ARTICLE INFO

Article Type

Original Research

Authors

Iziy   E. (1 )
Beheshti Nasr   S.M. (* )
Majd   A. (2 )






(* ) Cellular and Molecular Biology Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
(1 ) Traditional and Complementary Medicine Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
(2 ) Biology Department, Biological Sciences Faculty, Kharazmi University, Tehran, Iran

Correspondence

Address: Cell & Molecular Research Center, Medicine Faculty, No.2 Building, Sabzevar University of Medical Sciences, Asadabadi Street, Sabzevar, Iran
Phone: +985714446070 (246)
Fax: +985714445648
beheshti.m1985@gmail.com

Article History

Received:  December  13, 2013
Accepted:  June 7, 2014
ePublished:  July 1, 2014

BRIEF TEXT


Spartium junceum L. is a herb with shining and aromatic yellow flowers, planting as an ornamental flower [1-3]. Flowering time of the herb is springtime, in that time allergen compounds of the aromatic herbs hugely increase [1, 4, 5]. While connecting to the immunoglobulin E (IgE), allergen compounds result in degranulation induction of mast cells, and this results in wheal and feller reactions [11, 12].

In previous studies, intense allergenicity of the herb’s pollens and its very aromatic petals has been proved [1, 14, 15]; however there is no research about its petal allergenicity at old stage (stage without pollen).

The aim of this study was to investigate the allergenicity of petal and stamen at old ontogenical staged Spartium junceum L. in Guinea pig.

This study is experimental one.

Research society was the population of 350-500gr male Hartley Guinea pigs (Razi Vaccine and Serum Research Institute-Mashhad).

21 male Hartley Guinea pigs were selected.

Old ontogenical staged Spartium junceum L. were collected at spring (flowering time). 16% buffer extraction was produced from petals and stamens of old ontogenical stage in 0.1 molar neutral saline phosphate buffer solutions (PBS) and with pH 7.2 [1, 18].‏ The Guinea pigs were randomly divided into 3 groups of 7. The first group was without treatment (negative control with salt phosphate buffer injection). The second group was being treated with buffer extraction of old petals. The third group was being treated with buffer extract of old petals and stamens. There were 5 weeks injections, one time per week 100 micro liter of extraction as peritoneal [19], with subcutaneously last injection [1] in the groin. One hour after injection [20], skin reactions were evaluated through wheal (white bumps) and feller (red halo around the wheal) diameter measurements. Finally, one week after the last injection, blood samples were collected from the animals’ hearts. Immunoglobulin E (IgE) was measured in terms of IU/ml through ELIZA method and by the use of ELISA Kit for Guinea Pig (catalogue No. ET-151, RPC Company; Russia), and the number of eosinophils was measured by the use of CBC instrument, and blood sugar was measured in terms of mg/dl, and they were compared between various groups [21]. For identification of protein bands based on molecular weight, SDS-PAGE electrophoresis method in discontinuous system was used. SPSS 16 and One-way Variance Analysis were used in order to analyze data. To show difference between data, every desired parameter was compared with the related control, using Independent T test.

Microtome sections showed secretory structures in old petals. In addition, microscopic studies of stamens showed fully ripe and blown haploids, in such a case that they were without pollens. The wheals’ diameter, formed during skin test, increased in the two treated groups than control group did, which was significant. However, there was no significant difference between the two treated groups. Average blood immunoglobulin E and average blood eosinophils in the treated group with petal extract and the treated group with petal and stamen extract increased, than control group did. However, this increase was not statistically significant. Blood sugar in the treated group with petal extraction and the treated group with petal and stamen extraction increased, than control group did. This increase was significant in the treated group with petal and stamen extraction, only (Table 1). Electrophoretic profile of buffer extracts showed protein bands with 46, 54, and 85 kD molecular weight, with bold bands for petal extraction containing stamen, than petal extraction did. This difference may be resulted by increase in number of every protein or its concentration in petal extraction containing stamen (Fig. 1).

In the present research, in contrast to previous studies which had studied only pollen [1, 14], the old staged herb was selected and allergenicity of petal and pollen was studied. In addition, in contrast to another study [15], sample size, used in the present study, was increased to form better scientific judgment. Protein bands, caused by petal and stamen extract of Spartium junceum L and reported in the present study, are similar to the reported bands in some other studies [1, 4, 15]. Increase in feller and wheal’s diameter, IgE, and eosinophils, shown in the present study, is consistent with results of some other studies [1, 14, 27-30].

It is suggested that planting the herb around places currently occupied by children and old persons, as well as hospitals, to be decreased.

Small sample size and special conditions of keeping Guinea pigs were the study limits.

Old ontogenical staged Spartium junceum L. is allergic for Guinea pigs; and allergenicity of its petal extract including stamen is more than petal extract alone does.

Researchers feel grateful to Technology and Research Deputy of Kharazmi University, Tehran, Iran.

Non-declared

Non-declared

Technology and Research Deputy of Kharazmi University funded the study.

TABLES and CHARTS

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