@2024 Afarand., IRAN

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ISSN: 2252-0805    The Horizon of Medical Sciences 2016;22(2):145-150

Background
Propolis is one of the most natural products derived from plant resin that the bees add some discharges after collecting it [1, 2]. … [3-14]
Previous Studies
Propolis properties include antibacterial antifungal, anti-parasitic, anti-inflammatory, and teeth anti-caries. Also, it is known as booster of body immune system, mouth and gum disorders promoter and a local anesthetic [15-18]. … [20]
Aim(s)
The aim of this study was to determine the effect of methanol extract of propolis of Dasht-e-Ghazvin area on some biochemical parameters and serum antioxidant capacity in adult male rats.
Research type
This is an experimental research.
Research society, place and time
250-300 g Wistar rats were studied.
Sampling method and number
40 rats were studied.
Used devices&materials
30 g propolis was collected from Dasht-e-Ghazvin area. Methanol extract was filtered by Whatman-42. Rats were classified in four groups of 10 animals as follows: control group which received only distilled water and propylene alcohol 0.5 ml as gavage and experimental groups which received 50, 100 and 200 mg/kg propolis. The groups were exposed to gavage every other day for a 10-day period. Blood sampled were collected after 24 hours of fasting. Levels of glucose, TG, AST and ALT were evaluated using enzymes diagnostic kits (Parsazmoon Co.; Iran). Serum total antioxidant capacity was measured using FRAP (antioxidant capacity reduction of iron) method (Sigman; USA). For making FRAP solution, also, the compound of 10 ml of acetate buffer, one ml of TPTZ solution, and one ml of ferric chloride were used. The standard curve was plotted using standard solution and according to the mentioned method and through it, the amount of antioxidant capacity in the samples was calculated [21]. The obtained data was studied and analyzed by SPSS 20 software using one-way ANOVA test and Tukey`s post-hoc test.
Findings by Text
Blood sugar levels in different groups of propolis did not have any significant difference with control group (p > 0.05). Serum triglyceride levels in mice exposed to the gavage with 100 mg/kg of propolis had significant decrease compared to control group (p < 0.001). Also, the serum level of aspartate aminotransferase did not show significant increase in the groups received 100 mg/kg (p < 0.01) and 200 mg/kg (p < 0.001) of propolis comparing control group. Alanine aminotransferase serum levels significantly decreased in propolis group received 50 mg/kg compared to control group (p < 0.001), but its rate increased in the group received 200 mg/kg (p < 0.01). Propolis, also, significantly increased serum antioxidant capacity, particularly at 50 mg/kg (p < 0.05), and 100 mg/kg (p < 0.01) compared to control group (Table 1).
Main comparison to the similar studies
Propolis administration using the doses, reduced fasting blood sugar, however it was not significant, but it changed the serum levels of triglycerides, aspartate aminotransferase and alanine aminotransferase significantly. In this study, 100 mg/kg propolis administration reduced the level of triglycerides serums compared to control group. Also, the gradual increase in the concentration of propolis up to 200 mg/kg significantly increased the serum level of both aspartate aminotransferase and alanine aminotransferase enzymes except in 50 mg/kg. Propetamphos causes abnormal changes in serum levels of glucose, triglycerides, and activity of liver aspartate aminotransferase, and alanine aminotransferase of mice received it, and by administration of prospolis, the glucose and triglyceride become normal to some extent. However, the levels of liver enzymes become higher [17]. … [22] The finding is consistent with the results of our study. In Wistar rats under toxicity with aluminum chloride, propolis could prevent any increase in the level of glucose, total fat, triglyceride and cholesterol to some extent [23]. In mice treated with sodium fluoride, which had a longer period of receiving propolis, an increase of aminotransferase and a decrease in blood sugar and triglycerides were observed [24]. The results of that study are consistent with our study in an increase in the level of aminotransferase and a decrease in the level of triglycerides. Propolis consumption with 50 and 100 mg/kg concentrations significantly increased total antioxidant capacity in serum compared to control group. In a similar study, the effect of propolis on the levels of lipid peroxidation caused by chloride aluminum was investigated and the serum levels of antioxidant increased, and the activity of glutathione S-transferase, superoxide dismutase, catalase, and glutathione peroxidase in the liver, kidney and brain of the mice being treated with chloride aluminum decreased, whereas in the mice treated with propolis, the level of serum oxidants decreased and the antioxidant enzyme level was increased [23]. In the other study, the administration of sodium fluoride in the mice has reduced the antioxidant level. However, in the groups which received proplis in addition to fluoride, the level of catalase has increased [24]. … [25] The administration of propolis improves oxidative condition and lipid metabolism in alloxan-induced diabetic rats [26]. … [27-30] Propolis is capable to reduce brain total antioxidant and anti-oxidation enzymes, and increase the brain tissue malondialdehyde in the chronic stress condition [31]. The results of these studies are consistent with the present findings regarding the increase of serum total antioxidant capacity following administration of propolis. … [32-34]
Suggestions
Anti-apoptotic and anti-cancer effects of propolis of each geographic area should be studied.
Limitations
There was no important limitation in this study.
Conclusions
Oral administration of Methanol extract of propolis of Dasht-e-Qazvin area shows a moderating effect on some biochemical factors of the blood biochemical factors and particularly triglycerides and increases total antioxidant capacity of the serum. Nevertheless, its high consumption may be harmful in patients with liver diseases.
Acknowledgments
Growth and Biochemical Technology Center and Medical and Paramedical Schools of Qazvin University of Medical Sciences are appreciated.
Conflict of interest
Non-declared
Ethical Permissions
This study has been approved by Ethics Committee of Qazvin University of Medical Sciences.
Funding Sources
The article is a result of a research project of Biochemical Research and Technology Development Center of Qazvin University of Medical Sciences.

TABLES and CHARTS

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