data were analyzed by Mann-Whitney U test using SPSS software.
Findings The level of anti-Prx4 in patients with RSA was significantly higher than control group (p=0.004). However, there was no statistically difference in the level of anti-Prx3 between patients with RSA and healthy women (p=0.51).
Conclusion The serum level of anti-Prx3 autoantibody in the patients with RSA and healthy women is not different, while the serum level of anti-Prx4 autoantibody in the patients with a history of RSA is higher than that of healthy women.
CITATION LINKS
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[7]Chaichian S, Shoaee S, Saremi A, Pedar S, Firouzi F. Factors influencing success rate of leukocyte immunization and anti-paternal antibodies in spontaneous recurrent miscarriage. Am J Reprod Immunol. 2007;57(3):169-76.
[8]Krechetova LV, Nikolaeva MA, Van'ko LV, Ziganshina MM, Golubeva EL, Stepanova EO, et al. Optimal detection of serum antipaternal antileukocytic antibodies after injection of allogenic lymphocytes in women with habitual abortions. Bull Exp Biol Med. 2012;153(5):726-9.
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[10]Wilczynski JR, Radwan P, Tchorzewski H, Banasik M. Immunotherapy of patients with recurrent spontaneous miscarriage and idiopathic infertility: Does the immunization-dependent Th2 cytokine overbalance really matter?. Arch Immunol Ther Exp. 2012;60(2):151-60.
[11]Beaman KD, Ntrivalas E, Mallers TM, Jaiswal MK, Kwak Kim J, Gilman Sachs A. Immune etiology of recurrent pregnancy loss and its diagnosis. Am J Reprod Immunol. 2012;67(4):319-25.
[12]Ford HB, Schust DJ. Recurrent pregnancy loss: Etiology, diagnosis and therapy. Rev Obstet Gynecol. 2009;2(2):76-83.
[13]Perricone C, De Carolis C, Perricone R. Pregnancy and autoimmunity: A common problem. Best Pract Res Clin Rheumatol. 2012;26(1):47-60.
[14]Christiansen OB. Reproductive immunology. Mol Immunol. 2013;55(1):8-15.
[15]Gupta S, Agarwal A, Banerjee J, Alvarez JG. The role of oxidative stress in spontaneous abortion and recurrent pregnancy loss: A systematic review. Obstet Gynecol Surv. 2007;62(5):335-47.
[16]Nelson KJ, Knutson ST, Soito L, Klomsiri C, Poole LB, Fetrow JS. Analysis of the peroxiredoxin family: Using active-site structure and sequence information for global classification and residue analysis. Proteins. 2011;79(3):947-64.
[17]Gharesi Fard B, Jafarzadeh L, Ghaderi shabankareh F, Zolghadri J, Kamali Sarvestani E. Presence of autoantibody against two placental proteins, peroxiredoxin 3 and peroxiredoxin 4, in sera of recurrent pregnancy loss patients. Am J Reprod Immunol. 2013;69(3):248-55.
[18]Cervera R, Balasch J. Autoimmunity and recurrent pregnancy losses. Clin Rev Allergy Immunol. 2010;39(3):148-52.
[19]Kwak Kim J, Agcaoili MS, Aleta L, Liao A, Ota K, Dambaeva S, et al. Management of women with recurrent pregnancy losses and antiphospholipid antibody syndrome. Am J Reprod Immunol. 2013;69(6):596-607.
[20]Kuon RJ, Strowitzki T, Sohn C, Daniel V, Toth B. Immune profiling in patients with recurrent miscarriage. J Reprod Immunol. 2015;108:136-41.
[21]Shoenfeld Y, Carp HJ, Molina V, Blank M, Cervera R, Balasch J, et al. Autoantibodies and prediction of reproductive failure. Am J Reprod Immunol. 2006;56(5-6):337-44.
[22]Karasawa R, Ozaki S, Nishioka K, Kato T. Autoantibodies to peroxiredoxin I and IV in patients with systemic autoimmune diseases. Microbiol Immunol. 2005;49(1):57-65.
[2]Garrido Gimenez C, Alijotas Reig J. Recurrent miscarriage: Causes, evaluation and management. Postgrad Med J. 2015;91(1073):151-62.
[3]Toth B, Jeschke U, Rogenhofer N, Scholz C, Wurfel W, Thaler CJ, et al. Recurrent miscarriage: Current concepts in diagnosis and treatment. J Reprod Immunol. 2010;85(1):25-32.
[4]Saito S, Nakashima A, Shima T, Ito M. Th1/Th2/Th17 and regulatory T-cell paradigm in pregnancy. Am J Reprod Immunol. 2010;63(6):601-10.
[5]Lee SK, Kim JY, Hur SE, Kim CJ, Na BJ, Lee M, et al. An imbalance in interleukin-17-producing T and Foxp3+ regulatory T cells in women with idiopathic recurrent pregnancy loss. Hum Reprod. 2011;26(11):2964-71.
[6]Sharma S. Natural killer cells and regulatory T cells in early pregnancy loss. Int J Dev Biol. 2014;58(2-4):219-29.
[7]Chaichian S, Shoaee S, Saremi A, Pedar S, Firouzi F. Factors influencing success rate of leukocyte immunization and anti-paternal antibodies in spontaneous recurrent miscarriage. Am J Reprod Immunol. 2007;57(3):169-76.
[8]Krechetova LV, Nikolaeva MA, Van'ko LV, Ziganshina MM, Golubeva EL, Stepanova EO, et al. Optimal detection of serum antipaternal antileukocytic antibodies after injection of allogenic lymphocytes in women with habitual abortions. Bull Exp Biol Med. 2012;153(5):726-9.
[9]Liang P, Mo M, Li GG, Yin B, Cai J, Wu T, et al. Comprehensive analysis of peripheral blood lymphocytes in 76 women with recurrent miscarriage before and after lymphocyte immunotherapy. Am J Reprod Immunol. 2012;68(2):164-74.
[10]Wilczynski JR, Radwan P, Tchorzewski H, Banasik M. Immunotherapy of patients with recurrent spontaneous miscarriage and idiopathic infertility: Does the immunization-dependent Th2 cytokine overbalance really matter?. Arch Immunol Ther Exp. 2012;60(2):151-60.
[11]Beaman KD, Ntrivalas E, Mallers TM, Jaiswal MK, Kwak Kim J, Gilman Sachs A. Immune etiology of recurrent pregnancy loss and its diagnosis. Am J Reprod Immunol. 2012;67(4):319-25.
[12]Ford HB, Schust DJ. Recurrent pregnancy loss: Etiology, diagnosis and therapy. Rev Obstet Gynecol. 2009;2(2):76-83.
[13]Perricone C, De Carolis C, Perricone R. Pregnancy and autoimmunity: A common problem. Best Pract Res Clin Rheumatol. 2012;26(1):47-60.
[14]Christiansen OB. Reproductive immunology. Mol Immunol. 2013;55(1):8-15.
[15]Gupta S, Agarwal A, Banerjee J, Alvarez JG. The role of oxidative stress in spontaneous abortion and recurrent pregnancy loss: A systematic review. Obstet Gynecol Surv. 2007;62(5):335-47.
[16]Nelson KJ, Knutson ST, Soito L, Klomsiri C, Poole LB, Fetrow JS. Analysis of the peroxiredoxin family: Using active-site structure and sequence information for global classification and residue analysis. Proteins. 2011;79(3):947-64.
[17]Gharesi Fard B, Jafarzadeh L, Ghaderi shabankareh F, Zolghadri J, Kamali Sarvestani E. Presence of autoantibody against two placental proteins, peroxiredoxin 3 and peroxiredoxin 4, in sera of recurrent pregnancy loss patients. Am J Reprod Immunol. 2013;69(3):248-55.
[18]Cervera R, Balasch J. Autoimmunity and recurrent pregnancy losses. Clin Rev Allergy Immunol. 2010;39(3):148-52.
[19]Kwak Kim J, Agcaoili MS, Aleta L, Liao A, Ota K, Dambaeva S, et al. Management of women with recurrent pregnancy losses and antiphospholipid antibody syndrome. Am J Reprod Immunol. 2013;69(6):596-607.
[20]Kuon RJ, Strowitzki T, Sohn C, Daniel V, Toth B. Immune profiling in patients with recurrent miscarriage. J Reprod Immunol. 2015;108:136-41.
[21]Shoenfeld Y, Carp HJ, Molina V, Blank M, Cervera R, Balasch J, et al. Autoantibodies and prediction of reproductive failure. Am J Reprod Immunol. 2006;56(5-6):337-44.
[22]Karasawa R, Ozaki S, Nishioka K, Kato T. Autoantibodies to peroxiredoxin I and IV in patients with systemic autoimmune diseases. Microbiol Immunol. 2005;49(1):57-65.