@2024 Afarand., IRAN

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ISSN: 2252-0805    The Horizon of Medical Sciences 2016;22(1):71-75

Background
… [1, 2] Lavender officinalis has antibacterial effects and its essence main compositions are linalil stat and linalool, geraniol, coumarin, flavonoids, borneol, strol, terpenoid hydrocarbons, butyric acid, propionic acid, and valeric acid [1, 3-6].
Previous Studies
The positive effects of Lavender officinalis on nervous cells, leading to reductions in pain and anxiety, have been confirmed. In addition, the herb has anti-psychotic and anti-depressant effects [7, 8]. The side effects of the extract of the flower of Lavender officinalis on the biochemical factors of the liver have not yet been studied. … [9]
Aim(s)
The aim of this study was to investigate the effects of hydro-alcoholic extract of Lavender officinalis on the enzymes and histology of the liver.
Research type
This is an experimental study.
Research society, place and time
Adult female small laboratory mice weighing 20-28g were studied in Payam-E-Noor University (Iran) in 2014.
Sampling method and number
50 mice were studied.
Used devices&materials
The mice were kept at standard cages (20-22°C and natural light cycle). Hydro-alcoholic extract was prepared from the flowers [10, 11].The mice were divided into 5 groups, each including 10 mice. The groups were control, placebo, and three treatment groups. Control group received no medical treatment. To be sure on no stress in placebo group, normal saline injection was done 10 times every other day. Treatment groups 1, 2, and 3 received 50, 100, and 200mg per kg of the hydro-alcoholic extract of Lavender officinalis as intra-peritoneal 10 times every other day, respectively. Blood sampling was done after 20 days. The slices of liver, kept in formalin, were used in histology via Hematoxylin-Eosin method. Data was analyzed, using SPSS 16 software and ANOVA. … [12-14]
Findings by Text
There was a significant increase in ALT enzyme in 200mg/kg treatment group (65.80±0.23unit per lit.) than control group (50.10±0.31unit per lit.). There was a significant reduction in AST enzyme in 100 (147.90±0.23unit per lit.) and 200mg/kg (149.80±0.19unit per lit.) treatment groups than control group (221.10±0.26unit per lit.). There was a significant reduction in ALP enzymes in 200mg/kg treatment group (149.60±0.36unit per lit.) than control (220.10±0.24unit per lit.) group (Diagram 1). Based on the histologic study, there was no pathologic effect in control group and the liver tissue was completely normal (Fig. 1a). The liver tissue in 50mg/kg treatment group had a normal appearance and there was no considerable pathologic change (Fig. 1b). Despite more concentrated blood cells and hyperemia in the central veins in the liver tissue of 100mg/kg treatment group, there was no trace of phagocytic vacuoles and pathologic effects (Fig. 1c). Nevertheless, there were many hyperemia events in the central veins in 200mg/kg treatment group, as well as basophil nuclear assemblies which were due to the destruction of the liver cells. In addition, there was a tissue necrosis due to the poison effect of the extract with 200mg/kg doses (Fig. 1d).
Main comparison to the similar studies
200mg/kg hydro-alcoholic extract of Lavender offivcinalis had an increasing effect on ALT enzyme. In contrast, 200mg/kg Melissa Extract reduces the concentration of the enzyme [15]. … [16] Linalool and its derivatives considerably reduce anxiety [17]. … [18-20] Based on the histologic results, 50 and 100mg/kg extract of Lavender officinalis had no significant pathologic effect. AMPK activators and the activity of the path affect suppression of cell proliferation [21]. Linalool and its ester derivatives have anti cell proliferation effects in the liver cells [14]. AMPK and PPARα activators affect anti-inflammatory and preserving reactions of Liver [18]. Extract of Lavender officinalis could suppress cell cycle, reduce cell life, and lead to liver tissue necrosis. MP suppression is via preventing HMGCR, which suppresses cell proliferation and, probably, cell activities of the liver cells. It, also probably, suppresses cholesterol synthesis [21].
Suggestions
Non-declared
Limitations
Non-declared
Conclusions
100 and 200mg/kg hydro-alcoholic extract of Lavender officinalis reduce AST and ALP enzymes. And alongside the dose increase up to 200mg/kg, there is severe necrosis in the liver tissue.
Acknowledgments
Staff of Biology Unit of Isfahan Payam-E-Noor University was appreciated.
Conflict of interest
Non-declared
Ethical Permissions
The study was confirmed by Ethics Committee of Isfahan Payam-E-Noor University. The study was based on the international rules on treating the laboratory animals.
Funding Sources
The study was funded by Isfahan Payam-E-Noor University.

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